Restriction map for pF1KB5361
##################################
# NO CUT ENZYMES (  110 enzymes) #
##################################

AatII       AccI        AclI        AcsI        AflII       
AflIII      AgeI        AocI        Aor51HI     ApaI        
ApoI        AscI        AseI        AsnI        AspI        
AviII       AvrII       BanII       BbeI        BbrPI       
BbvCI       BcgI        BciVI       BclI        BfrI        
BlnI        BsaAI       BsiEI       BsiWI       BsmBI       
BsmFI       Bsp1407I    BspDI       BspHI       BspLU11I    
BsrGI       BssHII      BssSI       Bst1107I    BstBI       
BstEII      BstPI       BstZ17I     Bsu36I      ClaI        
CpoI        DraI        DraII       DraIII      EagI        
EarI        EclXI       Eco47III    Eco52I      Eco81I      
EcoO109I    EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        HindIII     KasI        
Ksp632I     KspI        MfeI        MluI        MunI        
NarI        NdeI        NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacI        SacII       SalI        SapI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SnaBI       SpeI        SspBI       SspI        StuI        
SwaI        Tsp45I      Tth111I     XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   78 enzymes) #
###################################

Acc65I         32  GGTACC               G^GTACC
AccII         107  CGCG                 CG^CG
AccIII        744  TCCGGA               T^CCGGA
AcyI          825  GACGCC               G[GA]^CG[CT]C
AfaI           33  GTAC                 GT^AC
AhdI         1263  GACTCTCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I        178  GTGCAC               G^TGCAC
ApaLI         178  GTGCAC               G^TGCAC
Asp718I        32  GGTACC               G^GTACC
AspEI        1263  GACTCTCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI         178  GTGCAC               G[AT]GC[AT]^C
AvaI          901  CCCGGG               C^[CT]CG[GA]G
BamHI         817  GGATCC               G^GATCC
BanI           32  GGTACC               G^G[CT][GA]CC
BbsI          502  GAAGAC               GAAGAC(2/6)
BfaI          619  CTAG                 C^TAG
BglI          991  GCCCCCATGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         633  AGATCT               A^GATCT
BmyI          178  GTGCAC               G[AGT]GC[ACT]^C
BpmI           61  CTGGAG               CTGGAG(16/14)
BpuAI         502  GAAGAC               GAAGAC(2/6)
BsaBI         628  GATTTAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI         825  GACGCC               G[GA]^CG[CT]C
BsaI          295  GAGACC               GGTCTC(1/5)
BsaWI         744  TCCGGA               [AT]^CCGG[AT]
BseAI         744  TCCGGA               T^CCGGA
BseRI          64  GAGGAG               GAGGAG(10/8)
BsgI           83  GTGCAG               GTGCAG(16/14)
BsiHKAI       178  GTGCAC               G[AT]GC[AT]^C
BsmI          143  GCATTC               GAATGC(1/-1)
BsoBI         901  CCCGGG               C^[CT]CG[GA]G
Bsp1286I      178  GTGCAC               G[AGT]GC[ACT]^C
BspEI         744  TCCGGA               T^CCGGA
BspMI          53  ACCTGC               ACCTGC(4/8)
BsrBI         863  CCGCTC               CCGCTC(-3/-3)
BsrFI         712  GCCGGC               [GA]^CCGG[CT]
BstAPI        703  GCAGGGAGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI         107  CGCG                 CG^CG
BstXI        1071  CCAGCAGGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI          544  GCGC                 GCG^C
Cfr10I        712  GCCGGC               [GA]^CCGG[CT]
DrdI            3  GACAGGCAAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI          995  CCATGG               C^C[GA][CT]GG
Eam1105I     1263  GACTCTCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI         618  CCTAGAGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I       995  CCATGG               C^C[AT][AT]GG
FauI          862  CCCGC                CCCGC(4/6)
HaeII         543  AGCGCC               [GA]GCGC^[CT]
HgaI          825  GACGC                GACGC(5/10)
HhaI          544  GCGC                 GCG^C
Hin1I         825  GACGCC               G[GA]^CG[CT]C
HinP1I        544  GCGC                 G^CGC
HincII        202  GTTAAC               GT[CT]^[GA]AC
HindII        202  GTTAAC               GT[CT]^[GA]AC
HpaI          202  GTTAAC               GTT^AAC
