Restriction map for pF1KE1075
 (Linear) (Six-base) MAPSORT of: KIEE1075.seq  Check: 7376  from: 1  to: 2361



KIEE1075.orf

 With 182 enzymes: SgfI * 

                        February 15, 2010 17:50  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2135    2361 
   Size:       2135     226
 
AflIII A'CryG_T

Cuts at:      0     850    2361 
   Size:        850    1511
 
AhdI GACnn_n'nnGTC

Cuts at:      0      12    2361 
   Size:         12    2349
 
AleI CACnn'nnGTG

Cuts at:      0     988    2361 
   Size:        988    1373
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1024    2361 
   Size:       1024    1337
 
ApaLI G'TGCA_C

Cuts at:      0    1005    2361 
   Size:       1005    1356
 
ApoI r'AATT_y

Cuts at:      0     653    1146    1224    1337    1603    1695    1789    1979 
   Size:        653     493      78     113     266      92      94     190

Cuts at:   1979    2017    2208    2361 
   Size:         38     191     153

  Fragments arranged by size: 

                 653     493     266     191     190     153     113      94
                  92      78      38
 
AseI AT'TA_AT

Cuts at:      0    1310    1559    2361 
   Size:       1310     249     802

  Fragments arranged by size: 

                1310     802     249
 
AvaI C'yCGr_G

Cuts at:      0       9    2361 
   Size:          9    2352
 
AvrII C'CTAG_G

Cuts at:      0    1804    2361 
   Size:       1804     557
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    2285    2318    2361 
   Size:       2285      33      43

  Fragments arranged by size: 

                2285      43      33
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      89    2316    2361 
   Size:         89    2227      45

  Fragments arranged by size: 

                2227      89      45
 
BclI T'GATC_A

Cuts at:      0    1667    1742    2361 
   Size:       1667      75     619

  Fragments arranged by size: 

                1667     619      75
 
BfrBI ATG'CAT

Cuts at:      0    1492    1512    2361 
   Size:       1492      20     849

  Fragments arranged by size: 

                1492     849      20
 
Bme1580I G_kGCm'C

Cuts at:      0    1009    2361 
   Size:       1009    1352
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    2224    2361 
   Size:       2224     137
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      23     985    1220    2361 
   Size:         23     962     235    1141

  Fragments arranged by size: 

                1141     962     235      23
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     111    2361 
   Size:        111    2250
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1010    2361 
   Size:       1010    1351
 
BsiHKAI G_wGCw'C

Cuts at:      0    1009    2361 
   Size:       1009    1352
 
BsmI GAATG_Cn'

Cuts at:      0     936    2361 
   Size:        936    1425
 
Bsp1286I G_dGCh'C

Cuts at:      0    1009    2361 
   Size:       1009    1352
 
BsrDI GCAATG_nn'

Cuts at:      0    2096    2361 
   Size:       2096     265
 
BsrGI T'GTAC_A

Cuts at:      0    2152    2361 
   Size:       2152     209
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     407    2361 
   Size:        407    1954
 
Bsu36I CC'TnA_GG

Cuts at:      0      50    2361 
   Size:         50    2311
 
BtgI C'CryG_G

Cuts at:      0     645    1939    2361 
   Size:        645    1294     422

  Fragments arranged by size: 

                1294     645     422
 
BtsI GCAGTG_nn'

Cuts at:      0     288    2361 
   Size:        288    2073
 
ClaI AT'CG_AT

Cuts at:      0     137    1263    2361 
   Size:        137    1126    1098

  Fragments arranged by size: 

                1126    1098     137
 
DraI TTT'AAA

Cuts at:      0     236     845    1052    1109    1256    1319    1700    1761 
   Size:        236     609     207      57     147      63     381      61

Cuts at:   1761    1985    2361 
   Size:        224     376

  Fragments arranged by size: 

                 609     381     376     236     224     207     147      63
                  61      57
 
EarI CTCTTCn'nnn_

Cuts at:      0     920    1537    1805    2361 
   Size:        920     617     268     556

  Fragments arranged by size: 

                 920     617     556     268
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      23     985    1220    2135    2361 
   Size:         23     962     235     915     226

  Fragments arranged by size: 

                 962     915     235     226      23
 
HaeII r_GCGC'y

Cuts at:      0     173    2361 
   Size:        173    2188
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      30     270     302     946     978    1276    1308    2173 
   Size:         30     240      32     644      32     298      32     865

Cuts at:   2173    2205    2361 
   Size:         32     156

  Fragments arranged by size: 

                 865     644     298     240     156      32      32      32
                  32      30
 
