Restriction map for
pF1KB7687
(Circular) (Six-base) MAPSORT of: pF1KB7687.seq Check: 3706 from: 1 to: 4157
pF1KB7687 4157 bp
With 182 enzymes: SgfI *
October 19, 2009 13:28 ..
AccI GT'mk_AC
Cuts at: 1195 1195
Size: 4157
Acc65I G'GTAC_C
Cuts at: 1173 1173
Size: 4157
AclI AA'CG_TT
Cuts at: 3997 3997
Size: 4157
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 217 724 1982 2414 3161 3429 217
Size: 507 1258 432 747 268 945
Fragments arranged by size:
1258 945 747 507 432 268
AflIII A'CryG_T
Cuts at: 1509 2613 3590 1509
Size: 1104 977 2076
Fragments arranged by size:
2076 1104 977
AgeI A'CCGG_T
Cuts at: 2545 2545
Size: 4157
AhdI GACnn_n'nnGTC
Cuts at: 507 507
Size: 4157
AlwNI CAG_nnn'CTG
Cuts at: 1462 3029 1462
Size: 1567 2590
ApaI G_GGCC'C
Cuts at: 186 186
Size: 4157
ApaLI G'TGCA_C
Cuts at: 2927 2927
Size: 4157
ApoI r'AATT_y
Cuts at: 1161 3510 3766 1161
Size: 2349 256 1552
Fragments arranged by size:
2349 1552 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4157
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4157
AvaI C'yCGr_G
Cuts at: 1177 1177
Size: 4157
BamHI G'GATC_C
Cuts at: 1182 1182
Size: 4157
BanI G'GyrC_C
Cuts at: 1012 1173 1587 1835 1870 1012
Size: 161 414 248 35 3299
Fragments arranged by size:
3299 414 248 161 35
BanII G_rGCy'C
Cuts at: 186 583 788 1100 1171 2201 186
Size: 397 205 312 71 1030 2142
Fragments arranged by size:
2142 1030 397 312 205 71
BbeI G_GCGC'C
Cuts at: 1591 1839 1591
Size: 248 3909
BbsI GAAGACnn'nnnn_
Cuts at: 479 503 479
Size: 24 4133
BbvCI CC'TCA_GC
Cuts at: 130 130
Size: 4157
BciVI GTATCCnnnnn_n'
Cuts at: 2055 2816 4157 2055
Size: 761 1341 2055
Fragments arranged by size:
2055 1341 761
BclI T'GATC_A
Cuts at: 1677 1677
Size: 4157
BglI GCCn_nnn'nGGC
Cuts at: 706 706
Size: 4157
BglII A'GATC_T
Cuts at: 1672 3709 1672
Size: 2037 2120
BlpI GC'TnA_GC
Cuts at: 1269 1269
Size: 4157
Bme1580I G_kGCm'C
Cuts at: 186 1782 1875 2931 186
Size: 1596 93 1056 1412
Fragments arranged by size:
1596 1412 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1369 1450 1785 3929 1369
Size: 81 335 2144 1597
Fragments arranged by size:
2144 1597 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2524 2524
Size: 4157
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 169 169
Size: 4157
Bpu10I CC'TnA_GC
Cuts at: 130 130
Size: 4157
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 436 772 826 1333 2212 2704 3002 436
Size: 336 54 507 879 492 298 1591
Fragments arranged by size:
1591 879 507 492 336 298 54
BsaI GGTCTCn'nnnn_
Cuts at: 3861 3861
Size: 4157
BsaAI yAC'GTr
Cuts at: 1510 1510
Size: 4157
BsaBI GATnn'nnATC
Cuts at: 6 223 1671 3708 6
Size: 217 1448 2037 455
Fragments arranged by size:
2037 1448 455 217
BsaHI Gr'CG_yC
Cuts at: 1588 1836 2538 4034 1588
Size: 248 702 1496 1711
Fragments arranged by size:
1711 1496 702 248
BsaWI w'CCGG_w
Cuts at: 809 1346 1867 2545 2819 2966 3377 809
Size: 537 521 678 274 147 411 1589
Fragments arranged by size:
1589 678 537 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 245 275 286 316 676 706 245
Size: 30 11 30 360 30 3696
Fragments arranged by size:
3696 360 30 30 30 11
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 136 510 513 136
Size: 374 3 3780
Fragments arranged by size:
3780 374 3
BseYI C'CCAG_C
Cuts at: 1582 2340 2917 3413 1582
Size: 758 577 496 2326
Fragments arranged by size:
2326 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 190 1044 1056 1096 190
Size: 854 12 40 3251
Fragments arranged by size:
