Restriction map for pF1KB3024
 (Linear) (Six-base) MAPSORT of: KIBB3024.seq  Check: 7486  from: 1  to: 4056



KIBB3024.orf

 With 182 enzymes: SgfI * 

                        December 26, 2011 13:17  ..

AatII G_ACGT'C

Cuts at:      0    2248    4056 
   Size:       2248    1808
 
AccI GT'mk_AC

Cuts at:      0    1156    2492    3143    4056 
   Size:       1156    1336     651     913

  Fragments arranged by size: 

                1336    1156     913     651
 
Acc65I G'GTAC_C

Cuts at:      0    3363    4056 
   Size:       3363     693
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    3509    3912    4056 
   Size:       3509     403     144

  Fragments arranged by size: 

                3509     403     144
 
AfeI AGC'GCT

Cuts at:      0    3244    4056 
   Size:       3244     812
 
AflIII A'CryG_T

Cuts at:      0    1121    4056 
   Size:       1121    2935
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1184    4056 
   Size:       1184    2872
 
AleI CACnn'nnGTG

Cuts at:      0     361    1278    2189    2996    4056 
   Size:        361     917     911     807    1060

  Fragments arranged by size: 

                1060     917     911     807     361
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1028    4056 
   Size:       1028    3028
 
AlwNI CAG_nnn'CTG

Cuts at:      0     585     933    1572    1967    2754    3394    4056 
   Size:        585     348     639     395     787     640     662

  Fragments arranged by size: 

                 787     662     640     639     585     395     348
 
ApaI G_GGCC'C

Cuts at:      0     184    1030    1865    3277    3757    4056 
   Size:        184     846     835    1412     480     299

  Fragments arranged by size: 

                1412     846     835     480     299     184
 
ApaLI G'TGCA_C

Cuts at:      0     366    1720    4056 
   Size:        366    1354    2336

  Fragments arranged by size: 

                2336    1354     366
 
ApoI r'AATT_y

Cuts at:      0    1592    2531    3214    4056 
   Size:       1592     939     683     842

  Fragments arranged by size: 

                1592     939     842     683
 
AvaI C'yCGr_G

Cuts at:      0     152     406     857    1144    1307    1838    3074    3399 
   Size:        152     254     451     287     163     531    1236     325

Cuts at:   3399    3746    3871    4056 
   Size:        347     125     185

  Fragments arranged by size: 

                1236     531     451     347     325     287     254     185
                 163     152     125
 
BamHI G'GATC_C

Cuts at:      0    1216    2306    2429    2507    3065    4056 
   Size:       1216    1090     123      78     558     991

  Fragments arranged by size: 

                1216    1090     991     558     123      78
 
BanI G'GyrC_C

Cuts at:      0     601    1165    1606    1728    2940    3363    3428    3516 
   Size:        601     564     441     122    1212     423      65      88

Cuts at:   3516    3781    3856    4056 
   Size:        265      75     200

  Fragments arranged by size: 

                1212     601     564     441     423     265     200     122
                  88      75      65
 
BanII G_rGCy'C

Cuts at:      0     150     184     323     895    1030    1865    1940    2626 
   Size:        150      34     139     572     135     835      75     686

Cuts at:   2626    2746    3277    3485    3757    3987    4056 
   Size:        120     531     208     272     230      69

  Fragments arranged by size: 

                 835     686     572     531     272     230     208     150
                 139     135     120      75      69      34
 
BbeI G_GCGC'C

Cuts at:      0    1169    1610    4056 
   Size:       1169     441    2446

  Fragments arranged by size: 

                2446    1169     441
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1554    4056 
   Size:       1554    2502
 
BbvCI CC'TCA_GC

Cuts at:      0    1555    2449    3350    3905    4056 
   Size:       1555     894     901     555     151

  Fragments arranged by size: 

                1555     901     894     555     151
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1829    1863    4056 
   Size:       1829      34    2193

