Restriction map for pF1KE1086
 (Linear) (Six-base) MAPSORT of: KIEE1086.seq  Check: 5158  from: 1  to: 7932



KIEE1086.orf

 With 182 enzymes: SgfI * 

                        July 16, 2019 14:21  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    4543    7932 
   Size:       4543    3389
 
AccI GT'mk_AC

Cuts at:      0    4841    5248    7008    7932 
   Size:       4841     407    1760     924

  Fragments arranged by size: 

                4841    1760     924     407
 
Acc65I G'GTAC_C

Cuts at:      0    2652    6823    7932 
   Size:       2652    4171    1109

  Fragments arranged by size: 

                4171    2652    1109
 
AclI AA'CG_TT

Cuts at:      0    2939    7932 
   Size:       2939    4993
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1402    2531    2614    3187    3826    3838    6901    7932 
   Size:       1402    1129      83     573     639      12    3063    1031

  Fragments arranged by size: 

                3063    1402    1129    1031     639     573      83      12
            
 
AflII C'TTAA_G

Cuts at:      0    1009    1630    2541    4437    7044    7932 
   Size:       1009     621     911    1896    2607     888

  Fragments arranged by size: 

                2607    1896    1009     911     888     621
 
AflIII A'CryG_T

Cuts at:      0    1356    2208    3495    5312    5564    5984    7473    7854 
   Size:       1356     852    1287    1817     252     420    1489     381

Cuts at:   7854    7932 
   Size:         78

  Fragments arranged by size: 

                1817    1489    1356    1287     852     420     381     252
                  78
 
AgeI A'CCGG_T

Cuts at:      0    6684    7932 
   Size:       6684    1248
 
AleI CACnn'nnGTG

Cuts at:      0    1624    5263    7932 
   Size:       1624    3639    2669

  Fragments arranged by size: 

                3639    2669    1624
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1053    7932 
   Size:       1053    6879
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1668    2704    3031    3414    4794    7932 
   Size:       1668    1036     327     383    1380    3138

  Fragments arranged by size: 

                3138    1668    1380    1036     383     327
 
ApaI G_GGCC'C

Cuts at:      0    1183    1977    3328    7932 
   Size:       1183     794    1351    4604

  Fragments arranged by size: 

                4604    1351    1183     794
 
ApaLI G'TGCA_C

Cuts at:      0    1391    7932 
   Size:       1391    6541
 
ApoI r'AATT_y

Cuts at:      0     104     292     600     607    1063    1703    2046    2116 
   Size:        104     188     308       7     456     640     343      70

Cuts at:   2116    2430    4526    4783    5673    6443    6902    7018    7276 
   Size:        314    2096     257     890     770     459     116     258

Cuts at:   7276    7932 
   Size:        656

  Fragments arranged by size: 

                2096     890     770     656     640     459     456     343
                 314     308     258     257     188     116     104      70
                   7
 
AseI AT'TA_AT

Cuts at:      0    7034    7932 
   Size:       7034     898
 
AvaI C'yCGr_G

Cuts at:      0    3327    7932 
   Size:       3327    4605
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    4374    4407    7341    7374    7932 
   Size:       4374      33    2934      33     558

  Fragments arranged by size: 

                4374    2934     558      33      33
 
BanI G'GyrC_C

Cuts at:      0    2652    6823    7932 
   Size:       2652    4171    1109

  Fragments arranged by size: 

                4171    2652    1109
 
BanII G_rGCy'C

Cuts at:      0    1183    1977    1987    2739    3328    3421    3628    4239 
   Size:       1183     794      10     752     589      93     207     611

Cuts at:   4239    7932 
   Size:       3693

  Fragments arranged by size: 

                3693    1183     794     752     611     589     207      93
                  10
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     467     995    4877    7932 
   Size:        467     528    3882    3055

  Fragments arranged by size: 

                3882    3055     528     467
 
BbvCI CC'TCA_GC

Cuts at:      0    1258    5143    7932 
   Size:       1258    3885    2789

  Fragments arranged by size: 

