Restriction map for pF1KB9558
##################################
# NO CUT ENZYMES (  102 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AclI        
AflII       AflIII      Alw44I      Aor51HI     ApaLI       
AscI        AseI        AsnI        Asp718I     AspI        
AvrII       BalI        BbrPI       BciVI       BclI        
BfrI        BlnI        BlpI        Bpu1102I    BsaAI       
BsaBI       BsiWI       BsmBI       BsmI        BspDI       
BspLU11I    BspMI       BssHII      Bst1107I    BstBI       
BstUI       BstXI       BstZ17I     CelII       ClaI        
CpoI        DraI        DraIII      DrdI        EaeI        
EagI        EclXI       Eco47III    Eco52I      EcoRV       
EcoT22I     FbaI        FseI        HincII      HindII      
HpaI        KpnI        KspI        MamI        MfeI        
MluI        MluNI       MscI        MunI        MvnI        
NdeI        NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfuI        SgrAI       SnaBI       SpeI        Sse8387I    
SspI        SwaI        TaqI        Tth111I     TthHB8I     
XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AccIII        315  TCCGGA               T^CCGGA
AgeI         1016  ACCGGT               A^CCGGT
AhdI          380  GACAACAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI          946  CCTCAGG              CC^T[ACGT]AGG
AspEI         380  GACAACAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI        1277  GAGCAC               G[AT]GC[AT]^C
AviII         221  TGCGCA               TGC^GCA
BbeI         1035  GGCGCC               GGCGC^C
BbvCI         174  CCTCAGC              CCTCAGC(-5/-2)
BcgI         1106  CGAAGGAACTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaI         1968  GAGACC               GGTCTC(1/5)
BseAI         315  TCCGGA               T^CCGGA
BsiHKAI      1277  GAGCAC               G[AT]GC[AT]^C
Bsp1407I      671  TGTACA               T^GTACA
BspEI         315  TCCGGA               T^CCGGA
BsrBI         321  GAGCGG               CCGCTC(-3/-3)
BsrDI         667  GCAATG               GCAATG(2/0)
BsrGI         671  TGTACA               T^GTACA
BssSI         449  CACGAG               CACGAG(-5/-1)
BstEII        336  GGTGACC              G^GT[ACGT]ACC
BstPI         336  GGTGACC              G^GT[ACGT]ACC
Bsu36I        946  CCTCAGG              CC^T[ACGT]AGG
Eam1105I      380  GACAACAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          889  GAAGAG               CTCTTC(1/4)
Eco81I        946  CCTCAGG              CC^T[ACGT]AGG
EcoO65I       336  GGTGACC              G^GT[ACGT]ACC
EcoRI        1529  GAATTC               G^AATTC
FauI          402  GCGGG                CCCGC(4/6)
FspI          221  TGCGCA               TGC^GCA
HaeII        1035  GGCGCC               [GA]GCGC^[CT]
HgaI            4  GCGTC                GACGC(5/10)
KasI         1035  GGCGCC               G^GCGCC
Ksp632I       889  GAAGAG               CTCTTC(1/4)
MroI          315  TCCGGA               T^CCGGA
MseI         1852  TTAA                 T^TAA
NaeI          179  GCCGGC               GCC^GGC
NarI         1035  GGCGCC               GG^CGCC
NgoMIV        179  GCCGGC               G^CCGGC
PflMI        1045  CCAGAGGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI        1016  ACCGGT               A^CCGGT
SfoI         1035  GGCGCC               GGC^GCC
SmlI         1080  CTCAAG               C^T[CT][GA]AG
SphI         1760  GCATGC               GCATG^C
SspBI         671  TGTACA               T^GTACA
Tru9I        1852  TTAA                 T^TAA
Van91I       1045  CCAGAGGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          147  CCAAGAGGCAGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AcyI            3  GGCGTC               G[GA]^CG[CT]C
             1035  GGCGCC              
AfaI          672  GTAC                 GT^AC
             1814  GTAC                
BamHI         702  GGATCC               G^GATCC
              831  GGATCC              
BbsI          616  GAAGAC               GAAGAC(2/6)
             1004  GAAGAC              
BfaI          523  CTAG                 C^TAG
              707  CTAG                
BglI          866  GCCCCAGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1699  GCCCATAAGGC         
BglII         236  AGATCT               A^GATCT
             1490  AGATCT              
BpuAI         616  GAAGAC               GAAGAC(2/6)
             1004  GAAGAC              
BsaHI           3  GGCGTC               G[GA]^CG[CT]C
             1035  GGCGCC              
BsaWI         315  TCCGGA               [AT]^CCGG[AT]
             1016  ACCGGT              
BsiEI        1018  CGGTCG               CG[GA][CT]^CG
             1102  CGACCG              
BsrFI         179  GCCGGC               [GA]^CCGG[CT]
             1016  ACCGGT              
Cfr10I        179  GCCGGC               [GA]^CCGG[CT]
             1016  ACCGGT              
Hin1I           3  GGCGTC               G[GA]^CG[CT]C
             1035  GGCGCC              
HindIII      1746  AAGCTT               A^AGCTT
             1998  AAGCTT              
MaeI          523  CTAG                 C^TAG
              707  CTAG                
MaeII         662  ACGT                 A^CGT
             1257  ACGT                
MslI          801  CACCCAGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1366  CACACGGGTG          
MspA1I        155  CAGCTG               C[AC]G^C[GT]G
             1202  CAGCTG              
NspI         1197  ACATGC               [GA]CATG^[CT]
             1760  GCATGC              
PpuMI         542  GGGACCC              [GA]G^G[AT]CC[CT]
              791  AGGTCCC             
PstI          511  CTGCAG               CTGCA^G
             1114  CTGCAG              
PvuII         155  CAGCTG               CAG^CTG
             1202  CAGCTG              
RsaI          672  GTAC                 GT^AC
             1814  GTAC                
SfcI          511  CTGCAG               C^T[GA][CT]AG
             1114  CTGCAG              
SmaI           12  CCCGGG               CCC^GGG
              164  CCCGGG              
StuI          113  AGGCCT               AGG^CCT
             1064  AGGCCT              
TfiI         1868  GAATC                G^A[AT]TC
             1938  GATTC               
XmaCI          12  CCCGGG               C^CCGGG
              164  CCCGGG              
XmaI           12  CCCGGG               C^CCGGG
              164  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   79 enzymes) #
#########################################

AciI        AcsI        AluI        AlwI        AlwNI       
ApaI        ApoI        Asp700I     AvaI        AvaII       
BanI        BanII       BbvI        BcnI        BmyI        
BpmI        BsaJI       BseRI       BsgI        BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
BspHI       BsrI        BssKI       BstAPI      BstF5I      
BstNI       BstYI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraII       DsaI        EcoNI       
EcoO109I    EcoRII      EcoT14I     Fnu4HI      FokI        
HaeIII      HapII       HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        MaeIII      MboI        
MboII       MflI        MnlI        MspI        MvaI        
MwoI        NciI        NcoI        NdeII       NlaIII      
NlaIV       PleI        RcaI        Sau3AI      Sau96I      
ScrFI       SfaNI       StyI        TseI        Tsp45I      
Tsp509I     TspRI       XhoII       XmnI        
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