Restriction map for pF1KB9774
 (Linear) (Six-base) MAPSORT of: KIBB9774.seq  Check: 5355  from: 1  to: 2013



KIBB9774.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:30  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1772    1952    2013 
   Size:       1772     180      61

  Fragments arranged by size: 

                1772     180      61
 
AccI GT'mk_AC

Cuts at:      0     602    2013 
   Size:        602    1411
 
AflIII A'CryG_T

Cuts at:      0    1889    2013 
   Size:       1889     124
 
AhdI GACnn_n'nnGTC

Cuts at:      0     978    1092    2013 
   Size:        978     114     921

  Fragments arranged by size: 

                 978     921     114
 
AlwNI CAG_nnn'CTG

Cuts at:      0      36      51      90     357    1287    1362    1496    1782 
   Size:         36      15      39     267     930      75     134     286

Cuts at:   1782    1848    2013 
   Size:         66     165

  Fragments arranged by size: 

                 930     286     267     165     134      75      66      39
                  36      15
 
ApaI G_GGCC'C

Cuts at:      0      84    1972    2013 
   Size:         84    1888      41

  Fragments arranged by size: 

                1888      84      41
 
ApaLI G'TGCA_C

Cuts at:      0    1811    1951    2013 
   Size:       1811     140      62

  Fragments arranged by size: 

                1811     140      62
 
ApoI r'AATT_y

Cuts at:      0     424    2013 
   Size:        424    1589
 
AvaI C'yCGr_G

Cuts at:      0     558    1314    1326    1366    1561    1674    1965    2013 
   Size:        558     756      12      40     195     113     291      48

  Fragments arranged by size: 

                 756     558     291     195     113      48      40      12
            
 
BamHI G'GATC_C

Cuts at:      0     819    1053    2013 
   Size:        819     234     960

  Fragments arranged by size: 

                 960     819     234
 
BanI G'GyrC_C

Cuts at:      0     231     846    1389    1534    1975    2013 
   Size:        231     615     543     145     441      38

  Fragments arranged by size: 

                 615     543     441     231     145      38
 
BanII G_rGCy'C

Cuts at:      0      84     266     724     761     842    1972    2013 
   Size:         84     182     458      37      81    1130      41

  Fragments arranged by size: 

                1130     458     182      84      81      41      37
 
BbeI G_GCGC'C

Cuts at:      0    1393    1538    1979    2013 
   Size:       1393     145     441      34

  Fragments arranged by size: 

                1393     441     145      34
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     231     632    1747    2013 
   Size:        231     401    1115     266

  Fragments arranged by size: 

                1115     401     266     231
 
BbvCI CC'TCA_GC

Cuts at:      0      29    2013 
   Size:         29    1984
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1560    1594    2013 
   Size:       1560      34     419

  Fragments arranged by size: 

                1560     419      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     316    1676    2013 
   Size:        316    1360     337

  Fragments arranged by size: 

                1360     337     316
 
BglII A'GATC_T

Cuts at:      0     284    2013 
   Size:        284    1729
 
BlpI GC'TnA_GC

Cuts at:      0     755    2013 
   Size:        755    1258
 
Bme1580I G_kGCm'C

Cuts at:      0      84     234    1561    1815    1955    1972    2013 
   Size:         84     150    1327     254     140      17      41

  Fragments arranged by size: 

                1327     254     150     140      84      41      17
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     261    2013 
   Size:        261    1752
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1395    2013 
   Size:       1395     618
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      93     385     523     728     937    2013 
   Size:         93     292     138     205     209    1076

  Fragments arranged by size: 

                1076     292     209     205     138      93
 
Bpu10I CC'TnA_GC

Cuts at:      0      29    2003    2013 
   Size:         29    1974      10

  Fragments arranged by size: 

                1974      29      10
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     152    1837    1918    2013 
   Size:        152    1685      81      95

  Fragments arranged by size: 

