Restriction map for
pF1KB9044
(Circular) (Six-base) MAPSORT of: pF1KB9044.seq Check: 6647 from: 1 to: 4703
pF1KB9044 4703 bp
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AccI GT'mk_AC
Cuts at: 1741 1741
Size: 4703
Acc65I G'GTAC_C
Cuts at: 1719 1719
Size: 4703
AclI AA'CG_TT
Cuts at: 4543 4543
Size: 4703
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 401 2528 2960 3707 3975 401
Size: 2127 432 747 268 1129
Fragments arranged by size:
2127 1129 747 432 268
AflIII A'CryG_T
Cuts at: 2055 3159 4136 2055
Size: 1104 977 2622
Fragments arranged by size:
2622 1104 977
AgeI A'CCGG_T
Cuts at: 1607 3091 1607
Size: 1484 3219
AlwNI CAG_nnn'CTG
Cuts at: 2008 3575 2008
Size: 1567 3136
ApaLI G'TGCA_C
Cuts at: 3473 3473
Size: 4703
ApoI r'AATT_y
Cuts at: 169 213 462 601 609 713 729 797 881
Size: 44 249 139 8 104 16 68 84
Cuts at: 881 1049 1133 1217 1301 1385 1553 1637 1707
Size: 168 84 84 84 84 168 84 70
Cuts at: 1707 4056 4312 169
Size: 2349 256 560
Fragments arranged by size:
2349 560 256 249 168 168 139 104
84 84 84 84 84 84 70 68
44 16 8
AseI AT'TA_AT
Cuts at: 20 20
Size: 4703
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4703
AvaI C'yCGr_G
Cuts at: 1723 1723
Size: 4703
BamHI G'GATC_C
Cuts at: 1728 1728
Size: 4703
BanI G'GyrC_C
Cuts at: 1719 2133 2381 2416 1719
Size: 414 248 35 4006
Fragments arranged by size:
4006 414 248 35
BanII G_rGCy'C
Cuts at: 320 1717 2747 320
Size: 1397 1030 2276
Fragments arranged by size:
2276 1397 1030
BbeI G_GCGC'C
Cuts at: 2137 2385 2137
Size: 248 4455
BbsI GAAGACnn'nnnn_
Cuts at: 249 389 249
Size: 140 4563
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4669
BciVI GTATCCnnnnn_n'
Cuts at: 2601 3362 4703 2601
Size: 761 1341 2601
Fragments arranged by size:
2601 1341 761
BclI T'GATC_A
Cuts at: 2223 2223
Size: 4703
BglII A'GATC_T
Cuts at: 2218 4255 2218
Size: 2037 2666
BlpI GC'TnA_GC
Cuts at: 1815 1815
Size: 4703
Bme1580I G_kGCm'C
Cuts at: 2328 2421 3477 2328
Size: 93 1056 3554
Fragments arranged by size:
3554 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 352 1915 1996 2331 4475 352
Size: 1563 81 335 2144 580
Fragments arranged by size:
2144 1563 580 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3070 3070
Size: 4703
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 199 995 1079 1163 1247 1331 1347 1499 199
Size: 796 84 84 84 84 16 152 3403
Fragments arranged by size:
3403 796 152 84 84 84 84 16
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1879 2758 3250 3548 1879
Size: 879 492 298 3034
Fragments arranged by size:
3034 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4407 4407
Size: 4703
BsaAI yAC'GTr
Cuts at: 585 2056 585
Size: 1471 3232
BsaBI GATnn'nnATC
Cuts at: 6 2217 4254 6
Size: 2211 2037 455
Fragments arranged by size:
2211 2037 455
BsaHI Gr'CG_yC
Cuts at: 2134 2382 3084 4580 2134
Size: 248 702 1496 2257
Fragments arranged by size:
2257 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1607 1892 2413 3091 3365 3512 3923 1607
Size: 285 521 678 274 147 411 2387
Fragments arranged by size:
2387 678 521 411 285 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 196 196
Size: 4703
BseYI C'CCAG_C
Cuts at: 2128 2886 3463 3959 2128
Size: 758 577 496 2872
Fragments arranged by size:
2872 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 188 188
Size: 4703
BsiEI CG_ry'CG
