Restriction map for
pF1KB5452
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# NO CUT ENZYMES ( 161 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI Asp700I
Asp718I AspEI AspHI AspI AvaI
AvaII AviII AvrII BalI BamHI
BanI BanII BbeI BbrPI BbvCI
BcgI BclI BcnI BglI BlnI
BlpI BmyI Bpu1102I BsaBI BsaHI
BsaWI BseAI BsgI BsiEI BsiHKAI
BsiWI BsiYI BslI BsmBI BsoBI
Bsp1286I Bsp1407I BspDI BspEI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstUI BstXI BstZ17I
CelII CfoI Cfr10I Cfr13I ClaI
CpoI DraII DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO109I EcoRI EcoRV EcoT14I EcoT22I
FauI FbaI FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HindIII HpaI HpaII KasI KpnI
KspI MamI MfeI MluI MluNI
MroI MscI MslI MspA1I MspI
MunI MvnI NaeI NarI NciI
NcoI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI Psp1406I PvuI PvuII
RsrII SacI SacII SalI Sau96I
SbfI SfaNI SfiI SfoI SfuI
SgrAI SmaI SpeI SphI Sse8387I
SspBI StyI SwaI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AcsI 393 AAATTC [GA]^AATT[CT]
AflII 576 CTTAAG C^TTAAG
AflIII 761 ACATGT A^C[GA][CT]GT
AlwNI 476 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 270 CCTGAGG CC^T[ACGT]AGG
ApoI 393 AAATTC [GA]^AATT[CT]
AseI 418 ATTAAT AT^TAAT
AsnI 418 ATTAAT AT^TAAT
BciVI 998 GTATCC GTATCC(6/5)
BfrI 576 CTTAAG C^TTAAG
BsaAI 666 TACGTA [CT]AC^GT[GA]
BsaI 59 GGTCTC GGTCTC(1/5)
BsaJI 192 CCGAGG C^C[ACGT][ACGT]GG
BseRI 12 GAGGAG GAGGAG(10/8)
BsmFI 259 GTCCC GGGAC(10/14)
BsmI 48 GAATGC GAATGC(1/-1)
BspLU11I 761 ACATGT A^CATGT
BspMI 1241 GCAGGT ACCTGC(4/8)
BsrDI 524 GCAATG GCAATG(2/0)
BstEII 838 GGTTACC G^GT[ACGT]ACC
BstF5I 388 CATCC GGATG(2/0)
BstPI 838 GGTTACC G^GT[ACGT]ACC
Bsu36I 270 CCTGAGG CC^T[ACGT]AGG
Cac8I 1237 GCAGGC GC[ACGT]^[ACGT]GC
DraI 982 TTTAAA TTT^AAA
DraIII 344 CACTTAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 760 GACATGTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 500 GAAGAG CTCTTC(1/4)
Eco81I 270 CCTGAGG CC^T[ACGT]AGG
EcoO65I 838 GGTTACC G^GT[ACGT]ACC
FokI 388 CATCC GGATG(9/13)
HphI 313 TCACC GGTGA(8/7)
Ksp632I 500 GAAGAG CTCTTC(1/4)
NdeI 737 CATATG CA^TATG
NlaIV 1181 GGAACC GG[ACGT]^[ACGT]CC
PleI 1103 GAGTC GAGTC(4/5)
PshBI 418 ATTAAT AT^TAAT
PstI 473 CTGCAG CTGCA^G
SapI 500 GAAGAGC GCTCTTC(1/4)
ScaI 87 AGTACT AGT^ACT
SexAI 540 ACCTGGT A^CC[AT]GGT
SfcI 473 CTGCAG C^T[GA][CT]AG
SmlI 576 CTTAAG C^T[CT][GA]AG
SnaBI 666 TACGTA TAC^GTA
TfiI 161 GATTC G^A[AT]TC
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AciI 69 CCGC CCGC(-3/-1)
1002 CCGC
AlwI 1060 GATCC GGATC(4/5)
1034 GGATC
BbsI 17 GAAGAC GAAGAC(2/6)
928 GTCTTC
BglII 807 AGATCT A^GATCT
963 AGATCT
BpmI 334 CTGGAG CTGGAG(16/14)
959 CTGGAG
BpuAI 17 GAAGAC GAAGAC(2/6)
928 GTCTTC
BspHI 135 TCATGA T^CATGA
636 TCATGA
HaeIII 275 GGCC GG^CC
331 GGCC
HincII 204 GTCAAC GT[CT]^[GA]AC
769 GTCAAC
HindII 204 GTCAAC GT[CT]^[GA]AC
769 GTCAAC
HinfI 161 GATTC G^A[ACGT]TC
1103 GAGTC
MaeII 606 ACGT A^CGT
667 ACGT
NspI 761 ACATGT [GA]CATG^[CT]
890 ACATGC
RcaI 135 TCATGA T^CATGA
636 TCATGA
SspI 561 AATATT AAT^ATT
779 AATATT
StuI 274 AGGCCT AGG^CCT
330 AGGCCT
TaqI 867 TCGA T^CGA
1114 TCGA
TthHB8I 867 TCGA T^CGA
1114 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AfaI AluI BbvI BfaI BsmAI
BsrI BssKI BstNI BstYI DdeI
DpnII EcoRII Fnu4HI ItaI MaeI
MaeIII MboI MboII MflI MnlI
MseI MvaI MwoI NdeII NlaIII
RsaI Sau3AI ScrFI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII