Restriction map for pF1KB9961
 (Linear) (Six-base) MAPSORT of: KIBB9961.seq  Check: 5803  from: 1  to: 1608



KIBB9961.orf

 With 182 enzymes: SgfI * 

                        May 27, 2009 12:02  ..

Acc65I G'GTAC_C

Cuts at:      0     871    1608 
   Size:        871     737
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     919    1608 
   Size:        919     689
 
AflIII A'CryG_T

Cuts at:      0    1340    1608 
   Size:       1340     268
 
AhdI GACnn_n'nnGTC

Cuts at:      0     285    1344    1608 
   Size:        285    1059     264

  Fragments arranged by size: 

                1059     285     264
 
AleI CACnn'nnGTG

Cuts at:      0     265     736     880    1608 
   Size:        265     471     144     728

  Fragments arranged by size: 

                 728     471     265     144
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1001    1096    1608 
   Size:       1001      95     512

  Fragments arranged by size: 

                1001     512      95
 
AlwNI CAG_nnn'CTG

Cuts at:      0     574     684     771    1306    1608 
   Size:        574     110      87     535     302

  Fragments arranged by size: 

                 574     535     302     110      87
 
ApaI G_GGCC'C

Cuts at:      0     241    1608 
   Size:        241    1367
 
ApaLI G'TGCA_C

Cuts at:      0      70    1608 
   Size:         70    1538
 
ApoI r'AATT_y

Cuts at:      0    1276    1608 
   Size:       1276     332
 
AvaI C'yCGr_G

Cuts at:      0     510     797    1471    1594    1608 
   Size:        510     287     674     123      14

  Fragments arranged by size: 

                 674     510     287     123      14
 
BamHI G'GATC_C

Cuts at:      0    1355    1608 
   Size:       1355     253
 
BanI G'GyrC_C

Cuts at:      0      41     420     553     871    1270    1389    1608 
   Size:         41     379     133     318     399     119     219

  Fragments arranged by size: 

                 399     379     318     219     133     119      41
 
BanII G_rGCy'C

Cuts at:      0      59     182     241    1007    1373    1583    1608 
   Size:         59     123      59     766     366     210      25

  Fragments arranged by size: 

                 766     366     210     123      59      59      25
 
BbeI G_GCGC'C

Cuts at:      0      45     424    1608 
   Size:         45     379    1184

  Fragments arranged by size: 

                1184     379      45
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1250    1608 
   Size:       1250     358
 
BbvCI CC'TCA_GC

Cuts at:      0     443     958    1091    1608 
   Size:        443     515     133     517

  Fragments arranged by size: 

                 517     515     443     133
 
BglI GCCn_nnn'nGGC

Cuts at:      0     143     169    1608 
   Size:        143      26    1439

  Fragments arranged by size: 

                1439     143      26
 
BlpI GC'TnA_GC

Cuts at:      0     379    1608 
   Size:        379    1229
 
Bme1580I G_kGCm'C

Cuts at:      0      74     241     745    1394    1608 
   Size:         74     167     504     649     214

  Fragments arranged by size: 

                 649     504     214     167      74
 
BmgBI CAC'GTC

Cuts at:      0     749    1341    1571    1608 
   Size:        749     592     230      37

  Fragments arranged by size: 

                 749     592     230      37
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     657    1578    1608 
   Size:        657     921      30

  Fragments arranged by size: 

                 921     657      30
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      73     316     835     902    1283    1576    1608 
   Size:         73     243     519      67     381     293      32

  Fragments arranged by size: 

                 519     381     293     243      73      67      32
 
Bpu10I CC'TnA_GC

Cuts at:      0     443     958    1091    1608 
   Size:        443     515     133     517

  Fragments arranged by size: 

                 517     515     443     133
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1042    1608 
   Size:       1042     566
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     535    1608 
   Size:        535    1073
 
BsaAI yAC'GTr

Cuts at:      0     417    1137    1608 
   Size:        417     720     471

  Fragments arranged by size: 

                 720     471     417
 
BsaHI Gr'CG_yC

Cuts at:      0      42     421    1608 
   Size:         42     379    1187

  Fragments arranged by size: 

                1187     379      42
 
BsaWI w'CCGG_w

Cuts at:      0    1445    1608 
   Size:       1445     163
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     637     667     900     930    1608 
   Size:        637      30     233      30     678

  Fragments arranged by size: 

                 678     637     233      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     195     460    1015    1534    1608 
   Size:        195     265     555     519      74

  Fragments arranged by size: 

                 555     519     265     195      74
 
BseYI C'CCAG_C

Cuts at:      0     112     148     406     825    1608 
   Size:        112      36     258     419     783

  Fragments arranged by size: 

                 783     419     258     112      36
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     997    1193    1465    1608 
   Size:        997     196     272     143

  Fragments arranged by size: 

