Restriction map for
pF1KB7354
(Circular) (Six-base) MAPSORT of: pF1KB7354.seq Check: 8077 from: 1 to: 6644
pF1KB7354 6644 bp
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AccI GT'mk_AC
Cuts at: 887 1033 1410 2912 3682 887
Size: 146 377 1502 770 3849
Fragments arranged by size:
3849 1502 770 377 146
Acc65I G'GTAC_C
Cuts at: 3660 3660
Size: 6644
AclI AA'CG_TT
Cuts at: 6484 6484
Size: 6644
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 676 1025 1067 1427 1775 1850 2997 4469 4901
Size: 349 42 360 348 75 1147 1472 432
Cuts at: 4901 5648 5916 676
Size: 747 268 1404
Fragments arranged by size:
1472 1404 1147 747 432 360 349 348
268 75 42
AflII C'TTAA_G
Cuts at: 2973 2973
Size: 6644
AflIII A'CryG_T
Cuts at: 663 1316 3352 3996 5100 6077 663
Size: 653 2036 644 1104 977 1230
Fragments arranged by size:
2036 1230 1104 977 653 644
AgeI A'CCGG_T
Cuts at: 5032 5032
Size: 6644
AlwNI CAG_nnn'CTG
Cuts at: 132 409 1589 3306 3949 5516 132
Size: 277 1180 1717 643 1567 1260
Fragments arranged by size:
1717 1567 1260 1180 643 277
ApaLI G'TGCA_C
Cuts at: 5414 5414
Size: 6644
ApoI r'AATT_y
Cuts at: 220 264 279 563 869 1201 1819 1949 1963
Size: 44 15 284 306 332 618 130 14
Cuts at: 1963 2008 3648 5997 6253 220
Size: 45 1640 2349 256 611
Fragments arranged by size:
2349 1640 618 611 332 306 284 256
130 45 44 15 14
AseI AT'TA_AT
Cuts at: 20 1379 1403 20
Size: 1359 24 5261
Fragments arranged by size:
5261 1359 24
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6644
AvaI C'yCGr_G
Cuts at: 3664 3664
Size: 6644
BamHI G'GATC_C
Cuts at: 119 3040 3576 3669 119
Size: 2921 536 93 3094
Fragments arranged by size:
3094 2921 536 93
BanI G'GyrC_C
Cuts at: 1659 1860 3660 4074 4322 4357 1659
Size: 201 1800 414 248 35 3946
Fragments arranged by size:
3946 1800 414 248 201 35
BanII G_rGCy'C
Cuts at: 474 3274 3658 4688 474
Size: 2800 384 1030 2430
Fragments arranged by size:
2800 2430 1030 384
BbeI G_GCGC'C
Cuts at: 4078 4326 4078
Size: 248 6396
BbsI GAAGACnn'nnnn_
Cuts at: 664 2156 2643 3114 664
Size: 1492 487 471 4194
Fragments arranged by size:
4194 1492 487 471
BciVI GTATCCnnnnn_n'
Cuts at: 4542 5303 6644 4542
Size: 761 1341 4542
Fragments arranged by size:
4542 1341 761
BclI T'GATC_A
Cuts at: 779 4164 779
Size: 3385 3259
BfrBI ATG'CAT
Cuts at: 1684 1684
Size: 6644
BglII A'GATC_T
Cuts at: 4159 6196 4159
Size: 2037 4607
BlpI GC'TnA_GC
Cuts at: 3756 3756
Size: 6644
Bme1580I G_kGCm'C
Cuts at: 4269 4362 5418 4269
Size: 93 1056 5495
Fragments arranged by size:
5495 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 2234 2778 3856 3937 4272 6416 2234
Size: 544 1078 81 335 2144 2462
Fragments arranged by size:
2462 2144 1078 544 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3271 5011 3271
Size: 1740 4904
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1253 3820 4699 5191 5489 1253
Size: 2567 879 492 298 2408
Fragments arranged by size:
2567 2408 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 6348 6348
Size: 6644
BsaAI yAC'GTr