                1685     152      95      81
 
BsaAI yAC'GTr

Cuts at:      0    1892    2013 
   Size:       1892     121
 
BsaHI Gr'CG_yC

Cuts at:      0     185    1390    1535    1976    2013 
   Size:        185    1205     145     441      37

  Fragments arranged by size: 

                1205     441     185     145      37
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     433     463     991    1021    1973    2003    2013 
   Size:        433      30     528      30     952      30      10

  Fragments arranged by size: 

                 952     528     433      30      30      30      10
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     115    1012    1126    1513    2013 
   Size:        115     897     114     387     500

  Fragments arranged by size: 

                 897     500     387     115     114
 
BseYI C'CCAG_C

Cuts at:      0      36      77     459    1229    1235    1241    1274    2013 
   Size:         36      41     382     770       6       6      33     739

  Fragments arranged by size: 

                 770     739     382      41      36      33       6       6
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1513    1768    2013 
   Size:       1513     255     245

  Fragments arranged by size: 

                1513     255     245
 
BsiEI CG_ry'CG

Cuts at:      0      22     249     419    2013 
   Size:         22     227     170    1594

  Fragments arranged by size: 

                1594     227     170      22
 
BsiHKAI G_wGCw'C

Cuts at:      0    1729    1763    1815    1955    2013 
   Size:       1729      34      52     140      58

  Fragments arranged by size: 

                1729     140      58      52      34
 
BsmI GAATG_Cn'

Cuts at:      0     155    2013 
   Size:        155    1858
 
Bsp1286I G_dGCh'C

Cuts at:      0      84     234     266     724     761     842    1561    1729 
   Size:         84     150      32     458      37      81     719     168

Cuts at:   1729    1763    1815    1955    1972    2013 
   Size:         34      52     140      17      41

  Fragments arranged by size: 

                 719     458     168     150     140      84      81      52
                  41      37      34      32      17
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1772    1856    1952    2013 
   Size:       1772      84      96      61

  Fragments arranged by size: 

                1772      96      84      61
 
BsrBI CCG'CTC

Cuts at:      0    1629    1713    1797    2013 
   Size:       1629      84      84     216

  Fragments arranged by size: 

                1629     216      84      84
 
BsrDI GCAATG_nn'

Cuts at:      0     605    2013 
   Size:        605    1408
 
BsrFI r'CCGG_y

Cuts at:      0     120    2013 
   Size:        120    1893
 
BssSI C'ACGA_G

Cuts at:      0    1554    2013 
   Size:       1554     459
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1496    1848    2013 
   Size:       1496     352     165

  Fragments arranged by size: 

                1496     352     165
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1127    1302    2013 
   Size:       1127     175     711

  Fragments arranged by size: 

                1127     711     175
 
BstYI r'GATC_y

Cuts at:      0     284     819    1053    2013 
   Size:        284     535     234     960

  Fragments arranged by size: 

                 960     535     284     234
 
BtgI C'CryG_G

Cuts at:      0     419    1049    1126    1704    1981    2013 
   Size:        419     630      77     578     277      32

  Fragments arranged by size: 

                 630     578     419     277      77      32
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1281    1445    2013 
   Size:       1281     164     568

  Fragments arranged by size: 

                1281     568     164
 
DraIII CAC_nnn'GTG

Cuts at:      0     893    2013 
   Size:        893    1120
 
EaeI y'GGCC_r

Cuts at:      0      19     140     193     398     416    1304    2013 
   Size:         19     121      53     205      18     888     709

  Fragments arranged by size: 

                 888     709     205     121      53      19      18
 
EagI C'GGCC_G

Cuts at:      0      19     416    2013 
   Size:         19     397    1597

  Fragments arranged by size: 

                1597     397      19
 
EarI CTCTTCn'nnn_

Cuts at:      0     794    1651    2013 
   Size:        794     857     362

  Fragments arranged by size: 

                 857     794     362
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1530    1675    2013 
   Size:       1530     145     338

  Fragments arranged by size: 