                 997     272     196     143
 
BsiEI CG_ry'CG

Cuts at:      0     131     173    1608 
   Size:        131      42    1435

  Fragments arranged by size: 

                1435     131      42
 
BsiHKAI G_wGCw'C

Cuts at:      0      74    1373    1608 
   Size:         74    1299     235

  Fragments arranged by size: 

                1299     235      74
 
BsiWI C'GTAC_G

Cuts at:      0    1133    1608 
   Size:       1133     475
 
Bsp1286I G_dGCh'C

Cuts at:      0      59      74     182     241     745    1007    1373    1394 
   Size:         59      15     108      59     504     262     366      21

Cuts at:   1394    1583    1608 
   Size:        189      25

  Fragments arranged by size: 

                 504     366     262     189     108      59      59      25
                  21      15
 
BspHI T'CATG_A

Cuts at:      0     947    1608 
   Size:        947     661
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1088    1550    1608 
   Size:       1088     462      58

  Fragments arranged by size: 

                1088     462      58
 
BsrBI CCG'CTC

Cuts at:      0     327     486    1143    1437    1524    1608 
   Size:        327     159     657     294      87      84

  Fragments arranged by size: 

                 657     327     294     159      87      84
 
BsrFI r'CCGG_y

Cuts at:      0     247    1608 
   Size:        247    1361
 
BssSI C'ACGA_G

Cuts at:      0     526    1608 
   Size:        526    1082
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     267     882    1185    1608 
   Size:        267     615     303     423

  Fragments arranged by size: 

                 615     423     303     267
 
BstYI r'GATC_y

Cuts at:      0     617     791    1355    1608 
   Size:        617     174     564     253

  Fragments arranged by size: 

                 617     564     253     174
 
Bsu36I CC'TnA_GG

Cuts at:      0    1122    1517    1608 
   Size:       1122     395      91

  Fragments arranged by size: 

                1122     395      91
 
BtgI C'CryG_G

Cuts at:      0     141     167    1608 
   Size:        141      26    1441

  Fragments arranged by size: 

                1441     141      26
 
DraIII CAC_nnn'GTG

Cuts at:      0     740    1608 
   Size:        740     868
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     306    1608 
   Size:        306    1302
 
EaeI y'GGCC_r

Cuts at:      0     128     135     170     245     773     884    1214    1317 
   Size:        128       7      35      75     528     111     330     103

Cuts at:   1317    1608 
   Size:        291

  Fragments arranged by size: 

                 528     330     291     128     111     103      75      35
                   7
 
EagI C'GGCC_G

Cuts at:      0     128     170    1608 
   Size:        128      42    1438

  Fragments arranged by size: 

                1438     128      42
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1521    1608 
   Size:       1521      87
 
EcoICRI GAG'CTC

Cuts at:      0    1371    1608 
   Size:       1371     237
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      73     316     835     902     919    1283    1576    1608 
   Size:         73     243     519      67      17     364     293      32

  Fragments arranged by size: 

                 519     364     293     243      73      67      32      17
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     775     934    1608 
   Size:        775     159     674

  Fragments arranged by size: 

                 775     674     159
 
EcoO109I rG'GnC_Cy

Cuts at:      0     518     594     768     860    1164    1608 
   Size:        518      76     174      92     304     444

  Fragments arranged by size: 

                 518     444     304     174      92      76
 
HaeII r_GCGC'y

Cuts at:      0      45      80      92     424    1608 
   Size:         45      35      12     332    1184

  Fragments arranged by size: 

                1184     332      45      35      12
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     271     303    1330    1362    1608 
   Size:        271      32    1027      32     246

  Fragments arranged by size: 

                1027     271     246      32      32
 
HincII GTy'rAC

Cuts at:      0     342    1608 
   Size:        342    1266
 
KasI G'GCGC_C

Cuts at:      0      41     420    1608 
   Size:         41     379    1188

  Fragments arranged by size: 

                1188     379      41
 
KpnI G_GTAC'C

Cuts at:      0     875    1608 
   Size:        875     733
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     452    1316    1608 
   Size:        452     864     292

  Fragments arranged by size: 

                 864     452     292
 
MscI TGG'CCA

Cuts at:      0     775     886    1608 
   Size:        775     111     722

  Fragments arranged by size: 

                 775     722     111
 
MslI CAynn'nnrTG

Cuts at:      0     265     710     736     880    1186    1608 
   Size:        265     445      26     144     306     422

  Fragments arranged by size: 

                 445     422     306     265     144      26
 
MspA1I CmG'CkG

Cuts at:      0      39     143     169     243     635     684    1095    1608 
   Size:         39     104      26      74     392      49     411     513

  Fragments arranged by size: 

                 513     411     392     104      74      49      39      26
            
 
NarI GG'CG_CC

Cuts at:      0      42     421    1608 
   Size:         42     379    1187

  Fragments arranged by size: 