Cuts at: 177 1317 3997 177
Size: 1140 2680 2824
Fragments arranged by size:
2824 2680 1140
BsaBI GATnn'nnATC
Cuts at: 6 1235 1241 1829 4158 6195 6
Size: 1229 6 588 2329 2037 455
Fragments arranged by size:
2329 2037 1229 588 455 6
BsaHI Gr'CG_yC
Cuts at: 4075 4323 5025 6521 4075
Size: 248 702 1496 4198
Fragments arranged by size:
4198 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3833 4354 5032 5306 5453 5864 3833
Size: 521 678 274 147 411 4613
Fragments arranged by size:
4613 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 461 491 1830 1860 461
Size: 30 1339 30 5245
Fragments arranged by size:
5245 1339 30 30
BseYI C'CCAG_C
Cuts at: 132 1226 4069 4827 5404 5900 132
Size: 1094 2843 758 577 496 876
Fragments arranged by size:
2843 1094 876 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1611 1611
Size: 6644
BsiEI CG_ry'CG
Cuts at: 104 4186 4232 5440 104
Size: 4082 46 1208 1308
Fragments arranged by size:
4082 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 474 3658 4436 4626 5418 474
Size: 3184 778 190 792 1700
Fragments arranged by size:
3184 1700 792 778 190
BsmI GAATG_Cn'
Cuts at: 739 3518 739
Size: 2779 3865
Bsp1286I G_dGCh'C
Cuts at: 474 3274 3658 4269 4362 4436 4626 4688 5418
Size: 2800 384 611 93 74 190 62 730
Cuts at: 5418 474
Size: 1700
Fragments arranged by size:
2800 1700 730 611 384 190 93 74
62
BspEI T'CCGG_A
Cuts at: 5864 5864
Size: 6644
BspHI T'CATG_A
Cuts at: 1245 5820 1245
Size: 4575 2069
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3433 3694 3908 4210 4591 3433
Size: 261 214 302 381 5486
Fragments arranged by size:
5486 381 302 261 214
BsrBI CCG'CTC
Cuts at: 50 4936 4990 6474 6639 50
Size: 4886 54 1484 165 55
Fragments arranged by size:
4886 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1617 4556 1617
Size: 2939 3705
BsrFI r'CCGG_y
Cuts at: 4642 4823 5032 4642
Size: 181 209 6254
Fragments arranged by size:
6254 209 181
BsrGI T'GTAC_A
Cuts at: 1599 2742 1599
Size: 1143 5501
BssSI C'ACGA_G
Cuts at: 4915 5273 4915
Size: 358 6286
BstAPI GCAn_nnn'nTGC
Cuts at: 3496 4021 3496
Size: 525 6119
BstBI TT'CG_AA
Cuts at: 489 546 5005 489
Size: 57 4459 2128
Fragments arranged by size:
4459 2128 57
BstXI CCAn_nnnn'nTGG
Cuts at: 3094 3094
Size: 6644
BstYI r'GATC_y
Cuts at: 1 119 1230 3040 3208 3576 3669 4136 4159
Size: 118 1111 1810 168 368 93 467 23
Cuts at: 4159 4494 4740 5752 5838 5850 6196 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1810 1111 1012 467 449 368 346 335
246 168 118 93 86 23 12
BstZ17I GTA'TAC
Cuts at: 888 1034 888
Size: 146 6498
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1649 4594 4775 4938 1649
Size: 2945 181 163 3355
Fragments arranged by size:
3355 2945 181 163
BtsI GCAGTG_nn'
Cuts at: 2497 3887 2497
Size: 1390 5254
DraI TTT'AAA
Cuts at: 1575 3643 1575
Size: 2068 4576
DraIII CAC_nnn'GTG
Cuts at: 260 260
Size: 6644
DrdI GACnn_nn'nnGTC
Cuts at: 4350 5208 4350
Size: 858 5786
EaeI y'GGCC_r
Cuts at: 2268 3084 3259 4229 4403 4794 4821 6166 2268