                1530     338     145
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      93     385     523     728     937    2013 
   Size:         93     292     138     205     209    1076

  Fragments arranged by size: 

                1076     292     209     205     138      93
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     472     679    2013 
   Size:        472     207    1334

  Fragments arranged by size: 

                1334     472     207
 
EcoO109I rG'GnC_Cy

Cuts at:      0      48     464     543     576     912     972    1969    2013 
   Size:         48     416      79      33     336      60     997      44

  Fragments arranged by size: 

                 997     416     336      79      60      48      44      33
            
 
FspI TGC'GCA

Cuts at:      0     526     854    1588    2013 
   Size:        526     328     734     425

  Fragments arranged by size: 

                 734     526     425     328
 
HaeII r_GCGC'y

Cuts at:      0     127     792    1393    1538    1884    1979    2013 
   Size:        127     665     601     145     346      95      34

  Fragments arranged by size: 

                 665     601     346     145     127      95      34
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     850     882     964     996    1078    1110    2013 
   Size:        850      32      82      32      82      32     903

  Fragments arranged by size: 

                 903     850      82      82      32      32      32
 
HincII GTy'rAC

Cuts at:      0      14     603    2013 
   Size:         14     589    1410

  Fragments arranged by size: 

                1410     589      14
 
KasI G'GCGC_C

Cuts at:      0    1389    1534    1975    2013 
   Size:       1389     145     441      38

  Fragments arranged by size: 

                1389     441     145      38
 
MslI CAynn'nnrTG

Cuts at:      0     610    1125    1300    2013 
   Size:        610     515     175     713

  Fragments arranged by size: 

                 713     610     515     175
 
MspA1I CmG'CkG

Cuts at:      0      36      65     354     909    1260    1493    1706    1779 
   Size:         36      29     289     555     351     233     213      73

Cuts at:   1779    1958    2013 
   Size:        179      55

  Fragments arranged by size: 

                 555     351     289     233     213     179      73      55
                  36      29
 
NaeI GCC'GGC

Cuts at:      0     122    2013 
   Size:        122    1891
 
NarI GG'CG_CC

Cuts at:      0    1390    1535    1976    2013 
   Size:       1390     145     441      37

  Fragments arranged by size: 

                1390     441     145      37
 
NcoI C'CATG_G

Cuts at:      0    1126    2013 
   Size:       1126     887
 
NgoMIV G'CCGG_C

Cuts at:      0     120    2013 
   Size:        120    1893
 
PasI CC'CwG_GG

Cuts at:      0     761    2013 
   Size:        761    1252
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      51    2013 
   Size:         51    1962
 
PfoI T'CCnGG_A

Cuts at:      0     982    2013 
   Size:        982    1031
 
PmlI CAC'GTG

Cuts at:      0    1892    2013 
   Size:       1892     121
 
PpuMI rG'GwC_Cy

Cuts at:      0     464     543     972    2013 
   Size:        464      79     429    1041

  Fragments arranged by size: 

                1041     464     429      79
 
PshAI GACnn'nnGTC

Cuts at:      0     598    2013 
   Size:        598    1415
 
PsiI TTA'TAA

Cuts at:      0     668    2013 
   Size:        668    1345
 
PspOMI G'GGCC_C

Cuts at:      0      80    1968    2013 
   Size:         80    1888      45

  Fragments arranged by size: 

                1888      80      45
 
PstI C_TGCA'G

Cuts at:      0     476     494    1265    1769    2013 
   Size:        476      18     771     504     244

  Fragments arranged by size: 

                 771     504     476     244      18
 
PvuII CAG'CTG

Cuts at:      0      36     354    1260    1493    2013 
   Size:         36     318     906     233     520

  Fragments arranged by size: 

                 906     520     318     233      36
 
SacII CC_GC'GG

Cuts at:      0    1707    2013 
   Size:       1707     306
 
SalI G'TCGA_C

Cuts at:      0     601    2013 
   Size:        601    1412
 
SanDI GG'GwC_CC

Cuts at:      0     464    2013 
   Size:        464    1549
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1651    2013 
   Size:       1651     362
 
