Restriction map for pF1KB7338
 (Linear) (Six-base) MAPSORT of: KIBB7338.seq  Check: 4985  from: 1  to: 3189



KIBB7338.orf

 With 182 enzymes: SgfI * 

                        July 22, 2009 17:02  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     517    2346    3189 
   Size:        517    1829     843

  Fragments arranged by size: 

                1829     843     517
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     516    1095    3189 
   Size:        516     579    2094

  Fragments arranged by size: 

                2094     579     516
 
AflIII A'CryG_T

Cuts at:      0    2724    3189 
   Size:       2724     465
 
AgeI A'CCGG_T

Cuts at:      0    1452    3189 
   Size:       1452    1737
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    3056    3088    3189 
   Size:       3056      32     101

  Fragments arranged by size: 

                3056     101      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     814    1479    3189 
   Size:        814     665    1710

  Fragments arranged by size: 

                1710     814     665
 
ApaI G_GGCC'C

Cuts at:      0    2865    3189 
   Size:       2865     324
 
ApoI r'AATT_y

Cuts at:      0     860     879     930    1045    1774    2551    2734    3189 
   Size:        860      19      51     115     729     777     183     455

  Fragments arranged by size: 

                 860     777     729     455     183     115      51      19
            
 
AvaI C'yCGr_G

Cuts at:      0       7      26    2543    3189 
   Size:          7      19    2517     646

  Fragments arranged by size: 

                2517     646      19       7
 
BanI G'GyrC_C

Cuts at:      0      39     228     505    1023    1135    3189 
   Size:         39     189     277     518     112    2054

  Fragments arranged by size: 

                2054     518     277     189     112      39
 
BanII G_rGCy'C

Cuts at:      0    1102    2432    2669    2752    2865    3189 
   Size:       1102    1330     237      83     113     324

  Fragments arranged by size: 

                1330    1102     324     237     113      83
 
BbeI G_GCGC'C

Cuts at:      0      43     232    3189 
   Size:         43     189    2957

  Fragments arranged by size: 

                2957     189      43
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1692    1934    3189 
   Size:       1692     242    1255

  Fragments arranged by size: 

                1692    1255     242
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     150     184    2253    2287    3189 
   Size:        150      34    2069      34     902

  Fragments arranged by size: 

                2069     902     150      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     796    1192    3189 
   Size:        796     396    1997

  Fragments arranged by size: 

                1997     796     396
 
BclI T'GATC_A

Cuts at:      0     683    3189 
   Size:        683    2506
 
BglI GCCn_nnn'nGGC

Cuts at:      0     109     158    3189 
   Size:        109      49    3031

  Fragments arranged by size: 

                3031     109      49
 
BlpI GC'TnA_GC

Cuts at:      0    2668    3189 
   Size:       2668     521
 
Bme1580I G_kGCm'C

Cuts at:      0    1026    2865    3189 
   Size:       1026    1839     324

  Fragments arranged by size: 

                1839    1026     324
 
BmgBI CAC'GTC

Cuts at:      0     126    2242    3189 
   Size:        126    2116     947

  Fragments arranged by size: 

                2116     947     126
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     493     839    2421    3189 
   Size:        493     346    1582     768

  Fragments arranged by size: 

                1582     768     493     346
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     486     758    2539    2860    3189 
   Size:        486     272    1781     321     329

  Fragments arranged by size: 

                1781     486     329     321     272
 
Bpu10I CC'TnA_GC

Cuts at:      0    2663    3090    3189 
   Size:       2663     427      99

  Fragments arranged by size: 

                2663     427      99
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2266    3189 
   Size:       2266     923
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     629    1109    1175    3189 
   Size:        629     480      66    2014

  Fragments arranged by size: 

                2014     629     480      66
 
BsaBI GATnn'nnATC

Cuts at:      0    1682    1976    2000    3189 
   Size:       1682     294      24    1189

  Fragments arranged by size: 

                1682    1189     294      24
 
BsaHI Gr'CG_yC

Cuts at:      0      40     200     229    3189 
   Size:         40     160      29    2960

