Restriction map for pF1KB7334
##################################
# NO CUT ENZYMES (   82 enzymes) #
##################################

AccII       AccIII      AclI        AflII       AgeI        
AhdI        Alw44I      ApaI        ApaLI       AscI        
AspEI       AspI        AviII       BamHI       BbvCI       
BcgI        BfrI        BlpI        Bpu1102I    BsaI        
BseAI       BsgI        BsiEI       BsiWI       BspDI       
BspEI       BspLU11I    BssHII      BssSI       Bst1107I    
BstEII      BstPI       BstUI       BstZ17I     CelII       
ClaI        CpoI        DraI        DraIII      DsaI        
EagI        Eam1105I    EclXI       Eco52I      EcoO65I     
FseI        FspI        HindIII     HpaI        KspI        
MluI        MroI        MvnI        NcoI        NdeI        
NotI        NruI        NspI        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmeI        PshAI       
Psp1406I    PvuI        RsrII       SacII       SalI        
SbfI        SexAI       SfiI        SnaBI       SpeI        
SphI        Sse8387I    StuI        SwaI        Tth111I     
Van91I      XhoI        


###################################
# ONE CUT ENZYMES (   55 enzymes) #
###################################

AatII         169  GACGTC               GACGT^C
Acc65I        491  GGTACC               G^GTACC
AflIII       2548  ACACGT               A^C[GA][CT]GT
AocI         1867  CCTGAGG              CC^T[ACGT]AGG
Aor51HI        92  AGCGCT               AGC^GCT
AseI          421  ATTAAT               AT^TAAT
AsnI          421  ATTAAT               AT^TAAT
Asp718I       491  GGTACC               G^GTACC
AvrII        3979  CCTAGG               C^CTAGG
BalI         1971  TGGCCA               TGG^CCA
BbeI          156  GGCGCC               GGCGC^C
BbrPI        2549  CACGTG               CAC^GTG
BciVI        2743  GGATAC               GTATCC(6/5)
BglI         3462  GCCAATGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI         3979  CCTAGG               C^CTAGG
BsaAI        2549  CACGTG               [CT]AC^GT[GA]
Bsp1407I      524  TGTACA               T^GTACA
BspHI        1305  TCATGA               T^CATGA
BspMI         113  ACCTGC               ACCTGC(4/8)
BsrBI         142  GAGCGG               CCGCTC(-3/-3)
BsrDI        2573  GCAATG               GCAATG(2/0)
BsrFI        3263  GCCGGC               [GA]^CCGG[CT]
BsrGI         524  TGTACA               T^GTACA
BstAPI       3238  GCAGCTGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI        3812  TTCGAA               TT^CGAA
Bsu36I       1867  CCTGAGG              CC^T[ACGT]AGG
Cfr10I       3263  GCCGGC               [GA]^CCGG[CT]
DrdI         1186  GACAGTCCAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III       92  AGCGCT               AGC^GCT
Eco81I       1867  CCTGAGG              CC^T[ACGT]AGG
EcoRI        3322  GAATTC               G^AATTC
EcoT22I       567  ATGCAT               ATGCA^T
KasI          156  GGCGCC               G^GCGCC
KpnI          491  GGTACC               GGTAC^C
MluNI        1971  TGGCCA               TGG^CCA
MscI         1971  TGGCCA               TGG^CCA
NaeI         3263  GCCGGC               GCC^GGC
NarI          156  GGCGCC               GG^CGCC
NgoMIV       3263  GCCGGC               G^CCGGC
NheI         3786  GCTAGC               G^CTAGC
NsiI          567  ATGCAT               ATGCA^T
NspV         3812  TTCGAA               TT^CGAA
PmaCI        2549  CACGTG               CAC^GTG
PmlI         2549  CACGTG               CAC^GTG
PshBI         421  ATTAAT               AT^TAAT
RcaI         1305  TCATGA               T^CATGA
SacI          205  GAGCTC               GAGCT^C
SapI          554  GCTCTTC              GCTCTTC(1/4)
SfoI          156  GGCGCC               GGC^GCC
SfuI         3812  TTCGAA               TT^CGAA
SgrAI        3262  CGCCGGCG             C[GA]^CCGG[CT]G
SmaI           48  CCCGGG               CCC^GGG
SspBI         524  TGTACA               T^GTACA
XmaCI          48  CCCGGG               C^CCGGG
XmaI           48  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AccI         1990  GTAGAC               GT^[AC][GT]AC
             3437  GTAGAC              
AcyI          156  GGCGCC               G[GA]^CG[CT]C
              169  GACGTC              
AlwNI         297  CAGTACCTG            CAG[ACGT][ACGT][ACGT]^CTG
             3239  CAGCTGCTG           
Asp700I      1822  GAAGAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3725  GAAGACTTTC          
AvaI           48  CCCGGG               C^[CT]CG[GA]G
              671  CTCGGG              
AvaII        1201  GGACC                G^G[AT]CC
             3984  GGTCC               
BanI          156  GGCGCC               G^G[CT][GA]CC
              491  GGTACC              
BglII        1644  AGATCT               A^GATCT
             2329  AGATCT              
BsaHI         156  GGCGCC               G[GA]^CG[CT]C
              169  GACGTC              
BsaWI        3902  ACCGGA               [AT]^CCGG[AT]
             4022  ACCGGA              
BsmBI         448  CGTCTC               CGTCTC(1/5)
             3910  GAGACG              
BsmI          349  GAATGC               GAATGC(1/-1)
             1945  GAATGC              
BsoBI          48  CCCGGG               C^[CT]CG[GA]G
              671  CTCGGG              
BstXI         453  CCAGCCAATTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2058  CCAGACCTCTGG        
DraII        1200  GGGACCT              [GA]G^G[ACGT]CC[CT]
             3983  GGGTCCT             
EcoNI        2004  CCTAAAAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             2174  CCTCTGAAAGG         
EcoO109I     1200  GGGACCT              [GA]G^G[ACGT]CC[CT]
             3983  GGGTCCT             
EcoRV        2214  GATATC               GAT^ATC
             3151  GATATC              
EcoT14I      3979  CCTAGG               C^C[AT][AT]GG
             4181  CCAAGG              
HgaI         1795  GACGC                GACGC(5/10)
              447  GCGTC               
Hin1I         156  GGCGCC               G[GA]^CG[CT]C
              169  GACGTC              
HincII       2430  GTCAAC               GT[CT]^[GA]AC
             3033  GTCAAC              
HindII       2430  GTCAAC               GT[CT]^[GA]AC
             3033  GTCAAC              
MslI          560  CATGGAAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             3943  CATGGAGATG          
PpuMI        1200  GGGACCT              [GA]G^G[AT]CC[CT]
             3983  GGGTCCT             
ScaI         1837  AGTACT               AGT^ACT
             1960  AGTACT              
SmlI         1504  CTTGAG               C^T[CT][GA]AG
             3344  CTCAAG              
StyI         3979  CCTAGG               C^C[AT][AT]GG
             4181  CCAAGG              
XmnI         1822  GAAGAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3725  GAAGACTTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   92 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        AspHI       BanII       BbsI        BbvI        
BclI        BcnI        BfaI        BmyI        BpmI        
BpuAI       BsaBI       BsaJI       BseRI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       BstYI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
EaeI        EarI        EcoRII      FauI        FbaI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MamI        MboI        MboII       MfeI        MflI        
MnlI        MseI        MspA1I      MspI        MunI        
MvaI        MwoI        NciI        NdeII       NlaIII      
NlaIV       PleI        PstI        PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SspI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     XbaI        
XcmI        XhoII       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com