HincII GTy'rAC

Cuts at:      0      65    1067    2315    2361 
   Size:         65    1002    1248      46

  Fragments arranged by size: 

                1248    1002      65      46
 
HindIII A'AGCT_T

Cuts at:      0     599    1378    2361 
   Size:        599     779     983

  Fragments arranged by size: 

                 983     779     599
 
MfeI C'AATT_G

Cuts at:      0    1270    2338    2361 
   Size:       1270    1068      23

  Fragments arranged by size: 

                1270    1068      23
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     388    2361 
   Size:        388    1973
 
MslI CAynn'nnrTG

Cuts at:      0     533     988    2361 
   Size:        533     455    1373

  Fragments arranged by size: 

                1373     533     455
 
MspA1I CmG'CkG

Cuts at:      0      43    2361 
   Size:         43    2318
 
NcoI C'CATG_G

Cuts at:      0     645    1939    2361 
   Size:        645    1294     422

  Fragments arranged by size: 

                1294     645     422
 
NsiI A_TGCA'T

Cuts at:      0    1494    1514    2361 
   Size:       1494      20     847

  Fragments arranged by size: 

                1494     847      20
 
NspI r_CATG'y

Cuts at:      0     532    1460    1492    2361 
   Size:        532     928      32     869

  Fragments arranged by size: 

                 928     869     532      32
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     548    2283    2361 
   Size:        548    1735      78

  Fragments arranged by size: 

                1735     548      78
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     474     506    2361 
   Size:        474      32    1855

  Fragments arranged by size: 

                1855     474      32
 
PstI C_TGCA'G

Cuts at:      0     295    2361 
   Size:        295    2066
 
PvuII CAG'CTG

Cuts at:      0      43    2361 
   Size:         43    2318
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1805    2361 
   Size:       1805     556
 
ScaI AGT'ACT

Cuts at:      0    1613    2361 
   Size:       1613     748
 
SfcI C'TryA_G

Cuts at:      0     291     965    1070    1391    2288    2361 
   Size:        291     674     105     321     897      73

  Fragments arranged by size: 

                 897     674     321     291     105      73
 
SmaI CCC'GGG

Cuts at:      0      11    2361 
   Size:         11    2350
 
SphI G_CATG'C

Cuts at:      0    1492    2361 
   Size:       1492     869
 
SspI AAT'ATT

Cuts at:      0     726     918    1135    1325    1647    2361 
   Size:        726     192     217     190     322     714

  Fragments arranged by size: 

                 726     714     322     217     192     190
 
StyI C'CwwG_G

Cuts at:      0     542     645    1804    1939    2361 
   Size:        542     103    1159     135     422

  Fragments arranged by size: 

                1159     542     422     135     103
 
TatI w'GTAC_w

Cuts at:      0    1611    2152    2361 
   Size:       1611     541     209

  Fragments arranged by size: 

                1611     541     209
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1935    1967    2361 
   Size:       1935      32     394

  Fragments arranged by size: 

                1935     394      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1686    2361 
   Size:       1686     675
 
XmaI C'CCGG_G

Cuts at:      0       9    2361 
   Size:          9    2352
 
XmnI GAAnn'nnTTC

Cuts at:      0    1226    2361 
   Size:       1226    1135
 

 Enzymes that do cut:

     AcuI   AflIII     AhdI     AleI    AlwNI    ApaLI     ApoI     AseI
     AvaI    AvrII     BaeI     BbsI     BclI    BfrBI Bme1580I     BmrI
     BpmI     BsaI     BsgI  BsiHKAI     BsmI Bsp1286I    BsrDI    BsrGI
    BstXI   Bsu36I     BtgI     BtsI     ClaI     DraI     EarI  Eco57MI
    HaeII    Hin4I   HincII  HindIII     MfeI     MmeI     MslI   MspA1I
     NcoI     NsiI     NspI    PflMI     PsrI     PstI    PvuII     SapI
     ScaI     SfcI     SmaI     SphI     SspI     StyI     TatI     TstI
     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AlfI     AloI     ApaI     AscI    AsiSI    BamHI     BanI    BanII
     BbeI    BbvCI     BcgI    BciVI     BglI    BglII     BlpI    BmgBI
     BmtI     BplI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI    BsiEI    BsiWI    BsmBI    BspEI    BspHI
    BspMI    BsrBI    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstYI  BstZ17I    BtgZI    CspCI   DraIII     DrdI     EaeI     EagI
     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI     HpaI     KasI     KpnI     MluI     MscI     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     PacI     PasI
     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmlI    SnaBI
     SpeI     SrfI     StuI     SwaI    TaqII  Tth111I     XbaI     XhoI
     ZraI
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