                3885    2789    1258
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     303     337    7932 
   Size:        303      34    7595

  Fragments arranged by size: 

                7595     303      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     122     843    2592    4908    7932 
   Size:        122     721    1749    2316    3024

  Fragments arranged by size: 

                3024    2316    1749     721     122
 
BclI T'GATC_A

Cuts at:      0    3768    7632    7932 
   Size:       3768    3864     300

  Fragments arranged by size: 

                3864    3768     300
 
BfrBI ATG'CAT

Cuts at:      0    5049    7932 
   Size:       5049    2883
 
BglII A'GATC_T

Cuts at:      0    6220    7932 
   Size:       6220    1712
 
Bme1580I G_kGCm'C

Cuts at:      0    1183    1395    1977    3156    3328    7932 
   Size:       1183     212     582    1179     172    4604

  Fragments arranged by size: 

                4604    1183    1179     582     212     172
 
BmgBI CAC'GTC

Cuts at:      0    2874    7932 
   Size:       2874    5058
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1980    3199    4621    5419    5655    7932 
   Size:       1980    1219    1422     798     236    2277

  Fragments arranged by size: 

                2277    1980    1422    1219     798     236
 
BmtI G_CTAG'C

Cuts at:      0     800    6845    7932 
   Size:        800    6045    1087

  Fragments arranged by size: 

                6045    1087     800
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    7297    7932 
   Size:       7297     635
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      37    1696    2732    3787    4175    4436    5597    7760 
   Size:         37    1659    1036    1055     388     261    1161    2163

Cuts at:   7760    7932 
   Size:        172

  Fragments arranged by size: 

                2163    1659    1161    1055    1036     388     261     172
                  37
 
Bpu10I CC'TnA_GC

Cuts at:      0    1258    4340    5143    7932 
   Size:       1258    3082     803    2789

  Fragments arranged by size: 

                3082    2789    1258     803
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1966    4028    4112    4223    5612    7932 
   Size:       1966    2062      84     111    1389    2320

  Fragments arranged by size: 

                2320    2062    1966    1389     111      84
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    3814    4294    7152    7415    7932 
   Size:       3814     480    2858     263     517

  Fragments arranged by size: 

                3814    2858     517     480     263
 
BsaAI yAC'GTr

Cuts at:      0    5315    5868    7932 
   Size:       5315     553    2064

  Fragments arranged by size: 

                5315    2064     553
 
BsaBI GATnn'nnATC

Cuts at:      0    2960    3317    7932 
   Size:       2960     357    4615

  Fragments arranged by size: 

                4615    2960     357
 
BsaHI Gr'CG_yC

Cuts at:      0    1293    7932 
   Size:       1293    6639
 
BsaWI w'CCGG_w

Cuts at:      0     125    5708    6194    6684    7932 
   Size:        125    5583     486     490    1248

  Fragments arranged by size: 

                5583    1248     490     486     125
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    2636    2666    7733    7763    7932 
   Size:       2636      30    5067      30     169

  Fragments arranged by size: 

                5067    2636     169      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1240    4344    7932 
   Size:       1240    3104    3588

  Fragments arranged by size: 

                3588    3104    1240
 
BseYI C'CCAG_C

Cuts at:      0      51    1075    7932 
   Size:         51    1024    6857

  Fragments arranged by size: 

                6857    1024      51
 
BsiEI CG_ry'CG

Cuts at:      0    6333    7932 
   Size:       6333    1599
 
BsiHKAI G_wGCw'C

Cuts at:      0    1001    1332    1395    1987    2739    3421    3835    4239 
   Size:       1001     331      63     592     752     682     414     404

Cuts at:   4239    6721    7932 
   Size:       2482    1211

  Fragments arranged by size: 

                2482    1211    1001     752     682     592     414     404
                 331      63
 
BsmI GAATG_Cn'

Cuts at:      0    1781    2871    5607    5770    7471    7713    7932 
   Size:       1781    1090    2736     163    1701     242     219