Size: 816 175 970 174 391 27 1345 2746
Fragments arranged by size:
2746 1345 970 816 391 175 174 27
EagI C'GGCC_G
Cuts at: 4229 4229
Size: 6644
EarI CTCTTCn'nnn_
Cuts at: 294 1289 1446 1573 2075 4667 4877 294
Size: 995 157 127 502 2592 210 2061
Fragments arranged by size:
2592 2061 995 502 210 157 127
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4852 5162 5308 6454 4852
Size: 310 146 1146 5042
Fragments arranged by size:
5042 1146 310 146
EcoICRI GAG'CTC
Cuts at: 472 3656 472
Size: 3184 3460
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 676 1025 1067 1427 1775 1850 2997 4469 4901
Size: 349 42 360 348 75 1147 1472 432
Cuts at: 4901 5648 5916 676
Size: 747 268 1404
Fragments arranged by size:
1472 1404 1147 747 432 360 349 348
268 75 42
EcoO109I rG'GnC_Cy
Cuts at: 3783 3783
Size: 6644
EcoRI G'AATT_C
Cuts at: 1949 3648 1949
Size: 1699 4945
EcoRV GAT'ATC
Cuts at: 3369 3369
Size: 6644
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3399 4663 3399
Size: 1264 5380
FspI TGC'GCA
Cuts at: 4425 4425
Size: 6644
HaeII r_GCGC'y
Cuts at: 117 4078 4326 5348 6327 117
Size: 3961 248 1022 979 434
Fragments arranged by size:
3961 1022 979 434 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 764 796 969 1001 2877 2909 3177 3209 4486
Size: 32 173 32 1876 32 268 32 1277
Cuts at: 4486 4516 4518 4548 764
Size: 30 2 30 2860
Fragments arranged by size:
2860 1876 1277 268 173 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 3683 3683
Size: 6644
KasI G'GCGC_C
Cuts at: 4074 4322 4074
Size: 248 6396
KpnI G_GTAC'C
Cuts at: 3664 3664
Size: 6644
MfeI C'AATT_G
Cuts at: 2338 2338
Size: 6644
MluI A'CGCG_T
Cuts at: 6077 6077
Size: 6644
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1652 1676 3486 3568 5315 5499 1652
Size: 24 1810 82 1747 184 2797
Fragments arranged by size:
2797 1810 1747 184 82 24
MscI TGG'CCA
Cuts at: 2270 3086 3261 4405 2270
Size: 816 175 1144 4509
Fragments arranged by size:
4509 1144 816 175
MslI CAynn'nnrTG
Cuts at: 155 529 895 1958 3130 4633 4760 155
Size: 374 366 1063 1172 1503 127 2039
Fragments arranged by size:
2039 1503 1172 1063 374 366 127
MspA1I CmG'CkG
Cuts at: 132 2076 2095 2245 2473 3755 4069 4429 5442
Size: 1944 19 150 228 1282 314 360 1013
Cuts at: 5442 5687 132
Size: 245 1089
Fragments arranged by size:
1944 1282 1089 1013 360 314 245 228
150 19
NaeI GCC'GGC
Cuts at: 4825 4825
Size: 6644
NarI GG'CG_CC
Cuts at: 4075 4323 4075
Size: 248 6396
NgoMIV G'CCGG_C
Cuts at: 4823 4823
Size: 6644
NsiI A_TGCA'T
Cuts at: 1686 1686
Size: 6644
NspI r_CATG'y
Cuts at: 667 2911 3356 3697 4197 5104 667
Size: 2244 445 341 500 907 2207
Fragments arranged by size:
2244 2207 907 500 445 341
PciI A'CATG_T
Cuts at: 663 3352 5100 663
Size: 2689 1748 2207
Fragments arranged by size:
2689 2207 1748
PflMI CCAn_nnn'nTGG
Cuts at: 795 795
Size: 6644
PfoI T'CCnGG_A
Cuts at: 3476 3476
Size: 6644
PmeI GTTT'AAAC
Cuts at: 3643 3643
Size: 6644
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1550 1582 5783 5815 1550