  Fragments arranged by size: 

                2193    1829      34
 
BclI T'GATC_A

Cuts at:      0    3149    4056 
   Size:       3149     907
 
BglI GCCn_nnn'nGGC

Cuts at:      0     154    2648    4056 
   Size:        154    2494    1408

  Fragments arranged by size: 

                2494    1408     154
 
BglII A'GATC_T

Cuts at:      0     731    1397    4056 
   Size:        731     666    2659

  Fragments arranged by size: 

                2659     731     666
 
Bme1580I G_kGCm'C

Cuts at:      0     184     370     966    1030    1308    1724    1733    1847 
   Size:        184     186     596      64     278     416       9     114

Cuts at:   1847    1865    2055    2945    3277    3757    4056 
   Size:         18     190     890     332     480     299

  Fragments arranged by size: 

                 890     596     480     416     332     299     278     190
                 186     184     114      64      18       9
 
BmgBI CAC'GTC

Cuts at:      0    1212    4047    4056 
   Size:       1212    2835       9

  Fragments arranged by size: 

                2835    1212       9
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1681    1863    3966    4056 
   Size:       1681     182    2103      90

  Fragments arranged by size: 

                2103    1681     182      90
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1450    2818    2983    3337    3499    4056 
   Size:       1450    1368     165     354     162     557

  Fragments arranged by size: 

                1450    1368     557     354     165     162
 
Bpu10I CC'TnA_GC

Cuts at:      0    1555    2449    3350    3905    4056 
   Size:       1555     894     901     555     151

  Fragments arranged by size: 

                1555     901     894     555     151
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      99    4056 
   Size:         99    3957
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     403     644    3512    4056 
   Size:        403     241    2868     544

  Fragments arranged by size: 

                2868     544     403     241
 
BsaAI yAC'GTr

Cuts at:      0     540    2607    4056 
   Size:        540    2067    1449

  Fragments arranged by size: 

                2067    1449     540
 
BsaBI GATnn'nnATC

Cuts at:      0    2311    2428    4056 
   Size:       2311     117    1628

  Fragments arranged by size: 

                2311    1628     117
 
BsaHI Gr'CG_yC

Cuts at:      0    1166    1607    2245    2736    4056 
   Size:       1166     441     638     491    1320

  Fragments arranged by size: 

                1320    1166     638     491     441
 
BsaWI w'CCGG_w

Cuts at:      0     130    2025    2336    4056 
   Size:        130    1895     311    1720

  Fragments arranged by size: 

                1895    1720     311     130
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     266     296    1432    1462    3000    3030    3193    3223 
   Size:        266      30    1136      30    1538      30     163      30

Cuts at:   3223    3441    3471    4056 
   Size:        218      30     585

  Fragments arranged by size: 

                1538    1136     585     266     218     163      30      30
                  30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      70      79      82     313     316    2161    3367    3508 
   Size:         70       9       3     231       3    1845    1206     141

Cuts at:   3508    3908    4056 
   Size:        400     148

  Fragments arranged by size: 

                1845    1206     400     231     148     141      70       9
                   3       3
 
BseYI C'CCAG_C

Cuts at:      0      20     243     314     605     767     902    1095    1443 
   Size:         20     223      71     291     162     135     193     348

Cuts at:   1443    1632    1647    1850    2092    3069    3116    3239    3276 
   Size:        189      15     203     242     977      47     123      37

Cuts at:   3276    3368    3406    3607    3695    3731    3933    3991    4056 
   Size:         92      38     201      88      36     202      58      65

  Fragments arranged by size: 

                 977     348     291     242     223     203     202     201
                 193     189     162     135     123      92      88      71
                  65      58      47      38      37      36      20      15
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     478     550    1005    1072    1206    1225    1311    1582 
   Size:        478      72     455      67     134      19      86     271