  Fragments arranged by size: 

                2736    1781    1701    1090     242     219     163
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1303    1313    7932 
   Size:       1303      10    6619

  Fragments arranged by size: 

                6619    1303      10
 
Bsp1286I G_dGCh'C

Cuts at:      0    1001    1183    1332    1395    1977    1987    2739    3156 
   Size:       1001     182     149      63     582      10     752     417

Cuts at:   3156    3328    3421    3628    3835    4239    6721    7932 
   Size:        172      93     207     207     404    2482    1211

  Fragments arranged by size: 

                2482    1211    1001     752     582     417     404     207
                 207     182     172     149      93      63      10
 
BspEI T'CCGG_A

Cuts at:      0    5708    7932 
   Size:       5708    2224
 
BspHI T'CATG_A

Cuts at:      0     224     521    3747    6794    6968    7308    7932 
   Size:        224     297    3226    3047     174     340     624

  Fragments arranged by size: 

                3226    3047     624     340     297     224     174
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    4474    4543    7932 
   Size:       4474      69    3389

  Fragments arranged by size: 

                4474    3389      69
 
BsrBI CCG'CTC

Cuts at:      0    6326    7932 
   Size:       6326    1606
 
BsrDI GCAATG_nn'

Cuts at:      0    4703    6060    6530    7932 
   Size:       4703    1357     470    1402

  Fragments arranged by size: 

                4703    1402    1357     470
 
BsrFI r'CCGG_y

Cuts at:      0    6684    7932 
   Size:       6684    1248
 
BsrGI T'GTAC_A

Cuts at:      0      87    6336    7932 
   Size:         87    6249    1596

  Fragments arranged by size: 

                6249    1596      87
 
BssSI C'ACGA_G

Cuts at:      0    4955    7932 
   Size:       4955    2977
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    3022    3031    7932 
   Size:       3022       9    4901

  Fragments arranged by size: 

                4901    3022       9
 
BstBI TT'CG_AA

Cuts at:      0    3042    7086    7596    7932 
   Size:       3042    4044     510     336

  Fragments arranged by size: 

                4044    3042     510     336
 
BstEII G'GTnAC_C

Cuts at:      0    4315    5803    7932 
   Size:       4315    1488    2129

  Fragments arranged by size: 

                4315    2129    1488
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    3381    7932 
   Size:       3381    4551
 
BstYI r'GATC_y

Cuts at:      0    2442    4092    5114    6220    7932 
   Size:       2442    1650    1022    1106    1712

  Fragments arranged by size: 

                2442    1712    1650    1106    1022
 
Bsu36I CC'TnA_GG

Cuts at:      0    5972    7932 
   Size:       5972    1960
 
BtgI C'CryG_G

Cuts at:      0    1789    5208    5994    6152    7932 
   Size:       1789    3419     786     158    1780

  Fragments arranged by size: 

                3419    1789    1780     786     158
 
BtsI GCAGTG_nn'

Cuts at:      0      63    5153    7145    7379    7932 
   Size:         63    5090    1992     234     553

  Fragments arranged by size: 

                5090    1992     553     234      63
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    6508    6543    7932 
   Size:       6508      35    1389

  Fragments arranged by size: 

                6508    1389      35
 
DraI TTT'AAA

Cuts at:      0     402     953    1823    2915    6729    6941    7932 
   Size:        402     551     870    1092    3814     212     991

  Fragments arranged by size: 

                3814    1092     991     870     551     402     212
 
DraIII CAC_nnn'GTG

Cuts at:      0    1906    4769    7932 
   Size:       1906    2863    3163

  Fragments arranged by size: 

                3163    2863    1906
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    6543    7932 
   Size:       6543    1389
 
EaeI y'GGCC_r

Cuts at:      0     511    5066    6321    7932 
   Size:        511    4555    1255    1611

  Fragments arranged by size: 

                4555    1611    1255     511
 
EarI CTCTTCn'nnn_

Cuts at:      0     869    2156    2224    3857    6920    7065    7593    7932 
   Size:        869    1287      68    1633    3063     145     528     339

