Restriction map for
pF1KB0067
(Linear) (Six-base) MAPSORT of: KIBB0067.seq Check: 5679 from: 1 to: 7326
KIBB0067.orf
With 182 enzymes: SgfI *
May 11, 2009 09:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 4916 5125 6243 7326
Size: 4916 209 1118 1083
Fragments arranged by size:
4916 1118 1083 209
AatII G_ACGT'C
Cuts at: 0 3470 7326
Size: 3470 3856
AccI GT'mk_AC
Cuts at: 0 4949 5111 7326
Size: 4949 162 2215
Fragments arranged by size:
4949 2215 162
AclI AA'CG_TT
Cuts at: 0 1169 7326
Size: 1169 6157
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 368 935 3179 3893 4076 4601 6241 7326
Size: 368 567 2244 714 183 525 1640 1085
Fragments arranged by size:
2244 1640 1085 714 567 525 368 183
AfeI AGC'GCT
Cuts at: 0 5320 6195 7326
Size: 5320 875 1131
Fragments arranged by size:
5320 1131 875
AflIII A'CryG_T
Cuts at: 0 1782 1916 4835 5342 7326
Size: 1782 134 2919 507 1984
Fragments arranged by size:
2919 1984 1782 507 134
AgeI A'CCGG_T
Cuts at: 0 1756 7326
Size: 1756 5570
AhdI GACnn_n'nnGTC
Cuts at: 0 1190 3318 7162 7326
Size: 1190 2128 3844 164
Fragments arranged by size:
3844 2128 1190 164
AleI CACnn'nnGTG
Cuts at: 0 874 961 5794 7326
Size: 874 87 4833 1532
Fragments arranged by size:
4833 1532 874 87
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 298 1412 5732 6668 7326
Size: 298 1114 4320 936 658
Fragments arranged by size:
4320 1114 936 658 298
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1678 1710 7326
Size: 1678 32 5616
Fragments arranged by size:
5616 1678 32
AlwNI CAG_nnn'CTG
Cuts at: 0 187 294 1042 1071 1797 2379 2968 4860
Size: 187 107 748 29 726 582 589 1892
Cuts at: 4860 5334 5643 5913 6258 6423 6591 7042 7326
Size: 474 309 270 345 165 168 451 284
Fragments arranged by size:
1892 748 726 589 582 474 451 345
309 284 270 187 168 165 107 29
ApaI G_GGCC'C
Cuts at: 0 428 6032 6152 7326
Size: 428 5604 120 1174
Fragments arranged by size:
5604 1174 428 120
ApaLI G'TGCA_C
Cuts at: 0 2330 2854 5338 7326
Size: 2330 524 2484 1988
Fragments arranged by size:
2484 2330 1988 524
ApoI r'AATT_y
Cuts at: 0 1355 2163 3362 3967 4030 7326
Size: 1355 808 1199 605 63 3296
Fragments arranged by size:
3296 1355 1199 808 605 63
AseI AT'TA_AT
Cuts at: 0 593 7326
Size: 593 6733
AvaI C'yCGr_G
Cuts at: 0 702 2061 3515 4117 5668 5970 6052 7326
Size: 702 1359 1454 602 1551 302 82 1274
Fragments arranged by size:
1551 1454 1359 1274 702 602 302 82
BanI G'GyrC_C
Cuts at: 0 697 2358 2469 4040 5823 6069 7326
Size: 697 1661 111 1571 1783 246 1257
Fragments arranged by size:
1783 1661 1571 1257 697 246 111
BanII G_rGCy'C
Cuts at: 0 364 428 2070 2272 3023 3050 3449 5290
Size: 364 64 1642 202 751 27 399 1841
Cuts at: 5290 6032 6152 6946 7326
Size: 742 120 794 380
Fragments arranged by size:
1841 1642 794 751 742 399 380 364
202 120 64 27
BbeI G_GCGC'C
Cuts at: 0 701 2362 4044 6073 7326
Size: 701 1661 1682 2029 1253
Fragments arranged by size:
2029 1682 1661 1253 701