KpnI           32  GGTACC               GGTAC^C
MaeI          619  CTAG                 C^TAG
MaeII          23  ACGT                 A^CGT
MaeIII        907  GTTAC                ^GT[ACGT]AC
MamI          628  GATTTAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI          744  TCCGGA               T^CCGGA
MseI          203  TTAA                 T^TAA
MvnI          107  CGCG                 CG^CG
NaeI          712  GCCGGC               GCC^GGC
NcoI          995  CCATGG               C^CATGG
NgoMIV        712  GCCGGC               G^CCGGC
PflMI        1170  CCAGCAGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI         373  GACCGAAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsaI           33  GTAC                 GT^AC
SbfI         1006  CCTGCAGG             CCTGCA^GG
SmaI          901  CCCGGG               CCC^GGG
Sse8387I     1006  CCTGCAGG             CCTGCA^GG
StyI          995  CCATGG               C^C[AT][AT]GG
Tru9I         203  TTAA                 T^TAA
Tsp509I       455  AATT                 ^AATT
Van91I       1170  CCAGCAGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI         901  CCCGGG               C^CCGGG
XmaI          901  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AlwNI         542  CAGCGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1138  CAGCAGCTG           
Asp700I       226  GAACAGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              436  GAACCAGTTC          
AvaII         372  GGACC                G^G[AT]CC
             1125  GGTCC               
BalI           47  TGGCCA               TGG^CCA
              998  TGGCCA              
BcnI          901  CCCGG                CC^[GC]GG
              902  CCGGG               
BlpI          964  GCTCAGC              GC^T[ACGT]AGC
             1135  GCTCAGC             
Bpu1102I      964  GCTCAGC              GC^T[ACGT]AGC
             1135  GCTCAGC             
BsmAI         295  GAGAC                GTCTC(1/5)
              344  GAGAC               
BstYI         633  AGATCT               [GA]^GATC[CT]
              817  GGATCC              
CelII         964  GCTCAGC              GC^T[ACGT]AGC
             1135  GCTCAGC             
EaeI           47  TGGCCA               [CT]^GGCC[GA]
              998  TGGCCA              
HphI           15  GGTGA                GGTGA(8/7)
              480  GGTGA               
MflI          633  AGATCT               [GA]^GATC[CT]
              817  GGATCC              
MluNI          47  TGGCCA               TGG^CCA
              998  TGGCCA              
MscI           47  TGGCCA               TGG^CCA
              998  TGGCCA              
MslI          126  CATTGGTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              996  CATGGCCATG          
MspA1I        323  CAGCTG               C[AC]G^C[GT]G
             1141  CAGCTG              
NciI          901  CCCGG                CC^[GC]GG
              902  CCGGG               
NspI          239  GCATGC               [GA]CATG^[CT]
             1025  GCATGC              
PstI          701  CTGCAG               CTGCA^G
             1007  CTGCAG              
PvuII         323  CAGCTG               CAG^CTG
             1141  CAGCTG              
SfcI          701  CTGCAG               C^T[GA][CT]AG
             1007  CTGCAG              
SmlI          221  CTCAAG               C^T[CT][GA]AG
              842  CTCAAG              
SphI          239  GCATGC               GCATG^C
             1025  GCATGC              
XhoII         633  AGATCT               [GA]^GATC[CT]
              817  GGATCC              
XmnI          226  GAACAGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              436  GAACCAGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   44 enzymes) #
#########################################

AciI        AluI        AlwI        BbvI        BsaJI       
BsiYI       BslI        BsrDI       BsrI        BssKI       
BstF5I      BstNI       Cac8I       Cfr13I      DdeI        
DpnII       EcoRII      Fnu4HI      FokI        HaeIII      
HapII       HinfI       HpaII       ItaI        MboI        
MboII       MnlI        MspI        MvaI        MwoI        
NdeII       NlaIII      NlaIV       PleI        Sau3AI      
Sau96I      ScrFI       SfaNI       TaqI        TfiI        
TseI        TspRI       TthHB8I     XcmI        
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