Restriction map for pF1KB5422
##################################
# NO CUT ENZYMES (  145 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaLI       AscI        AseI        
AsnI        Asp718I     AspEI       AspHI       AspI        
AviII       AvrII       BalI        BamHI       BanI        
BbeI        BbsI        BbvCI       BciVI       BclI        
BfaI        BfrI        BglI        BlnI        BlpI        
Bpu1102I    BpuAI       BsaBI       BsaWI       BseAI       
BseRI       BsiEI       BsiHKAI     BsiWI       BsmBI       
BsmI        Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrGI       
BssHII      Bst1107I    BstAPI      BstBI       BstXI       
BstZ17I     Bsu36I      CelII       ClaI        CpoI        
DraI        DraIII      DrdI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoRV       
EcoT14I     EcoT22I     FbaI        FseI        FspI        
HaeII       HincII      HindII      HindIII     HinfI       
HpaI        KasI        KpnI        KspI        MaeI        
MamI        MfeI        MluI        MluNI       MroI        
MscI        MseI        MunI        NarI        NcoI        
NdeI        NheI        NlaIII      NotI        NruI        
NsiI        NspI        NspV        PacI        PflFI       
PinAI       PleI        PmeI        PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacI        
SacII       SalI        SbfI        ScaI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        StyI        SwaI        TfiI        Tru9I       
Tth111I     XbaI        XcmI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AcsI          346  GAATTC               [GA]^AATT[CT]
AcyI          530  GGCGTC               G[GA]^CG[CT]C
AlwI          321  GGATC                GGATC(4/5)
ApaI          626  GGGCCC               GGGCC^C
ApoI          346  GAATTC               [GA]^AATT[CT]
BbrPI          58  CACGTG               CAC^GTG
BcgI          615  GCATCACCATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII         206  AGATCT               A^GATCT
BsaAI          58  CACGTG               [CT]AC^GT[GA]
BsaHI         530  GGCGTC               G[GA]^CG[CT]C
BsrFI         581  GCCGGC               [GA]^CCGG[CT]
BssSI         127  CTCGTG               CACGAG(-5/-1)
BstEII        601  GGTCACC              G^GT[ACGT]ACC
BstPI         601  GGTCACC              G^GT[ACGT]ACC
Cfr10I        581  GCCGGC               [GA]^CCGG[CT]
DsaI          191  CCGTGG               C^C[GA][CT]GG
EaeI          194  TGGCCG               [CT]^GGCC[GA]
EcoNI         153  CCTGCGAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       601  GGTCACC              G^GT[ACGT]ACC
EcoRI         346  GAATTC               G^AATTC
HgaI          531  GCGTC                GACGC(5/10)
Hin1I         530  GGCGTC               G[GA]^CG[CT]C
MaeII          59  ACGT                 A^CGT
MslI           54  CACGCACGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI          581  GCCGGC               GCC^GGC
NgoMIV        581  GCCGGC               G^CCGGC
PaeR7I        329  CTCGAG               C^TCGAG
PflMI         186  CCAGCCCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI          58  CACGTG               CAC^GTG
PmlI           58  CACGTG               CAC^GTG
PstI           99  CTGCAG               CTGCA^G
SapI          475  GAAGAGC              GCTCTTC(1/4)
SexAI         359  ACCAGGT              A^CC[AT]GGT
SfcI           99  CTGCAG               C^T[GA][CT]AG
SmlI          329  CTCGAG               C^T[CT][GA]AG
Tsp509I       347  AATT                 ^AATT
TspRI         171  CACTG                CA[GC]TG[ACGT][ACGT]^
Van91I        186  CCAGCCCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI          329  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

AccII         424  CGCG                 CG^CG
              430  CGCG                
AfaI          394  GTAC                 GT^AC
              589  GTAC                
Asp700I       204  GAAGATCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              342  GAAGGAATTC          
BanII         626  GGGCCC               G[GA]GC[CT]^C
              652  GAGCCC              
BcnI          457  CCCGG                CC^[GC]GG
              527  CCCGG               
BpmI           43  CTGGAG               CTGGAG(16/14)
              279  CTGGAG              
BsaI          332  GAGACC               GGTCTC(1/5)
              499  GGTCTC              
BsaJI         191  CCGTGG               C^C[ACGT][ACGT]GG
              596  CCGAGG              
BsgI          229  CTGCAC               GTGCAG(16/14)
              244  CTGCAC              
BsmAI         332  GAGAC                GTCTC(1/5)
              500  GTCTC               
BstUI         424  CGCG                 CG^CG
              430  CGCG                
BstYI         206  AGATCT               [GA]^GATC[CT]
              321  GGATCT              
DdeI          173  CTGAG                C^T[ACGT]AG
              650  CTGAG               
DpnII         207  GATC                 ^GATC
              322  GATC                
EarI          262  GAAGAG               CTCTTC(1/4)
              475  GAAGAG              
HphI          603  TCACC                GGTGA(8/7)
              618  TCACC               
Ksp632I       262  GAAGAG               CTCTTC(1/4)
              475  GAAGAG              
MaeIII        130  GTGAC                ^GT[ACGT]AC
              602  GTCAC               
MboI          207  GATC                 ^GATC
              322  GATC                
MflI          206  AGATCT               [GA]^GATC[CT]
              321  GGATCT              
MvnI          424  CGCG                 CG^CG
              430  CGCG                
NciI          457  CCCGG                CC^[GC]GG
              527  CCCGG               
NdeII         207  GATC                 ^GATC
              322  GATC                
PpuMI         113  GGGACCT              [GA]G^G[AT]CC[CT]
              440  GGGACCT             
PvuII          73  CAGCTG               CAG^CTG
              398  CAGCTG              
RsaI          394  GTAC                 GT^AC
              589  GTAC                
Sau3AI        207  GATC                 ^GATC
              322  GATC                
SfaNI         482  GATGC                GCATC(5/9)
              615  GCATC               
Tsp45I        130  GTGAC                ^GT[GC]AC
              602  GTCAC               
XhoII         206  AGATCT               [GA]^GATC[CT]
              321  GGATCT              
XmnI          204  GAAGATCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              342  GAAGGAATTC          


#########################################
# 3 OR MORE CUT ENZYMES (   43 enzymes) #
#########################################

AciI        AluI        AlwNI       AvaI        AvaII       
BbvI        BmyI        BsiYI       BslI        BsmFI       
BsoBI       Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       Cac8I       CfoI        Cfr13I      DraII       
EcoO109I    EcoRII      FauI        Fnu4HI      FokI        
HaeIII      HapII       HhaI        HinP1I      HpaII       
ItaI        MboII       MnlI        MspA1I      MspI        
MvaI        MwoI        NlaIV       Sau96I      ScrFI       
TaqI        TseI        TthHB8I     
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