Restriction map for pF1KE1096
##################################
# NO CUT ENZYMES (  136 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwI        AlwNI       AocI        ApaLI       
ApoI        AscI        Asp700I     Asp718I     AspEI       
AspI        AvrII       BalI        BamHI       BbeI        
BbrPI       BbvCI       BcgI        BciVI       BclI        
BfrI        BglI        BglII       BlnI        BpmI        
BsaBI       BsaI        BsaWI       BseAI       BsiEI       
BsiWI       BsmBI       BsmI        BspDI       BspEI       
BspHI       BspLU11I    BsrBI       BsrDI       BssHII      
Bst1107I    BstAPI      BstYI       BstZ17I     Bsu36I      
ClaI        CpoI        DraI        DraII       DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco52I      
Eco81I      EcoO109I    EcoRI       EcoRV       EcoT22I     
FbaI        FseI        HincII      HindII      HindIII     
HpaI        KasI        KpnI        KspI        MamI        
MfeI        MflI        MluI        MluNI       MroI        
MscI        MunI        NaeI        NarI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       Psp1406I    PvuI        PvuII       RcaI        
RsrII       SacI        SacII       SalI        SbfI        
SexAI       SfiI        SfoI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        Sse8387I    SspI        
StuI        SwaI        Tth111I     Van91I      XbaI        
XcmI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

ApaI          526  GGGCCC               GGGCC^C
AseI         1090  ATTAAT               AT^TAAT
AsnI         1090  ATTAAT               AT^TAAT
AvaI         1067  CTCGGG               C^[CT]CG[GA]G
AviII         221  TGCGCA               TGC^GCA
BanII         526  GGGCCC               G[GA]GC[CT]^C
BbsI          673  GTCTTC               GAAGAC(2/6)
BlpI          408  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      408  GCTGAGC              GC^T[ACGT]AGC
BpuAI         673  GTCTTC               GAAGAC(2/6)
BsaAI         838  CACGTA               [CT]AC^GT[GA]
BsgI          375  CTGCAC               GTGCAG(16/14)
BsoBI        1067  CTCGGG               C^[CT]CG[GA]G
Bsp1407I      965  TGTACA               T^GTACA
BsrFI         317  ACCGGC               [GA]^CCGG[CT]
BsrGI         965  TGTACA               T^GTACA
BstBI        1018  TTCGAA               TT^CGAA
BstEII        462  GGTCACC              G^GT[ACGT]ACC
BstPI         462  GGTCACC              G^GT[ACGT]ACC
CelII         408  GCTGAGC              GC^T[ACGT]AGC
Cfr10I        317  ACCGGC               [GA]^CCGG[CT]
EaeI           47  TGGCCG               [CT]^GGCC[GA]
EcoNI         752  CCTCGCTCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       462  GGTCACC              G^GT[ACGT]ACC
FspI          221  TGCGCA               TGC^GCA
MaeII         839  ACGT                 A^CGT
MseI         1091  TTAA                 T^TAA
MspA1I        121  CCGCTG               C[AC]G^C[GT]G
NspI          284  GCATGC               [GA]CATG^[CT]
NspV         1018  TTCGAA               TT^CGAA
PleI           82  GACTC                GAGTC(4/5)
PshBI        1090  ATTAAT               AT^TAAT
ScaI          905  AGTACT               AGT^ACT
SfuI         1018  TTCGAA               TT^CGAA
SphI          284  GCATGC               GCATG^C
SspBI         965  TGTACA               T^GTACA
Tru9I        1091  TTAA                 T^TAA
Tsp509I       359  AATT                 ^AATT


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AcyI           12  GACGCC               G[GA]^CG[CT]C
              133  GGCGTC              
Aor51HI       405  AGCGCT               AGC^GCT
              419  AGCGCT              
AspHI         391  GTGCTC               G[AT]GC[AT]^C
              553  GAGCAC              
BanI          565  GGCACC               G^G[CT][GA]CC
              748  GGTGCC              
BfaI          541  CTAG                 C^TAG
             1085  CTAG                
BsaHI          12  GACGCC               G[GA]^CG[CT]C
              133  GGCGTC              
BseRI         340  CTCCTC               GAGGAG(10/8)
              723  GAGGAG              
BsiHKAI       391  GTGCTC               G[AT]GC[AT]^C
              553  GAGCAC              
BsmAI         680  GTCTC                GTCTC(1/5)
              698  GTCTC               
BsmFI         578  GGGAC                GGGAC(10/14)
              761  GGGAC               
BspMI         145  ACCTGC               ACCTGC(4/8)
              182  ACCTGC              
BssSI         556  CACGAG               CACGAG(-5/-1)
              916  CTCGTG              
BstF5I        816  CATCC                GGATG(2/0)
               63  GGATG               
DpnII         265  GATC                 ^GATC
              897  GATC                
DsaI          386  CCACGG               C^C[GA][CT]GG
              506  CCGTGG              
Eco47III      405  AGCGCT               AGC^GCT
              419  AGCGCT              
FokI          816  CATCC                GGATG(9/13)
               63  GGATG               
Hin1I          12  GACGCC               G[GA]^CG[CT]C
              133  GGCGTC              
MaeI          541  CTAG                 C^TAG
             1085  CTAG                
MaeIII        136  GTCAC                ^GT[ACGT]AC
              463  GTCAC               
MboI          265  GATC                 ^GATC
              897  GATC                
NdeII         265  GATC                 ^GATC
              897  GATC                
PstI          103  CTGCAG               CTGCA^G
              516  CTGCAG              
SapI           22  GAAGAGC              GCTCTTC(1/4)
              108  GCTCTTC             
Sau3AI        265  GATC                 ^GATC
              897  GATC                
TaqI          416  TCGA                 T^CGA
             1019  TCGA                
TfiI         1016  GATTC                G^A[AT]TC
             1081  GAATC               
Tsp45I        136  GTCAC                ^GT[GC]AC
              463  GTCAC               
TthHB8I       416  TCGA                 T^CGA
             1019  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   55 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AvaII       
BbvI        BcnI        BmyI        BsaJI       BsiYI       
BslI        Bsp1286I    BsrI        BssKI       BstNI       
BstUI       BstXI       Cac8I       CfoI        Cfr13I      
DdeI        EarI        EcoRII      EcoT14I     FauI        
Fnu4HI      HaeII       HaeIII      HapII       HgaI        
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MboII       MnlI        MslI        
MspI        MvaI        MvnI        MwoI        NciI        
NlaIII      NlaIV       RsaI        Sau96I      ScrFI       
SfaNI       SfcI        StyI        TseI        TspRI       
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