Restriction map for pF1KB9655
 (Linear) (Six-base) MAPSORT of: KIBB9655.seq  Check: 1117  from: 1  to: 2046



KIBB9655.orf

 With 182 enzymes: SgfI * 

                        July 28, 2009 13:24  ..

AatII G_ACGT'C

Cuts at:      0     233    2046 
   Size:        233    1813
 
AfeI AGC'GCT

Cuts at:      0    1777    2046 
   Size:       1777     269
 
AleI CACnn'nnGTG

Cuts at:      0     838    1936    2046 
   Size:        838    1098     110

  Fragments arranged by size: 

                1098     838     110
 
ApaI G_GGCC'C

Cuts at:      0    1269    1357    2046 
   Size:       1269      88     689

  Fragments arranged by size: 

                1269     689      88
 
ApoI r'AATT_y

Cuts at:      0      46     646    2046 
   Size:         46     600    1400

  Fragments arranged by size: 

                1400     600      46
 
AscI GG'CGCG_CC

Cuts at:      0    1430    2046 
   Size:       1430     616
 
AvaI C'yCGr_G

Cuts at:      0    1261    1338    1357    1425    1884    2046 
   Size:       1261      77      19      68     459     162

  Fragments arranged by size: 

                1261     459     162      77      68      19
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     601     634    2046 
   Size:        601      33    1412

  Fragments arranged by size: 

                1412     601      33
 
BamHI G'GATC_C

Cuts at:      0      85     777    2046 
   Size:         85     692    1269

  Fragments arranged by size: 

                1269     692      85
 
BanI G'GyrC_C

Cuts at:      0     597     656    1636    1663    1729    1795    2046 
   Size:        597      59     980      27      66      66     251

  Fragments arranged by size: 

                 980     597     251      66      66      59      27
 
BanII G_rGCy'C

Cuts at:      0     561     891    1269    1357    1678    2046 
   Size:        561     330     378      88     321     368

  Fragments arranged by size: 

                 561     378     368     330     321      88
 
BbeI G_GCGC'C

Cuts at:      0    1640    1733    1799    2046 
   Size:       1640      93      66     247

  Fragments arranged by size: 

                1640     247      93      66
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     280     314     832     866    2046 
   Size:        280      34     518      34    1180

  Fragments arranged by size: 

                1180     518     280      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     496    2046 
   Size:        496    1550
 
BclI T'GATC_A

Cuts at:      0     671    2046 
   Size:        671    1375
 
BfrBI ATG'CAT

Cuts at:      0     864    2046 
   Size:        864    1182
 
BglI GCCn_nnn'nGGC

Cuts at:      0     639    1058    1607    1692    2046 
   Size:        639     419     549      85     354

  Fragments arranged by size: 

                 639     549     419     354      85
 
Bme1580I G_kGCm'C

Cuts at:      0     602    1262    1269    1357    2046 
   Size:        602     660       7      88     689

  Fragments arranged by size: 

                 689     660     602      88       7
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     353     893    2046 
   Size:        353     540    1153

  Fragments arranged by size: 

                1153     540     353
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1869    2046 
   Size:       1869     177
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      28     157     810    1860    2046 
   Size:         28     129     653    1050     186

  Fragments arranged by size: 

                1050     653     186     129      28
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1945    2046 
   Size:       1945     101
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     273     733    2046 
   Size:        273     460    1313

  Fragments arranged by size: 

                1313     460     273
 
BsaBI GATnn'nnATC

Cuts at:      0     673     782    2046 
   Size:        673     109    1264

  Fragments arranged by size: 

                1264     673     109
 
BsaHI Gr'CG_yC

Cuts at:      0     230    1459    1637    1730    1796    1808    1849    2046 
   Size:        230    1229     178      93      66      12      41     197

  Fragments arranged by size: 

                1229     230     197     178      93      66      41      12
            
 
BsaWI w'CCGG_w

Cuts at:      0     867    2046 
   Size:        867    1179
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     529     559     589    2046 
   Size:        529      30      30    1457

  Fragments arranged by size: 

                1457     529      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     307     480     567    1765    1845    2046 
   Size:        307     173      87    1198      80     201

  Fragments arranged by size: 

                1198     307     201     173      87      80
 
BseYI C'CCAG_C

Cuts at:      0     584    1631    1979    2046 
   Size:        584    1047     348      67

  Fragments arranged by size: 

                1047     584     348      67
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1087    1575    2046 
   Size:       1087     488     471

  Fragments arranged by size: 

                1087     488     471
 
BsiEI CG_ry'CG

Cuts at:      0     515    1062    1712    1928    2046 
   Size:        515     547     650     216     118

  Fragments arranged by size: 

                 650     547     515     216     118
 
BsiHKAI G_wGCw'C

Cuts at:      0      14     561    2046 
   Size:         14     547    1485

  Fragments arranged by size: 

                1485     547      14
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1840    2046 
   Size:       1840     206
 