  Fragments arranged by size: 

                2960     160      40      29
 
BsaWI w'CCGG_w

Cuts at:      0     579    1342    1452    3189 
   Size:        579     763     110    1737

  Fragments arranged by size: 

                1737     763     579     110
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     401     431    1143    1173    3189 
   Size:        401      30     712      30    2016

  Fragments arranged by size: 

                2016     712     401      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     884    1096    1379    2384    2440    2455    2458    2837 
   Size:        884     212     283    1005      56      15       3     379

Cuts at:   2837    3122    3189 
   Size:        285      67

  Fragments arranged by size: 

                1005     884     379     285     283     212      67      56
                  15       3
 
BseYI C'CCAG_C

Cuts at:      0      78     817    1472    3189 
   Size:         78     739     655    1717

  Fragments arranged by size: 

                1717     739     655      78
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     336    1468    2464    2822    3189 
   Size:        336    1132     996     358     367

  Fragments arranged by size: 

                1132     996     367     358     336
 
BsiEI CG_ry'CG

Cuts at:      0      74    3189 
   Size:         74    3115
 
BsiHKAI G_wGCw'C

Cuts at:      0     453     876    1102    1259    2669    3189 
   Size:        453     423     226     157    1410     520

  Fragments arranged by size: 

                1410     520     453     423     226     157
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1135    3189 
   Size:       1135    2054
 
Bsp1286I G_dGCh'C

Cuts at:      0     453     876    1026    1102    1259    2432    2669    2752 
   Size:        453     423     150      76     157    1173     237      83

Cuts at:   2752    2865    3189 
   Size:        113     324

  Fragments arranged by size: 

                1173     453     423     324     237     157     150     113
                  83      76
 
BspEI T'CCGG_A

Cuts at:      0     579    1342    3189 
   Size:        579     763    1847

  Fragments arranged by size: 

                1847     763     579
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     517    2346    3189 
   Size:        517    1829     843

  Fragments arranged by size: 

                1829     843     517
 
BsrDI GCAATG_nn'

Cuts at:      0     576     707    1208    1592    3189 
   Size:        576     131     501     384    1597

  Fragments arranged by size: 

                1597     576     501     384     131
 
BsrFI r'CCGG_y

Cuts at:      0    1452    3189 
   Size:       1452    1737
 
BsrGI T'GTAC_A

Cuts at:      0     143    2721    2770    3189 
   Size:        143    2578      49     419

  Fragments arranged by size: 

                2578     419     143      49
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    2169    3189 
   Size:       2169    1020
 
BstYI r'GATC_y

Cuts at:      0    1124    3189 
   Size:       1124    2065
 
Bsu36I CC'TnA_GG

Cuts at:      0     736    3134    3182    3189 
   Size:        736    2398      48       7

  Fragments arranged by size: 

                2398     736      48       7
 
BtgI C'CryG_G

Cuts at:      0      18      68     179     296    3189 
   Size:         18      50     111     117    2893

  Fragments arranged by size: 

                2893     117     111      50      18
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1678    3189 
   Size:       1678    1511
 
BtsI GCAGTG_nn'

Cuts at:      0     319     686    2342    2602    2810    3189 
   Size:        319     367    1656     260     208     379

  Fragments arranged by size: 

                1656     379     367     319     260     208
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1300    1335    3189 
   Size:       1300      35    1854

  Fragments arranged by size: 

                1854    1300      35
 
DraI TTT'AAA

Cuts at:      0    1839    3189 
   Size:       1839    1350
 
EaeI y'GGCC_r

Cuts at:      0      71    3189 
   Size:         71    3118
 
EagI C'GGCC_G

Cuts at:      0      71    3189 
   Size:         71    3118
 
EarI CTCTTCn'nnn_

Cuts at:      0    1114    1675    2075    2417    2707    3189 
   Size:       1114     561     400     342     290     482

  Fragments arranged by size: 