Cuts at: 104 2245 2291 3499 104
Size: 2141 46 1208 1308
Fragments arranged by size:
2141 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1363 1717 2495 2685 3477 1363
Size: 354 778 190 792 2589
Fragments arranged by size:
2589 792 778 354 190
BsmI GAATG_Cn'
Cuts at: 1258 1258
Size: 4703
Bsp1286I G_dGCh'C
Cuts at: 320 1363 1717 2328 2421 2495 2685 2747 3477
Size: 1043 354 611 93 74 190 62 730
Cuts at: 3477 320
Size: 1546
Fragments arranged by size:
1546 1043 730 611 354 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3923 3923
Size: 4703
BspHI T'CATG_A
Cuts at: 3879 3879
Size: 4703
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1753 1967 2269 2650 1753
Size: 214 302 381 3806
Fragments arranged by size:
3806 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2995 3049 4533 4698 50
Size: 2945 54 1484 165 55
Fragments arranged by size:
2945 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 196 1518 2615 196
Size: 1322 1097 2284
Fragments arranged by size:
2284 1322 1097
BsrFI r'CCGG_y
Cuts at: 1607 2701 2882 3091 1607
Size: 1094 181 209 3219
Fragments arranged by size:
3219 1094 209 181
BssSI C'ACGA_G
Cuts at: 2974 3332 2974
Size: 358 4345
BstAPI GCAn_nnn'nTGC
Cuts at: 1537 2080 1537
Size: 543 4160
BstBI TT'CG_AA
Cuts at: 3064 3064
Size: 4703
BstYI r'GATC_y
Cuts at: 1 1728 2195 2218 2553 2799 3811 3897 3909
Size: 1727 467 23 335 246 1012 86 12
Cuts at: 3909 4255 1
Size: 346 449
Fragments arranged by size:
1727 1012 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2653 2834 2997 2653
Size: 181 163 4359
Fragments arranged by size:
4359 181 163
BtsI GCAGTG_nn'
Cuts at: 200 1946 200
Size: 1746 2957
DraI TTT'AAA
Cuts at: 1702 1702
Size: 4703
DrdI GACnn_nn'nnGTC
Cuts at: 2409 3267 2409
Size: 858 3845
EaeI y'GGCC_r
Cuts at: 161 391 2288 2462 2853 2880 4225 161
Size: 230 1897 174 391 27 1345 639
Fragments arranged by size:
1897 1345 639 391 230 174 27
EagI C'GGCC_G
Cuts at: 2288 2288
Size: 4703
EarI CTCTTCn'nnn_
Cuts at: 325 887 2726 2936 325
Size: 562 1839 210 2092
Fragments arranged by size:
2092 1839 562 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2911 3221 3367 4513 2911
Size: 310 146 1146 3101
Fragments arranged by size:
3101 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1715 1715
Size: 4703
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 199 401 995 1079 1163 1247 1331 1347 1499
Size: 202 594 84 84 84 84 16 152
Cuts at: 1499 2528 2960 3707 3975 199
Size: 1029 432 747 268 927
Fragments arranged by size:
1029 927 747 594 432 268 202 152
84 84 84 84 16
EcoNI CCTnn'n_nnAGG
Cuts at: 324 324
Size: 4703
EcoO109I rG'GnC_Cy
Cuts at: 1842 1842
Size: 4703
EcoRI G'AATT_C
Cuts at: 169 713 729 797 1049 1301 1385 1553 1707
Size: 544 16 68 252 252 84 168 154
Cuts at: 1707 169
Size: 3165
Fragments arranged by size:
3165 544 252 252 168 154 84 68
16
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2722 2722
Size: 4703
FspI TGC'GCA
Cuts at: 2484 2484
Size: 4703
HaeII r_GCGC'y
Cuts at: 2137 2385 3407 4386 2137
Size: 248 1022 979 2454
Fragments arranged by size:
2454 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2545 2575 2577 2607 2545
Size: 30 2 30 4641
Fragments arranged by size:
4641 30 30 2
HincII GTy'rAC
Cuts at: 1742 1742
Size: 4703
HindIII A'AGCT_T
Cuts at: 1178 1262 1346 1598 1178