BbsI GAAGACnn'nnnn_
Cuts at: 0 2672 2998 4022 4589 4884 7326
Size: 2672 326 1024 567 295 2442
Fragments arranged by size:
2672 2442 1024 567 326 295
BbvCI CC'TCA_GC
Cuts at: 0 1720 2309 5633 5658 6239 7326
Size: 1720 589 3324 25 581 1087
Fragments arranged by size:
3324 1720 1087 589 581 25
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 693 727 2835 2869 7198 7232 7326
Size: 693 34 2108 34 4329 34 94
Fragments arranged by size:
4329 2108 693 94 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 7091 7326
Size: 7091 235
BclI T'GATC_A
Cuts at: 0 4467 7326
Size: 4467 2859
BfrBI ATG'CAT
Cuts at: 0 3870 7326
Size: 3870 3456
BglI GCCn_nnn'nGGC
Cuts at: 0 299 2065 4491 6054 6136 6436 6661 7326
Size: 299 1766 2426 1563 82 300 225 665
Fragments arranged by size:
2426 1766 1563 665 300 299 225 82
BlpI GC'TnA_GC
Cuts at: 0 2069 4966 5308 7326
Size: 2069 2897 342 2018
Fragments arranged by size:
2897 2069 2018 342
Bme1580I G_kGCm'C
Cuts at: 0 428 2334 2720 2858 4409 5342 5522 6008
Size: 428 1906 386 138 1551 933 180 486
Cuts at: 6008 6032 6152 6683 7326
Size: 24 120 531 643
Fragments arranged by size:
1906 1551 933 643 531 486 428 386
180 138 120 24
BmgBI CAC'GTC
Cuts at: 0 503 4239 7010 7125 7326
Size: 503 3736 2771 115 201
Fragments arranged by size:
3736 2771 503 201 115
BmrI ACTGGGnnnn_n'
Cuts at: 0 818 879 2714 2791 4009 5034 7056 7326
Size: 818 61 1835 77 1218 1025 2022 270
Fragments arranged by size:
2022 1835 1218 1025 818 270 77 61
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 64 5052 7326
Size: 64 4988 2274
Fragments arranged by size:
4988 2274 64
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 668 869 893 1328 2522 2590 6346 6751
Size: 668 201 24 435 1194 68 3756 405
Cuts at: 6751 7019 7326
Size: 268 307
Fragments arranged by size:
3756 1194 668 435 405 307 268 201
68 24
Bpu10I CC'TnA_GC
Cuts at: 0 1720 2309 5633 5658 6239 7326
Size: 1720 589 3324 25 581 1087
Fragments arranged by size:
3324 1720 1087 589 581 25
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 293 2138 6686 7326
Size: 293 1845 4548 640
Fragments arranged by size:
4548 1845 640 293
BsaI GGTCTCn'nnnn_
Cuts at: 0 715 1346 2097 2992 7037 7326
Size: 715 631 751 895 4045 289
Fragments arranged by size:
4045 895 751 715 631 289
BsaAI yAC'GTr
Cuts at: 0 3459 5139 6324 7326
Size: 3459 1680 1185 1002
Fragments arranged by size:
3459 1680 1185 1002
BsaHI Gr'CG_yC
Cuts at: 0 698 2359 2848 3467 4041 4994 5704 6070
Size: 698 1661 489 619 574 953 710 366
Cuts at: 6070 7326
Size: 1256
Fragments arranged by size:
1661 1256 953 710 698 619 574 489
366
BsaWI w'CCGG_w
Cuts at: 0 1756 7326
Size: 1756 5570
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1420 1450 1678 1708 1725 1755 1998 2028
Size: 1420 30 228 30 17 30 243 30
Cuts at: 2028 2556 2586 2688 2718 3037 3067 4316 4346
Size: 528 30 102 30 319 30 1249 30
Cuts at: 4346 5531 5561 7326
Size: 1185 30 1765
Fragments arranged by size:
1765 1420 1249 1185 528 319 243 228
102 30 30 30 30 30 30 30
30 30 17
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 158 239 553 1733 2624 2916 3082 3277
Size: 158 81 314 1180 891 292 166 195
Cuts at: 3277 3346 4696 5025 7326
Size: 69 1350 329 2301
Fragments arranged by size:
2301 1350 1180 891 329 314 292 195
166 158 81 69
BseYI C'CCAG_C
Cuts at: 0 391 1198 1432 2505 2639 2861 2954 3350
Size: 391 807 234 1073 134 222 93 396
Cuts at: 3350 4822 4915 5238 5585 5689 5720 5764 5846
Size: 1472 93 323 347 104 31 44 82
Cuts at: 5846 5897 6040 6080 6145 6160 6190 6229 6350
Size: 51 143 40 65 15 30 39 121
Cuts at: 6350 6368 6386 6409 6664 6834 6947 7067 7106
Size: 18 18 23 255 170 113 120 39
Cuts at: 7106 7229 7243 7326
Size: 123 14 83
Fragments arranged by size:
1472 1073 807 396 391 347 323 255
234 222 170 143 134 123 121 120
113 104 93 93 83 82 65 51
44 40 39 39 31 30 23 18
18 15 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 304 1248 2246 2542 3871 4894 5068 5142
Size: 304 944 998 296 1329 1023 174 74
Cuts at: 5142 5425 6187 7326
Size: 283 762 1139
Fragments arranged by size:
1329 1139 1023 998 944 762 304 296
283 174 74
BsiEI CG_ry'CG
Cuts at: 0 6140 7326
Size: 6140 1186
BsiHKAI G_wGCw'C
Cuts at: 0 2334 2743 2858 4705 5342 7326
Size: 2334 409 115 1847 637 1984
Fragments arranged by size:
2334 1984 1847 637 409 115
BsmI GAATG_Cn'
Cuts at: 0 537 674 1401 2309 4238 4755 6432 7326
Size: 537 137 727 908 1929 517 1677 894
Fragments arranged by size:
1929 1677 908 894 727 537 517 137
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1098 5137 7131 7326
Size: 1098 4039 1994 195
Fragments arranged by size:
4039 1994 1098 195
Bsp1286I G_dGCh'C
Cuts at: 0 364 428 2070 2272 2334 2720 2743 2858
Size: 364 64 1642 202 62 386 23 115
Cuts at: 2858 3023 3050 3449 4409 4705 5290 5342 5522
Size: 165 27 399 960 296 585 52 180
Cuts at: 5522 6008 6032 6152 6683 6946 7326
Size: 486 24 120 531 263 380
Fragments arranged by size:
1642 960 585 531 486 399 386 380
364 296 263 202 180 165 120 115
64 62 52 27 24 23
BspHI T'CATG_A
Cuts at: 0 623 6518 7326
Size: 623 5895 808
Fragments arranged by size:
5895 808 623
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 723 1805 4916 5125 6215 6243 7326
Size: 723 1082 3111 209 1090 28 1083
Fragments arranged by size:
3111 1090 1083 1082 723 209 28
BsrBI CCG'CTC
Cuts at: 0 2365 4539 5048 6026 7326
Size: 2365 2174 509 978 1300
Fragments arranged by size:
2365 2174 1300 978 509
BsrDI GCAATG_nn'
Cuts at: 0 4452 7226 7326
Size: 4452 2774 100
Fragments arranged by size:
4452 2774 100
BsrFI r'CCGG_y
Cuts at: 0 1756 5303 5597 5826 6655 6723 7326
Size: 1756 3547 294 229 829 68 603
Fragments arranged by size:
3547 1756 829 603 294 229 68
BsrGI T'GTAC_A
Cuts at: 0 4286 7326
Size: 4286 3040
BssSI C'ACGA_G
Cuts at: 0 2222 4275 7326
Size: 2222 2053 3051
Fragments arranged by size:
3051 2222 2053
BstAPI GCAn_nnn'nTGC
Cuts at: 0 5126 5165 5192 5574 5643 6093 6207 6591