  Fragments arranged by size: 

                3063    1633    1287     869     528     339     145      68
            
 
EcoICRI GAG'CTC

Cuts at:      0    1985    2737    3419    4237    7932 
   Size:       1985     752     682     818    3695

  Fragments arranged by size: 

                3695    1985     818     752     682
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      37    1402    1696    2531    2614    2732    3187    3787 
   Size:         37    1365     294     835      83     118     455     600

Cuts at:   3787    3826    3838    4175    4436    5597    6901    7760    7932 
   Size:         39      12     337     261    1161    1304     859     172

  Fragments arranged by size: 

                1365    1304    1161     859     835     600     455     337
                 294     261     172     118      83      39      37      12
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    6997    7932 
   Size:       6997     935
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1974    3324    3487    7570    7932 
   Size:       1974    1350     163    4083     362

  Fragments arranged by size: 

                4083    1974    1350     362     163
 
EcoRI G'AATT_C

Cuts at:      0     292    2046    5673    7018    7932 
   Size:        292    1754    3627    1345     914

  Fragments arranged by size: 

                3627    1754    1345     914     292
 
EcoRV GAT'ATC

Cuts at:      0    3336    5445    7932 
   Size:       3336    2109    2487

  Fragments arranged by size: 

                3336    2487    2109
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    2431    2619    3760    3945    7932 
   Size:       2431     188    1141     185    3987

  Fragments arranged by size: 

                3987    2431    1141     188     185
 
HaeII r_GCGC'y

Cuts at:      0    7726    7932 
   Size:       7726     206
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     868     900    1264    1296    2012    2044    3325    3357 
   Size:        868      32     364      32     716      32    1281      32

Cuts at:   3357    3822    3854    4024    4056    4563    4595    4693    4725 
   Size:        465      32     170      32     507      32      98      32

Cuts at:   4725    5371    5403    7045    7077    7327    7359    7617    7649 
   Size:        646      32    1642      32     250      32     258      32

Cuts at:   7649    7733    7765    7932 
   Size:         84      32     167

  Fragments arranged by size: 

                1642    1281     868     716     646     507     465     364
                 258     250     170     167      98      84      32      32
                  32      32      32      32      32      32      32      32
                  32      32      32
 
HincII GTy'rAC

Cuts at:      0    1561    1829    2137    4403    4723    6272    7932 
   Size:       1561     268     308    2266     320    1549    1660

  Fragments arranged by size: 

                2266    1660    1561    1549     320     308     268
 
HindIII A'AGCT_T

Cuts at:      0     923    2276    3915    4170    5511    6661    7932 
   Size:        923    1353    1639     255    1341    1150    1271

  Fragments arranged by size: 

                1639    1353    1341    1271    1150     923     255
 
HpaI GTT'AAC

Cuts at:      0    6272    7932 
   Size:       6272    1660
 
KpnI G_GTAC'C

Cuts at:      0    2656    6827    7932 
   Size:       2656    4171    1105

  Fragments arranged by size: 

                4171    2656    1105
 
MfeI C'AATT_G

Cuts at:      0     653     859    4321    6430    7485    7932 
   Size:        653     206    3462    2109    1055     447

  Fragments arranged by size: 

                3462    2109    1055     653     447     206
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    5558    5739    6615    7897    7932 
   Size:       5558     181     876    1282      35

  Fragments arranged by size: 

                5558    1282     876     181      35
 
MscI TGG'CCA

Cuts at:      0     513    7932 
   Size:        513    7419
 
MslI CAynn'nnrTG

Cuts at:      0    1624    1788    1808    2207    3884    4549    5207    5263 
   Size:       1624     164      20     399    1677     665     658      56

Cuts at:   5263    6306    6568    6970    7932 
   Size:       1043     262     402     962

  Fragments arranged by size: 

                1677    1624    1043     962     665     658     402     399
                 262     164      56      20
 
