Restriction map for pF1KB9961
##################################
# NO CUT ENZYMES (   94 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AflII       
AgeI        Aor51HI     AscI        AseI        AsnI        
Asp700I     AviII       AvrII       BbrPI       BcgI        
BciVI       BclI        BfrI        BglII       BlnI        
BsaBI       BseAI       BsmBI       BsmI        Bsp1407I    
BspDI       BspEI       BspLU11I    BsrDI       BsrGI       
BssHII      Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstZ17I     ClaI        CpoI        DraI        
EarI        Eco47III    EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FseI        FspI        HgaI        
HindIII     HpaI        Ksp632I     MamI        MfeI        
MluI        MroI        MseI        MunI        NaeI        
NcoI        NdeI        NgoMIV      NheI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PinAI       PmaCI       PmeI        PmlI        PshBI       
Psp1406I    PvuI        RsrII       SalI        SapI        
ScaI        SfuI        SgrAI       SnaBI       SpeI        
SphI        SspBI       SspI        SwaI        TfiI        
Tru9I       XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

Acc65I        871  GGTACC               G^GTACC
AcsI         1276  AAATTC               [GA]^AATT[CT]
AflIII       1340  ACGTGT               A^C[GA][CT]GT
Alw44I         70  GTGCAC               G^TGCAC
ApaI          237  GGGCCC               GGGCC^C
ApaLI          70  GTGCAC               G^TGCAC
ApoI         1276  AAATTC               [GA]^AATT[CT]
Asp718I       871  GGTACC               G^GTACC
AspI          211  GACACCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI        1355  GGATCC               G^GATCC
BbsI         1243  GAAGAC               GAAGAC(2/6)
BfaI          290  CTAG                 C^TAG
BlpI          378  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      378  GCTCAGC              GC^T[ACGT]AGC
BpuAI        1243  GAAGAC               GAAGAC(2/6)
BsaI          541  GAGACC               GGTCTC(1/5)
BsaWI        1445  ACCGGA               [AT]^CCGG[AT]
BsiWI        1133  CGTACG               C^GTACG
BspHI         947  TCATGA               T^CATGA
BsrFI         247  GCCGGT               [GA]^CCGG[CT]
BssSI         526  CTCGTG               CACGAG(-5/-1)
CelII         378  GCTCAGC              GC^T[ACGT]AGC
Cfr10I        247  GCCGGT               [GA]^CCGG[CT]
DraIII        735  CACCGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          300  GACAGACCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HincII        340  GTCAAC               GT[CT]^[GA]AC
HindII        340  GTCAAC               GT[CT]^[GA]AC
KpnI          871  GGTACC               GGTAC^C
MaeI          290  CTAG                 C^TAG
NotI          169  GCGGCCGC             GC^GGCCGC
PflFI         211  GACACCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        1283  CCATCAAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI         473  GACGGGAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI          947  TCATGA               T^CATGA
SacI         1369  GAGCTC               GAGCT^C
SfiI          136  GGCCACCGCGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI         1594  CCCGGG               CCC^GGG
SmlI         1057  CTCAAG               C^T[CT][GA]AG
Tsp509I      1277  AATT                 ^AATT
Tth111I       211  GACACCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       1283  CCATCAAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI        1594  CCCGGG               C^CCGGG
XmaI         1594  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   38 enzymes) #
###################################

AcyI           41  GGCGCC               G[GA]^CG[CT]C
              420  GGCGCC              
AhdI          280  GACTATGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1339  GACGTGTGGTC         
AocI         1121  CCTCAGG              CC^T[ACGT]AGG
             1516  CCTGAGG             
AspEI         280  GACTATGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1339  GACGTGTGGTC         
AspHI          70  GTGCAC               G[AT]GC[AT]^C
             1369  GAGCTC              
BalI          773  TGGCCA               TGG^CCA
              884  TGGCCA              
BbeI           41  GGCGCC               GGCGC^C
              420  GGCGCC              
BglI          137  GCCACCGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              163  GCCCCCGCGGC         
BsaAI         415  TACGTG               [CT]AC^GT[GA]
             1135  TACGTG              
BsaHI          41  GGCGCC               G[GA]^CG[CT]C
              420  GGCGCC              
BsiEI         128  CGGCCG               CG[GA][CT]^CG
              170  CGGCCG              
BsiHKAI        70  GTGCAC               G[AT]GC[AT]^C
             1369  GAGCTC              
BspMI        1079  ACCTGC               ACCTGC(4/8)
             1541  ACCTGC              
Bsu36I       1121  CCTCAGG              CC^T[ACGT]AGG
             1516  CCTGAGG             
DsaI          141  CCGCGG               C^C[GA][CT]GG
              167  CCGCGG              
EagI          128  CGGCCG               C^GGCCG
              170  CGGCCG              
Eam1105I      280  GACTATGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1339  GACGTGTGGTC         
EclXI         128  CGGCCG               C^GGCCG
              170  CGGCCG              
Eco52I        128  CGGCCG               C^GGCCG
              170  CGGCCG              
Eco81I       1121  CCTCAGG              CC^T[ACGT]AGG
             1516  CCTGAGG             
EcoNI         771  CCTGGCCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              930  CCTGCAGGAGG         
Hin1I          41  GGCGCC               G[GA]^CG[CT]C
              420  GGCGCC              
KasI           41  GGCGCC               G^GCGCC
              420  GGCGCC              
KspI          141  CCGCGG               CCGC^GG
              167  CCGCGG              
MboII         951  GAAGA                GAAGA(8/7)
             1243  GAAGA               
MluNI         773  TGGCCA               TGG^CCA
              884  TGGCCA              
MscI          773  TGGCCA               TGG^CCA
              884  TGGCCA              
NarI           41  GGCGCC               GG^CGCC
              420  GGCGCC              
PvuII         682  CAGCTG               CAG^CTG
             1093  CAGCTG              
SacII         141  CCGCGG               CCGC^GG
              167  CCGCGG              
SbfI          930  CCTGCAGG             CCTGCA^GG
             1542  CCTGCAGG            
SexAI        1199  ACCTGGT              A^CC[AT]GGT
             1427  ACCAGGT             
SfoI           41  GGCGCC               GGC^GCC
              420  GGCGCC              
Sse8387I      930  CCTGCAGG             CCTGCA^GG
             1542  CCTGCAGG            
StuI          923  AGGCCT               AGG^CCT
             1547  AGGCCT              
TaqI          581  TCGA                 T^CGA
             1535  TCGA                
TspRI         569  CAGTG                CA[GC]TG[ACGT][ACGT]^
             1498  CAGTG               
TthHB8I       581  TCGA                 T^CGA
             1535  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   83 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AlwNI       AvaI        AvaII       BanI        BanII       
BbvCI       BbvI        BcnI        BmyI        BpmI        
BsaJI       BseRI       BsgI        BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BsrBI       
BsrI        BssKI       BstF5I      BstNI       BstUI       
BstXI       BstYI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraII       EaeI        EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        MaeII       
MaeIII      MboI        MflI        MnlI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NdeII       NlaIII      NlaIV       PleI        
PpuMI       PstI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        StyI        TseI        
Tsp45I      XcmI        XhoII       
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