Restriction map for
pF1KB7687
(Linear) (Six-base) MAPSORT of: KIBB7687.seq Check: 4483 from: 1 to: 1044
KIBB7687.orf
With 182 enzymes: SgfI *
October 19, 2009 13:28 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 616 1044
Size: 109 507 428
Fragments arranged by size:
507 428 109
AhdI GACnn_n'nnGTC
Cuts at: 0 399 1044
Size: 399 645
ApaI G_GGCC'C
Cuts at: 0 78 1044
Size: 78 966
BanI G'GyrC_C
Cuts at: 0 904 1044
Size: 904 140
BanII G_rGCy'C
Cuts at: 0 78 475 680 992 1044
Size: 78 397 205 312 52
Fragments arranged by size:
397 312 205 78 52
BbsI GAAGACnn'nnnn_
Cuts at: 0 371 395 1044
Size: 371 24 649
Fragments arranged by size:
649 371 24
BbvCI CC'TCA_GC
Cuts at: 0 22 1044
Size: 22 1022
BglI GCCn_nnn'nGGC
Cuts at: 0 598 1044
Size: 598 446
Bme1580I G_kGCm'C
Cuts at: 0 78 1044
Size: 78 966
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 1044
Size: 61 983
Bpu10I CC'TnA_GC
Cuts at: 0 22 1044
Size: 22 1022
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 328 664 718 1044
Size: 328 336 54 326
Fragments arranged by size:
336 328 326 54
BsaBI GATnn'nnATC
Cuts at: 0 115 1044
Size: 115 929
BsaWI w'CCGG_w
Cuts at: 0 701 1044
Size: 701 343
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 137 167 178 208 568 598 1044
Size: 137 30 11 30 360 30 446
Fragments arranged by size:
446 360 137 30 30 30 11
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 28 402 405 1044
Size: 28 374 3 639
Fragments arranged by size:
639 374 28 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 936 948 988 1044
Size: 82 854 12 40 56
Fragments arranged by size:
854 82 56 40 12
BsiHKAI G_wGCw'C
Cuts at: 0 680 688 773 884 1044
Size: 680 8 85 111 160
Fragments arranged by size:
680 160 111 85 8
BsmI GAATG_Cn'
Cuts at: 0 858 1044
Size: 858 186
Bsp1286I G_dGCh'C
Cuts at: 0 78 475 680 688 773 884 992 1044
Size: 78 397 205 8 85 111 108 52
Fragments arranged by size:
397 205 111 108 85 78 52 8
BsrDI GCAATG_nn'
Cuts at: 0 1020 1044
Size: 1020 24
BstAPI GCAn_nnn'nTGC
Cuts at: 0 173 935 1044
Size: 173 762 109
Fragments arranged by size:
762 173 109
BstXI CCAn_nnnn'nTGG
Cuts at: 0 39 1044
Size: 39 1005
BtsI GCAGTG_nn'
Cuts at: 0 171 984 1044
Size: 171 813 60
Fragments arranged by size:
813 171 60
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1002 1037 1044
Size: 1002 35 7
Fragments arranged by size:
1002 35 7
DraIII CAC_nnn'GTG
Cuts at: 0 979 1044
Size: 979 65
DrdI GACnn_nn'nnGTC
Cuts at: 0 625 1044
Size: 625 419
EaeI y'GGCC_r
Cuts at: 0 53 200 824 890 1044
Size: 53 147 624 66 154
Fragments arranged by size:
624 154 147 66 53
EarI CTCTTCn'nnn_
Cuts at: 0 5 519 925 1044
Size: 5 514 406 119
Fragments arranged by size:
514 406 119 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 616 777 1044
Size: 616 161 267
Fragments arranged by size:
616 267 161
EcoICRI GAG'CTC
Cuts at: 0 678 1044
Size: 678 366
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 109 616 1044
Size: 61 48 507 428
Fragments arranged by size:
507 428 61 48
EcoO109I rG'GnC_Cy
Cuts at: 0 74 75 738 1044
Size: 74 1 663 306
Fragments arranged by size:
663 306 74 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 355 1044
Size: 355 689
FspI TGC'GCA
Cuts at: 0 715 1044
Size: 715 329
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 178 210 385 417 568 600 1044
Size: 178 32 175 32 151 32 444
Fragments arranged by size:
444 178 175 151 32 32 32
HincII GTy'rAC
Cuts at: 0 630 1044
Size: 630 414
MscI TGG'CCA
Cuts at: 0 55 202 826 892 1044
Size: 55 147 624 66 152
Fragments arranged by size:
624 152 147 66 55
MslI CAynn'nnrTG
Cuts at: 0 691 1044
Size: 691 353
MspA1I CmG'CkG
Cuts at: 0 104 1044
Size: 104 940
PfoI T'CCnGG_A
Cuts at: 0 560 1044
Size: 560 484
PpuMI rG'GwC_Cy
Cuts at: 0 738 1044
Size: 738 306
PspOMI G'GGCC_C
Cuts at: 0 74 1044
Size: 74 970
PvuII CAG'CTG
Cuts at: 0 104 1044
Size: 104 940
SacI G_AGCT'C
Cuts at: 0 680 1044
Size: 680 364
SexAI A'CCwGG_T
Cuts at: 0 962 1044
Size: 962 82
SfcI C'TryA_G
Cuts at: 0 127 1044
Size: 127 917
SmlI C'TyrA_G
Cuts at: 0 307 679 733 1044
Size: 307 372 54 311
Fragments arranged by size:
372 311 307 54
StuI AGG'CCT
Cuts at: 0 338 1044
Size: 338 706
StyI C'CwwG_G
Cuts at: 0 197 349 1044
Size: 197 152 695
Fragments arranged by size:
695 197 152
Tth111I GACn'n_nGTC
Cuts at: 0 556 1044
Size: 556 488
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 525 1044
Size: 525 519
Enzymes that do cut:
AcuI AhdI ApaI BanI BanII BbsI BbvCI BglI
Bme1580I BpmI Bpu10I BpuEI BsaBI BsaWI BsaXI BseRI
BsgI BsiHKAI BsmI Bsp1286I BsrDI BstAPI BstXI BtsI
CspCI DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI
EcoO109I FalI FspI Hin4I HincII MscI MslI MspA1I
PfoI PpuMI PspOMI PvuII SacI SexAI SfcI SmlI
StuI StyI Tth111I XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AleI AlfI AloI AlwNI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BbeI BcgI
BciVI BclI BfrBI BglII BlpI BmgBI BmrI BmtI
BplI BsaI BsaAI BsaHI BseYI BsiEI BsiWI BsmBI
BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI
BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI ClaI
DraI EagI EcoNI EcoRI EcoRV FseI FspAI HaeII
HindIII HpaI KasI KpnI MfeI MluI MmeI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PasI PciI PflMI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PstI PvuI RsrII SacII
SalI SanDI SapI SbfI ScaI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI
TaqII TatI TstI XbaI XhoI XmaI XmnI ZraI