Restriction map for pF1KE1096
 (Linear) (Six-base) MAPSORT of: KIEE1096.seq  Check: 8658  from: 1  to: 1098



KIEE1096.orf

 With 182 enzymes: SgfI * 

                        November 19, 2009 09:54  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     154    1098 
   Size:        154     944
 
AfeI AGC'GCT

Cuts at:      0     407     421    1098 
   Size:        407      14     677

  Fragments arranged by size: 

                 677     407      14
 
AleI CACnn'nnGTG

Cuts at:      0     148    1098 
   Size:        148     950
 
ApaI G_GGCC'C

Cuts at:      0     530    1098 
   Size:        530     568
 
AseI AT'TA_AT

Cuts at:      0    1091    1098 
   Size:       1091       7
 
AvaI C'yCGr_G

Cuts at:      0    1067    1098 
   Size:       1067      31
 
BanI G'GyrC_C

Cuts at:      0     565     748    1098 
   Size:        565     183     350

  Fragments arranged by size: 

                 565     350     183
 
BanII G_rGCy'C

Cuts at:      0     530    1098 
   Size:        530     568
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     666    1098 
   Size:        666     432
 
BlpI GC'TnA_GC

Cuts at:      0     409    1098 
   Size:        409     689
 
Bme1580I G_kGCm'C

Cuts at:      0     530    1098 
   Size:        530     568
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      69    1098 
   Size:         69    1029
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     894    1098 
   Size:        894     204
 
Bpu10I CC'TnA_GC

Cuts at:      0     757    1098 
   Size:        757     341
 
BsaAI yAC'GTr

Cuts at:      0     840    1098 
   Size:        840     258
 
BsaHI Gr'CG_yC

Cuts at:      0      13     134    1098 
   Size:         13     121     964

  Fragments arranged by size: 

                 964     121      13
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     331     738    1098 
   Size:        331     407     360

  Fragments arranged by size: 

                 407     360     331
 
BseYI C'CCAG_C

Cuts at:      0      16      87    1098 
   Size:         16      71    1011

  Fragments arranged by size: 

                1011      71      16
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     360    1098 
   Size:        360     738
 
BsiHKAI G_wGCw'C

Cuts at:      0     395     557    1098 
   Size:        395     162     541

  Fragments arranged by size: 

                 541     395     162
 
Bsp1286I G_dGCh'C

Cuts at:      0     395     530     557    1098 
   Size:        395     135      27     541

  Fragments arranged by size: 

                 541     395     135      27
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     154     191    1098 
   Size:        154      37     907

  Fragments arranged by size: 

                 907     154      37
 
BsrFI r'CCGG_y

Cuts at:      0     317    1098 
   Size:        317     781
 
BsrGI T'GTAC_A

Cuts at:      0     965    1098 
   Size:        965     133
 
BssSI C'ACGA_G

Cuts at:      0     556     916    1098 
   Size:        556     360     182

  Fragments arranged by size: 

                 556     360     182
 
BstBI TT'CG_AA

Cuts at:      0    1019    1098 
   Size:       1019      79
 
BstEII G'GTnAC_C

Cuts at:      0     462    1098 
   Size:        462     636
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     150     198     459    1098 
   Size:        150      48     261     639

  Fragments arranged by size: 

                 639     261     150      48
 
BtgI C'CryG_G

Cuts at:      0     386     506    1098 
   Size:        386     120     592

  Fragments arranged by size: 

                 592     386     120
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     156     749    1098 
   Size:        156     593     349

  Fragments arranged by size: 

                 593     349     156
 
BtsI GCAGTG_nn'

Cuts at:      0    1004    1098 
   Size:       1004      94
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     588     623    1098 
   Size:        588      35     475

  Fragments arranged by size: 

                 588     475      35
 
EaeI y'GGCC_r

Cuts at:      0      47    1098 
   Size:         47    1051
 
EarI CTCTTCn'nnn_

Cuts at:      0      17     115     163     358     937    1098 
   Size:         17      98      48     195     579     161

  Fragments arranged by size: 

                 579     195     161      98      48      17
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     735    1098 
   Size:        735     363
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     756    1098 
   Size:        756     342
 
FspI TGC'GCA

Cuts at:      0     223    1098 
   Size:        223     875
 
HaeII r_GCGC'y

Cuts at:      0     409     423     524    1098 
   Size:        409      14     101     574

  Fragments arranged by size: 

                 574     409     101      14
 
MslI CAynn'nnrTG

Cuts at:      0     148     196     535     634    1098 
   Size:        148      48     339      99     464

  Fragments arranged by size: 

                 464     339     148      99      48
 
MspA1I CmG'CkG

Cuts at:      0     123    1098 
   Size:        123     975
 
NspI r_CATG'y

Cuts at:      0     288    1098 
   Size:        288     810
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      16      48    1098 
   Size:         16      32    1050

  Fragments arranged by size: 

                1050      32      16
 
PspOMI G'GGCC_C

Cuts at:      0     526    1098 
   Size:        526     572
 
PstI C_TGCA'G

Cuts at:      0     107     520    1098 
   Size:        107     413     578

  Fragments arranged by size: 

                 578     413     107
 
SapI GCTCTTCn'nnn_

Cuts at:      0      17     115    1098 
   Size:         17      98     983

  Fragments arranged by size: 

                 983      98      17
 
ScaI AGT'ACT

Cuts at:      0     907    1098 
   Size:        907     191
 
SfcI C'TryA_G

Cuts at:      0     103     516     693     797    1098 
   Size:        103     413     177     104     301

  Fragments arranged by size: 

                 413     301     177     104     103
 
SphI G_CATG'C

Cuts at:      0     288    1098 
   Size:        288     810
 
StyI C'CwwG_G

Cuts at:      0     452     467     574     881    1098 
   Size:        452      15     107     307     217

  Fragments arranged by size: 

                 452     307     217     107      15
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     633     736    1098 
   Size:        633     103     362

  Fragments arranged by size: 

                 633     362     103
 
TatI w'GTAC_w

Cuts at:      0     905     965    1098 
   Size:        905      60     133

  Fragments arranged by size: 

                 905     133      60
 

 Enzymes that do cut:

     AarI     AfeI     AleI     ApaI     AseI     AvaI     BanI    BanII
     BbsI     BlpI Bme1580I     BmrI     BplI   Bpu10I    BsaAI    BsaHI
    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BspMI    BsrFI    BsrGI
    BssSI    BstBI   BstEII    BstXI     BtgI    BtgZI     BtsI    CspCI
     EaeI     EarI     EciI    EcoNI     FspI    HaeII     MslI   MspA1I
     NspI     PpiI   PspOMI     PstI     SapI     ScaI     SfcI     SphI
     StyI    TaqII     TatI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AlfI     AloI    AlwNI    ApaLI     ApoI     AscI    AsiSI
    AvrII     BaeI    BamHI     BbeI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII    BmgBI     BmtI     BpmI    BpuEI     BsaI
    BsaBI    BsaWI    BsaXI    BsiEI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BsrBI    BsrDI   BssHII   BstAPI    BstYI  BstZ17I   Bsu36I
     ClaI     DraI   DraIII     DrdI     EagI  EcoICRI  Eco57MI EcoO109I
    EcoRI    EcoRV     FalI     FseI    FspAI    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI    PpuMI
    PshAI     PsiI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SbfI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SrfI     SspI     StuI
     SwaI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI
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