Restriction map for pF1KE1086
##################################
# NO CUT ENZYMES (   59 enzymes) #
##################################

AatII       AhdI        Aor51HI     AscI        AspEI       
AviII       AvrII       BamHI       BbeI        BbrPI       
BglI        BlnI        BlpI        Bpu1102I    BsgI        
BsiWI       BspDI       BssHII      Bst1107I    BstZ17I     
CelII       ClaI        CpoI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      FseI        FspI        
KasI        KspI        MluI        NaeI        NarI        
NgoMIV      NotI        NruI        PacI        PaeR7I      
PmaCI       PmeI        PmlI        PshAI       RsrII       
SacII       SalI        SbfI        SexAI       SfiI        
SfoI        SgrAI       SmaI        SnaBI       Sse8387I    
StuI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   55 enzymes) #
###################################

AccII        6087  CGCG                 CG^CG
AccIII       5708  TCCGGA               T^CCGGA
AclI         2938  AACGTT               AA^CGTT
AcyI         1292  GGCGTC               G[GA]^CG[CT]C
AgeI         6684  ACCGGT               A^CCGGT
Alw44I       1391  GTGCAC               G^TGCAC
AocI         5971  CCTGAGG              CC^T[ACGT]AGG
ApaLI        1391  GTGCAC               G^TGCAC
AseI         7033  ATTAAT               AT^TAAT
AsnI         7033  ATTAAT               AT^TAAT
AspI         2101  GACATTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI         3327  CCCGAG               C^[CT]CG[GA]G
BalI          511  TGGCCA               TGG^CCA
BcgI          314  CGAGACTTCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI         2001  CCGGG                CC^[GC]GG
BglII        6220  AGATCT               A^GATCT
BsaHI        1292  GGCGTC               G[GA]^CG[CT]C
BseAI        5708  TCCGGA               T^CCGGA
BsiEI        6330  CGATCG               CG[GA][CT]^CG
BsoBI        3327  CCCGAG               C^[CT]CG[GA]G
BspEI        5708  TCCGGA               T^CCGGA
BsrBI        6324  CCGCTC               CCGCTC(-3/-3)
BsrFI        6684  ACCGGT               [GA]^CCGG[CT]
BssSI        4955  CACGAG               CACGAG(-5/-1)
BstUI        6087  CGCG                 CG^CG
BstXI        3374  CCAAATTGTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I       5971  CCTGAGG              CC^T[ACGT]AGG
CfoI         7723  GCGC                 GCG^C
Cfr10I       6684  ACCGGT               [GA]^CCGG[CT]
DrdI         6537  GACAGCTGTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       5971  CCTGAGG              CC^T[ACGT]AGG
EcoNI        6993  CCTGAGAAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I      5047  ATGCAT               ATGCA^T
HaeII        7722  AGCGCC               [GA]GCGC^[CT]
HgaI         1293  GCGTC                GACGC(5/10)
HhaI         7723  GCGC                 GCG^C
Hin1I        1292  GGCGTC               G[GA]^CG[CT]C
HinP1I       7723  GCGC                 G^CGC
HpaI         6270  GTTAAC               GTT^AAC
MluNI         511  TGGCCA               TGG^CCA
MroI         5708  TCCGGA               T^CCGGA
MscI          511  TGGCCA               TGG^CCA
MvnI         6087  CGCG                 CG^CG
NciI         2001  CCGGG                CC^[GC]GG
NsiI         5047  ATGCAT               ATGCA^T
PflFI        2101  GACATTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        6853  CCATTTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI        6684  ACCGGT               A^CCGGT
PshBI        7033  ATTAAT               AT^TAAT
Psp1406I     2938  AACGTT               AA^CGTT
PvuI         6330  CGATCG               CGAT^CG
SphI         5277  GCATGC               GCATG^C
SwaI          950  ATTTAAAT             ATTT^AAAT
Tth111I      2101  GACATTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       6853  CCATTTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