SfcI C'TryA_G

Cuts at:      0     472     490    1261    1746    1765    2013 
   Size:        472      18     771     485      19     248

  Fragments arranged by size: 

                 771     485     472     248      19      18
 
SfoI GGC'GCC

Cuts at:      0    1391    1536    1977    2013 
   Size:       1391     145     441      36

  Fragments arranged by size: 

                1391     441     145      36
 
SmaI CCC'GGG

Cuts at:      0     560    1316    1368    1967    2013 
   Size:        560     756      52     599      46

  Fragments arranged by size: 

                 756     599     560      52      46
 
SmlI C'TyrA_G

Cuts at:      0     167    1852    1933    2013 
   Size:        167    1685      81      80

  Fragments arranged by size: 

                1685     167      81      80
 
SrfI GCCC'GGGC

Cuts at:      0    1967    2013 
   Size:       1967      46
 
StyI C'CwwG_G

Cuts at:      0     581    1126    1622    2013 
   Size:        581     545     496     391

  Fragments arranged by size: 

                 581     545     496     391
 
TatI w'GTAC_w

Cuts at:      0     614    2013 
   Size:        614    1399
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     224     256     433     465     877     909     991    1023 
   Size:        224      32     177      32     412      32      82      32

Cuts at:   1023    1973    2005    2013 
   Size:        950      32       8

  Fragments arranged by size: 

                 950     412     224     177      82      32      32      32
                  32      32       8
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     318    2013 
   Size:        318    1695
 
XmaI C'CCGG_G

Cuts at:      0     558    1314    1366    1965    2013 
   Size:        558     756      52     599      48

  Fragments arranged by size: 

                 756     599     558      52      48
 
XmnI GAAnn'nnTTC

Cuts at:      0    1660    2013 
   Size:       1660     353
 

 Enzymes that do cut:

     AarI     AccI   AflIII     AhdI    AlwNI     ApaI    ApaLI     ApoI
     AvaI    BamHI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI
     BglI    BglII     BlpI Bme1580I     BmrI     BplI     BpmI   Bpu10I
    BpuEI    BsaAI    BsaHI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI     BsmI Bsp1286I    BspMI    BsrBI    BsrDI    BsrFI    BssSI
   BstAPI    BstXI    BstYI     BtgI    BtgZI   DraIII     EaeI     EagI
     EarI     EciI  Eco57MI    EcoNI EcoO109I     FspI    HaeII    Hin4I
   HincII     KasI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV
     PasI    PflMI     PfoI     PmlI    PpuMI    PshAI     PsiI   PspOMI
     PstI    PvuII    SacII     SalI    SanDI     SapI     SfcI     SfoI
     SmaI     SmlI     SrfI     StyI     TatI     TstI     XcmI     XmaI
     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AcuI     AfeI    AflII     AgeI     AleI
     AlfI     AloI     AscI     AseI    AsiSI    AvrII     BaeI    BciVI
     BclI    BfrBI    BmgBI     BmtI     BsaI    BsaBI    BsaWI    BsiWI
    BsmBI    BspEI    BspHI    BsrGI   BssHII    BstBI   BstEII  BstZ17I
   Bsu36I     BtsI     ClaI    CspCI     DraI     DrdI  EcoICRI    EcoRI
    EcoRV     FalI     FseI    FspAI  HindIII     HpaI     KpnI     MfeI
     MluI     MmeI     MscI     NdeI     NheI     NotI     NruI     NsiI
     NspI     PacI     PciI     PmeI     PpiI    PspXI     PsrI     PvuI
    RsrII     SacI     SbfI     ScaI    SexAI     SfiI     SgfI    SgrAI
    SnaBI     SpeI     SphI     SspI     StuI     SwaI    TaqII  Tth111I
     XbaI     XhoI     ZraI
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