MspA1I CmG'CkG

Cuts at:      0    1075    4249    5244    5506    6541    7932 
   Size:       1075    3174     995     262    1035    1391

  Fragments arranged by size: 

                3174    1391    1075    1035     995     262
 
NcoI C'CATG_G

Cuts at:      0    1789    5208    5994    6152    7932 
   Size:       1789    3419     786     158    1780

  Fragments arranged by size: 

                3419    1789    1780     786     158
 
NdeI CA'TA_TG

Cuts at:      0    6303    6608    7092    7932 
   Size:       6303     305     484     840

  Fragments arranged by size: 

                6303     840     484     305
 
NheI G'CTAG_C

Cuts at:      0     796    6841    7932 
   Size:        796    6045    1091

  Fragments arranged by size: 

                6045    1091     796
 
NsiI A_TGCA'T

Cuts at:      0    5051    7932 
   Size:       5051    2881
 
NspI r_CATG'y

Cuts at:      0    1360    2163    2212    2569    3408    3499    4348    5281 
   Size:       1360     803      49     357     839      91     849     933

Cuts at:   5281    5568    5988    7477    7607    7858    7932 
   Size:        287     420    1489     130     251      74

  Fragments arranged by size: 

                1489    1360     933     849     839     803     420     357
                 287     251     130      91      74      49
 
PciI A'CATG_T

Cuts at:      0    1356    2208    3495    5564    5984    7473    7854    7932 
   Size:       1356     852    1287    2069     420    1489     381      78

  Fragments arranged by size: 

                2069    1489    1356    1287     852     420     381      78
            
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    6859    7932 
   Size:       6859    1073
 
PfoI T'CCnGG_A

Cuts at:      0    1757    3803    6858    7932 
   Size:       1757    2046    3055    1074

  Fragments arranged by size: 

                3055    2046    1757    1074
 
PpuMI rG'GwC_Cy

Cuts at:      0    3487    7570    7932 
   Size:       3487    4083     362

  Fragments arranged by size: 

                4083    3487     362
 
PsiI TTA'TAA

Cuts at:      0    2524    7932 
   Size:       2524    5408
 
PspOMI G'GGCC_C

Cuts at:      0    1179    1973    3324    7932 
   Size:       1179     794    1351    4608

  Fragments arranged by size: 

                4608    1351    1179     794
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    2396    2428    5477    5509    7932 
   Size:       2396      32    3049      32    2423

  Fragments arranged by size: 

                3049    2423    2396      32      32
 
PstI C_TGCA'G

Cuts at:      0    1052    1982    3027    3693    5777    7386    7932 
   Size:       1052     930    1045     666    2084    1609     546

  Fragments arranged by size: 

                2084    1609    1052    1045     930     666     546
 
PvuI CG_AT'CG

Cuts at:      0    6333    7932 
   Size:       6333    1599
 
PvuII CAG'CTG

Cuts at:      0    1075    4249    5244    5506    6541    7932 
   Size:       1075    3174     995     262    1035    1391

  Fragments arranged by size: 

                3174    1391    1075    1035     995     262
 
SacI G_AGCT'C

Cuts at:      0    1987    2739    3421    4239    7932 
   Size:       1987     752     682     818    3693

  Fragments arranged by size: 

                3693    1987     818     752     682
 
SanDI GG'GwC_CC

Cuts at:      0    3487    7932 
   Size:       3487    4445
 
SapI GCTCTTCn'nnn_

Cuts at:      0     869    7593    7932 
   Size:        869    6724     339

  Fragments arranged by size: 

                6724     869     339
 
ScaI AGT'ACT

Cuts at:      0    1171    6133    7932 
   Size:       1171    4962    1799

  Fragments arranged by size: 

                4962    1799    1171
 
SfcI C'TryA_G

Cuts at:      0     155    1048    1586    1638    1863    1978    2223    3023 
   Size:        155     893     538      52     225     115     245     800

Cuts at:   3023    3689    4287    4539    5679    5773    7382    7932 
   Size:        666     598     252    1140      94    1609     550