                1114     561     482     400     342     290
 
EcoICRI GAG'CTC

Cuts at:      0    1100    2667    3189 
   Size:       1100    1567     522

  Fragments arranged by size: 

                1567    1100     522
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     486     516     758    1095    2539    2860    3189 
   Size:        486      30     242     337    1444     321     329

  Fragments arranged by size: 

                1444     486     337     329     321     242      30
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1229    3189 
   Size:       1229    1960
 
EcoO109I rG'GnC_Cy

Cuts at:      0     495     652    2587    3189 
   Size:        495     157    1935     602

  Fragments arranged by size: 

                1935     602     495     157
 
EcoRI G'AATT_C

Cuts at:      0     860    1774    2734    3189 
   Size:        860     914     960     455

  Fragments arranged by size: 

                 960     914     860     455
 
EcoRV GAT'ATC

Cuts at:      0     258     705     732    3189 
   Size:        258     447      27    2457

  Fragments arranged by size: 

                2457     447     258      27
 
FspI TGC'GCA

Cuts at:      0     311    3189 
   Size:        311    2878
 
HaeII r_GCGC'y

Cuts at:      0      43     232     539    2675    3189 
   Size:         43     189     307    2136     514

  Fragments arranged by size: 

                2136     514     307     189      43
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     376     408     668     700    1485    1517    1774    1806 
   Size:        376      32     260      32     785      32     257      32

Cuts at:   1806    3189 
   Size:       1383

  Fragments arranged by size: 

                1383     785     376     260     257      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0     990    3189 
   Size:        990    2199
 
HpaI GTT'AAC

Cuts at:      0     990    3189 
   Size:        990    2199
 
KasI G'GCGC_C

Cuts at:      0      39     228    3189 
   Size:         39     189    2961

  Fragments arranged by size: 

                2961     189      39
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    2076    2318    2430    3189 
   Size:       2076     242     112     759

  Fragments arranged by size: 

                2076     759     242     112
 
MslI CAynn'nnrTG

Cuts at:      0    1219    1912    2471    2856    3189 
   Size:       1219     693     559     385     333

  Fragments arranged by size: 

                1219     693     559     385     333
 
MspA1I CmG'CkG

Cuts at:      0      20      45      70     149     298    3162    3189 
   Size:         20      25      25      79     149    2864      27

  Fragments arranged by size: 

                2864     149      79      27      25      25      20
 
NarI GG'CG_CC

Cuts at:      0      40     229    3189 
   Size:         40     189    2960

  Fragments arranged by size: 

                2960     189      40
 
NdeI CA'TA_TG

Cuts at:      0    2049    3189 
   Size:       2049    1140
 
NotI GC'GGCC_GC

Cuts at:      0      71    3189 
   Size:         71    3118
 
NspI r_CATG'y

Cuts at:      0    1333    2728    3189 
   Size:       1333    1395     461

  Fragments arranged by size: 

                1395    1333     461
 
PasI CC'CwG_GG

Cuts at:      0    2217    3189 
   Size:       2217     972
 
PciI A'CATG_T

Cuts at:      0    2724    3189 
   Size:       2724     465
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     401    3189 
   Size:        401    2788
 
PfoI T'CCnGG_A

Cuts at:      0    2516    3189 
   Size:       2516     673
 
PpuMI rG'GwC_Cy

Cuts at:      0     495     652    2587    3189 
   Size:        495     157    1935     602

  Fragments arranged by size: 

                1935     602     495     157
 
PsiI TTA'TAA

Cuts at:      0     626    1265    3189 
   Size:        626     639    1924

  Fragments arranged by size: 

                1924     639     626
 
PspOMI G'GGCC_C

Cuts at:      0    2861    3189 
   Size:       2861     328
 
PstI C_TGCA'G

Cuts at:      0    2597    3189 
   Size:       2597     592
 
PvuII CAG'CTG

Cuts at:      0    3162    3189 
   Size:       3162      27
 
SacI G_AGCT'C

Cuts at:      0    1102    2669    3189 
   Size:       1102    1567     520

  Fragments arranged by size: 

