Restriction map for pF1KE1075
##################################
# NO CUT ENZYMES (  126 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AgeI        Aor51HI     
ApaI        AscI        Asp718I     AspI        AviII       
BalI        BamHI       BanI        BanII       BbeI        
BbrPI       BbvCI       BcgI        BciVI       BfrI        
BglI        BglII       BlpI        Bpu1102I    BsaAI       
BsaBI       BsaHI       BsaWI       BseAI       BseRI       
BsiEI       BsiWI       BsmBI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrFI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstYI       BstZ17I     CelII       
Cfr10I      CpoI        DraII       DraIII      DrdI        
EaeI        EagI        EclXI       Eco47III    Eco52I      
EcoNI       EcoO109I    EcoO65I     EcoRI       EcoRV       
FauI        FseI        FspI        HgaI        Hin1I       
HpaI        KasI        KpnI        KspI        MamI        
MflI        MluI        MluNI       MroI        MscI        
MvnI        NaeI        NarI        NdeI        NgoMIV      
NheI        NotI        NruI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       Psp1406I    PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SbfI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmlI        SnaBI       SpeI        Sse8387I    
StuI        SwaI        Tth111I     XbaI        XhoI        
XhoII       


###################################
# ONE CUT ENZYMES (   54 enzymes) #
###################################

AciI         1453  GCGG                 CCGC(-3/-1)
AflIII        850  ACACGT               A^C[GA][CT]GT
AhdI            7  GACCCGGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       1005  GTGCAC               G^TGCAC
AlwI          511  GGATC                GGATC(4/5)
AlwNI        1019  CAGCTCCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI           49  CCTGAGG              CC^T[ACGT]AGG
ApaLI        1005  GTGCAC               G^TGCAC
Asp700I      1222  GAAAATTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI           7  GACCCGGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI        1005  GTGCAC               G[AT]GC[AT]^C
AvaI            9  CCCGGG               C^[CT]CG[GA]G
AvrII        1804  CCTAGG               C^CTAGG
BbvI         1471  GCTGC                GCAGC(8/12)
BlnI         1804  CCTAGG               C^CTAGG
BmyI         1005  GTGCAC               G[AGT]GC[ACT]^C
BsgI         1025  CTGCAC               GTGCAG(16/14)
BsiHKAI      1005  GTGCAC               G[AT]GC[AT]^C
BsmI          930  GAATGC               GAATGC(1/-1)
BsoBI           9  CCCGGG               C^[CT]CG[GA]G
Bsp1286I     1005  GTGCAC               G[AGT]GC[ACT]^C
Bsp1407I     2152  TGTACA               T^GTACA
BsrDI        2097  CATTGC               GCAATG(2/0)
BsrGI        2152  TGTACA               T^GTACA
BstNI        1185  CCTGG                CC^[AT]GG
BstXI         400  CCAGATTGTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I         49  CCTGAGG              CC^T[ACGT]AGG
CfoI          170  GCGC                 GCG^C
Eam1105I        7  GACCCGGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I         49  CCTGAGG              CC^T[ACGT]AGG
EcoRII       1185  CCTGG                ^CC[AT]GG
HaeII         169  AGCGCC               [GA]GCGC^[CT]
HapII          10  CCGG                 C^CGG
HhaI          170  GCGC                 GCG^C
HinP1I        170  GCGC                 G^CGC
HpaII          10  CCGG                 C^CGG
MspA1I         41  CAGCTG               C[AC]G^C[GT]G
MspI           10  CCGG                 C^CGG
MvaI         1185  CCTGG                CC^[AT]GG
MwoI         1471  GCTGCTACTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PleI           61  GAGTC                GAGTC(4/5)
PstI          291  CTGCAG               CTGCA^G
PvuII          41  CAGCTG               CAG^CTG
SapI         1798  GCTCTTC              GCTCTTC(1/4)
ScaI         1611  AGTACT               AGT^ACT
SmaI            9  CCCGGG               CCC^GGG
SphI         1488  GCATGC               GCATG^C
SspBI        2152  TGTACA               T^GTACA
TseI         1471  GCTGC                G^C[AT]GC
Tsp45I       1275  GTCAC                ^GT[GC]AC
XcmI         1679  CCATAAGAGCTTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI           9  CCCGGG               C^CCGGG
XmaI            9  CCCGGG               C^CCGGG
XmnI         1222  GAAAATTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AseI         1309  ATTAAT               AT^TAAT
             1558  ATTAAT              
AsnI         1309  ATTAAT               AT^TAAT
             1558  ATTAAT              
AvaII         109  GGACC                G^G[AT]CC
             1441  GGACC               
BbsI           82  GAAGAC               GAAGAC(2/6)
             2323  GTCTTC              
BclI         1667  TGATCA               T^GATCA
             1742  TGATCA              
BcnI            9  CCCGG                CC^[GC]GG
               10  CCGGG               
BpuAI          82  GAAGAC               GAAGAC(2/6)
             2323  GTCTTC              
BsaI            5  GAGACC               GGTCTC(1/5)
              117  GAGACC              
BsmAI           5  GAGAC                GTCTC(1/5)
              117  GAGAC               
BsmFI        1440  GGGAC                GGGAC(10/14)
               34  GTCCC               
BspDI         136  ATCGAT               AT^CGAT
             1262  ATCGAT              
ClaI          136  ATCGAT               AT^CGAT
             1262  ATCGAT              
DsaI          645  CCATGG               C^C[GA][CT]GG
             1939  CCATGG              
EcoT22I      1490  ATGCAT               ATGCA^T
             1510  ATGCAT              
FbaI         1667  TGATCA               T^GATCA
             1742  TGATCA              
Fnu4HI       1453  GCGGC                GC^[ACGT]GC
             1471  GCTGC               
HaeIII         46  GGCC                 GG^CC
             1916  GGCC                
HindIII       599  AAGCTT               A^AGCTT
             1378  AAGCTT              
ItaI         1453  GCGGC                GC^[ACGT]GC
             1471  GCTGC               
MaeII         852  ACGT                 A^CGT
             1674  ACGT                
MfeI         1270  CAATTG               C^AATTG
             2338  CAATTG              
MslI          529  CATGTCCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              984  CACCCTAGTG          
MunI         1270  CAATTG               C^AATTG
             2338  CAATTG              
NciI            9  CCCGG                CC^[GC]GG
               10  CCGGG               
NcoI          645  CCATGG               C^CATGG
             1939  CCATGG              
NsiI         1490  ATGCAT               ATGCA^T
             1510  ATGCAT              
PflMI         542  CCAAGGAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2277  CCACAGGTTGG         
PshBI        1309  ATTAAT               AT^TAAT
             1558  ATTAAT              
Van91I        542  CCAAGGAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2277  CCACAGGTTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   49 enzymes) #
#########################################

AcsI        AfaI        AluI        ApoI        BfaI        
BpmI        BsaJI       BsiYI       BslI        BsrI        
BssKI       BstF5I      Cac8I       Cfr13I      DdeI        
DpnII       DraI        EarI        EcoT14I     FokI        
HincII      HindII      HinfI       HphI        Ksp632I     
MaeI        MaeIII      MboI        MboII       MnlI        
MseI        NdeII       NlaIII      NlaIV       NspI        
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SspI        StyI        TaqI        TfiI        
Tru9I       Tsp509I     TspRI       TthHB8I     
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