Restriction map for pF1KE1076
##################################
# NO CUT ENZYMES (  164 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AflII       AflIII      AgeI        Alw44I      
AlwNI       Aor51HI     ApaLI       AscI        AseI        
AsnI        Asp700I     Asp718I     AspI        AvaI        
AviII       AvrII       BamHI       BbeI        BbrPI       
BbvCI       BcgI        BciVI       BclI        BcnI        
BfrI        BglI        BglII       BlnI        BlpI        
Bpu1102I    BsaAI       BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BsgI        BsiEI       BsiWI       
BsmBI       BsmI        BsoBI       BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrFI       BssHII      
BssKI       Bst1107I    BstAPI      BstBI       BstNI       
BstZ17I     CelII       CfoI        Cfr10I      ClaI        
CpoI        DraI        DraIII      EagI        EarI        
EclXI       Eco47III    Eco52I      EcoNI       EcoRI       
EcoRII      EcoRV       EcoT22I     FbaI        FseI        
FspI        HaeII       HapII       HhaI        Hin1I       
HinP1I      HincII      HindII      HindIII     HinfI       
HpaI        HpaII       KasI        KpnI        Ksp632I     
KspI        MamI        MluI        MroI        MspI        
MvaI        NaeI        NarI        NciI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PleI        PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        PvuII       RcaI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScrFI       SexAI       SfaNI       SfcI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspI        StuI        SwaI        TaqI        TfiI        
Tth111I     TthHB8I     Van91I      XbaI        XcmI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AhdI          716  GACGGGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AluI          776  AGCT                 AG^CT
AocI          823  CCTGAGG              CC^T[ACGT]AGG
ApaI          399  GGGCCC               GGGCC^C
AspEI         716  GACGGGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI         624  GAGCAC               G[AT]GC[AT]^C
AvaII         731  GGACC                G^G[AT]CC
BalI          296  TGGCCA               TGG^CCA
BanII         399  GGGCCC               G[GA]GC[CT]^C
BbsI          160  GAAGAC               GAAGAC(2/6)
BfaI          448  CTAG                 C^TAG
BpuAI         160  GAAGAC               GAAGAC(2/6)
BsiHKAI       624  GAGCAC               G[AT]GC[AT]^C
BsmFI         455  GGGAC                GGGAC(10/14)
Bsp1407I      556  TGTACA               T^GTACA
BsrBI         130  CCGCTC               CCGCTC(-3/-3)
BsrDI         685  GCAATG               GCAATG(2/0)
BsrGI         556  TGTACA               T^GTACA
BsrI          185  ACTGG                ACTGG(1/-1)
BstEII        166  GGTAACC              G^GT[ACGT]ACC
BstF5I        582  GGATG                GGATG(2/0)
BstPI         166  GGTAACC              G^GT[ACGT]ACC
BstXI         248  CCATCACTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        823  CCTGAGG              CC^T[ACGT]AGG
DraII         399  GGGCCCT              [GA]G^G[ACGT]CC[CT]
DrdI          457  GACAAGAGGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI          138  CCACGG               C^C[GA][CT]GG
Eam1105I      716  GACGGGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I        823  CCTGAGG              CC^T[ACGT]AGG
EcoO109I      399  GGGCCCT              [GA]G^G[ACGT]CC[CT]
EcoO65I       166  GGTAACC              G^GT[ACGT]ACC
EcoT14I       669  CCAAGG               C^C[AT][AT]GG
FauI          786  GCGGG                CCCGC(4/6)
FokI          582  GGATG                GGATG(9/13)
HgaI          590  GACGC                GACGC(5/10)
MaeI          448  CTAG                 C^TAG
MaeII         376  ACGT                 A^CGT
MfeI          492  CAATTG               C^AATTG
MluNI         296  TGGCCA               TGG^CCA
MscI          296  TGGCCA               TGG^CCA
MspA1I        784  CAGCGG               C[AC]G^C[GT]G
MunI          492  CAATTG               C^AATTG
ScaI          416  AGTACT               AGT^ACT
SspBI         556  TGTACA               T^GTACA
StyI          669  CCAAGG               C^C[AT][AT]GG


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AcsI          567  AAATTT               [GA]^AATT[CT]
              760  GAATTT              
ApoI          567  AAATTT               [GA]^AATT[CT]
              760  GAATTT              
BanI           12  GGCACC               G^G[CT][GA]CC
               39  GGTGCC              
BbvI          831  GCAGC                GCAGC(8/12)
              100  GCTGC               
BmyI          399  GGGCCC               G[AGT]GC[ACT]^C
              624  GAGCAC              
BpmI          186  CTGGAG               CTGGAG(16/14)
              809  CTGGAG              
BsaJI         138  CCACGG               C^C[ACGT][ACGT]GG
              669  CCAAGG              
BseRI         742  GAGGAG               GAGGAG(10/8)
              826  GAGGAG              
BsiYI          78  CCTTCTGTCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              390  CCTATATATGG         
BslI           78  CCTTCTGTCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              390  CCTATATATGG         
BsmAI         812  GAGAC                GTCTC(1/5)
              724  GTCTC               
Bsp1286I      399  GGGCCC               G[AGT]GC[ACT]^C
              624  GAGCAC              
BssSI          28  CTCGTG               CACGAG(-5/-1)
              112  CTCGTG              
BstYI         200  AGATCC               [GA]^GATC[CT]
              244  AGATCC              
Cac8I          60  GCTCGC               GC[ACGT]^[ACGT]GC
              786  GCGGGC              
EaeI          296  TGGCCA               [CT]^GGCC[GA]
              694  CGGCCA              
HphI          631  TCACC                GGTGA(8/7)
              638  TCACC               
MflI          200  AGATCC               [GA]^GATC[CT]
              244  AGATCC              
MwoI            4  GCGGTCTTGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              777  GCTATACCAGC         
TseI          100  GCTGC                G^C[AT]GC
              831  GCAGC               
Tsp45I        176  GTGAC                ^GT[GC]AC
              466  GTCAC               
XhoII         200  AGATCC               [GA]^GATC[CT]
              244  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   27 enzymes) #
#########################################

AccII       AciI        AfaI        AlwI        BstUI       
Cfr13I      DdeI        DpnII       Fnu4HI      HaeIII      
ItaI        MaeIII      MboI        MboII       MnlI        
MseI        MslI        MvnI        NdeII       NlaIII      
NlaIV       RsaI        Sau3AI      Sau96I      Tru9I       
Tsp509I     TspRI       
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