Cuts at:   1582    2163    2692    3114    4056 
   Size:        581     529     422     942

  Fragments arranged by size: 

                 942     581     529     478     455     422     271     134
                  86      72      67      19
 
BsiHKAI G_wGCw'C

Cuts at:      0     289     358     370     545    1337    1724    4056 
   Size:        289      69      12     175     792     387    2332

  Fragments arranged by size: 

                2332     792     387     289     175      69      12
 
BsmI GAATG_Cn'

Cuts at:      0    1924    1962    2270    4056 
   Size:       1924      38     308    1786

  Fragments arranged by size: 

                1924    1786     308      38
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    2434    4056 
   Size:       2434    1622
 
Bsp1286I G_dGCh'C

Cuts at:      0     150     184     289     323     358     370     545     895 
   Size:        150      34     105      34      35      12     175     350

Cuts at:    895     966    1030    1308    1337    1724    1733    1847    1865 
   Size:         71      64     278      29     387       9     114      18

Cuts at:   1865    1940    2055    2626    2746    2945    3277    3485    3757 
   Size:         75     115     571     120     199     332     208     272

Cuts at:   3757    3987    4056 
   Size:        230      69

  Fragments arranged by size: 

                 571     387     350     332     278     272     230     208
                 199     175     150     120     115     114     105      75
                  71      69      64      35      34      34      29      18
                  12       9
 
BspEI T'CCGG_A

Cuts at:      0    2336    4056 
   Size:       2336    1720
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     491     558    1580    2762    3098    3694    3783    4056 
   Size:        491      67    1022    1182     336     596      89     273

  Fragments arranged by size: 

                1182    1022     596     491     336     273      89      67
            
 
BsrBI CCG'CTC

Cuts at:      0     854    1464    2833    3108    3308    3978    4056 
   Size:        854     610    1369     275     200     670      78

  Fragments arranged by size: 

                1369     854     670     610     275     200      78
 
BsrFI r'CCGG_y

Cuts at:      0     370     952    1015    1151    1784    2978    4056 
   Size:        370     582      63     136     633    1194    1078

  Fragments arranged by size: 

                1194    1078     633     582     370     136      63
 
BssHII G'CGCG_C

Cuts at:      0      27     163     197    4056 
   Size:         27     136      34    3859

  Fragments arranged by size: 

                3859     136      34      27
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     585    1728    1971    4056 
   Size:        585    1143     243    2085

  Fragments arranged by size: 

                2085    1143     585     243
 
BstBI TT'CG_AA

Cuts at:      0     717    4056 
   Size:        717    3339
 
BstEII G'GTnAC_C

Cuts at:      0    1104    1513    3553    4056 
   Size:       1104     409    2040     503

  Fragments arranged by size: 

                2040    1104     503     409
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     297    2929    3288    4056 
   Size:        297    2632     359     768

  Fragments arranged by size: 

                2632     768     359     297
 
BstYI r'GATC_y

Cuts at:      0     731    1216    1397    2306    2333    2429    2470    2507 
   Size:        731     485     181     909      27      96      41      37

Cuts at:   2507    3065    3080    4056 
   Size:        558      15     976

  Fragments arranged by size: 

                 976     909     731     558     485     181      96      41
                  37      27      15
 
BstZ17I GTA'TAC

Cuts at:      0    1157    4056 
   Size:       1157    2899
 
BtgI C'CryG_G

Cuts at:      0     175     264     290    2666    2730    3860    4056 
   Size:        175      89      26    2376      64    1130     196

  Fragments arranged by size: 

                2376    1130     196     175      89      64      26
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     531    2419    4056 
   Size:        531    1888    1637

  Fragments arranged by size: 

                1888    1637     531
 
BtsI GCAGTG_nn'

Cuts at:      0     287     574     979    1012    1089    1454    1683    1775 
   Size:        287     287     405      33      77     365     229      92

