Restriction map for pF1KB5361
 (Linear) (Six-base) MAPSORT of: KIBB5361.seq  Check: 4952  from: 1  to: 1287



KIBB5361.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:16  ..

Acc65I G'GTAC_C

Cuts at:      0      32    1287 
   Size:         32    1255
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1268    1287 
   Size:       1268      19
 
AlwNI CAG_nnn'CTG

Cuts at:      0     547    1143    1287 
   Size:        547     596     144

  Fragments arranged by size: 

                 596     547     144
 
ApaLI G'TGCA_C

Cuts at:      0     178    1287 
   Size:        178    1109
 
AvaI C'yCGr_G

Cuts at:      0     901    1287 
   Size:        901     386
 
BamHI G'GATC_C

Cuts at:      0     817    1287 
   Size:        817     470
 
BanI G'GyrC_C

Cuts at:      0      32    1287 
   Size:         32    1255
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     509    1287 
   Size:        509     778
 
BglI GCCn_nnn'nGGC

Cuts at:      0     997    1287 
   Size:        997     290
 
BglII A'GATC_T

Cuts at:      0     633    1287 
   Size:        633     654
 
BlpI GC'TnA_GC

Cuts at:      0     965    1136    1287 
   Size:        965     171     151

  Fragments arranged by size: 

                 965     171     151
 
Bme1580I G_kGCm'C

Cuts at:      0     182    1287 
   Size:        182    1105
 
BmgBI CAC'GTC

Cuts at:      0      24    1287 
   Size:         24    1263
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      82    1287 
   Size:         82    1205
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     206     827    1287 
   Size:        206     621     460

  Fragments arranged by size: 

                 621     460     206
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     289    1287 
   Size:        289     998
 
BsaBI GATnn'nnATC

Cuts at:      0     632    1287 
   Size:        632     655
 
BsaHI Gr'CG_yC

Cuts at:      0     826    1287 
   Size:        826     461
 
BsaWI w'CCGG_w

Cuts at:      0     744    1287 
   Size:        744     543
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      79    1287 
   Size:         79    1208
 
BseYI C'CCAG_C

Cuts at:      0     321     892    1070    1169    1180    1287 
   Size:        321     571     178      99      11     107

  Fragments arranged by size: 

                 571     321     178     107      99      11
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     104    1287 
   Size:        104    1183
 
BsiHKAI G_wGCw'C

Cuts at:      0     182    1287 
   Size:        182    1105
 
BsmI GAATG_Cn'

Cuts at:      0     143    1287 
   Size:        143    1144
 
Bsp1286I G_dGCh'C

Cuts at:      0     182    1287 
   Size:        182    1105
 
BspEI T'CCGG_A

Cuts at:      0     744    1287 
   Size:        744     543
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      62    1287 
   Size:         62    1225
 
BsrBI CCG'CTC

Cuts at:      0     865    1287 
   Size:        865     422
 
BsrDI GCAATG_nn'

Cuts at:      0     590     854    1153    1287 
   Size:        590     264     299     134

  Fragments arranged by size: 

                 590     299     264     134
 
BsrFI r'CCGG_y

Cuts at:      0     712    1287 
   Size:        712     575
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     709    1287 
   Size:        709     578
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1078    1287 
   Size:       1078     209
 
BstYI r'GATC_y

Cuts at:      0     633     817    1287 
   Size:        633     184     470

  Fragments arranged by size: 

                 633     470     184
 
BtgI C'CryG_G

Cuts at:      0     995    1287 
   Size:        995     292
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     123    1287 
   Size:        123    1164
 
DrdI GACnn_nn'nnGTC

Cuts at:      0       9    1287 
   Size:          9    1278
 
EaeI y'GGCC_r

Cuts at:      0      47     998    1287 
   Size:         47     951     289

  Fragments arranged by size: 

                 951     289      47
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1253    1287 
   Size:       1253      34
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      82    1287 
   Size:         82    1205
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     622    1287 
   Size:        622     665
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     304    1287 
   Size:        304     983
 
HaeII r_GCGC'y

Cuts at:      0     547    1287 
   Size:        547     740
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      79     111     861     893    1254    1286    1287 
   Size:         79      32     750      32     361      32       1

  Fragments arranged by size: 

                 750     361      79      32      32      32       1
 
HincII GTy'rAC

Cuts at:      0     204    1287 
   Size:        204    1083
 
HpaI GTT'AAC

Cuts at:      0     204    1287 
   Size:        204    1083
 
KpnI G_GTAC'C

Cuts at:      0      36    1287 
   Size:         36    1251
 
MscI TGG'CCA

Cuts at:      0      49    1000    1287 
   Size:         49     951     287

  Fragments arranged by size: 