Size: 32 4201 32 2379
Fragments arranged by size:
4201 2379 32 32
PsiI TTA'TAA
Cuts at: 2305 2759 2305
Size: 454 6190
PstI C_TGCA'G
Cuts at: 576 951 2504 3691 4376 576
Size: 375 1553 1187 685 2844
Fragments arranged by size:
2844 1553 1187 685 375
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6644
PvuII CAG'CTG
Cuts at: 132 2095 2245 2473 4069 4429 132
Size: 1963 150 228 1596 360 2347
Fragments arranged by size:
2347 1963 1596 360 228 150
RsrII CG'GwC_CG
Cuts at: 4839 4839
Size: 6644
SacI G_AGCT'C
Cuts at: 474 3658 474
Size: 3184 3460
SalI G'TCGA_C
Cuts at: 3681 3681
Size: 6644
SapI GCTCTTCn'nnn_
Cuts at: 1289 4667 4877 1289
Size: 3378 210 3056
Fragments arranged by size:
3378 3056 210
SbfI CC_TGCA'GG
Cuts at: 3691 3691
Size: 6644
ScaI AGT'ACT
Cuts at: 1678 3411 6161 1678
Size: 1733 2750 2161
Fragments arranged by size:
2750 2161 1733
SfcI C'TryA_G
Cuts at: 33 572 947 2500 3687 4372 5365 5556 33
Size: 539 375 1553 1187 685 993 191 1121
Fragments arranged by size:
1553 1187 1121 993 685 539 375 191
SfoI GGC'GCC
Cuts at: 4076 4324 4076
Size: 248 6396
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6644
SmaI CCC'GGG
Cuts at: 3666 3666
Size: 6644
SmlI C'TyrA_G
Cuts at: 1268 2973 3799 4714 5206 5468 1268
Size: 1705 826 915 492 262 2444
Fragments arranged by size:
2444 1705 915 826 492 262
SnaBI TAC'GTA
Cuts at: 177 177
Size: 6644
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6644
SphI G_CATG'C
Cuts at: 3697 4197 3697
Size: 500 6144
SspI AAT'ATT
Cuts at: 2859 2859
Size: 6644
StyI C'CwwG_G
Cuts at: 1066 3778 4132 1066
Size: 2712 354 3578
Fragments arranged by size:
3578 2712 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 523 3404 4227 4360 4819 5028 523
Size: 2881 823 133 459 209 2139
Fragments arranged by size:
2881 2139 823 459 209 133
TatI w'GTAC_w
Cuts at: 251 1599 1676 2742 3409 6159 251
Size: 1348 77 1066 667 2750 736
Fragments arranged by size:
2750 1348 1066 736 667 77
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1830 1862 3451 3483 6161 6193 1830
Size: 32 1589 32 2678 32 2281
Fragments arranged by size:
2678 2281 1589 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4441 4441
Size: 6644
XbaI T'CTAG_A
Cuts at: 3675 3675
Size: 6644
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1402 3339 4139 1402
Size: 1937 800 3907
Fragments arranged by size:
3907 1937 800
XmaI C'CCGG_G
Cuts at: 3664 3664
Size: 6644
XmnI GAAnn'nnTTC
Cuts at: 566 2466 566
Size: 1900 4744
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI BstZ17I BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NsiI NspI PciI PflMI PfoI PmeI
PpiI PsiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI SspI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BglI BmgBI BmtI
BpmI Bpu10I BseRI BsiWI BsmBI BssHII BstEII Bsu36I
BtgI ClaI CspCI EcoNI FseI FspAI HindIII HpaI
NcoI NdeI NheI NotI NruI PacI PasI PmlI
PpuMI PshAI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SrfI StuI SwaI XhoI ZraI