Bsp1286I G_dGCh'C

Cuts at:      0      14     561     602     891    1262    1269    1357    1678 
   Size:         14     547      41     289     371       7      88     321

Cuts at:   1678    2046 
   Size:        368

  Fragments arranged by size: 

                 547     371     368     321     289      88      41      14
                   7
 
BspEI T'CCGG_A

Cuts at:      0     867    2046 
   Size:        867    1179
 
BsrBI CCG'CTC

Cuts at:      0    1605    1959    2046 
   Size:       1605     354      87

  Fragments arranged by size: 

                1605     354      87
 
BsrFI r'CCGG_y

Cuts at:      0     393     514     653    1198    1282    1461    1472    1726 
   Size:        393     121     139     545      84     179      11     254

Cuts at:   1726    1923    2046 
   Size:        197     123

  Fragments arranged by size: 

                 545     393     254     197     179     139     123     121
                  84      11
 
BssHII G'CGCG_C

Cuts at:      0    1430    1717    2046 
   Size:       1430     287     329

  Fragments arranged by size: 

                1430     329     287
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1257    2046 
   Size:       1257     789
 
BstYI r'GATC_y

Cuts at:      0      85     777    1916    2046 
   Size:         85     692    1139     130

  Fragments arranged by size: 

                1139     692     130      85
 
BtgI C'CryG_G

Cuts at:      0     509     737    1277    1508    1706    2046 
   Size:        509     228     540     231     198     340

  Fragments arranged by size: 

                 540     509     340     231     228     198
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1083    2046 
   Size:       1083     963
 
BtsI GCAGTG_nn'

Cuts at:      0      12     350    2046 
   Size:         12     338    1696

  Fragments arranged by size: 

                1696     338      12
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     183    2046 
   Size:        183    1863
 
EaeI y'GGCC_r

Cuts at:      0     347     381     512     540    1059    1280    1463    1505 
   Size:        347      34     131      28     519     221     183      42

Cuts at:   1505    1529    1596    1709    1724    1766    1925    2046 
   Size:         24      67     113      15      42     159     121

  Fragments arranged by size: 

                 519     347     221     183     159     131     121     113
                  67      42      42      34      28      24      15
 
EagI C'GGCC_G

Cuts at:      0     512    1059    1709    1925    2046 
   Size:        512     547     650     216     121

  Fragments arranged by size: 

                 650     547     512     216     121
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      98     790    1929    2046 
   Size:         98     692    1139     117

  Fragments arranged by size: 

                1139     692     117      98
 
EcoICRI GAG'CTC

Cuts at:      0     559    2046 
   Size:        559    1487
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      28     157     810    1860    2046 
   Size:         28     129     653    1050     186

  Fragments arranged by size: 

                1050     653     186     129      28
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1747    2046 
   Size:       1747     299
 
EcoO109I rG'GnC_Cy

Cuts at:      0     632    1265    1354    1422    1761    1821    2046 
   Size:        632     633      89      68     339      60     225

  Fragments arranged by size: 

                 633     632     339     225      89      68      60
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     167    1347    2046 
   Size:        167    1180     699

  Fragments arranged by size: 

                1180     699     167
 
HaeII r_GCGC'y

Cuts at:      0    1570    1640    1706    1733    1779    1799    2046 
   Size:       1570      70      66      27      46      20     247

  Fragments arranged by size: 

                1570     247      70      66      46      27      20
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     165     197     253     285    1936    1968    2046 
   Size:        165      32      56      32    1651      32      78

  Fragments arranged by size: 

                1651     165      78      56      32      32      32
 
KasI G'GCGC_C

Cuts at:      0    1636    1729    1795    2046 
   Size:       1636      93      66     251

  Fragments arranged by size: 

                1636     251      93      66
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     509    2046 
   Size:        509    1537
 
MslI CAynn'nnrTG

Cuts at:      0     838    1936    2046 
   Size:        838    1098     110

  Fragments arranged by size: 

                1098     838     110
 
MspA1I CmG'CkG

Cuts at:      0       6    1575    1708    1785    1838    1983    2046 
   Size:          6    1569     133      77      53     145      63

  Fragments arranged by size: 

                1569     145     133      77      63      53       6
 
NaeI GCC'GGC

Cuts at:      0     516    1284    1463    1728    1925    2046 
   Size:        516     768     179     265     197     121

  Fragments arranged by size: 

                 768     516     265     197     179     121
 
NarI GG'CG_CC

Cuts at:      0    1637    1730    1796    2046 
   Size:       1637      93      66     250

  Fragments arranged by size: 

                1637     250      93      66
 
NcoI C'CATG_G

Cuts at:      0    1277    2046 
   Size:       1277     769
 
NgoMIV G'CCGG_C

Cuts at:      0     514    1282    1461    1726    1923    2046 
   Size:        514     768     179     265     197     123

  Fragments arranged by size: 