Size: 5126 39 27 382 69 450 114 384
Cuts at: 6591 6747 6870 6957 7326
Size: 156 123 87 369
Fragments arranged by size:
5126 450 384 382 369 156 123 114
87 69 39 27
BstEII G'GTnAC_C
Cuts at: 0 2775 7326
Size: 2775 4551
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2184 2869 3999 6324 7326
Size: 2184 685 1130 2325 1002
Fragments arranged by size:
2325 2184 1130 1002 685
BstYI r'GATC_y
Cuts at: 0 631 1038 3345 3728 4355 5561 7326
Size: 631 407 2307 383 627 1206 1765
Fragments arranged by size:
2307 1765 1206 631 627 407 383
Bsu36I CC'TnA_GG
Cuts at: 0 1505 2513 7326
Size: 1505 1008 4813
Fragments arranged by size:
4813 1505 1008
BtgI C'CryG_G
Cuts at: 0 350 2213 2582 3395 4871 5792 6140 6448
Size: 350 1863 369 813 1476 921 348 308
Cuts at: 6448 6779 7054 7093 7169 7289 7326
Size: 331 275 39 76 120 37
Fragments arranged by size:
1863 1476 921 813 369 350 348 331
308 275 120 76 39 37
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2428 2816 3683 5451 6493 7326
Size: 2428 388 867 1768 1042 833
Fragments arranged by size:
2428 1768 1042 867 833 388
BtsI GCAGTG_nn'
Cuts at: 0 1031 2554 4703 5154 5493 6064 7326
Size: 1031 1523 2149 451 339 571 1262
Fragments arranged by size:
2149 1523 1262 1031 571 451 339
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 3122 3157 3611 3646 3837 3872 7326
Size: 3122 35 454 35 191 35 3454
Fragments arranged by size:
3454 3122 454 191 35 35 35
DraI TTT'AAA
Cuts at: 0 161 3255 7326
Size: 161 3094 4071
Fragments arranged by size:
4071 3094 161
DraIII CAC_nnn'GTG
Cuts at: 0 5139 5165 7326
Size: 5139 26 2161
Fragments arranged by size:
5139 2161 26
DrdI GACnn_nn'nnGTC
Cuts at: 0 1277 5107 6222 7326
Size: 1277 3830 1115 1104
Fragments arranged by size:
3830 1277 1115 1104
EaeI y'GGCC_r
Cuts at: 0 300 335 579 791 1515 2285 2933 3858
Size: 300 35 244 212 724 770 648 925
Cuts at: 3858 4085 4321 5606 6137 6326 6684 6725 6813
Size: 227 236 1285 531 189 358 41 88
Cuts at: 6813 6993 7172 7326
Size: 180 179 154
Fragments arranged by size:
1285 925 770 724 648 531 358 300
244 236 227 212 189 180 179 154
88 41 35
EagI C'GGCC_G
Cuts at: 0 6137 7326
Size: 6137 1189
EarI CTCTTCn'nnn_
Cuts at: 0 1655 2489 3182 4643 4673 4691 5200 7326
Size: 1655 834 693 1461 30 18 509 2126
Fragments arranged by size:
2126 1655 1461 834 693 509 30 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 5539 5616 7326
Size: 5539 77 1710
Fragments arranged by size:
5539 1710 77
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 368 668 869 893 935 1328 2522 2590
Size: 368 300 201 24 42 393 1194 68
Cuts at: 2590 3179 3893 4076 4601 6241 6346 6751 7019
Size: 589 714 183 525 1640 105 405 268
Cuts at: 7019 7326
Size: 307
Fragments arranged by size:
1640 1194 714 589 525 405 393 368
307 300 268 201 183 105 68 42
24
EcoNI CCTnn'n_nnAGG
Cuts at: 0 730 5578 7326
Size: 730 4848 1748
Fragments arranged by size:
4848 1748 730
EcoO109I rG'GnC_Cy