3251 854 40 12
BsiEI CG_ry'CG
Cuts at: 104 1699 1745 2953 104
Size: 1595 46 1208 1308
Fragments arranged by size:
1595 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 788 796 881 992 1171 1949 2139 2931 788
Size: 8 85 111 179 778 190 792 2014
Fragments arranged by size:
2014 792 778 190 179 111 85 8
BsmI GAATG_Cn'
Cuts at: 966 966
Size: 4157
Bsp1286I G_dGCh'C
Cuts at: 186 583 788 796 881 992 1100 1171 1782
Size: 397 205 8 85 111 108 71 611
Cuts at: 1782 1875 1949 2139 2201 2931 186
Size: 93 74 190 62 730 1412
Fragments arranged by size:
1412 730 611 397 205 190 111 108
93 85 74 71 62 8
BspEI T'CCGG_A
Cuts at: 3377 3377
Size: 4157
BspHI T'CATG_A
Cuts at: 3333 3333
Size: 4157
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1207 1421 1723 2104 1207
Size: 214 302 381 3260
Fragments arranged by size:
3260 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2449 2503 3987 4152 50
Size: 2399 54 1484 165 55
Fragments arranged by size:
2399 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1128 2069 1128
Size: 941 3216
BsrFI r'CCGG_y
Cuts at: 2155 2336 2545 2155
Size: 181 209 3767
Fragments arranged by size:
3767 209 181
BssSI C'ACGA_G
Cuts at: 2428 2786 2428
Size: 358 3799
BstAPI GCAn_nnn'nTGC
Cuts at: 281 1043 1534 281
Size: 762 491 2904
Fragments arranged by size:
2904 762 491
BstBI TT'CG_AA
Cuts at: 2518 2518
Size: 4157
BstXI CCAn_nnnn'nTGG
Cuts at: 147 147
Size: 4157
BstYI r'GATC_y
Cuts at: 1 1182 1649 1672 2007 2253 3265 3351 3363
Size: 1181 467 23 335 246 1012 86 12
Cuts at: 3363 3709 1
Size: 346 449
Fragments arranged by size:
1181 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4157
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2107 2288 2451 2107
Size: 181 163 3813
Fragments arranged by size:
3813 181 163
BtsI GCAGTG_nn'
Cuts at: 279 1092 1400 279
Size: 813 308 3036
Fragments arranged by size:
3036 813 308
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1110 1145 1110
Size: 35 4122
DraI TTT'AAA
Cuts at: 1156 1156
Size: 4157
DraIII CAC_nnn'GTG
Cuts at: 1087 1087
Size: 4157
DrdI GACnn_nn'nnGTC
Cuts at: 733 1863 2721 733
Size: 1130 858 2169
Fragments arranged by size:
2169 1130 858
EaeI y'GGCC_r
Cuts at: 161 308 932 998 1742 1916 2307 2334 3679
Size: 147 624 66 744 174 391 27 1345
Cuts at: 3679 161
Size: 639
Fragments arranged by size:
1345 744 639 624 391 174 147 66
27
EagI C'GGCC_G
Cuts at: 1742 1742
Size: 4157
EarI CTCTTCn'nnn_
Cuts at: 113 627 1033 2180 2390 113
Size: 514 406 1147 210 1880
Fragments arranged by size:
1880 1147 514 406 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 724 885 2365 2675 2821 3967 724
Size: 161 1480 310 146 1146 914
Fragments arranged by size:
1480 1146 914 310 161 146
EcoICRI GAG'CTC
Cuts at: 786 1169 786
Size: 383 3774
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 169 217 724 1982 2414 3161 3429 169
Size: 48 507 1258 432 747 268 897
Fragments arranged by size:
1258 897 747 507 432 268 48
EcoO109I rG'GnC_Cy
Cuts at: 182 183 846 1296 182
Size: 1 663 450 3043
Fragments arranged by size:
3043 663 450 1
EcoRI G'AATT_C
Cuts at: 1161 1161
Size: 4157
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 463 2176 463
Size: 1713 2444
FspI TGC'GCA
Cuts at: 823 1938 823
Size: 1115 3042
HaeII r_GCGC'y
Cuts at: 1591 1839 2861 3840 1591
Size: 248 1022 979 1908
Fragments arranged by size:
1908 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 286 318 493 525 676 708 1999 2029 2031
Size: 32 175 32 151 32 1291 30 2
Cuts at: 2031 2061 286
