Restriction map for pF1KE1073
 (Linear) (Six-base) MAPSORT of: KIEE1073.seq  Check: 6949  from: 1  to: 2433



KIEE1073.orf

 With 182 enzymes: SgfI * 

                        February 15, 2010 17:50  ..

AccI GT'mk_AC

Cuts at:      0      65     111     320    2433 
   Size:         65      46     209    2113

  Fragments arranged by size: 

                2113     209      65      46
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     287     550     581     769     866    2260    2335    2433 
   Size:        287     263      31     188      97    1394      75      98

  Fragments arranged by size: 

                1394     287     263     188      98      97      75      31
            
 
AflII C'TTAA_G

Cuts at:      0    1774    2433 
   Size:       1774     659
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     143    2433 
   Size:        143    2290
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1049    1081    2433 
   Size:       1049      32    1352

  Fragments arranged by size: 

                1352    1049      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1819    2433 
   Size:       1819     614
 
ApaLI G'TGCA_C

Cuts at:      0    1412    2433 
   Size:       1412    1021
 
ApoI r'AATT_y

Cuts at:      0     287    1697    2433 
   Size:        287    1410     736

  Fragments arranged by size: 

                1410     736     287
 
AvaI C'yCGr_G

Cuts at:      0    1688    2433 
   Size:       1688     745
 
BamHI G'GATC_C

Cuts at:      0    2116    2433 
   Size:       2116     317
 
BanII G_rGCy'C

Cuts at:      0      80    1067    2294    2433 
   Size:         80     987    1227     139

  Fragments arranged by size: 

                1227     987     139      80
 
BclI T'GATC_A

Cuts at:      0     180    2433 
   Size:        180    2253
 
BfrBI ATG'CAT

Cuts at:      0    1951    2433 
   Size:       1951     482
 
BlpI GC'TnA_GC

Cuts at:      0    1790    2433 
   Size:       1790     643
 
Bme1580I G_kGCm'C

Cuts at:      0    1416    1689    2433 
   Size:       1416     273     744

  Fragments arranged by size: 

                1416     744     273
 
BmgBI CAC'GTC

Cuts at:      0     324    2433 
   Size:        324    2109
 
BmtI G_CTAG'C

Cuts at:      0    1486    2433 
   Size:       1486     947
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1051    2433 
   Size:       1051    1382
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1816    2122    2433 
   Size:       1816     306     311

  Fragments arranged by size: 

                1816     311     306
 
BsaAI yAC'GTr

Cuts at:      0    2406    2433 
   Size:       2406      27
 
BsaWI w'CCGG_w

Cuts at:      0     295    2433 
   Size:        295    2138
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     308     338    1049    1079    2433 
   Size:        308      30     711      30    1354

  Fragments arranged by size: 

                1354     711     308      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1654    2433 
   Size:       1654     779
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1149    2218    2433 
   Size:       1149    1069     215

  Fragments arranged by size: 

                1149    1069     215
 
BsiEI CG_ry'CG

Cuts at:      0      93    2433 
   Size:         93    2340
 
BsiHKAI G_wGCw'C

Cuts at:      0    1067    1139    1416    2294    2433 
   Size:       1067      72     277     878     139

  Fragments arranged by size: 

                1067     878     277     139      72
 
BsmI GAATG_Cn'

Cuts at:      0    1195    1953    1969    2433 
   Size:       1195     758      16     464

  Fragments arranged by size: 

                1195     758     464      16
 
Bsp1286I G_dGCh'C

Cuts at:      0      80    1067    1139    1416    1689    2294    2433 
   Size:         80     987      72     277     273     605     139

  Fragments arranged by size: 

                 987     605     277     273     139      80      72
 
BsrBI CCG'CTC

Cuts at:      0      95     413    1740    1800    2433 
   Size:         95     318    1327      60     633

  Fragments arranged by size: 

                1327     633     318      95      60
 
BsrDI GCAATG_nn'

Cuts at:      0    1751    2433 
   Size:       1751     682
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1729    2433 
   Size:       1729     704
 
BstBI TT'CG_AA

Cuts at:      0    1455    2433 
   Size:       1455     978
 
BstYI r'GATC_y

Cuts at:      0    2116    2433 
   Size:       2116     317
 
Bsu36I CC'TnA_GG

Cuts at:      0     772    2433 
   Size:        772    1661
 
BtgI C'CryG_G

Cuts at:      0     351    2433 
   Size:        351    2082
 
BtsI GCAGTG_nn'

Cuts at:      0    1329    2433 
   Size:       1329    1104
 
ClaI AT'CG_AT

Cuts at:      0    1619    2433 
   Size:       1619     814
 
DraI TTT'AAA

Cuts at:      0    1394    2156    2433 
   Size:       1394     762     277

  Fragments arranged by size: 

                1394     762     277
 
DraIII CAC_nnn'GTG

Cuts at:      0     283    2433 
   Size:        283    2150
 
EaeI y'GGCC_r

Cuts at:      0      14      90    2417    2433 
   Size:         14      76    2327      16

  Fragments arranged by size: 

                2327      76      16      14
 
EagI C'GGCC_G

Cuts at:      0      90    2433 
   Size:         90    2343
 
EarI CTCTTCn'nnn_

Cuts at:      0    1763    2168    2433 
   Size:       1763     405     265

  Fragments arranged by size: 

                1763     405     265
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1821    2433 
   Size:       1821     612
 
EcoICRI GAG'CTC

Cuts at:      0    1065    2292    2433 
   Size:       1065    1227     141

  Fragments arranged by size: 

                1227    1065     141
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     287     550     581     769     866    1051    2260    2335 
   Size:        287     263      31     188      97     185    1209      75

Cuts at:   2335    2433 
   Size:         98

  Fragments arranged by size: 

