Restriction map for pF1KB9558
 (Linear) (Six-base) MAPSORT of: KIBB9558.seq  Check: 4106  from: 1  to: 2049



KIBB9558.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:30  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     114     623    1311    2049 
   Size:        114     509     688     738

  Fragments arranged by size: 

                 738     688     509     114
 
AgeI A'CCGG_T

Cuts at:      0    1016    2049 
   Size:       1016    1033
 
AhdI GACnn_n'nnGTC

Cuts at:      0     385    2049 
   Size:        385    1664
 
AleI CACnn'nnGTG

Cuts at:      0     805    1370    2049 
   Size:        805     565     679

  Fragments arranged by size: 

                 805     679     565
 
AlwNI CAG_nnn'CTG

Cuts at:      0      81     267     309     519     954    2049 
   Size:         81     186      42     210     435    1095

  Fragments arranged by size: 

                1095     435     210     186      81      42
 
ApaI G_GGCC'C

Cuts at:      0      13     638     720    2049 
   Size:         13     625      82    1329

  Fragments arranged by size: 

                1329     625      82      13
 
ApoI r'AATT_y

Cuts at:      0     895    1155    1529    2049 
   Size:        895     260     374     520

  Fragments arranged by size: 

                 895     520     374     260
 
AvaI C'yCGr_G

Cuts at:      0      12     109     164     971    1561    2049 
   Size:         12      97      55     807     590     488

  Fragments arranged by size: 

                 807     590     488      97      55      12
 
BamHI G'GATC_C

Cuts at:      0     702     831    2049 
   Size:        702     129    1218

  Fragments arranged by size: 

                1218     702     129
 
BanI G'GyrC_C

Cuts at:      0     799     981    1035    1184    1436    1520    1859    2049 
   Size:        799     182      54     149     252      84     339     190

  Fragments arranged by size: 

                 799     339     252     190     182     149      84      54
            
 
BanII G_rGCy'C

Cuts at:      0      13      19      38     109     638     720     868    1701 
   Size:         13       6      19      71     529      82     148     833

Cuts at:   1701    2049 
   Size:        348

  Fragments arranged by size: 

                 833     529     348     148      82      71      19      13
                   6
 
BbeI G_GCGC'C

Cuts at:      0    1039    2049 
   Size:       1039    1010
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     623    1011    2049 
   Size:        623     388    1038

  Fragments arranged by size: 

                1038     623     388
 
BbvCI CC'TCA_GC

Cuts at:      0     175    2049 
   Size:        175    1874
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1095    1129    2049 
   Size:       1095      34     920

  Fragments arranged by size: 

                1095     920      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     872    1705    2049 
   Size:        872     833     344

  Fragments arranged by size: 

                 872     833     344
 
BglII A'GATC_T

Cuts at:      0     236    1490    2049 
   Size:        236    1254     559

  Fragments arranged by size: 

                1254     559     236
 
Bme1580I G_kGCm'C

Cuts at:      0      13     638     720     986    1477    2049 
   Size:         13     625      82     266     491     572

  Fragments arranged by size: 

                 625     572     491     266      82      13
 
BmgBI CAC'GTC

Cuts at:      0     663    2049 
   Size:        663    1386
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     633     641     776     830    1105    1463    2049 
   Size:        633       8     135      54     275     358     586

  Fragments arranged by size: 

                 633     586     358     275     135      54       8
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      73      90     493     708     787     866    1008    1559 
   Size:         73      17     403     215      79      79     142     551

Cuts at:   1559    2049 
   Size:        490

  Fragments arranged by size: 

                 551     490     403     215     142      79      79      73
                  17
 
Bpu10I CC'TnA_GC

Cuts at:      0     175     862     995    2049 
   Size:        175     687     133    1054

  Fragments arranged by size: 

                1054     687     175     133
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1065    2049 
   Size:       1065     984
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1962    2049 
   Size:       1962      87
 
BsaHI Gr'CG_yC

Cuts at:      0       4    1036    2049 
   Size:          4    1032    1013

  Fragments arranged by size: 

                1032    1013       4
 
BsaWI w'CCGG_w

Cuts at:      0     315    1016    2049 
   Size:        315     701    1033

  Fragments arranged by size: 

                1033     701     315
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    2001    2031    2049 
   Size:       2001      30      18

  Fragments arranged by size: 

                2001      30      18
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      70     439     700    1306    2049 
   Size:         70     369     261     606     743

  Fragments arranged by size: 

                 743     606     369     261      70
 
BseYI C'CCAG_C

Cuts at:      0     529     876     999    2049 
   Size:        529     347     123    1050

  Fragments arranged by size: 

                1050     529     347     123
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     322     757    1215    2049 
   Size:        322     435     458     834

  Fragments arranged by size: 