                 768     514     265     197     179     123
 
NotI GC'GGCC_GC

Cuts at:      0    1709    2046 
   Size:       1709     337
 
NsiI A_TGCA'T

Cuts at:      0     866    2046 
   Size:        866    1180
 
NspI r_CATG'y

Cuts at:      0     699    1015    2046 
   Size:        699     316    1031

  Fragments arranged by size: 

                1031     699     316
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     788    2046 
   Size:        788    1258
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1078    1110    2046 
   Size:       1078      32     936

  Fragments arranged by size: 

                1078     936      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    1422    1821    2046 
   Size:       1422     399     225

  Fragments arranged by size: 

                1422     399     225
 
PspOMI G'GGCC_C

Cuts at:      0    1265    1353    2046 
   Size:       1265      88     693

  Fragments arranged by size: 

                1265     693      88
 
PstI C_TGCA'G

Cuts at:      0     306    1298    1580    1587    2046 
   Size:        306     992     282       7     459

  Fragments arranged by size: 

                 992     459     306     282       7
 
PvuII CAG'CTG

Cuts at:      0       6    1838    2046 
   Size:          6    1832     208

  Fragments arranged by size: 

                1832     208       6
 
RsrII CG'GwC_CG

Cuts at:      0     391    1371    2046 
   Size:        391     980     675

  Fragments arranged by size: 

                 980     675     391
 
SacI G_AGCT'C

Cuts at:      0     561    2046 
   Size:        561    1485
 
SacII CC_GC'GG

Cuts at:      0    1709    2046 
   Size:       1709     337
 
SbfI CC_TGCA'GG

Cuts at:      0    1298    2046 
   Size:       1298     748
 
ScaI AGT'ACT

Cuts at:      0    1657    2046 
   Size:       1657     389
 
SfcI C'TryA_G

Cuts at:      0     302    1294    1576    1583    2046 
   Size:        302     992     282       7     463

  Fragments arranged by size: 

                 992     463     302     282       7
 
SfoI GGC'GCC

Cuts at:      0    1638    1731    1797    2046 
   Size:       1638      93      66     249

  Fragments arranged by size: 

                1638     249      93      66
 
SmaI CCC'GGG

Cuts at:      0    1263    1340    1359    1427    2046 
   Size:       1263      77      19      68     619

  Fragments arranged by size: 

                1263     619      77      68      19
 
SmlI C'TyrA_G

Cuts at:      0    1960    2046 
   Size:       1960      86
 
SspI AAT'ATT

Cuts at:      0     753    2046 
   Size:        753    1293
 
StyI C'CwwG_G

Cuts at:      0     212     552    1277    1322    1817    2006    2046 
   Size:        212     340     725      45     495     189      40

  Fragments arranged by size: 

                 725     495     340     212     189      45      40
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     598    2046 
   Size:        598    1448
 
TatI w'GTAC_w

Cuts at:      0    1655    2046 
   Size:       1655     391
 
Tth111I GACn'n_nGTC

Cuts at:      0     265    2046 
   Size:        265    1781
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     132    1473    1740    2046 
   Size:        132    1341     267     306

  Fragments arranged by size: 

                1341     306     267     132
 
XmaI C'CCGG_G

Cuts at:      0    1261    1338    1357    1425    2046 
   Size:       1261      77      19      68     621

  Fragments arranged by size: 

                1261     621      77      68      19
 
ZraI GAC'GTC

Cuts at:      0     231    2046 
   Size:        231    1815
 

 Enzymes that do cut:

    AatII     AfeI     AleI     ApaI     ApoI     AscI     AvaI     BaeI
    BamHI     BanI    BanII     BbeI     BcgI    BciVI     BclI    BfrBI
     BglI Bme1580I     BmrI     BplI     BpmI    BpuEI     BsaI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
    BsmBI Bsp1286I    BspEI    BsrBI    BsrFI   BssHII   BstAPI    BstYI
     BtgI    BtgZI     BtsI     DrdI     EaeI     EagI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I     FalI    HaeII    Hin4I     KasI     MmeI
     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NotI     NsiI
     NspI    PflMI     PpiI    PpuMI   PspOMI     PstI    PvuII    RsrII
     SacI    SacII     SbfI     ScaI     SfcI     SfoI     SmaI     SmlI
     SspI     StyI    TaqII     TatI  Tth111I     XcmI     XmaI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AlfI     AloI    AlwNI    ApaLI     AseI    AsiSI    AvrII
     BbsI    BbvCI    BglII     BlpI    BmgBI     BmtI   Bpu10I    BsaAI
    BsiWI     BsmI    BspHI    BspMI    BsrDI    BsrGI    BssSI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I     ClaI    CspCI     DraI   DraIII
     EarI    EcoRI    EcoRV     FseI     FspI    FspAI   HincII  HindIII
     HpaI     KpnI     MfeI     MluI     MscI     NdeI     NheI     NruI
     PacI     PasI     PciI     PfoI     PmeI     PmlI    PshAI     PsiI
    PspXI     PsrI     PvuI     SalI    SanDI     SapI    SexAI     SfiI
     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     StuI     SwaI
     TstI     XbaI     XhoI     XmnI
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