Size: 30 2382
Fragments arranged by size:
2382 1291 175 151 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 738 1196 738
Size: 458 3699
KasI G'GCGC_C
Cuts at: 1587 1835 1587
Size: 248 3909
KpnI G_GTAC'C
Cuts at: 1177 1177
Size: 4157
MluI A'CGCG_T
Cuts at: 3590 3590
Size: 4157
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2828 3012 2828
Size: 184 3973
MscI TGG'CCA
Cuts at: 163 310 934 1000 1918 163
Size: 147 624 66 918 2402
Fragments arranged by size:
2402 918 624 147 66
MslI CAynn'nnrTG
Cuts at: 799 2146 2273 799
Size: 1347 127 2683
Fragments arranged by size:
2683 1347 127
MspA1I CmG'CkG
Cuts at: 212 1268 1582 1942 2955 3200 212
Size: 1056 314 360 1013 245 1169
Fragments arranged by size:
1169 1056 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2338 2338
Size: 4157
NarI GG'CG_CC
Cuts at: 1588 1836 1588
Size: 248 3909
NcoI C'CATG_G
Cuts at: 107 107
Size: 4157
NgoMIV G'CCGG_C
Cuts at: 2336 2336
Size: 4157
NspI r_CATG'y
Cuts at: 1210 1710 2617 1210
Size: 500 907 2750
Fragments arranged by size:
2750 907 500
PciI A'CATG_T
Cuts at: 2613 2613
Size: 4157
PfoI T'CCnGG_A
Cuts at: 668 668
Size: 4157
PmeI GTTT'AAAC
Cuts at: 1156 1156
Size: 4157
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3296 3328 3296
Size: 32 4125
PpuMI rG'GwC_Cy
Cuts at: 846 846
Size: 4157
PspOMI G'GGCC_C
Cuts at: 182 182
Size: 4157
PstI C_TGCA'G
Cuts at: 1204 1889 1204
Size: 685 3472
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4157
PvuII CAG'CTG
Cuts at: 212 1582 1942 212
Size: 1370 360 2427
Fragments arranged by size:
2427 1370 360
RsrII CG'GwC_CG
Cuts at: 2352 2352
Size: 4157
SacI G_AGCT'C
Cuts at: 788 1171 788
Size: 383 3774
SalI G'TCGA_C
Cuts at: 1194 1194
Size: 4157
SapI GCTCTTCn'nnn_
Cuts at: 2180 2390 2180
Size: 210 3947
SbfI CC_TGCA'GG
Cuts at: 1204 1204
Size: 4157
ScaI AGT'ACT
Cuts at: 3674 3674
Size: 4157
SexAI A'CCwGG_T
Cuts at: 1070 1070
Size: 4157
SfcI C'TryA_G
Cuts at: 33 235 1200 1885 2878 3069 33
Size: 202 965 685 993 191 1121
Fragments arranged by size:
1121 993 965 685 202 191
SfoI GGC'GCC
Cuts at: 1589 1837 1589
Size: 248 3909
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4157
SmaI CCC'GGG
Cuts at: 1179 1179
Size: 4157
SmlI C'TyrA_G
Cuts at: 415 787 841 1312 2227 2719 2981 415
Size: 372 54 471 915 492 262 1591
Fragments arranged by size:
1591 915 492 471 372 262 54
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4157
SphI G_CATG'C
Cuts at: 1210 1710 1210
Size: 500 3657
StuI AGG'CCT
Cuts at: 446 446
Size: 4157
StyI C'CwwG_G
Cuts at: 107 305 457 1291 1645 107
Size: 198 152 834 354 2619
Fragments arranged by size:
2619 834 354 198 152
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1740 1873 2332 2541 1740
Size: 133 459 209 3356
Fragments arranged by size:
3356 459 209 133
TatI w'GTAC_w
Cuts at: 3672 3672
Size: 4157
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3674 3706 3674
Size: 32 4125
Tth111I GACn'n_nGTC
Cuts at: 664 1954 664
Size: 1290 2867
XbaI T'CTAG_A
Cuts at: 1188 1188
Size: 4157
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 633 1652 633
Size: 1019 3138
XmaI C'CCGG_G
Cuts at: 1177 1177
Size: 4157
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PfoI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI AscI
AvrII BaeI BcgI BfrBI BmgBI BmtI BsiWI BsmBI
BsrGI BssHII BstEII BstZ17I Bsu36I ClaI EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PmlI PshAI PsiI
PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI XmnI ZraI