Acc65I       2652  GGTACC               G^GTACC
             6823  GGTACC              
Asp718I      2652  GGTACC               G^GTACC
             6823  GGTACC              
BanI         2652  GGTACC               G^G[CT][GA]CC
             6823  GGTACC              
BbvCI        1257  CCTCAGC              CCTCAGC(-5/-2)
             5142  GCTGAGG             
BclI         3768  TGATCA               T^GATCA
             7632  TGATCA              
BsaAI        5313  CACGTA               [CT]AC^GT[GA]
             5866  TACGTG              
BsaBI        2956  GATCTTAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             3313  GATCCATATC          
BseRI        1225  GAGGAG               GAGGAG(10/8)
             4329  GAGGAG              
BsmBI        1297  CGTCTC               CGTCTC(1/5)
             1307  CGTCTC              
Bsp1407I       87  TGTACA               T^GTACA
             6336  TGTACA              
BspMI        4534  ACCTGC               ACCTGC(4/8)
             4483  GCAGGT              
BsrGI          87  TGTACA               T^GTACA
             6336  TGTACA              
BstAPI       3016  GCAAGCCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             3025  GCAGCTTCTGC         
BstEII       4315  GGTCACC              G^GT[ACGT]ACC
             5803  GGTCACC             
BstPI        4315  GGTCACC              G^GT[ACGT]ACC
             5803  GGTCACC             
DraIII       1901  CACGATGTG            CAC[ACGT][ACGT][ACGT]^GTG
             4764  CACACAGTG           
EcoO65I      4315  GGTCACC              G^GT[ACGT]ACC
             5803  GGTCACC             
EcoRV        3334  GATATC               GAT^ATC
             5443  GATATC              
FauI           37  CCCGC                CCCGC(4/6)
               45  GCGGG               
FbaI         3768  TGATCA               T^GATCA
             7632  TGATCA              
KpnI         2652  GGTACC               GGTAC^C
             6823  GGTACC              
MamI         2956  GATCTTAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             3313  GATCCATATC          
NheI          796  GCTAGC               G^CTAGC
             6841  GCTAGC              
PpuMI        3486  GGGACCC              [GA]G^G[AT]CC[CT]
             7569  GGGTCCT             
SapI          874  GAAGAGC              GCTCTTC(1/4)
             7598  GAAGAGC             
ScaI         1169  AGTACT               AGT^ACT
             6131  AGTACT              
SpeI         5472  ACTAGT               A^CTAGT
             6012  ACTAGT              
SspBI          87  TGTACA               T^GTACA
             6336  TGTACA              
XbaI         4896  TCTAGA               T^CTAGA
             6666  TCTAGA              
XcmI         1685  CCATTGATAAGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             7902  CCAGATGTATCTTGG     


#########################################
# 3 OR MORE CUT ENZYMES (  114 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AflII       
AflIII      AluI        AlwI        AlwNI       ApaI        
ApoI        Asp700I     AspHI       AvaII       BanII       
BbsI        BbvI        BciVI       BfaI        BfrI        
BmyI        BpmI        BpuAI       BsaI        BsaJI       
BsaWI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsmI        Bsp1286I    BspHI       BspLU11I    
BsrDI       BsrI        BssKI       BstBI       BstF5I      
BstNI       BstYI       Cac8I       Cfr13I      DdeI        
DpnII       DraI        DraII       DsaI        EaeI        
EarI        EcoO109I    EcoRI       EcoRII      EcoT14I     
Fnu4HI      FokI        HaeIII      HapII       HincII      
HindII      HindIII     HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MfeI        MflI        MnlI        
MseI        MslI        MspA1I      MspI        MunI        
MvaI        MwoI        NcoI        NdeI        NdeII       
NlaIII      NlaIV       NspI        NspV        PleI        
PstI        PvuII       RcaI        RsaI        SacI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SfuI        SmlI        SspI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XhoII       XmnI        
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