Cuts at:   1775    1845    3310    4056 
   Size:         70    1465     746

  Fragments arranged by size: 

                1465     746     405     365     287     287     229      92
                  77      70      33
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1053    1088    4056 
   Size:       1053      35    2968

  Fragments arranged by size: 

                2968    1053      35
 
DraIII CAC_nnn'GTG

Cuts at:      0    1728    1871    4056 
   Size:       1728     143    2185

  Fragments arranged by size: 

                2185    1728     143
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1558    4056 
   Size:       1558    2498
 
EaeI y'GGCC_r

Cuts at:      0     632    1534    1786    2540    2828    3187    3227    4056 
   Size:        632     902     252     754     288     359      40     829

  Fragments arranged by size: 

                 902     829     754     632     359     288     252      40
            
 
EarI CTCTTCn'nnn_

Cuts at:      0    1496    3485    4056 
   Size:       1496    1989     571

  Fragments arranged by size: 

                1989    1496     571
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     418    2965    4056 
   Size:        418    2547    1091

  Fragments arranged by size: 

                2547    1091     418
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1450    2818    2983    3337    3499    3509    3912    4056 
   Size:       1450    1368     165     354     162      10     403     144

  Fragments arranged by size: 

                1450    1368     403     354     165     162     144      10
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     439     677    4056 
   Size:        439     238    3379

  Fragments arranged by size: 

                3379     439     238
 
EcoO109I rG'GnC_Cy

Cuts at:      0     180     181     570     683    1027    1440    1812    1861 
   Size:        180       1     389     113     344     413     372      49

Cuts at:   1861    2064    2751    3234    3336    3462    3498    3633    3753 
   Size:        203     687     483     102     126      36     135     120

Cuts at:   3753    3754    3966    4056 
   Size:          1     212      90

  Fragments arranged by size: 

                 687     483     413     389     372     344     212     203
                 180     135     126     120     113     102      90      49
                  36       1       1
 
EcoRI G'AATT_C

Cuts at:      0    1592    3214    4056 
   Size:       1592    1622     842

  Fragments arranged by size: 

                1622    1592     842
 
EcoRV GAT'ATC

Cuts at:      0     519    4056 
   Size:        519    3537
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     822    1416    4056 
   Size:        822     594    2640

  Fragments arranged by size: 

                2640     822     594
 
HaeII r_GCGC'y

Cuts at:      0    1169    1610    2480    2962    3246    3407    4056 
   Size:       1169     441     870     482     284     161     649

  Fragments arranged by size: 

                1169     870     649     482     441     284     161
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1170    1201    1202    1233    1432    1464    1673    1705 
   Size:       1170      31       1      31     199      32     209      32

Cuts at:   1705    3193    3225    4056 
   Size:       1488      32     831

  Fragments arranged by size: 

                1488    1170     831     209     199      32      32      32
                  31      31       1
 
HincII GTy'rAC

Cuts at:      0    1878    4056 
   Size:       1878    2178
 
KasI G'GCGC_C

Cuts at:      0    1165    1606    4056 
   Size:       1165     441    2450

  Fragments arranged by size: 

                2450    1165     441
 
KpnI G_GTAC'C

Cuts at:      0    3367    4056 
   Size:       3367     689
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1475    3145    4056 
   Size:       1475    1670     911

  Fragments arranged by size: 

                1670    1475     911
 
MscI TGG'CCA

Cuts at:      0    2542    3229    4056 
   Size:       2542     687     827

  Fragments arranged by size: 

                2542     827     687
 
MslI CAynn'nnrTG

Cuts at:      0     361     379    1278    2189    2548    2927    2996    3151 
   Size:        361      18     899     911     359     379      69     155

Cuts at:   3151    4056 
   Size:        905

  Fragments arranged by size: 

                 911     905     899     379     361     359     155      69
                  18
 
MspA1I CmG'CkG

Cuts at:      0     314     657     759     825     899     968    1172    1278 
   Size:        314     343     102      66      74      69     204     106

