Restriction map for pF1KB6181
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# NO CUT ENZYMES (  121 enzymes) #
##################################

Acc65I      AccIII      AclI        AflII       AflIII      
AgeI        AhdI        Alw44I      AocI        Aor51HI     
ApaLI       AscI        Asp718I     AspEI       AvaI        
AviII       AvrII       BalI        BamHI       BbrPI       
BbvCI       BcgI        BciVI       BcnI        BfrI        
BglI        BglII       BlnI        BlpI        Bpu1102I    
BsaAI       BsaWI       BseAI       BsiEI       BsiWI       
BsmBI       BsoBI       Bsp1407I    BspDI       BspEI       
BspLU11I    BsrBI       BsrDI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstXI       BstYI       
BstZ17I     Bsu36I      CelII       ClaI        CpoI        
DraIII      DrdI        DsaI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoRV       
EcoT22I     FauI        FseI        FspI        HgaI        
KpnI        KspI        MflI        MluI        MluNI       
MroI        MscI        NaeI        NciI        NcoI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        PacI        PaeR7I      PflMI       PinAI       
PleI        PmaCI       PmeI        PmlI        PpuMI       
PshAI       Psp1406I    PstI        PvuI        RsrII       
SacII       SapI        SbfI        SfcI        SfiI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        Tsp45I      
Van91I      XcmI        XhoI        XhoII       XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   66 enzymes) #
###################################

AatII        1484  GACGTC               GACGT^C
AccI         1807  GTCGAC               GT^[AC][GT]AC
AlwI         1365  GATCC                GGATC(4/5)
AlwNI         205  CAGATTCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI          684  GGGCCC               GGGCC^C
AseI         1179  ATTAAT               AT^TAAT
AsnI         1179  ATTAAT               AT^TAAT
AspHI         677  GAGCTC               G[AT]GC[AT]^C
AspI         1639  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbeI           86  GGCGCC               GGCGC^C
BclI         1326  TGATCA               T^GATCA
BpmI         1289  CTGGAG               CTGGAG(16/14)
BsaBI        1198  GATTTAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI       677  GAGCTC               G[AT]GC[AT]^C
BspHI        1896  TCATGA               T^CATGA
BspMI        1907  ACCTGC               ACCTGC(4/8)
BsrFI        1153  GCCGGT               [GA]^CCGG[CT]
BssKI        1282  CCTGG                ^CC[ACGT]GG
BstBI         740  TTCGAA               TT^CGAA
BstEII        985  GGTTACC              G^GT[ACGT]ACC
BstNI        1282  CCTGG                CC^[AT]GG
BstPI         985  GGTTACC              G^GT[ACGT]ACC
Cfr10I       1153  GCCGGT               [GA]^CCGG[CT]
DraI         1200  TTTAAA               TTT^AAA
DraII         683  AGGGCCC              [GA]G^G[ACGT]CC[CT]
EaeI         1151  TGGCCG               [CT]^GGCC[GA]
EarI         1858  CTCTTC               CTCTTC(1/4)
EcoO109I      683  AGGGCCC              [GA]G^G[ACGT]CC[CT]
EcoO65I       985  GGTTACC              G^GT[ACGT]ACC
EcoRI        1936  GAATTC               G^AATTC
EcoRII       1282  CCTGG                ^CC[AT]GG
EcoT14I      1545  CCTTGG               C^C[AT][AT]GG
FbaI         1326  TGATCA               T^GATCA
HaeII          86  GGCGCC               [GA]GCGC^[CT]
HapII        1154  CCGG                 C^CGG
HindIII       503  AAGCTT               A^AGCTT
HpaI          660  GTTAAC               GTT^AAC
HpaII        1154  CCGG                 C^CGG
HphI         1459  GGTGA                GGTGA(8/7)
KasI           86  GGCGCC               G^GCGCC
Ksp632I      1858  CTCTTC               CTCTTC(1/4)
MamI         1198  GATTTAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI          671  CAATTG               C^AATTG
MslI         1912  CATATGGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        559  CAGCTG               C[AC]G^C[GT]G
MspI         1154  CCGG                 C^CGG
MunI          671  CAATTG               C^AATTG
MvaI         1282  CCTGG                CC^[AT]GG
NarI           86  GGCGCC               GG^CGCC
NdeI         1912  CATATG               CA^TATG
NspV          740  TTCGAA               TT^CGAA
PflFI        1639  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshBI        1179  ATTAAT               AT^TAAT
PvuII         559  CAGCTG               CAG^CTG
RcaI         1896  TCATGA               T^CATGA
SacI          677  GAGCTC               GAGCT^C
SalI         1807  GTCGAC               G^TCGAC
ScrFI        1282  CCTGG                CC^[ACGT]GG
SexAI        1281  ACCTGGT              A^CC[AT]GGT
SfaNI        1850  GCATC                GCATC(5/9)
SfoI           86  GGCGCC               GGC^GCC
SfuI          740  TTCGAA               TT^CGAA
StyI         1545  CCTTGG               C^C[AT][AT]GG
SwaI         1199  ATTTAAAT             ATTT^AAAT
Tth111I      1639  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI         1059  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AcyI           86  GGCGCC               G[GA]^CG[CT]C
             1484  GACGTC              
Asp700I       357  GAAAGATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1433  GAAAGAGTTC          
AvaII         222  GGTCC                G^G[AT]CC
             1417  GGTCC               
BanI           49  GGTGCC               G^G[CT][GA]CC
               86  GGCGCC              
BanII         677  GAGCTC               G[GA]GC[CT]^C
              684  GGGCCC              
BbsI          743  GAAGAC               GAAGAC(2/6)
              883  GTCTTC              
BmyI          677  GAGCTC               G[AGT]GC[ACT]^C
              684  GGGCCC              
BpuAI         743  GAAGAC               GAAGAC(2/6)
              883  GTCTTC              
BsaHI          86  GGCGCC               G[GA]^CG[CT]C
             1484  GACGTC              
BsaJI          96  CCTCGG               C^C[ACGT][ACGT]GG
             1545  CCTTGG              
BseRI          36  CTCCTC               GAGGAG(10/8)
              490  GAGGAG              
BsgI          103  CTGCAC               GTGCAG(16/14)
             1796  GTGCAG              
BsmI         1085  GAATGC               GAATGC(1/-1)
             1786  GAATGC              
Bsp1286I      677  GAGCTC               G[AGT]GC[ACT]^C
              684  GGGCCC              
EcoNI         483  CCTCTATGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1498  CCTGAAAGAGG         
Hin1I          86  GGCGCC               G[GA]^CG[CT]C
             1484  GACGTC              
HincII        660  GTTAAC               GT[CT]^[GA]AC
             1807  GTCGAC              
HindII        660  GTTAAC               GT[CT]^[GA]AC
             1807  GTCGAC              
XmnI          357  GAAAGATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1433  GAAAGAGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   52 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
ApoI        BbvI        BfaI        BsaI        BsiYI       
BslI        BsmAI       BsmFI       BsrI        BstF5I      
BstUI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       Fnu4HI      FokI        HaeIII      HhaI        
HinP1I      HinfI       ItaI        MaeI        MaeII       
MaeIII      MboI        MboII       MnlI        MseI        
MvnI        MwoI        NdeII       NlaIII      NlaIV       
RsaI        Sau3AI      Sau96I      ScaI        SmlI        
TaqI        TfiI        Tru9I       TseI        Tsp509I     
TspRI       TthHB8I     
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