                1187     379      42
 
NotI GC'GGCC_GC

Cuts at:      0     170    1608 
   Size:        170    1438
 
PasI CC'CwG_GG

Cuts at:      0    1399    1608 
   Size:       1399     209
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1289    1608 
   Size:       1289     319
 
PfoI T'CCnGG_A

Cuts at:      0    1553    1608 
   Size:       1553      55
 
PpuMI rG'GwC_Cy

Cuts at:      0     518     860    1164    1608 
   Size:        518     342     304     444

  Fragments arranged by size: 

                 518     444     342     304
 
PshAI GACnn'nnGTC

Cuts at:      0     477    1608 
   Size:        477    1131
 
PspOMI G'GGCC_C

Cuts at:      0     237    1608 
   Size:        237    1371
 
PstI C_TGCA'G

Cuts at:      0     680     935    1547    1608 
   Size:        680     255     612      61

  Fragments arranged by size: 

                 680     612     255      61
 
PvuII CAG'CTG

Cuts at:      0     684    1095    1608 
   Size:        684     411     513

  Fragments arranged by size: 

                 684     513     411
 
SacI G_AGCT'C

Cuts at:      0    1373    1608 
   Size:       1373     235
 
SacII CC_GC'GG

Cuts at:      0     144     170    1608 
   Size:        144      26    1438

  Fragments arranged by size: 

                1438     144      26
 
SbfI CC_TGCA'GG

Cuts at:      0     935    1547    1608 
   Size:        935     612      61

  Fragments arranged by size: 

                 935     612      61
 
SexAI A'CCwGG_T

Cuts at:      0    1199    1427    1608 
   Size:       1199     228     181

  Fragments arranged by size: 

                1199     228     181
 
SfcI C'TryA_G

Cuts at:      0     676     931    1543    1608 
   Size:        676     255     612      65

  Fragments arranged by size: 

                 676     612     255      65
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     143    1608 
   Size:        143    1465
 
SfoI GGC'GCC

Cuts at:      0      43     422    1608 
   Size:         43     379    1186

  Fragments arranged by size: 

                1186     379      43
 
SmaI CCC'GGG

Cuts at:      0    1596    1608 
   Size:       1596      12
 
SmlI C'TyrA_G

Cuts at:      0    1057    1608 
   Size:       1057     551
 
StuI AGG'CCT

Cuts at:      0     925    1549    1608 
   Size:        925     624      59

  Fragments arranged by size: 

                 925     624      59
 
StyI C'CwwG_G

Cuts at:      0      23     590     776    1608 
   Size:         23     567     186     832

  Fragments arranged by size: 

                 832     567     186      23
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     676    1608 
   Size:        676     932
 
TatI w'GTAC_w

Cuts at:      0    1025    1253    1608 
   Size:       1025     228     355

  Fragments arranged by size: 

                1025     355     228
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     900     932    1608 
   Size:        900      32     676

  Fragments arranged by size: 

                 900     676      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     214    1608 
   Size:        214    1394
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     414     838    1185    1608 
   Size:        414     424     347     423

  Fragments arranged by size: 

                 424     423     414     347
 
XmaI C'CCGG_G

Cuts at:      0    1594    1608 
   Size:       1594      14
 

 Enzymes that do cut:

   Acc65I     AcuI   AflIII     AhdI     AleI     AlfI    AlwNI     ApaI
    ApaLI     ApoI     AvaI    BamHI     BanI    BanII     BbeI     BbsI
    BbvCI     BglI     BlpI Bme1580I    BmgBI     BmrI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI Bsp1286I    BspHI    BspMI    BsrBI
    BsrFI    BssSI    BstXI    BstYI   Bsu36I     BtgI   DraIII     DrdI
     EaeI     EagI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    HaeII
    Hin4I   HincII     KasI     KpnI     MmeI     MscI     MslI   MspA1I
     NarI     NotI     PasI    PflMI     PfoI    PpuMI    PshAI   PspOMI
     PstI    PvuII     SacI    SacII     SbfI    SexAI     SfcI     SfiI
     SfoI     SmaI     SmlI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII     AgeI     AloI
     AscI     AseI    AsiSI    AvrII     BaeI     BcgI    BciVI     BclI
    BfrBI    BglII     BmtI     BplI    BsaBI     BsmI    BsmBI    BspEI
    BsrDI    BsrGI   BssHII   BstAPI    BstBI   BstEII  BstZ17I    BtgZI
     BtsI     ClaI    CspCI     DraI     EarI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI  HindIII     HpaI     MfeI     MluI     NaeI
     NcoI     NdeI   NgoMIV     NheI     NruI     NsiI     NspI     PacI
     PciI     PmeI     PmlI     PpiI     PsiI    PspXI     PsrI     PvuI
    RsrII     SalI    SanDI     SapI     ScaI     SgfI    SgrAI    SnaBI
     SpeI     SphI     SrfI     SspI     SwaI     XbaI     XhoI     XmnI
     ZraI
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