Restriction map for pF1KB9284
##################################
# NO CUT ENZYMES (  165 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AclI        
AcsI        AcyI        AflII       AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     AspEI       
AspHI       AspI        AvaI        AviII       AvrII       
BamHI       BbeI        BbrPI       BbsI        BbvCI       
BcgI        BclI        BfrI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BpuAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BsiEI       
BsiHKAI     BsiWI       BsmAI       BsmBI       BsmI        
BsoBI       Bsp1407I    BspDI       BspEI       BspHI       
BsrBI       BsrDI       BsrFI       BsrGI       BsrI        
BssHII      BssSI       Bst1107I    BstBI       BstUI       
BstZ17I     Bsu36I      CelII       CfoI        Cfr10I      
ClaI        CpoI        DraI        DraIII      DrdI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        HaeII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HindIII     HpaI        KasI        Ksp632I     KspI        
MamI        MfeI        MluI        MroI        MseI        
MunI        MvnI        NaeI        NarI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        RcaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfaNI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
Sse8387I    SspBI       SspI        StuI        SwaI        
TaqI        Tru9I       Tth111I     TthHB8I     Van91I      
XbaI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

Acc65I        475  GGTACC               G^GTACC
AflIII        119  ACATGT               A^C[GA][CT]GT
AlwI           22  GGATC                GGATC(4/5)
ApaI          542  GGGCCC               GGGCC^C
Asp718I       475  GGTACC               G^GTACC
AvaII         452  GGACC                G^G[AT]CC
BalI          461  TGGCCA               TGG^CCA
BanI          475  GGTACC               G^G[CT][GA]CC
BciVI         123  GTATCC               GTATCC(6/5)
BfaI          416  CTAG                 C^TAG
BpmI          128  CTGGAG               CTGGAG(16/14)
BsgI          364  GTGCAG               GTGCAG(16/14)
BsmFI         451  GGGAC                GGGAC(10/14)
BspLU11I      119  ACATGT               A^CATGT
BspMI         472  GCAGGT               ACCTGC(4/8)
BstAPI         97  GCAGCTCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        429  GGTCACC              G^GT[ACGT]ACC
BstPI         429  GGTCACC              G^GT[ACGT]ACC
BstXI         434  CCATGGGCATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI          22  GGATCT               [GA]^GATC[CT]
DdeI          578  CTCAG                C^T[ACGT]AG
DpnII          23  GATC                 ^GATC
DraII         542  GGGCCCC              [GA]G^G[ACGT]CC[CT]
EaeI          461  TGGCCA               [CT]^GGCC[GA]
EcoNI         412  CCTCCTAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      542  GGGCCCC              [GA]G^G[ACGT]CC[CT]
EcoO65I       429  GGTCACC              G^GT[ACGT]ACC
FauI          200  GCGGG                CCCGC(4/6)
HphI          431  TCACC                GGTGA(8/7)
KpnI          475  GGTACC               GGTAC^C
MaeI          416  CTAG                 C^TAG
MboI           23  GATC                 ^GATC
MboII          25  TCTTC                GAAGA(8/7)
MflI           22  GGATCT               [GA]^GATC[CT]
MluNI         461  TGGCCA               TGG^CCA
MscI          461  TGGCCA               TGG^CCA
NdeII          23  GATC                 ^GATC
PvuII          68  CAGCTG               CAG^CTG
Sau3AI         23  GATC                 ^GATC
SphI          377  GCATGC               GCATG^C
Tsp509I        57  AATT                 ^AATT
XcmI          434  CCATGGGCATGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII          22  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AciI          319  CCGC                 CCGC(-3/-1)
              200  GCGG                
AlwNI          98  CAGCTCCTG            CAG[ACGT][ACGT][ACGT]^CTG
              316  CAGCCGCTG           
BanII         282  GAGCCC               G[GA]GC[CT]^C
              542  GGGCCC              
BcnI          519  CCCGG                CC^[GC]GG
              540  CCGGG               
BsaAI         303  CACGTA               [CT]AC^GT[GA]
              534  CACGTA              
BstF5I        345  CATCC                GGATG(2/0)
              405  CATCC               
Cac8I         377  GCATGC               GC[ACGT]^[ACGT]GC
              381  GCCTGC              
FokI          345  CATCC                GGATG(9/13)
              405  CATCC               
HaeIII        462  GGCC                 GG^CC
              543  GGCC                
HapII         520  CCGG                 C^CGG
              540  CCGG                
HinfI           3  GAATC                G^A[ACGT]TC
              135  GAATC               
HpaII         520  CCGG                 C^CGG
              540  CCGG                
MspI          520  CCGG                 C^CGG
              540  CCGG                
NciI          519  CCCGG                CC^[GC]GG
              540  CCGGG               
NspI          119  ACATGT               [GA]CATG^[CT]
              377  GCATGC              
TfiI            3  GAATC                G^A[AT]TC
              135  GAATC               
Tsp45I        430  GTCAC                ^GT[GC]AC
              523  GTCAC               
TspRI         525  CACTG                CA[GC]TG[ACGT][ACGT]^
              580  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   32 enzymes) #
#########################################

AfaI        AluI        BbvI        BmyI        BsaJI       
BseRI       BsiYI       BslI        Bsp1286I    BssKI       
BstNI       Cfr13I      DsaI        EcoRII      EcoT14I     
Fnu4HI      ItaI        MaeII       MaeIII      MnlI        
MslI        MspA1I      MvaI        MwoI        NcoI        
NlaIII      NlaIV       RsaI        Sau96I      ScrFI       
StyI        TseI        
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