Restriction map for pF1KB7268
##################################
# NO CUT ENZYMES (  136 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AflII       Alw44I      
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
AscI        Asp718I     AspI        AvaI        AviII       
AvrII       BamHI       BbeI        BbrPI       BbvCI       
BcgI        BciVI       BcnI        BfrI        BglI        
BglII       BlnI        BlpI        Bpu1102I    BsaAI       
BsaHI       BseAI       BseRI       BsgI        BsiEI       
BsiWI       BsoBI       BspDI       BspEI       BsrBI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstZ17I     Bsu36I      
CelII       CfoI        ClaI        CpoI        DraIII      
DrdI        DsaI        EagI        EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO65I     
EcoRV       FauI        FseI        FspI        HaeII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HpaI        KasI        KpnI        
Ksp632I     KspI        MluI        MroI        MspA1I      
MvnI        MwoI        NaeI        NarI        NciI        
NcoI        NgoMIV      NheI        NotI        NruI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PmaCI       PmeI        PmlI        PshAI       Psp1406I    
PstI        PvuI        PvuII       RsrII       SacII       
SalI        SapI        SbfI        ScaI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        Sse8387I    SwaI        Tsp45I      Tth111I     
Van91I      XbaI        XcmI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   55 enzymes) #
###################################

AflIII       1524  ACATGT               A^C[GA][CT]GT
AgeI         1177  ACCGGT               A^CCGGT
AhdI         1467  GACCTTGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI          974  GGATC                GGATC(4/5)
AseI          652  ATTAAT               AT^TAAT
AsnI          652  ATTAAT               AT^TAAT
Asp700I       352  GAATTATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        1467  GACCTTGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI        1227  GAGCTC               G[AT]GC[AT]^C
BalI          125  TGGCCA               TGG^CCA
BanI          162  GGCACC               G^G[CT][GA]CC
BbvI          366  GCAGC                GCAGC(8/12)
BclI          518  TGATCA               T^GATCA
BsaBI         516  GATGATCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI        1177  ACCGGT               [AT]^CCGG[AT]
BsiHKAI      1227  GAGCTC               G[AT]GC[AT]^C
BsmBI         188  GAGACG               CGTCTC(1/5)
BsmFI          96  GTCCC                GGGAC(10/14)
BspHI        1476  TCATGA               T^CATGA
BspLU11I     1524  ACATGT               A^CATGT
BspMI         165  ACCTGC               ACCTGC(4/8)
BsrDI        1393  CATTGC               GCAATG(2/0)
BsrFI        1177  ACCGGT               [GA]^CCGG[CT]
BstXI        1608  CCATATGAATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         974  GGATCT               [GA]^GATC[CT]
Cfr10I       1177  ACCGGT               [GA]^CCGG[CT]
DraI          715  TTTAAA               TTT^AAA
EaeI          125  TGGCCA               [CT]^GGCC[GA]
Eam1105I     1467  GACCTTGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI          518  TGATCA               T^GATCA
Fnu4HI        366  GCAGC                GC^[ACGT]GC
HapII        1178  CCGG                 C^CGG
HpaII        1178  CCGG                 C^CGG
ItaI          366  GCAGC                GC^[ACGT]GC
MaeII         191  ACGT                 A^CGT
MaeIII       1565  GTTAC                ^GT[ACGT]AC
MamI          516  GATGATCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         1402  CAATTG               C^AATTG
MflI          974  GGATCT               [GA]^GATC[CT]
MluNI         125  TGGCCA               TGG^CCA
MscI          125  TGGCCA               TGG^CCA
MspI         1178  CCGG                 C^CGG
MunI         1402  CAATTG               C^AATTG
PinAI        1177  ACCGGT               A^CCGGT
PpuMI          62  AGGACCT              [GA]G^G[AT]CC[CT]
PshBI         652  ATTAAT               AT^TAAT
RcaI         1476  TCATGA               T^CATGA
SacI         1227  GAGCTC               GAGCT^C
SexAI         215  ACCTGGT              A^CC[AT]GGT
SfaNI         559  GCATC                GCATC(5/9)
SfcI          207  CTACAG               C^T[GA][CT]AG
SspI          748  AATATT               AAT^ATT
TseI          366  GCAGC                G^C[AT]GC
XhoII         974  GGATCT               [GA]^GATC[CT]
XmnI          352  GAATTATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AvaII           3  GGACC                G^G[AT]CC
               63  GGACC               
BanII         280  GAGCCC               G[GA]GC[CT]^C
             1227  GAGCTC              
BbsI         1464  GAAGAC               GAAGAC(2/6)
              133  GTCTTC              
BfaI          265  CTAG                 C^TAG
              590  CTAG                
BmyI          280  GAGCCC               G[AGT]GC[ACT]^C
             1227  GAGCTC              
BpmI          308  CTGGAG               CTGGAG(16/14)
             1262  CTGGAG              
BpuAI        1464  GAAGAC               GAAGAC(2/6)
              133  GTCTTC              
BsmI          460  GAATGC               GAATGC(1/-1)
             1505  GAATGC              
Bsp1286I      280  GAGCCC               G[AGT]GC[ACT]^C
             1227  GAGCTC              
Bsp1407I      182  TGTACA               T^GTACA
             1326  TGTACA              
BsrGI         182  TGTACA               T^GTACA
             1326  TGTACA              
BssKI         216  CCTGG                ^CC[ACGT]GG
              284  CCTGG               
BstNI         216  CCTGG                CC^[AT]GG
              284  CCTGG               
DraII          62  AGGACCT              [GA]G^G[ACGT]CC[CT]
             1297  AGGGCCT             
EcoO109I       62  AGGACCT              [GA]G^G[ACGT]CC[CT]
             1297  AGGGCCT             
EcoRII        216  CCTGG                ^CC[AT]GG
              284  CCTGG               
EcoT14I        99  CCTTGG               C^C[AT][AT]GG
              347  CCAAGG              
MaeI          265  CTAG                 C^TAG
              590  CTAG                
MvaI          216  CCTGG                CC^[AT]GG
              284  CCTGG               
ScrFI         216  CCTGG                CC^[ACGT]GG
              284  CCTGG               
SphI          555  GCATGC               GCATG^C
             1027  GCATGC              
SspBI         182  TGTACA               T^GTACA
             1326  TGTACA              
StyI           99  CCTTGG               C^C[AT][AT]GG
              347  CCAAGG              
TaqI          383  TCGA                 T^CGA
              888  TCGA                
TthHB8I       383  TCGA                 T^CGA
              888  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   42 enzymes) #
#########################################

AcsI        AfaI        AluI        ApoI        BsaI        
BsaJI       BsiYI       BslI        BsmAI       BsrI        
BstF5I      Cac8I       Cfr13I      DdeI        DpnII       
EcoRI       EcoT22I     FokI        HaeIII      HinfI       
HphI        MboI        MboII       MnlI        MseI        
MslI        NdeI        NdeII       NlaIII      NlaIV       
NsiI        NspI        PleI        RsaI        Sau3AI      
Sau96I      SmlI        StuI        TfiI        Tru9I       
Tsp509I     TspRI       
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