Size: 84 84 252 4283
Fragments arranged by size:
4283 252 84 84
KasI G'GCGC_C
Cuts at: 2133 2381 2133
Size: 248 4455
KpnI G_GTAC'C
Cuts at: 1723 1723
Size: 4703
MluI A'CGCG_T
Cuts at: 4136 4136
Size: 4703
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3374 3558 3374
Size: 184 4519
MscI TGG'CCA
Cuts at: 163 393 2464 163
Size: 230 2071 2402
Fragments arranged by size:
2402 2071 230
MslI CAynn'nnrTG
Cuts at: 1646 2692 2819 1646
Size: 1046 127 3530
Fragments arranged by size:
3530 1046 127
MspA1I CmG'CkG
Cuts at: 1814 2128 2488 3501 3746 1814
Size: 314 360 1013 245 2771
Fragments arranged by size:
2771 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2884 2884
Size: 4703
NarI GG'CG_CC
Cuts at: 2134 2382 2134
Size: 248 4455
NdeI CA'TA_TG
Cuts at: 803 1295 803
Size: 492 4211
NgoMIV G'CCGG_C
Cuts at: 2882 2882
Size: 4703
NspI r_CATG'y
Cuts at: 687 1756 2256 3163 687
Size: 1069 500 907 2227
Fragments arranged by size:
2227 1069 907 500
PciI A'CATG_T
Cuts at: 3159 3159
Size: 4703
PmeI GTTT'AAAC
Cuts at: 1702 1702
Size: 4703
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3842 3874 3842
Size: 32 4671
PsiI TTA'TAA
Cuts at: 524 524
Size: 4703
PstI C_TGCA'G
Cuts at: 1750 2435 1750
Size: 685 4018
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4703
PvuII CAG'CTG
Cuts at: 2128 2488 2128
Size: 360 4343
RsrII CG'GwC_CG
Cuts at: 2898 2898
Size: 4703
SacI G_AGCT'C
Cuts at: 1717 1717
Size: 4703
SalI G'TCGA_C
Cuts at: 1740 1740
Size: 4703
SapI GCTCTTCn'nnn_
Cuts at: 2726 2936 2726
Size: 210 4493
SbfI CC_TGCA'GG
Cuts at: 1750 1750
Size: 4703
ScaI AGT'ACT
Cuts at: 4220 4220
Size: 4703
SexAI A'CCwGG_T
Cuts at: 251 251
Size: 4703
SfcI C'TryA_G
Cuts at: 33 243 1746 2431 3424 3615 33
Size: 210 1503 685 993 191 1121
Fragments arranged by size:
1503 1121 993 685 210 191
SfoI GGC'GCC
Cuts at: 2135 2383 2135
Size: 248 4455
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4703
SmaI CCC'GGG
Cuts at: 1725 1725
Size: 4703
SmlI C'TyrA_G
Cuts at: 1858 2773 3265 3527 1858
Size: 915 492 262 3034
Fragments arranged by size:
3034 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4703
SphI G_CATG'C
Cuts at: 687 1756 2256 687
Size: 1069 500 3134
Fragments arranged by size:
3134 1069 500
SspI AAT'ATT
Cuts at: 566 566
Size: 4703
StyI C'CwwG_G
Cuts at: 1837 2191 1837
Size: 354 4349
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1346 2286 2419 2878 3087 1346
Size: 940 133 459 209 2962
Fragments arranged by size:
2962 940 459 209 133
TatI w'GTAC_w
Cuts at: 4218 4218
Size: 4703
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4220 4252 4220
Size: 32 4671
Tth111I GACn'n_nGTC
Cuts at: 2500 2500
Size: 4703
XbaI T'CTAG_A
Cuts at: 1734 1734
Size: 4703
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2198 2198
Size: 4703
XmaI C'CCGG_G
Cuts at: 1723 1723
Size: 4703
XmnI GAAnn'nnTTC
Cuts at: 649 901 1069 649
Size: 252 168 4283
Fragments arranged by size:
4283 252 168
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NdeI NgoMIV NspI
PciI PmeI PpiI PsiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BfrBI BglI BmgBI
BmtI Bpu10I BsaXI BsiWI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoRV
FseI FspAI HpaI MfeI NcoI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI
PspOMI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI
SrfI StuI SwaI XhoI ZraI