Restriction map for pF1KE1028
##################################
# NO CUT ENZYMES (   96 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AcyI        
AflII       AhdI        Alw44I      AocI        ApaI        
ApaLI       AscI        Asp718I     AspEI       AvrII       
BamHI       BbeI        BbrPI       BbvCI       BfrI        
BglI        BlnI        BsaAI       BsaBI       BsaHI       
BseAI       BseRI       BsgI        BsiEI       BsiWI       
BspEI       BsrBI       BsrDI       BssHII      Bst1107I    
BstUI       BstZ17I     Bsu36I      CpoI        DraII       
DraIII      DrdI        DsaI        EagI        Eam1105I    
EclXI       Eco52I      Eco81I      EcoNI       EcoO109I    
EcoRI       EcoT22I     FauI        FseI        Hin1I       
KasI        KpnI        KspI        MamI        MluI        
MroI        MvnI        NaeI        NarI        NcoI        
NgoMIV      NheI        NotI        NruI        NsiI        
PacI        PmaCI       PmeI        PmlI        PpuMI       
PshAI       PstI        PvuI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SfcI        SfiI        SfoI        SgrAI       SmaI        
SnaBI       Sse8387I    SwaI        XcmI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AccI         2313  GTCTAC               GT^[AC][GT]AC
AciI          180  GCGG                 CCGC(-3/-1)
AclI          971  AACGTT               AA^CGTT
AflIII       1872  ACATGT               A^C[GA][CT]GT
AgeI          260  ACCGGT               A^CCGGT
AspHI        1985  GTGCTC               G[AT]GC[AT]^C
AspI         1966  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII        2099  TGCGCA               TGC^GCA
BalI         3614  TGGCCA               TGG^CCA
BanI          307  GGTGCC               G^G[CT][GA]CC
BanII         662  GAGCCC               G[GA]GC[CT]^C
BbsI         1261  GAAGAC               GAAGAC(2/6)
BcgI          699  CGAGACTCGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        1788  GGATAC               GTATCC(6/5)
BcnI         3405  CCCGG                CC^[GC]GG
BglII        1464  AGATCT               A^GATCT
BpuAI        1261  GAAGAC               GAAGAC(2/6)
BsaI         2164  GAGACC               GGTCTC(1/5)
BsaWI         260  ACCGGT               [AT]^CCGG[AT]
BsiHKAI      1985  GTGCTC               G[AT]GC[AT]^C
BsmBI         657  GAGACG               CGTCTC(1/5)
BsmFI        3259  GTCCC                GGGAC(10/14)
BsmI         1428  GAATGC               GAATGC(1/-1)
BspHI        2748  TCATGA               T^CATGA
BspLU11I     1872  ACATGT               A^CATGT
BsrFI         260  ACCGGT               [GA]^CCGG[CT]
BstXI        3273  CCAGTTTACTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I        260  ACCGGT               [GA]^CCGG[CT]
EaeI         3614  TGGCCA               [CT]^GGCC[GA]
EcoRV         805  GATATC               GAT^ATC
FspI         2099  TGCGCA               TGC^GCA
HgaI         3006  GCGTC                GACGC(5/10)
MfeI           53  CAATTG               C^AATTG
MluNI        3614  TGGCCA               TGG^CCA
MscI         3614  TGGCCA               TGG^CCA
MunI           53  CAATTG               C^AATTG
NciI         3405  CCCGG                CC^[GC]GG
NdeI         2731  CATATG               CA^TATG
PflFI        1966  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        3438  CCAGGCTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI         260  ACCGGT               A^CCGGT
Psp1406I      971  AACGTT               AA^CGTT
RcaI         2748  TCATGA               T^CATGA
SexAI        3144  ACCTGGT              A^CC[AT]GGT
SpeI          742  ACTAGT               A^CTAGT
StuI         3526  AGGCCT               AGG^CCT
Tth111I      1966  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       3438  CCAGGCTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   40 enzymes) #
###################################

