Restriction map for pF1KB5451
##################################
# NO CUT ENZYMES (  128 enzymes) #
##################################

Acc65I      AccIII      AclI        AflII       AgeI        
AhdI        Alw44I      AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AviII       AvrII       
BamHI       BbrPI       BbvCI       BcgI        BciVI       
BclI        BfrI        BglII       BlnI        BlpI        
BpmI        Bpu1102I    BsaBI       BseAI       BsiEI       
BsiWI       BsmBI       BsmI        Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrBI       BsrGI       
BssHII      BssSI       Bst1107I    BstBI       BstYI       
BstZ17I     Bsu36I      CelII       ClaI        CpoI        
DraI        DraIII      DrdI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoRV       EcoT22I     FbaI        FseI        FspI        
HincII      HindII      HindIII     HpaI        KpnI        
KspI        MamI        MfeI        MflI        MluI        
MroI        MunI        NcoI        NdeI        NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PflFI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        RcaI        RsrII       SacII       
SalI        SbfI        ScaI        SexAI       SfcI        
SfiI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        Tth111I     XcmI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AatII         465  GACGTC               GACGT^C
AccI          139  GTCTAC               GT^[AC][GT]AC
AflIII       1043  ACGTGT               A^C[GA][CT]GT
AspHI         268  GAGCTC               G[AT]GC[AT]^C
AvaII         356  GGACC                G^G[AT]CC
BanI          130  GGCGCC               G^G[CT][GA]CC
BbeI          130  GGCGCC               GGCGC^C
BbsI          558  GTCTTC               GAAGAC(2/6)
BcnI          221  CCCGG                CC^[GC]GG
BglI          614  GCCATATTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI         558  GTCTTC               GAAGAC(2/6)
BsaAI        1084  TACGTG               [CT]AC^GT[GA]
BsaI          874  GAGACC               GGTCTC(1/5)
BsaWI         116  ACCGGA               [AT]^CCGG[AT]
BseRI         265  GAGGAG               GAGGAG(10/8)
BsgI         1087  GTGCAG               GTGCAG(16/14)
BsiHKAI       268  GAGCTC               G[AT]GC[AT]^C
BspMI          55  GCAGGT               ACCTGC(4/8)
BsrDI         194  GCAATG               GCAATG(2/0)
BsrFI         190  GCCGGC               [GA]^CCGG[CT]
BstAPI        950  GCAGAAACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        984  GGTGACC              G^GT[ACGT]ACC
BstPI         984  GGTGACC              G^GT[ACGT]ACC
Cfr10I        190  GCCGGC               [GA]^CCGG[CT]
DraII         756  AGGCCCT              [GA]G^G[ACGT]CC[CT]
DsaI          460  CCACGG               C^C[GA][CT]GG
EcoO109I      756  AGGCCCT              [GA]G^G[ACGT]CC[CT]
EcoO65I       984  GGTGACC              G^GT[ACGT]ACC
EcoRI         323  GAATTC               G^AATTC
FauI          933  CCCGC                CCCGC(4/6)
HaeII         130  GGCGCC               [GA]GCGC^[CT]
KasI          130  GGCGCC               G^GCGCC
MseI          595  TTAA                 T^TAA
NaeI          190  GCCGGC               GCC^GGC
NarI          130  GGCGCC               GG^CGCC
NciI          221  CCCGG                CC^[GC]GG
NgoMIV        190  GCCGGC               G^CCGGC
PaeR7I       1020  CTCGAG               C^TCGAG
PflMI         535  CCAGGGGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII         277  CAGCTG               CAG^CTG
SacI          268  GAGCTC               GAGCT^C
SapI         1056  GCTCTTC              GCTCTTC(1/4)
SfoI          130  GGCGCC               GGC^GCC
SmlI         1020  CTCGAG               C^T[CT][GA]AG
TfiI          545  GATTC                G^A[AT]TC
Tru9I         595  TTAA                 T^TAA
Van91I        535  CCAGGGGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI          180  TCTAGA               T^CTAGA
XhoI         1020  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AlwI          378  GATCC                GGATC(4/5)
              119  GGATC               
AlwNI         583  CAGAAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              951  CAGAAACTG           
AvaI          231  CCCGAG               C^[CT]CG[GA]G
             1020  CTCGAG              
BalI          341  TGGCCA               TGG^CCA
             1097  TGGCCA              
BanII         268  GAGCTC               G[GA]GC[CT]^C
              843  GGGCTC              
BfaI           50  CTAG                 C^TAG
              181  CTAG                
BmyI          268  GAGCTC               G[AGT]GC[ACT]^C
              843  GGGCTC              
BsmAI         234  GAGAC                GTCTC(1/5)
              874  GAGAC               
BsoBI         231  CCCGAG               C^[CT]CG[GA]G
             1020  CTCGAG              
Bsp1286I      268  GAGCTC               G[AGT]GC[ACT]^C
              843  GGGCTC              
BstXI         626  CCATGAGAGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              789  CCAATCCAGTGG        
CfoI          131  GCGC                 GCG^C
             1025  GCGC                
DpnII         120  GATC                 ^GATC
              378  GATC                
EarI         1057  CTCTTC               CTCTTC(1/4)
              871  GAAGAG              
HhaI          131  GCGC                 GCG^C
             1025  GCGC                
HinP1I        131  GCGC                 G^CGC
             1025  GCGC                
Ksp632I      1057  CTCTTC               CTCTTC(1/4)
              871  GAAGAG              
MaeI           50  CTAG                 C^TAG
              181  CTAG                
MboI          120  GATC                 ^GATC
              378  GATC                
MluNI         341  TGGCCA               TGG^CCA
             1097  TGGCCA              
MscI          341  TGGCCA               TGG^CCA
             1097  TGGCCA              
NdeII         120  GATC                 ^GATC
              378  GATC                
NlaIII        144  CATG                 CATG^
              627  CATG                
PleI          800  GACTC                GAGTC(4/5)
             1018  GACTC               
Sau3AI        120  GATC                 ^GATC
              378  GATC                


#########################################
# 3 OR MORE CUT ENZYMES (   56 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AluI        ApoI        BbvI        BsaHI       BsaJI       
BsiYI       BslI        BsmFI       BsrI        BssKI       
BstF5I      BstNI       BstUI       Cac8I       Cfr13I      
DdeI        EaeI        EcoRII      EcoT14I     Fnu4HI      
FokI        HaeIII      HapII       HgaI        Hin1I       
HinfI       HpaII       HphI        ItaI        MaeII       
MaeIII      MboII       MnlI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NlaIV       
RsaI        Sau96I      ScrFI       SfaNI       StyI        
TaqI        TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     
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