Restriction map for pF1KE1076
 (Linear) (Six-base) MAPSORT of: KIEE1076.seq  Check: 5612  from: 1  to: 888



KIEE1076.orf

 With 182 enzymes: SgfI * 

                        February 15, 2010 17:50  ..

AhdI GACnn_n'nnGTC

Cuts at:      0     721     888 
   Size:        721     167
 
AleI CACnn'nnGTG

Cuts at:      0     856     888 
   Size:        856      32
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     229     261     888 
   Size:        229      32     627

  Fragments arranged by size: 

                 627     229      32
 
ApaI G_GGCC'C

Cuts at:      0     403     888 
   Size:        403     485
 
ApoI r'AATT_y

Cuts at:      0     567     760     888 
   Size:        567     193     128

  Fragments arranged by size: 

                 567     193     128
 
BanI G'GyrC_C

Cuts at:      0      12      39     888 
   Size:         12      27     849

  Fragments arranged by size: 

                 849      27      12
 
BanII G_rGCy'C

Cuts at:      0     403     888 
   Size:        403     485
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     167     888 
   Size:        167     721
 
Bme1580I G_kGCm'C

Cuts at:      0     403     888 
   Size:        403     485
 
BmgBI CAC'GTC

Cuts at:      0     377     888 
   Size:        377     511
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     207     830     888 
   Size:        207     623      58

  Fragments arranged by size: 

                 623     207      58
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     757     841     888 
   Size:        757      84      47

  Fragments arranged by size: 

                 757      84      47
 
BsiHKAI G_wGCw'C

Cuts at:      0     628     888 
   Size:        628     260
 
Bsp1286I G_dGCh'C

Cuts at:      0     403     628     888 
   Size:        403     225     260

  Fragments arranged by size: 

                 403     260     225
 
BsrBI CCG'CTC

Cuts at:      0     132     888 
   Size:        132     756
 
BsrDI GCAATG_nn'

Cuts at:      0     692     888 
   Size:        692     196
 
BsrGI T'GTAC_A

Cuts at:      0     556     888 
   Size:        556     332
 
BssSI C'ACGA_G

Cuts at:      0      28     112     888 
   Size:         28      84     776

  Fragments arranged by size: 

                 776      84      28
 
BstEII G'GTnAC_C

Cuts at:      0     166     888 
   Size:        166     722
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     255     888 
   Size:        255     633
 
BstYI r'GATC_y

Cuts at:      0     200     244     888 
   Size:        200      44     644

  Fragments arranged by size: 

                 644     200      44
 
Bsu36I CC'TnA_GG

Cuts at:      0     824     888 
   Size:        824      64
 
BtgI C'CryG_G

Cuts at:      0     138     888 
   Size:        138     750
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     463     888 
   Size:        463     425
 
EaeI y'GGCC_r

Cuts at:      0     296     694     888 
   Size:        296     398     194

  Fragments arranged by size: 

                 398     296     194
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     207     830     888 
   Size:        207     623      58

  Fragments arranged by size: 

                 623     207      58
 
EcoO109I rG'GnC_Cy

Cuts at:      0     400     888 
   Size:        400     488
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     496     528     707     739     803     835     888 
   Size:        496      32     179      32      64      32      53

  Fragments arranged by size: 

                 496     179      64      53      32      32      32
 
MfeI C'AATT_G

Cuts at:      0     492     888 
   Size:        492     396
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     567     888 
   Size:        567     321
 
MscI TGG'CCA

Cuts at:      0     298     888 
   Size:        298     590
 
MslI CAynn'nnrTG

Cuts at:      0     226     253     280     856     888 
   Size:        226      27      27     576      32

  Fragments arranged by size: 

                 576     226      32      27      27
 
MspA1I CmG'CkG

Cuts at:      0     786     888 
   Size:        786     102
 
PspOMI G'GGCC_C

Cuts at:      0     399     888 
   Size:        399     489
 
ScaI AGT'ACT

Cuts at:      0     418     888 
   Size:        418     470
 
StyI C'CwwG_G

Cuts at:      0     669     888 
   Size:        669     219
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     166     888 
   Size:        166     722
 
TatI w'GTAC_w

Cuts at:      0     416     556     888 
   Size:        416     140     332

  Fragments arranged by size: 

                 416     332     140
 

 Enzymes that do cut:

     AhdI     AleI     AloI     ApaI     ApoI     BanI    BanII     BbsI
 Bme1580I    BmgBI     BpmI    BseRI  BsiHKAI Bsp1286I    BsrBI    BsrDI
    BsrGI    BssSI   BstEII    BstXI    BstYI   Bsu36I     BtgI     DrdI
     EaeI  Eco57MI EcoO109I    Hin4I     MfeI     MmeI     MscI     MslI
   MspA1I   PspOMI     ScaI     StyI    TaqII     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AlfI    AlwNI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BbeI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI     BmrI     BmtI     BplI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BspMI    BsrFI   BssHII   BstAPI    BstBI  BstZ17I    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     EagI     EarI     EciI  EcoICRI
    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
   HincII  HindIII     HpaI     KasI     KpnI     MluI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI
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