Cuts at: 0 291 1027 1794 2218 2509 2965 3276 3312
Size: 291 736 767 424 291 456 311 36
Cuts at: 3312 5672 5966 6149 6706 6917 7326
Size: 2360 294 183 557 211 409
Fragments arranged by size:
2360 767 736 557 456 424 409 311
294 291 291 211 183 36
EcoRI G'AATT_C
Cuts at: 0 1355 2163 3362 7326
Size: 1355 808 1199 3964
Fragments arranged by size:
3964 1355 1199 808
EcoRV GAT'ATC
Cuts at: 0 939 6119 7326
Size: 939 5180 1207
Fragments arranged by size:
5180 1207 939
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 4908 7326
Size: 4908 2418
HaeII r_GCGC'y
Cuts at: 0 272 701 2362 4044 5322 6073 6197 7326
Size: 272 429 1661 1682 1278 751 124 1129
Fragments arranged by size:
1682 1661 1278 1129 751 429 272 124
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 76 108 506 538 1176 1208 1229 1261
Size: 76 32 398 32 638 32 21 32
Cuts at: 1261 1420 1452 1542 1574 2619 2651 3037 3069
Size: 159 32 90 32 1045 32 386 32
Cuts at: 3069 3109 3141 3304 3336 5263 5295 6540 6572
Size: 40 32 163 32 1927 32 1245 32
Cuts at: 6572 6780 6812 7148 7180 7326
Size: 208 32 336 32 146
Fragments arranged by size:
1927 1245 1045 638 398 386 336 208
163 159 146 90 76 40 32 32
32 32 32 32 32 32 32 32
32 32 32 32 21
HincII GTy'rAC
Cuts at: 0 912 3221 4950 7326
Size: 912 2309 1729 2376
Fragments arranged by size:
2376 2309 1729 912
HindIII A'AGCT_T
Cuts at: 0 3538 7326
Size: 3538 3788
KasI G'GCGC_C
Cuts at: 0 697 2358 4040 6069 7326
Size: 697 1661 1682 2029 1257
Fragments arranged by size:
2029 1682 1661 1257 697
MfeI C'AATT_G
Cuts at: 0 1016 1367 7326
Size: 1016 351 5959
Fragments arranged by size:
5959 1016 351
MluI A'CGCG_T
Cuts at: 0 5342 7326
Size: 5342 1984
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 191 1678 1721 7326
Size: 191 1487 43 5605
Fragments arranged by size:
5605 1487 191 43
MscI TGG'CCA
Cuts at: 0 302 337 581 793 1517 2287 2935 4087
Size: 302 35 244 212 724 770 648 1152
Cuts at: 4087 5608 6328 6815 6995 7174 7326
Size: 1521 720 487 180 179 152
Fragments arranged by size:
1521 1152 770 724 720 648 487 302
244 212 180 179 152 35
MslI CAynn'nnrTG
Cuts at: 0 874 961 1781 3707 5074 5794 5959 7326
Size: 874 87 820 1926 1367 720 165 1367
Fragments arranged by size:
1926 1367 1367 874 820 720 165 87
MspA1I CmG'CkG
Cuts at: 0 233 436 1071 1497 2408 3651 4970 5755
Size: 233 203 635 426 911 1243 1319 785
Cuts at: 5755 5863 6450 6588 6879 7233 7326
Size: 108 587 138 291 354 93
Fragments arranged by size:
1319 1243 911 785 635 587 426 354
291 233 203 138 108 93
NaeI GCC'GGC
Cuts at: 0 5305 5599 5828 6657 7326
Size: 5305 294 229 829 669
Fragments arranged by size:
5305 829 669 294 229
NarI GG'CG_CC
Cuts at: 0 698 2359 4041 6070 7326
Size: 698 1661 1682 2029 1256
Fragments arranged by size:
2029 1682 1661 1256 698
NcoI C'CATG_G
Cuts at: 0 350 2213 3395 4871 6779 7169 7326
Size: 350 1863 1182 1476 1908 390 157
Fragments arranged by size:
1908 1863 1476 1182 390 350 157
NgoMIV G'CCGG_C
