Restriction map for pF1KB5422
 (Linear) (Six-base) MAPSORT of: KIBB5422.seq  Check: 1994  from: 1  to: 672



KIBB5422.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:16  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     281     672 
   Size:        281     391
 
AleI CACnn'nnGTG

Cuts at:      0      58     672 
   Size:         58     614
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     169     672 
   Size:        169     503
 
AlwNI CAG_nnn'CTG

Cuts at:      0      78     107     415     672 
   Size:         78      29     308     257

  Fragments arranged by size: 

                 308     257      78      29
 
ApaI G_GGCC'C

Cuts at:      0     630     672 
   Size:        630      42
 
ApoI r'AATT_y

Cuts at:      0     346     672 
   Size:        346     326
 
AvaI C'yCGr_G

Cuts at:      0      28     329     595     672 
   Size:         28     301     266      77

  Fragments arranged by size: 

                 301     266      77      28
 
BanII G_rGCy'C

Cuts at:      0     630     656     672 
   Size:        630      26      16

  Fragments arranged by size: 

                 630      26      16
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     604     638     672 
   Size:        604      34      34

  Fragments arranged by size: 

                 604      34      34
 
BglII A'GATC_T

Cuts at:      0     206     672 
   Size:        206     466
 
Bme1580I G_kGCm'C

Cuts at:      0      65     630     672 
   Size:         65     565      42

  Fragments arranged by size: 

                 565      65      42
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     189     586     672 
   Size:        189     397      86

  Fragments arranged by size: 

                 397     189      86
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     342     672 
   Size:        342     330
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      64     300     672 
   Size:         64     236     372

  Fragments arranged by size: 

                 372     236      64
 
Bpu10I CC'TnA_GC

Cuts at:      0     650     672 
   Size:        650      22
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     326     505     672 
   Size:        326     179     167

  Fragments arranged by size: 

                 326     179     167
 
BsaAI yAC'GTr

Cuts at:      0      60     672 
   Size:         60     612
 
BsaHI Gr'CG_yC

Cuts at:      0     531     672 
   Size:        531     141
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     497     527     672 
   Size:        497      30     145

  Fragments arranged by size: 

                 497     145      30
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     214     229     672 
   Size:        214      15     443

  Fragments arranged by size: 

                 443     214      15
 
Bsp1286I G_dGCh'C

Cuts at:      0      65     630     656     672 
   Size:         65     565      26      16

  Fragments arranged by size: 

                 565      65      26      16
 
BsrFI r'CCGG_y

Cuts at:      0     581     672 
   Size:        581      91
 
BssSI C'ACGA_G

Cuts at:      0     127     672 
   Size:        127     545
 
BstEII G'GTnAC_C

Cuts at:      0     601     672 
   Size:        601      71
 
BstYI r'GATC_y

Cuts at:      0     206     321     672 
   Size:        206     115     351

  Fragments arranged by size: 

                 351     206     115
 
BtgI C'CryG_G

Cuts at:      0     191     672 
   Size:        191     481
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     317     495     672 
   Size:        317     178     177

  Fragments arranged by size: 

                 317     178     177
 
EaeI y'GGCC_r

Cuts at:      0     194     672 
   Size:        194     478
 
EarI CTCTTCn'nnn_

Cuts at:      0     257     470     672 
   Size:        257     213     202

  Fragments arranged by size: 

                 257     213     202
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      64     281     300     672 
   Size:         64     217      19     372

  Fragments arranged by size: 

                 372     217      64      19
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     157     672 
   Size:        157     515
 
EcoO109I rG'GnC_Cy

Cuts at:      0     114     441     627     672 
   Size:        114     327     186      45

  Fragments arranged by size: 

                 327     186     114      45
 
EcoRI G'AATT_C

Cuts at:      0     346     672 
   Size:        346     326
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     371     403     643     672 
   Size:        371      32     240      29

  Fragments arranged by size: 

                 371     240      32      29
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     540     672 
   Size:        540     132
 
MslI CAynn'nnrTG

Cuts at:      0      58     672 
   Size:         58     614
 
MspA1I CmG'CkG

Cuts at:      0      75     400     634     672 
   Size:         75     325     234      38

  Fragments arranged by size: 

                 325     234      75      38
 
NaeI GCC'GGC

Cuts at:      0     583     672 
   Size:        583      89
 
NgoMIV G'CCGG_C

Cuts at:      0     581     672 
   Size:        581      91
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     192     672 
   Size:        192     480
 
PmlI CAC'GTG

Cuts at:      0      60     672 
   Size:         60     612
 
PpuMI rG'GwC_Cy

Cuts at:      0     114     441     672 
   Size:        114     327     231

  Fragments arranged by size: 

                 327     231     114
 
PspOMI G'GGCC_C

Cuts at:      0     626     672 
   Size:        626      46
 
PstI C_TGCA'G

Cuts at:      0     103     672 
   Size:        103     569
 
PvuII CAG'CTG

Cuts at:      0      75     400     672 
   Size:         75     325     272

  Fragments arranged by size: 

                 325     272      75
 
SapI GCTCTTCn'nnn_

Cuts at:      0     470     672 
   Size:        470     202
 
SexAI A'CCwGG_T

Cuts at:      0     359     672 
   Size:        359     313
 
SfcI C'TryA_G

Cuts at:      0      99     672 
   Size:         99     573
 
SmlI C'TyrA_G

Cuts at:      0     329     672 
   Size:        329     343
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     497     529     672 
   Size:        497      32     143

  Fragments arranged by size: 

                 497     143      32
 
XhoI C'TCGA_G

Cuts at:      0     329     672 
   Size:        329     343
 
XmnI GAAnn'nnTTC

Cuts at:      0     208     346     672 
   Size:        208     138     326

  Fragments arranged by size: 

                 326     208     138
 

 Enzymes that do cut:

     AcuI     AleI     AlfI    AlwNI     ApaI     ApoI     AvaI    BanII
     BcgI    BglII Bme1580I     BmrI     BplI     BpmI   Bpu10I     BsaI
    BsaAI    BsaHI    BsaXI     BsgI Bsp1286I    BsrFI    BssSI   BstEII
    BstYI     BtgI    BtgZI     EaeI     EarI  Eco57MI    EcoNI EcoO109I
    EcoRI    Hin4I     MmeI     MslI   MspA1I     NaeI   NgoMIV    PflMI
     PmlI    PpuMI   PspOMI     PstI    PvuII     SapI    SexAI     SfcI
     SmlI     TstI     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AloI    ApaLI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BanI     BbeI     BbsI    BbvCI    BciVI     BclI
    BfrBI     BglI     BlpI    BmgBI     BmtI    BpuEI    BsaBI    BsaWI
    BseRI    BseYI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrGI   BssHII   BstAPI    BstBI
    BstXI  BstZ17I   Bsu36I     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EagI     EciI  EcoICRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MscI     NarI     NcoI     NdeI     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI     PfoI     PmeI     PpiI
    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI    TaqII     TatI  Tth111I     XbaI     XcmI     XmaI     ZraI
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