                 834     458     435     322
 
BsiEI CG_ry'CG

Cuts at:      0    1021    1105    2049 
   Size:       1021      84     944

  Fragments arranged by size: 

                1021     944      84
 
BsiHKAI G_wGCw'C

Cuts at:      0    1281    2049 
   Size:       1281     768
 
Bsp1286I G_dGCh'C

Cuts at:      0      13      19      38     109     638     720     868     986 
   Size:         13       6      19      71     529      82     148     118

Cuts at:    986    1281    1477    1701    2049 
   Size:        295     196     224     348

  Fragments arranged by size: 

                 529     348     295     224     196     148     118      82
                  71      19      13       6
 
BspEI T'CCGG_A

Cuts at:      0     315    2049 
   Size:        315    1734
 
BspHI T'CATG_A

Cuts at:      0    1011    1617    1899    2049 
   Size:       1011     606     282     150

  Fragments arranged by size: 

                1011     606     282     150
 
BsrBI CCG'CTC

Cuts at:      0     323    2049 
   Size:        323    1726
 
BsrDI GCAATG_nn'

Cuts at:      0     674    2049 
   Size:        674    1375
 
BsrFI r'CCGG_y

Cuts at:      0     179    1016    2049 
   Size:        179     837    1033

  Fragments arranged by size: 

                1033     837     179
 
BsrGI T'GTAC_A

Cuts at:      0     671    2049 
   Size:        671    1378
 
BssSI C'ACGA_G

Cuts at:      0     449    2049 
   Size:        449    1600
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     309     519     574    1397    1761    2049 
   Size:        309     210      55     823     364     288

  Fragments arranged by size: 

                 823     364     309     288     210      55
 
BstEII G'GTnAC_C

Cuts at:      0     336    2049 
   Size:        336    1713
 
BstYI r'GATC_y

Cuts at:      0     236     287     702     831    1490    1843    2049 
   Size:        236      51     415     129     659     353     206

  Fragments arranged by size: 

                 659     415     353     236     206     129      51
 
Bsu36I CC'TnA_GG

Cuts at:      0     947    2049 
   Size:        947    1102
 
BtgI C'CryG_G

Cuts at:      0     198     396    1692    2049 
   Size:        198     198    1296     357

  Fragments arranged by size: 

                1296     357     198     198
 
EarI CTCTTCn'nnn_

Cuts at:      0     884    2049 
   Size:        884    1165
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      73      90     114     493     623     708     787     866 
   Size:         73      17      24     379     130      85      79      79

Cuts at:    866    1008    1311    1559    2049 
   Size:        142     303     248     490

  Fragments arranged by size: 

                 490     379     303     248     142     130      85      79
                  79      73      24      17
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      95     280     346     602    1288    2049 
   Size:         95     185      66     256     686     761

  Fragments arranged by size: 

                 761     686     256     185      95      66
 
EcoO109I rG'GnC_Cy

Cuts at:      0     526     543     635     716     717     792     858    2049 
   Size:        526      17      92      81       1      75      66    1191

  Fragments arranged by size: 

                1191     526      92      81      75      66      17       1
            
 
EcoRI G'AATT_C

Cuts at:      0    1529    2049 
   Size:       1529     520
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1737    2049 
   Size:       1737     312
 
FspI TGC'GCA

Cuts at:      0     223    2049 
   Size:        223    1826
 
FspAI rTGC'GCAy

Cuts at:      0     223    2049 
   Size:        223    1826
 
HaeII r_GCGC'y

Cuts at:      0    1039    2049 
   Size:       1039    1010
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     371     403    1482    1514    1903    1935    2001    2033 
   Size:        371      32    1079      32     389      32      66      32

Cuts at:   2033    2049 
   Size:         16

  Fragments arranged by size: 

                1079     389     371      66      32      32      32      32
                  16
 
HindIII A'AGCT_T

Cuts at:      0    1746    1998    2049 
   Size:       1746     252      51

  Fragments arranged by size: 

                1746     252      51
 
KasI G'GCGC_C

Cuts at:      0    1035    2049 
   Size:       1035    1014
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1448    1532    2049 
   Size:       1448      84     517

  Fragments arranged by size: 

                1448     517      84
 
MslI CAynn'nnrTG

Cuts at:      0     805    1370    2049 
   Size:        805     565     679

  Fragments arranged by size: 

                 805     679     565
 
MspA1I CmG'CkG

Cuts at:      0     157    1204    2049 
   Size:        157    1047     845

  Fragments arranged by size: 

                1047     845     157
 
NaeI GCC'GGC

Cuts at:      0     181    2049 
   Size:        181    1868
 
NarI GG'CG_CC

Cuts at:      0    1036    2049 
   Size:       1036    1013
 
NcoI C'CATG_G

Cuts at:      0     198     396    1692    2049 
   Size:        198     198    1296     357

  Fragments arranged by size: 