Cuts at:   1278    1643    1830    2087    2096    2388    2676    2732    3621 
   Size:        365     187     257       9     292     288      56     889

Cuts at:   3621    4056 
   Size:        435

  Fragments arranged by size: 

                 889     435     365     343     314     292     288     257
                 204     187     106     102      74      69      66      56
                   9
 
NaeI GCC'GGC

Cuts at:      0    1017    2980    4056 
   Size:       1017    1963    1076

  Fragments arranged by size: 

                1963    1076    1017
 
NarI GG'CG_CC

Cuts at:      0    1166    1607    4056 
   Size:       1166     441    2449

  Fragments arranged by size: 

                2449    1166     441
 
NcoI C'CATG_G

Cuts at:      0     264     290    4056 
   Size:        264      26    3766

  Fragments arranged by size: 

                3766     264      26
 
NdeI CA'TA_TG

Cuts at:      0    2631    4056 
   Size:       2631    1425
 
NgoMIV G'CCGG_C

Cuts at:      0    1015    2978    4056 
   Size:       1015    1963    1078

  Fragments arranged by size: 

                1963    1078    1015
 
NspI r_CATG'y

Cuts at:      0     426    2124    2159    4056 
   Size:        426    1698      35    1897

  Fragments arranged by size: 

                1897    1698     426      35
 
PasI CC'CwG_GG

Cuts at:      0    1733    1943    3814    3888    4056 
   Size:       1733     210    1871      74     168

  Fragments arranged by size: 

                1871    1733     210     168      74
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2043    2951    4056 
   Size:       2043     908    1105

  Fragments arranged by size: 

                2043    1105     908
 
PfoI T'CCnGG_A

Cuts at:      0     521    1373    2720    3197    4056 
   Size:        521     852    1347     477     859

  Fragments arranged by size: 

                1347     859     852     521     477
 
PpuMI rG'GwC_Cy

Cuts at:      0     570    1440    2064    2751    3234    3336    3462    3633 
   Size:        570     870     624     687     483     102     126     171

Cuts at:   3633    3966    4056 
   Size:        333      90

  Fragments arranged by size: 

                 870     687     624     570     483     333     171     126
                 102      90
 
PspOMI G'GGCC_C

Cuts at:      0     180    1026    1861    3273    3753    4056 
   Size:        180     846     835    1412     480     303

  Fragments arranged by size: 

                1412     846     835     480     303     180
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    3859    3891    4015    4047    4056 
   Size:       3859      32     124      32       9

  Fragments arranged by size: 

                3859     124      32      32       9
 
PstI C_TGCA'G

Cuts at:      0     515     581     713    1885    1934    2366    3691    4056 
   Size:        515      66     132    1172      49     432    1325     365

  Fragments arranged by size: 

                1325    1172     515     432     365     132      66      49
            
 
PvuII CAG'CTG

Cuts at:      0     825    1172    1643    1830    2676    4056 
   Size:        825     347     471     187     846    1380

  Fragments arranged by size: 

                1380     846     825     471     347     187
 
SacII CC_GC'GG

Cuts at:      0    2733    4056 
   Size:       2733    1323
 
SanDI GG'GwC_CC

Cuts at:      0    1440    3234    4056 
   Size:       1440    1794     822

  Fragments arranged by size: 

                1794    1440     822
 
SbfI CC_TGCA'GG

Cuts at:      0     515    4056 
   Size:        515    3541
 
ScaI AGT'ACT

Cuts at:      0    1928    3877    4056 
   Size:       1928    1949     179

  Fragments arranged by size: 

                1949    1928     179
 
SexAI A'CCwGG_T

Cuts at:      0    3338    4056 
   Size:       3338     718
 
SfcI C'TryA_G

Cuts at:      0     511     577     709    1881    1930    2362    3616    3687 
   Size:        511      66     132    1172      49     432    1254      71