  Fragments arranged by size: 

                1609    1140     893     800     666     598     550     538
                 252     245     225     155     115      94      52
 
SmlI C'TyrA_G

Cuts at:      0    1009    1630    1945    2541    4043    4127    4238    4437 
   Size:       1009     621     315     596    1502      84     111     199

Cuts at:   4437    5627    7044    7932 
   Size:       1190    1417     888

  Fragments arranged by size: 

                1502    1417    1190    1009     888     621     596     315
                 199     111      84
 
SpeI A'CTAG_T

Cuts at:      0    5472    6012    7932 
   Size:       5472     540    1920

  Fragments arranged by size: 

                5472    1920     540
 
SphI G_CATG'C

Cuts at:      0    5281    7932 
   Size:       5281    2651
 
SspI AAT'ATT

Cuts at:      0     936    1122    1940    5004    7440    7932 
   Size:        936     186     818    3064    2436     492

  Fragments arranged by size: 

                3064    2436     936     818     492     186
 
StyI C'CwwG_G

Cuts at:      0    1789    2501    3216    5133    5208    5687    5894    5994 
   Size:       1789     712     715    1917      75     479     207     100

Cuts at:   5994    6152    7932 
   Size:        158    1780

  Fragments arranged by size: 

                1917    1789    1780     715     712     479     207     158
                 100      75
 
SwaI ATTT'AAAT

Cuts at:      0     953    7932 
   Size:        953    6979
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1299    7125    7932 
   Size:       1299    5826     807

  Fragments arranged by size: 

                5826    1299     807
 
TatI w'GTAC_w

Cuts at:      0      87    1169    1507    2146    4729    6131    6336    7932 
   Size:         87    1082     338     639    2583    1402     205    1596

  Fragments arranged by size: 

                2583    1596    1402    1082     639     338     205      87
            
 
Tth111I GACn'n_nGTC

Cuts at:      0    2104    7932 
   Size:       2104    5828
 
XbaI T'CTAG_A

Cuts at:      0    4896    6666    7932 
   Size:       4896    1770    1266

  Fragments arranged by size: 

                4896    1770    1266
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1692    7909    7932 
   Size:       1692    6217      23

  Fragments arranged by size: 

                6217    1692      23
 
XmnI GAAnn'nnTTC

Cuts at:      0     764    1415    1658    2819    6699    6854    7439    7932 
   Size:        764     651     243    1161    3880     155     585     493

  Fragments arranged by size: 

                3880    1161     764     651     585     493     243     155
            
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AleI     AlfI    AlwNI     ApaI    ApaLI     ApoI     AseI     AvaI
     BaeI     BanI    BanII     BbsI    BbvCI     BcgI    BciVI     BclI
    BfrBI    BglII Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI   Bsu36I     BtgI     BtsI    CspCI
     DraI   DraIII     DrdI     EaeI     EarI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI    HaeII    Hin4I   HincII  HindIII
     HpaI     KpnI     MfeI     MmeI     MscI     MslI   MspA1I     NcoI
     NdeI     NheI     NsiI     NspI     PciI    PflMI     PfoI    PpuMI
     PsiI   PspOMI     PsrI     PstI     PvuI    PvuII     SacI    SanDI
     SapI     ScaI     SfcI     SmlI     SpeI     SphI     SspI     StyI
     SwaI    TaqII     TatI  Tth111I     XbaI     XcmI     XmnI

 Enzymes that do not cut: 

    AatII     AfeI     AhdI     AloI     AscI    AsiSI    AvrII    BamHI
     BbeI     BglI     BlpI     BsgI    BsiWI   BssHII  BstZ17I    BtgZI
     ClaI     EagI     EciI     FseI     FspI    FspAI     KasI     MluI
     NaeI     NarI   NgoMIV     NotI     NruI     PacI     PasI     PmeI
     PmlI     PpiI    PshAI    PspXI    RsrII    SacII     SalI     SbfI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SrfI
     StuI     TstI     XhoI     XmaI     ZraI
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