                1296     357     198     198
 
NgoMIV G'CCGG_C

Cuts at:      0     179    2049 
   Size:        179    1870
 
NspI r_CATG'y

Cuts at:      0    1201    1764    2049 
   Size:       1201     563     285

  Fragments arranged by size: 

                1201     563     285
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1051    2049 
   Size:       1051     998
 
PfoI T'CCnGG_A

Cuts at:      0     882    2049 
   Size:        882    1167
 
PpuMI rG'GwC_Cy

Cuts at:      0     543     792    2049 
   Size:        543     249    1257

  Fragments arranged by size: 

                1257     543     249
 
PsiI TTA'TAA

Cuts at:      0    1553    2049 
   Size:       1553     496
 
PspOMI G'GGCC_C

Cuts at:      0       9     634     716    2049 
   Size:          9     625      82    1333

  Fragments arranged by size: 

                1333     625      82       9
 
PstI C_TGCA'G

Cuts at:      0     515    1118    2049 
   Size:        515     603     931

  Fragments arranged by size: 

                 931     603     515
 
PvuII CAG'CTG

Cuts at:      0     157    1204    2049 
   Size:        157    1047     845

  Fragments arranged by size: 

                1047     845     157
 
SanDI GG'GwC_CC

Cuts at:      0     543    2049 
   Size:        543    1506
 
SfcI C'TryA_G

Cuts at:      0     511    1114    2049 
   Size:        511     603     935

  Fragments arranged by size: 

                 935     603     511
 
SfoI GGC'GCC

Cuts at:      0    1037    2049 
   Size:       1037    1012
 
SmaI CCC'GGG

Cuts at:      0      14     166    2049 
   Size:         14     152    1883

  Fragments arranged by size: 

                1883     152      14
 
SmlI C'TyrA_G

Cuts at:      0    1080    2049 
   Size:       1080     969
 
SphI G_CATG'C

Cuts at:      0    1764    2049 
   Size:       1764     285
 
SrfI GCCC'GGGC

Cuts at:      0      14    2049 
   Size:         14    2035
 
StuI AGG'CCT

Cuts at:      0     115    1066    2049 
   Size:        115     951     983

  Fragments arranged by size: 

                 983     951     115
 
StyI C'CwwG_G

Cuts at:      0     198     396    1067    1692    2049 
   Size:        198     198     671     625     357

  Fragments arranged by size: 

                 671     625     357     198     198
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     117     191     817    1119    1210    1354    1378    2049 
   Size:        117      74     626     302      91     144      24     671

  Fragments arranged by size: 

                 671     626     302     144     117      91      74      24
            
 
TatI w'GTAC_w

Cuts at:      0     671    2049 
   Size:        671    1378
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     398     430    2049 
   Size:        398      32    1619

  Fragments arranged by size: 

                1619     398      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     154    2049 
   Size:        154    1895
 
XmaI C'CCGG_G

Cuts at:      0      12     164    2049 
   Size:         12     152    1885

  Fragments arranged by size: 

                1885     152      12
 
XmnI GAAnn'nnTTC

Cuts at:      0     914    1324    1576    1747    2049 
   Size:        914     410     252     171     302

  Fragments arranged by size: 

                 914     410     302     252     171
 

 Enzymes that do cut:

     AcuI     AgeI     AhdI     AleI    AlwNI     ApaI     ApoI     AvaI
    BamHI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI     BglI
    BglII Bme1580I    BmgBI     BmrI     BpmI   Bpu10I    BpuEI     BsaI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
 Bsp1286I    BspEI    BspHI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI
   BstAPI   BstEII    BstYI   Bsu36I     BtgI     EarI  Eco57MI    EcoNI
 EcoO109I    EcoRI     FalI     FspI    FspAI    HaeII    Hin4I  HindIII
     KasI     MmeI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV
     NspI    PflMI     PfoI    PpuMI     PsiI   PspOMI     PstI    PvuII
    SanDI     SfcI     SfoI     SmaI     SmlI     SphI     SrfI     StuI
     StyI    TaqII     TatI     TstI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AlfI     AloI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI
    BciVI     BclI    BfrBI     BlpI     BmtI     BplI    BsaAI    BsaBI
    BsiWI     BsmI    BsmBI    BspMI   BssHII    BstBI    BstXI  BstZ17I
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EciI  EcoICRI    EcoRV     FseI   HincII     HpaI     KpnI
     MfeI     MluI     MscI     NdeI     NheI     NotI     NruI     NsiI
     PacI     PasI     PciI     PmeI     PmlI     PpiI    PshAI    PspXI
     PsrI     PvuI    RsrII     SacI    SacII     SalI     SapI     SbfI
     ScaI    SexAI     SfiI     SgfI    SgrAI    SnaBI     SpeI     SspI
     SwaI  Tth111I     XbaI     XhoI     ZraI
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