Restriction map for pF1KB5272
##################################
# NO CUT ENZYMES (  156 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      AocI        Aor51HI     
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspI        AviII       
AvrII       BanI        BbeI        BbrPI       BbsI        
BcgI        BciVI       BfaI        BfrI        BglI        
BglII       BlnI        BpuAI       BsaAI       BsaBI       
BsaHI       BsaWI       BseAI       BsgI        BsiEI       
BsiWI       BsmBI       BsmFI       BsmI        BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrFI       BsrI        BssHII      BssSI       Bst1107I    
BstBI       BstEII      BstPI       BstUI       BstXI       
BstZ17I     Bsu36I      Cfr10I      ClaI        CpoI        
DraI        DraII       DraIII      DrdI        DsaI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRI       
EcoRV       EcoT14I     EcoT22I     FseI        FspI        
HgaI        Hin1I       HincII      HindII      HindIII     
HpaI        KasI        KpnI        KspI        MaeI        
MaeII       MamI        MluI        MroI        MslI        
MvnI        NaeI        NarI        NcoI        NdeI        
NgoMIV      NheI        NlaIII      NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacI        SacII       
SalI        SbfI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SnaBI       SpeI        SphI        
Sse8387I    SspI        StuI        StyI        SwaI        
Tth111I     Van91I      XbaI        XcmI        XhoI        
XmnI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

ApaI          119  GGGCCC               GGGCC^C
AvaI          122  CCCGGG               C^[CT]CG[GA]G
BalI          209  TGGCCA               TGG^CCA
BamHI         164  GGATCC               G^GATCC
BanII         119  GGGCCC               G[GA]GC[CT]^C
BclI          401  TGATCA               T^GATCA
BsaI          598  GAGACC               GGTCTC(1/5)
BsaJI         122  CCCGGG               C^C[ACGT][ACGT]GG
BsoBI         122  CCCGGG               C^[CT]CG[GA]G
Bsp1407I      697  TGTACA               T^GTACA
BsrDI         351  CATTGC               GCAATG(2/0)
BsrGI         697  TGTACA               T^GTACA
BstAPI         61  GCAGATGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I        707  GGATG                GGATG(2/0)
EaeI          209  TGGCCA               [CT]^GGCC[GA]
FauI          117  GCGGG                CCCGC(4/6)
FbaI          401  TGATCA               T^GATCA
FokI          707  GGATG                GGATG(9/13)
MaeIII        490  GTCAC                ^GT[ACGT]AC
MfeI          647  CAATTG               C^AATTG
MluNI         209  TGGCCA               TGG^CCA
MscI          209  TGGCCA               TGG^CCA
MseI          295  TTAA                 T^TAA
MspA1I        169  CAGCTG               C[AC]G^C[GT]G
MunI          647  CAATTG               C^AATTG
PleI          451  GAGTC                GAGTC(4/5)
PstI           49  CTGCAG               CTGCA^G
PvuII         169  CAGCTG               CAG^CTG
SapI          181  GAAGAGC              GCTCTTC(1/4)
ScaI          530  AGTACT               AGT^ACT
SfcI           49  CTGCAG               C^T[GA][CT]AG
SmaI          122  CCCGGG               CCC^GGG
SspBI         697  TGTACA               T^GTACA
TaqI           20  TCGA                 T^CGA
TfiI          570  GATTC                G^A[AT]TC
Tru9I         295  TTAA                 T^TAA
Tsp45I        490  GTCAC                ^GT[GC]AC
Tsp509I       648  AATT                 ^AATT
TspRI         213  CACTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I        20  TCGA                 T^CGA
XmaCI         122  CCCGGG               C^CCGGG
XmaI          122  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AfaI          531  GTAC                 GT^AC
              698  GTAC                
AlwNI          43  CAGGTTCTG            CAG[ACGT][ACGT][ACGT]^CTG
              718  CAGACCCTG           
AspHI         194  GTGCTC               G[AT]GC[AT]^C
              688  GAGCAC              
AvaII         189  GGACC                G^G[AT]CC
              714  GGACC               
BbvCI         133  GCTGAGG              CCTCAGC(-5/-2)
              139  GCTGAGG             
BsiHKAI       194  GTGCTC               G[AT]GC[AT]^C
              688  GAGCAC              
BsiYI         123  CCGGGAACAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              192  CCGTGCTCAGG         
BslI          123  CCGGGAACAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              192  CCGTGCTCAGG         
BstYI          38  AGATCC               [GA]^GATC[CT]
              164  GGATCC              
HaeII          86  AGCGCC               [GA]GCGC^[CT]
              203  AGCGCC              
HapII         123  CCGG                 C^CGG
              288  CCGG                
HinfI         451  GAGTC                G^A[ACGT]TC
              570  GATTC               
HpaII         123  CCGG                 C^CGG
              288  CCGG                
MflI           38  AGATCC               [GA]^GATC[CT]
              164  GGATCC              
MspI          123  CCGG                 C^CGG
              288  CCGG                
NlaIV         119  GGGCCC               GG[ACGT]^[ACGT]CC
              164  GGATCC              
RsaI          531  GTAC                 GT^AC
              698  GTAC                
SfaNI          67  GATGC                GCATC(5/9)
              708  GATGC               
XhoII          38  AGATCC               [GA]^GATC[CT]
              164  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   41 enzymes) #
#########################################

AciI        AluI        AlwI        BbvI        BcnI        
BlpI        BmyI        BpmI        Bpu1102I    BseRI       
BsmAI       Bsp1286I    BssKI       BstNI       Cac8I       
CelII       CfoI        Cfr13I      DdeI        DpnII       
EarI        EcoRII      Fnu4HI      HaeIII      HhaI        
HinP1I      HphI        ItaI        Ksp632I     MboI        
MboII       MnlI        MvaI        MwoI        NciI        
NdeII       Sau3AI      Sau96I      ScrFI       SmlI        
TseI        
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