Cuts at: 0 5303 5597 5826 6655 7326
Size: 5303 294 229 829 671
Fragments arranged by size:
5303 829 671 294 229
NruI TCG'CGA
Cuts at: 0 3668 7326
Size: 3668 3658
NsiI A_TGCA'T
Cuts at: 0 3872 7326
Size: 3872 3454
NspI r_CATG'y
Cuts at: 0 1786 1920 2145 2505 2598 4830 5073 5748
Size: 1786 134 225 360 93 2232 243 675
Cuts at: 5748 5943 6048 6345 6381 6399 6750 6960 7326
Size: 195 105 297 36 18 351 210 366
Fragments arranged by size:
2232 1786 675 366 360 351 297 243
225 210 195 134 105 93 36 18
PacI TTA_AT'TAA
Cuts at: 0 593 7326
Size: 593 6733
PasI CC'CwG_GG
Cuts at: 0 1569 1662 3750 5091 6498 7326
Size: 1569 93 2088 1341 1407 828
Fragments arranged by size:
2088 1569 1407 1341 828 93
PciI A'CATG_T
Cuts at: 0 1782 1916 7326
Size: 1782 134 5410
Fragments arranged by size:
5410 1782 134
PflMI CCAn_nnn'nTGG
Cuts at: 0 1351 5223 5334 7326
Size: 1351 3872 111 1992
Fragments arranged by size:
3872 1992 1351 111
PmlI CAC'GTG
Cuts at: 0 5139 7326
Size: 5139 2187
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1678 1710 4167 4199 7326
Size: 1678 32 2457 32 3127
Fragments arranged by size:
3127 2457 1678 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 1794 2218 2965 3312 5672 5966 6706 6917
Size: 1794 424 747 347 2360 294 740 211
Cuts at: 6917 7326
Size: 409
Fragments arranged by size:
2360 1794 747 740 424 409 347 294
211
PshAI GACnn'nnGTC
Cuts at: 0 2121 7326
Size: 2121 5205
PspOMI G'GGCC_C
Cuts at: 0 424 6028 6148 7326
Size: 424 5604 120 1178
Fragments arranged by size:
5604 1178 424 120
PspXI vC'TCGA_Gb
Cuts at: 0 702 7326
Size: 702 6624
PstI C_TGCA'G
Cuts at: 0 1038 1502 3989 4499 4710 5579 5862 6419
Size: 1038 464 2487 510 211 869 283 557
Cuts at: 6419 6596 6875 6890 7326
Size: 177 279 15 436
Fragments arranged by size:
2487 1038 869 557 510 464 436 283
279 211 177 15
PvuII CAG'CTG
Cuts at: 0 1071 1497 3651 4970 5755 6588 7233 7326
Size: 1071 426 2154 1319 785 833 645 93
Fragments arranged by size:
2154 1319 1071 833 785 645 426 93
RsrII CG'GwC_CG
Cuts at: 0 22 7326
Size: 22 7304
SacII CC_GC'GG
Cuts at: 0 6451 7326
Size: 6451 875
SalI G'TCGA_C
Cuts at: 0 4948 7326
Size: 4948 2378
SanDI GG'GwC_CC
Cuts at: 0 5672 5966 6917 7326
Size: 5672 294 951 409
Fragments arranged by size:
5672 951 409 294
SapI GCTCTTCn'nnn_
Cuts at: 0 1655 2489 5200 7326
Size: 1655 834 2711 2126
Fragments arranged by size:
2711 2126 1655 834
SexAI A'CCwGG_T
Cuts at: 0 516 1883 6017 7022 7326
Size: 516 1367 4134 1005 304
Fragments arranged by size:
4134 1367 1005 516 304
SfcI C'TryA_G
Cuts at: 0 932 1034 1498 2747 3341 3681 3985 4495
Size: 932 102 464 1249 594 340 304 510
Cuts at: 4495 4706 5575 5858 6415 6592 6871 6886 7326
Size: 211 869 283 557 177 279 15 440
Fragments arranged by size:
1249 932 869 594 557 510 464 440
340 304 283 279 211 177 102 15
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 299 6136 7326
Size: 299 5837 1190
Fragments arranged by size:
5837 1190 299
SfoI GGC'GCC