                1209     287     263     188     185      98      97      75
                  31
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1405    1789    2433 
   Size:       1405     384     644

  Fragments arranged by size: 

                1405     644     384
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     240     272    2433 
   Size:        240      32    2161

  Fragments arranged by size: 

                2161     240      32
 
HincII GTy'rAC

Cuts at:      0     112     450    1873    2433 
   Size:        112     338    1423     560

  Fragments arranged by size: 

                1423     560     338     112
 
HindIII A'AGCT_T

Cuts at:      0    1082    1246    2433 
   Size:       1082     164    1187

  Fragments arranged by size: 

                1187    1082     164
 
MfeI C'AATT_G

Cuts at:      0    1600    2433 
   Size:       1600     833
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     524    1017    2433 
   Size:        524     493    1416

  Fragments arranged by size: 

                1416     524     493
 
MscI TGG'CCA

Cuts at:      0      16    2419    2433 
   Size:         16    2403      14

  Fragments arranged by size: 

                2403      16      14
 
MslI CAynn'nnrTG

Cuts at:      0     746    2433 
   Size:        746    1687
 
MspA1I CmG'CkG

Cuts at:      0     107     570    2149    2433 
   Size:        107     463    1579     284

  Fragments arranged by size: 

                1579     463     284     107
 
NcoI C'CATG_G

Cuts at:      0     351    2433 
   Size:        351    2082
 
NheI G'CTAG_C

Cuts at:      0    1482    2433 
   Size:       1482     951
 
NsiI A_TGCA'T

Cuts at:      0    1953    2433 
   Size:       1953     480
 
NspI r_CATG'y

Cuts at:      0     680    2433 
   Size:        680    1753
 
PfoI T'CCnGG_A

Cuts at:      0    1634    2433 
   Size:       1634     799
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1049    1081    2433 
   Size:       1049      32    1352

  Fragments arranged by size: 

                1352    1049      32
 
PsiI TTA'TAA

Cuts at:      0     238    2433 
   Size:        238    2195
 
PvuII CAG'CTG

Cuts at:      0    2149    2433 
   Size:       2149     284
 
SacI G_AGCT'C

Cuts at:      0    1067    2294    2433 
   Size:       1067    1227     139

  Fragments arranged by size: 

                1227    1067     139
 
SalI G'TCGA_C

Cuts at:      0     110    2433 
   Size:        110    2323
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1763    2433 
   Size:       1763     670
 
ScaI AGT'ACT

Cuts at:      0    1665    2433 
   Size:       1665     768
 
SfcI C'TryA_G

Cuts at:      0     514    1334    2433 
   Size:        514     820    1099

  Fragments arranged by size: 

                1099     820     514
 
SmlI C'TyrA_G

Cuts at:      0    1774    1795    2101    2433 
   Size:       1774      21     306     332

  Fragments arranged by size: 

                1774     332     306      21
 
SphI G_CATG'C

Cuts at:      0     680    2433 
   Size:        680    1753
 
StuI AGG'CCT

Cuts at:      0     523    2433 
   Size:        523    1910
 
StyI C'CwwG_G

Cuts at:      0      11     351    2433 
   Size:         11     340    2082

  Fragments arranged by size: 

                2082     340      11
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     344     601    2433 
   Size:        344     257    1832

  Fragments arranged by size: 

                1832     344     257
 
TatI w'GTAC_w

Cuts at:      0     479    1663    2226    2433 
   Size:        479    1184     563     207

  Fragments arranged by size: 

                1184     563     479     207
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     308     340    2433 
   Size:        308      32    2093

  Fragments arranged by size: 

                2093     308      32
 
XmnI GAAnn'nnTTC

Cuts at:      0     813    2433 
   Size:        813    1620
 

 Enzymes that do cut:

     AccI     AcuI    AflII     AlfI     AloI    AlwNI    ApaLI     ApoI
     AvaI    BamHI    BanII     BclI    BfrBI     BlpI Bme1580I    BmgBI
     BmtI     BpmI    BpuEI    BsaAI    BsaWI    BsaXI    BseRI     BsgI
    BsiEI  BsiHKAI     BsmI Bsp1286I    BsrBI    BsrDI   BstAPI    BstBI
    BstYI   Bsu36I     BtgI     BtsI     ClaI     DraI   DraIII     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI     FalI    Hin4I   HincII
  HindIII     MfeI     MmeI     MscI     MslI   MspA1I     NcoI     NheI
     NsiI     NspI     PfoI     PpiI     PsiI    PvuII     SacI     SalI
     SapI     ScaI     SfcI     SmlI     SphI     StuI     StyI    TaqII
     TatI     TstI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI   AflIII     AgeI     AhdI
     AleI     ApaI     AscI     AseI    AsiSI    AvrII     BaeI     BanI
     BbeI     BbsI    BbvCI     BcgI    BciVI     BglI    BglII     BmrI
     BplI   Bpu10I     BsaI    BsaBI    BsaHI    BseYI    BsiWI    BsmBI
    BspEI    BspHI    BspMI    BsrFI    BsrGI   BssHII    BssSI   BstEII
    BstXI  BstZ17I    BtgZI    CspCI     DrdI    EcoNI EcoO109I    EcoRI
    EcoRV     FseI     FspI    FspAI    HaeII     HpaI     KasI     KpnI
     MluI     NaeI     NarI     NdeI   NgoMIV     NotI     NruI     PacI
     PasI     PciI    PflMI     PmeI     PmlI    PpuMI    PshAI   PspOMI
    PspXI     PsrI     PstI     PvuI    RsrII    SacII    SanDI     SbfI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SrfI     SspI     SwaI  Tth111I     XbaI     XcmI     XhoI     XmaI
     ZraI
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