                 951     287      49
 
MslI CAynn'nnrTG

Cuts at:      0     130    1000    1287 
   Size:        130     870     287

  Fragments arranged by size: 

                 870     287     130
 
MspA1I CmG'CkG

Cuts at:      0     325    1143    1287 
   Size:        325     818     144

  Fragments arranged by size: 

                 818     325     144
 
NaeI GCC'GGC

Cuts at:      0     714    1287 
   Size:        714     573
 
NcoI C'CATG_G

Cuts at:      0     995    1287 
   Size:        995     292
 
NgoMIV G'CCGG_C

Cuts at:      0     712    1287 
   Size:        712     575
 
NspI r_CATG'y

Cuts at:      0     243    1029    1287 
   Size:        243     786     258

  Fragments arranged by size: 

                 786     258     243
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1176    1287 
   Size:       1176     111
 
PshAI GACnn'nnGTC

Cuts at:      0     377    1287 
   Size:        377     910
 
PstI C_TGCA'G

Cuts at:      0     705    1011    1287 
   Size:        705     306     276

  Fragments arranged by size: 

                 705     306     276
 
PvuII CAG'CTG

Cuts at:      0     325    1143    1287 
   Size:        325     818     144

  Fragments arranged by size: 

                 818     325     144
 
SbfI CC_TGCA'GG

Cuts at:      0    1011    1287 
   Size:       1011     276
 
SfcI C'TryA_G

Cuts at:      0     701    1007    1287 
   Size:        701     306     280

  Fragments arranged by size: 

                 701     306     280
 
SmaI CCC'GGG

Cuts at:      0     903    1287 
   Size:        903     384
 
SmlI C'TyrA_G

Cuts at:      0     221     842    1287 
   Size:        221     621     445

  Fragments arranged by size: 

                 621     445     221
 
SphI G_CATG'C

Cuts at:      0     243    1029    1287 
   Size:        243     786     258

  Fragments arranged by size: 

                 786     258     243
 
StyI C'CwwG_G

Cuts at:      0     995    1287 
   Size:        995     292
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     389    1009    1033    1084    1287 
   Size:        389     620      24      51     203

  Fragments arranged by size: 

                 620     389     203      51      24
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     114     146    1287 
   Size:        114      32    1141

  Fragments arranged by size: 

                1141     114      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      57    1117    1177    1287 
   Size:         57    1060      60     110

  Fragments arranged by size: 

                1060     110      60      57
 
XmaI C'CCGG_G

Cuts at:      0     901    1287 
   Size:        901     386
 
XmnI GAAnn'nnTTC

Cuts at:      0     230     440    1287 
   Size:        230     210     847

  Fragments arranged by size: 

                 847     230     210
 

 Enzymes that do cut:

   Acc65I     AhdI    AlwNI    ApaLI     AvaI    BamHI     BanI     BbsI
     BglI    BglII     BlpI Bme1580I    BmgBI     BpmI    BpuEI     BsaI
    BsaBI    BsaHI    BsaWI    BseRI    BseYI     BsgI  BsiHKAI     BsmI
 Bsp1286I    BspEI    BspMI    BsrBI    BsrDI    BsrFI   BstAPI    BstXI
    BstYI     BtgI    BtgZI     DrdI     EaeI     EciI  Eco57MI    EcoNI
     FalI    HaeII    Hin4I   HincII     HpaI     KpnI     MscI     MslI
   MspA1I     NaeI     NcoI   NgoMIV     NspI    PflMI    PshAI     PstI
    PvuII     SbfI     SfcI     SmaI     SmlI     SphI     StyI    TaqII
     TstI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AleI     AlfI     AloI     ApaI     ApoI     AscI     AseI
    AsiSI    AvrII     BaeI    BanII     BbeI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BmrI     BmtI     BplI   Bpu10I    BsaAI    BsaXI
    BsiEI    BsiWI    BsmBI    BspHI    BsrGI   BssHII    BssSI    BstBI
   BstEII  BstZ17I   Bsu36I     BtsI     ClaI    CspCI     DraI   DraIII
     EagI     EarI  EcoICRI EcoO109I    EcoRI    EcoRV     FseI     FspI
    FspAI  HindIII     KasI     MfeI     MluI     MmeI     NarI     NdeI
     NheI     NotI     NruI     NsiI     PacI     PasI     PciI     PfoI
     PmeI     PmlI     PpiI    PpuMI     PsiI   PspOMI    PspXI     PsrI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI     SrfI
     SspI     StuI     SwaI     TatI  Tth111I     XbaI     XhoI     ZraI
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