Cuts at:   3687    4056 
   Size:        369

  Fragments arranged by size: 

                1254    1172     511     432     369     132      71      66
                  49
 
SfoI GGC'GCC

Cuts at:      0    1167    1608    4056 
   Size:       1167     441    2448

  Fragments arranged by size: 

                2448    1167     441
 
SgrAI Cr'CCGG_yG

Cuts at:      0    2978    4056 
   Size:       2978    1078
 
SmaI CCC'GGG

Cuts at:      0     154    3076    3401    4056 
   Size:        154    2922     325     655

  Fragments arranged by size: 

                2922     655     325     154
 
SmlI C'TyrA_G

Cuts at:      0      78    4056 
   Size:         78    3978
 
SphI G_CATG'C

Cuts at:      0    2159    4056 
   Size:       2159    1897
 
StuI AGG'CCT

Cuts at:      0     698    4056 
   Size:        698    3358
 
StyI C'CwwG_G

Cuts at:      0     264     290    2780    3287    3575    4056 
   Size:        264      26    2490     507     288     481

  Fragments arranged by size: 

                2490     507     481     288     264      26
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     373     619    1720    1882    2197    3004    4005    4056 
   Size:        373     246    1101     162     315     807    1001      51

  Fragments arranged by size: 

                1101    1001     807     373     315     246     162      51
            
 
TatI w'GTAC_w

Cuts at:      0    1926    2342    3875    4056 
   Size:       1926     416    1533     181

  Fragments arranged by size: 

                1926    1533     416     181
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     266     298    3000    3032    3441    3473    4056 
   Size:        266      32    2702      32     409      32     583

  Fragments arranged by size: 

                2702     583     409     266      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    2716    4056 
   Size:       2716    1340
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1381    2673    2952    3510    4056 
   Size:       1381    1292     279     558     546

  Fragments arranged by size: 

                1381    1292     558     546     279
 
XmaI C'CCGG_G

Cuts at:      0     152    3074    3399    4056 
   Size:        152    2922     325     657

  Fragments arranged by size: 

                2922     657     325     152
 
ZraI GAC'GTC

Cuts at:      0    2246    4056 
   Size:       2246    1810
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AcuI     AfeI   AflIII     AhdI     AleI
     AlfI    AlwNI     ApaI    ApaLI     ApoI     AvaI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI     BclI     BglI    BglII
 Bme1580I    BmgBI     BmrI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI
     BsmI    BsmBI Bsp1286I    BspEI    BspMI    BsrBI    BsrFI   BssHII
   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I     BtgI    BtgZI
     BtsI    CspCI   DraIII     DrdI     EaeI     EarI     EciI  Eco57MI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI    HaeII    Hin4I   HincII
     KasI     KpnI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NspI     PasI    PflMI     PfoI    PpuMI
   PspOMI     PsrI     PstI    PvuII    SacII    SanDI     SbfI     ScaI
    SexAI     SfcI     SfoI    SgrAI     SmaI     SmlI     SphI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XcmI     XmaI     ZraI

 Enzymes that do not cut: 

     AarI     AclI    AflII     AgeI     AloI     AscI     AseI    AsiSI
    AvrII     BaeI    BciVI    BfrBI     BlpI     BmtI     BplI    BsiEI
    BsiWI    BspHI    BsrDI    BsrGI    BssSI   Bsu36I     ClaI     DraI
     EagI  EcoICRI     FseI     FspI    FspAI  HindIII     HpaI     MfeI
     MluI     NheI     NotI     NruI     NsiI     PacI     PciI     PmeI
     PmlI     PpiI    PshAI     PsiI    PspXI     PvuI    RsrII     SacI
     SalI     SapI     SfiI     SgfI    SnaBI     SpeI     SrfI     SspI
     SwaI     XbaI     XhoI     XmnI
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