Aor51HI       379  AGCGCT               AGC^GCT
             2705  AGCGCT              
AseI         1216  ATTAAT               AT^TAAT
             2119  ATTAAT              
AsnI         1216  ATTAAT               AT^TAAT
             2119  ATTAAT              
Asp700I      2250  GAAAACCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3079  GAAGCTATTC          
AvaI         1337  CTCGAG               C^[CT]CG[GA]G
             2477  CTCGAG              
BmyI          662  GAGCCC               G[AGT]GC[ACT]^C
             1985  GTGCTC              
BsiYI        2168  CCCAGCAAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
             3438  CCAGGCTCTGG         
BslI         2168  CCCAGCAAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
             3438  CCAGGCTCTGG         
BsoBI        1337  CTCGAG               C^[CT]CG[GA]G
             2477  CTCGAG              
Bsp1286I      662  GAGCCC               G[AGT]GC[ACT]^C
             1985  GTGCTC              
Bsp1407I        2  TGTACA               T^GTACA
             3495  TGTACA              
BspDI         271  ATCGAT               AT^CGAT
             3036  ATCGAT              
BspMI        2096  ACCTGC               ACCTGC(4/8)
               12  GCAGGT              
BsrGI           2  TGTACA               T^GTACA
             3495  TGTACA              
BssSI         212  CTCGTG               CACGAG(-5/-1)
              704  CTCGTG              
BstEII        263  GGTTACC              G^GT[ACGT]ACC
             1963  GGTGACC             
BstPI         263  GGTTACC              G^GT[ACGT]ACC
             1963  GGTGACC             
ClaI          271  ATCGAT               AT^CGAT
             3036  ATCGAT              
DraI         1515  TTTAAA               TTT^AAA
             2182  TTTAAA              
Eco47III      379  AGCGCT               AGC^GCT
             2705  AGCGCT              
EcoO65I       263  GGTTACC              G^GT[ACGT]ACC
             1963  GGTGACC             
EcoT14I       926  CCAAGG               C^C[AT][AT]GG
             3362  CCTTGG              
HaeII         379  AGCGCT               [GA]GCGC^[CT]
             2705  AGCGCT              
HapII         261  CCGG                 C^CGG
             3406  CCGG                
HindIII      1862  AAGCTT               A^AGCTT
             2295  AAGCTT              
HpaI         2944  GTTAAC               GTT^AAC
             3090  GTTAAC              
HpaII         261  CCGG                 C^CGG
             3406  CCGG                
MaeII         972  ACGT                 A^CGT
             2271  ACGT                
MspI          261  CCGG                 C^CGG
             3406  CCGG                
NlaIV         307  GGTGCC               GG[ACGT]^[ACGT]CC
             3408  GGCTCC              
PaeR7I       1337  CTCGAG               C^TCGAG
             2477  CTCGAG              
PshBI        1216  ATTAAT               AT^TAAT
             2119  ATTAAT              
PvuII        1921  CAGCTG               CAG^CTG
             3545  CAGCTG              
SphI         2301  GCATGC               GCATG^C
             2789  GCATGC              
SspBI           2  TGTACA               T^GTACA
             3495  TGTACA              
SspI         1361  AATATT               AAT^ATT
             2107  AATATT              
StyI          926  CCAAGG               C^C[AT][AT]GG
             3362  CCTTGG              
XbaI          606  TCTAGA               T^CTAGA
             2191  TCTAGA              
XhoI         1337  CTCGAG               C^TCGAG
             2477  CTCGAG              
XmnI         2250  GAAAACCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3079  GAAGCTATTC          


#########################################
# 3 OR MORE CUT ENZYMES (   74 enzymes) #
#########################################

AcsI        AfaI        AluI        AlwI        AlwNI       
ApoI        AvaII       BbvI        BclI        BfaI        
BlpI        BpmI        Bpu1102I    BsaJI       BsmAI       
BsrI        BssKI       BstAPI      BstBI       BstF5I      
BstNI       BstYI       Cac8I       CelII       CfoI        
Cfr13I      DdeI        DpnII       EarI        EcoRII      
FbaI        Fnu4HI      FokI        HaeIII      HhaI        
HinP1I      HincII      HindII      HinfI       HphI        
ItaI        Ksp632I     MaeI        MaeIII      MboI        
MboII       MflI        MnlI        MseI        MslI        
MspA1I      MvaI        MwoI        NdeII       NlaIII      
NspI        NspV        PleI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfuI        SmlI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XhoII       
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