                1567    1102     520
 
SacII CC_GC'GG

Cuts at:      0      21      71     299    3189 
   Size:         21      50     228    2890

  Fragments arranged by size: 

                2890     228      50      21
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1675    3189 
   Size:       1675    1514
 
SfcI C'TryA_G

Cuts at:      0    1463    1875    2348    2593    3189 
   Size:       1463     412     473     245     596

  Fragments arranged by size: 

                1463     596     473     412     245
 
SfoI GGC'GCC

Cuts at:      0      41     230    3189 
   Size:         41     189    2959

  Fragments arranged by size: 

                2959     189      41
 
SmaI CCC'GGG

Cuts at:      0       9      28    2545    3189 
   Size:          9      19    2517     644

  Fragments arranged by size: 

                2517     644      19       9
 
SmlI C'TyrA_G

Cuts at:      0    2245    3189 
   Size:       2245     944
 
SspI AAT'ATT

Cuts at:      0    1865    2487    3189 
   Size:       1865     622     702

  Fragments arranged by size: 

                1865     702     622
 
StuI AGG'CCT

Cuts at:      0    1847    3189 
   Size:       1847    1342
 
StyI C'CwwG_G

Cuts at:      0     155     289     798    2181    3189 
   Size:        155     134     509    1383    1008

  Fragments arranged by size: 

                1383    1008     509     155     134
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1399    1502    3163    3189 
   Size:       1399     103    1661      26

  Fragments arranged by size: 

                1661    1399     103      26
 
TatI w'GTAC_w

Cuts at:      0     143    2141    2721    2770    3189 
   Size:        143    1998     580      49     419

  Fragments arranged by size: 

                1998     580     419     143      49
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     401     425     433     457    1319    1351    2444    2476 
   Size:        401      24       8      24     862      32    1093      32

Cuts at:   2476    3189 
   Size:        713

  Fragments arranged by size: 

                1093     862     713     401      32      32      24      24
                   8
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1129    2520    3189 
   Size:       1129    1391     669

  Fragments arranged by size: 

                1391    1129     669
 
XmaI C'CCGG_G

Cuts at:      0       7      26    2543    3189 
   Size:          7      19    2517     646

  Fragments arranged by size: 

                2517     646      19       7
 
XmnI GAAnn'nnTTC

Cuts at:      0    2555    3189 
   Size:       2555     634
 

 Enzymes that do cut:

     AarI     AcuI   AflIII     AgeI     AloI    AlwNI     ApaI     ApoI
     AvaI     BanI    BanII     BbeI     BbsI     BcgI    BciVI     BclI
     BglI     BlpI Bme1580I    BmgBI     BmrI     BpmI   Bpu10I    BpuEI
     BsaI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspEI    BspMI    BsrDI    BsrFI
    BsrGI    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI    CspCI
     DraI     EaeI     EagI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI    EcoRV     FspI    HaeII    Hin4I   HincII     HpaI     KasI
     MmeI     MslI   MspA1I     NarI     NdeI     NotI     NspI     PasI
     PciI    PflMI     PfoI    PpuMI     PsiI   PspOMI     PstI    PvuII
     SacI    SacII     SapI     SfcI     SfoI     SmaI     SmlI     SspI
     StuI     StyI    TaqII     TatI     TstI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII     AhdI     AleI
     AlfI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
    BbvCI    BfrBI    BglII     BmtI     BplI    BsaAI    BsiWI     BsmI
    BspHI    BsrBI   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I
     ClaI   DraIII     DrdI     EciI     FalI     FseI    FspAI  HindIII
     KpnI     MfeI     MluI     MscI     NaeI     NcoI   NgoMIV     NheI
     NruI     NsiI     PacI     PmeI     PmlI     PpiI    PshAI    PspXI
     PsrI     PvuI    RsrII     SalI    SanDI     SbfI     ScaI    SexAI
     SfiI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     SwaI
  Tth111I     XbaI     XhoI     ZraI
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