Restriction map for pF1KB5272
 (Linear) (Six-base) MAPSORT of: KIBB5272.seq  Check: 5980  from: 1  to: 744



KIBB5272.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:15  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     502     744 
   Size:        502     242
 
AlwNI CAG_nnn'CTG

Cuts at:      0      48     723     744 
   Size:         48     675      21

  Fragments arranged by size: 

                 675      48      21
 
ApaI G_GGCC'C

Cuts at:      0     123     744 
   Size:        123     621
 
AvaI C'yCGr_G

Cuts at:      0     122     744 
   Size:        122     622
 
BamHI G'GATC_C

Cuts at:      0     164     744 
   Size:        164     580
 
BanII G_rGCy'C

Cuts at:      0     123     744 
   Size:        123     621
 
BbvCI CC'TCA_GC

Cuts at:      0     134     140     744 
   Size:        134       6     604

  Fragments arranged by size: 

                 604     134       6
 
BclI T'GATC_A

Cuts at:      0     401     744 
   Size:        401     343
 
BlpI GC'TnA_GC

Cuts at:      0      83     344     440     744 
   Size:         83     261      96     304

  Fragments arranged by size: 

                 304     261      96      83
 
Bme1580I G_kGCm'C

Cuts at:      0     123     744 
   Size:        123     621
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     165     549     744 
   Size:        165     384     195

  Fragments arranged by size: 

                 384     195     165
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      76     304     490     744 
   Size:         76     228     186     254

  Fragments arranged by size: 

                 254     228     186      76
 
Bpu10I CC'TnA_GC

Cuts at:      0     134     140     197     518     744 
   Size:        134       6      57     321     226

  Fragments arranged by size: 

                 321     226     134      57       6
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     487     532     571     744 
   Size:        487      45      39     173

  Fragments arranged by size: 

                 487     173      45      39
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     592     744 
   Size:        592     152
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      88     487     490     700     744 
   Size:         88     399       3     210      44

  Fragments arranged by size: 

                 399     210      88      44       3
 
BseYI C'CCAG_C

Cuts at:      0       4     744 
   Size:          4     740
 
BsiHKAI G_wGCw'C

Cuts at:      0     198     692     744 
   Size:        198     494      52

  Fragments arranged by size: 

                 494     198      52
 
Bsp1286I G_dGCh'C

Cuts at:      0     123     198     692     744 
   Size:        123      75     494      52

  Fragments arranged by size: 

                 494     123      75      52
 
BsrDI GCAATG_nn'

Cuts at:      0     350     744 
   Size:        350     394
 
BsrGI T'GTAC_A

Cuts at:      0     697     744 
   Size:        697      47
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      67     744 
   Size:         67     677
 
BstYI r'GATC_y

Cuts at:      0      38     164     744 
   Size:         38     126     580

  Fragments arranged by size: 

                 580     126      38
 
BtsI GCAGTG_nn'

Cuts at:      0     212     744 
   Size:        212     532
 
EaeI y'GGCC_r

Cuts at:      0     209     744 
   Size:        209     535
 
EarI CTCTTCn'nnn_

Cuts at:      0     176     353     374     744 
   Size:        176     177      21     370

  Fragments arranged by size: 

                 370     177     176      21
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     538     744 
   Size:        538     206
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      76     304     490     502     744 
   Size:         76     228     186      12     242

  Fragments arranged by size: 

                 242     228     186      76      12
 
HaeII r_GCGC'y

Cuts at:      0      90     207     744 
   Size:         90     117     537

  Fragments arranged by size: 

                 537     117      90
 
MfeI C'AATT_G

Cuts at:      0     647     744 
   Size:        647      97
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     354     744 
   Size:        354     390
 
MscI TGG'CCA

Cuts at:      0     211     744 
   Size:        211     533
 
MspA1I CmG'CkG

Cuts at:      0     171     744 
   Size:        171     573
 
PfoI T'CCnGG_A

Cuts at:      0     320     744 
   Size:        320     424
 
PspOMI G'GGCC_C

Cuts at:      0     119     744 
   Size:        119     625
 
PstI C_TGCA'G

Cuts at:      0      53     744 
   Size:         53     691
 
PvuII CAG'CTG

Cuts at:      0     171     744 
   Size:        171     573
 
SapI GCTCTTCn'nnn_

Cuts at:      0     176     744 
   Size:        176     568
 
ScaI AGT'ACT

Cuts at:      0     532     744 
   Size:        532     212
 
SfcI C'TryA_G

Cuts at:      0      49     744 
   Size:         49     695
 
SmaI CCC'GGG

Cuts at:      0     124     744 
   Size:        124     620
 
SmlI C'TyrA_G

Cuts at:      0     502     511     586     744 
   Size:        502       9      75     158

  Fragments arranged by size: 

                 502     158      75       9
 
TatI w'GTAC_w

Cuts at:      0     530     697     744 
   Size:        530     167      47

  Fragments arranged by size: 

                 530     167      47
 
XmaI C'CCGG_G

Cuts at:      0     122     744 
   Size:        122     622
 

 Enzymes that do cut:

     AcuI    AlwNI     ApaI     AvaI    BamHI    BanII    BbvCI     BclI
     BlpI Bme1580I     BplI     BpmI   Bpu10I    BpuEI     BsaI    BseRI
    BseYI  BsiHKAI Bsp1286I    BsrDI    BsrGI   BstAPI    BstYI     BtsI
     EaeI     EarI     EciI  Eco57MI    HaeII     MfeI     MmeI     MscI
   MspA1I     PfoI   PspOMI     PstI    PvuII     SapI     ScaI     SfcI
     SmaI     SmlI     TatI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI     BanI     BbeI     BbsI     BcgI
    BciVI    BfrBI     BglI    BglII    BmgBI     BmrI     BmtI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI     BsgI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrFI   BssHII    BssSI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI
    CspCI     DraI   DraIII     DrdI     EagI  EcoICRI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    Hin4I   HincII
  HindIII     HpaI     KasI     KpnI     MluI     MslI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI
    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI     XmnI     ZraI
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