Cuts at: 0 699 2360 4042 6071 7326
Size: 699 1661 1682 2029 1255
Fragments arranged by size:
2029 1682 1661 1255 699
SgrAI Cr'CCGG_yG
Cuts at: 0 1756 5597 7326
Size: 1756 3841 1729
Fragments arranged by size:
3841 1756 1729
SmaI CCC'GGG
Cuts at: 0 2063 4119 5670 5972 6054 7326
Size: 2063 2056 1551 302 82 1272
Fragments arranged by size:
2063 2056 1551 1272 302 82
SmlI C'TyrA_G
Cuts at: 0 308 702 2153 6701 7326
Size: 308 394 1451 4548 625
Fragments arranged by size:
4548 1451 625 394 308
SphI G_CATG'C
Cuts at: 0 2145 2598 4830 5073 5943 6048 6345 6381
Size: 2145 453 2232 243 870 105 297 36
Cuts at: 6381 6399 6750 7326
Size: 18 351 576
Fragments arranged by size:
2232 2145 870 576 453 351 297 243
105 36 18
SspI AAT'ATT
Cuts at: 0 1948 7326
Size: 1948 5378
StuI AGG'CCT
Cuts at: 0 155 560 6111 6488 6508 6979 7326
Size: 155 405 5551 377 20 471 347
Fragments arranged by size:
5551 471 405 377 347 155 20
StyI C'CwwG_G
Cuts at: 0 350 2183 2213 3395 3585 4601 4871 5414
Size: 350 1833 30 1182 190 1016 270 543
Cuts at: 5414 6779 7169 7326
Size: 1365 390 157
Fragments arranged by size:
1833 1365 1182 1016 543 390 350 270
190 157 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 286 569 2226 2317 2576 3742 4034 4475
Size: 286 283 1657 91 259 1166 292 441
Cuts at: 4475 5104 6189 6204 7326
Size: 629 1085 15 1122
Fragments arranged by size:
1657 1166 1122 1085 629 441 292 286
283 259 91 15
TatI w'GTAC_w
Cuts at: 0 4286 7326
Size: 4286 3040
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1023 1055 2688 2720 4316 4348 7326
Size: 1023 32 1633 32 1596 32 2978
Fragments arranged by size:
2978 1633 1596 1023 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2547 3466 3571 7326
Size: 2547 919 105 3755
Fragments arranged by size:
3755 2547 919 105
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2346 4912 6786 7326
Size: 2346 2566 1874 540
Fragments arranged by size:
2566 2346 1874 540
XhoI C'TCGA_G
Cuts at: 0 702 7326
Size: 702 6624
XmaI C'CCGG_G
Cuts at: 0 2061 4117 5668 5970 6052 7326
Size: 2061 2056 1551 302 82 1274
Fragments arranged by size:
2061 2056 1551 1274 302 82
XmnI GAAnn'nnTTC
Cuts at: 0 2392 3718 7326
Size: 2392 1326 3608
Fragments arranged by size:
3608 2392 1326
ZraI GAC'GTC
Cuts at: 0 3468 7326
Size: 3468 3858
Enzymes that do cut:
AarI AatII AccI AclI AcuI AfeI AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AvaI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI HaeII Hin4I
HincII HindIII KasI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NruI NsiI NspI
PacI PasI PciI PflMI PmlI PpiI PpuMI PshAI
PspOMI PspXI PstI PvuII RsrII SacII SalI SanDI
SapI SexAI SfcI SfiI SfoI SgrAI SmaI SmlI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
Acc65I AflII AscI AsiSI AvrII BaeI BamHI BglII
BmtI BsaBI BsiWI BspEI BssHII BstBI BstZ17I ClaI
EcoICRI FseI FspI FspAI HpaI KpnI NdeI NheI
NotI PfoI PmeI PsiI PsrI PvuI SacI SbfI
ScaI SgfI SnaBI SpeI SrfI SwaI XbaI