Restriction map for pF1KB7302
##################################
# NO CUT ENZYMES (   85 enzymes) #
##################################

Acc65I      AclI        AflII       AgeI        AhdI        
Aor51HI     AscI        AseI        AsnI        Asp718I     
AspEI       AviII       AvrII       BamHI       BbeI        
BbrPI       BcgI        BciVI       BclI        BfrI        
BglII       BlnI        BsiWI       BsmI        BspDI       
BspLU11I    BsrDI       BssHII      BssSI       Bst1107I    
BstBI       BstEII      BstPI       BstZ17I     ClaI        
CpoI        Eam1105I    Eco47III    EcoO65I     EcoRV       
EcoT22I     FbaI        FspI        HincII      HindII      
HpaI        KasI        KpnI        KspI        MfeI        
MluI        MunI        NarI        NcoI        NdeI        
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
RsrII       SacII       SalI        SbfI        ScaI        
SfoI        SfuI        SgrAI       SnaBI       SpeI        
Sse8387I    SspI        SwaI        XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   31 enzymes) #
###################################

AccI         2944  GTCTAC               GT^[AC][GT]AC
AccIII       3227  TCCGGA               T^CCGGA
AflIII       2360  ACGTGT               A^C[GA][CT]GT
Alw44I        414  GTGCAC               G^TGCAC
ApaLI         414  GTGCAC               G^TGCAC
BalI         1644  TGGCCA               TGG^CCA
BsaAI        2359  TACGTG               [CT]AC^GT[GA]
BsaBI        2994  GATGTACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI        3227  TCCGGA               [AT]^CCGG[AT]
BseAI        3227  TCCGGA               T^CCGGA
BsmBI        1478  CGTCTC               CGTCTC(1/5)
BspEI        3227  TCCGGA               T^CCGGA
BspHI         317  TCATGA               T^CATGA
BsrBI        1729  GAGCGG               CCGCTC(-3/-3)
DraI         2298  TTTAAA               TTT^AAA
DrdI         1762  GACCCTGACGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI         1664  CCACGG               C^C[GA][CT]GG
EcoNI         915  CCTAGAGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI        2412  GAATTC               G^AATTC
FseI         3641  GGCCGGCC             GGCCGG^CC
HindIII      3093  AAGCTT               A^AGCTT
MamI         2994  GATGTACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI        1644  TGGCCA               TGG^CCA
MroI         3227  TCCGGA               T^CCGGA
MscI         1644  TGGCCA               TGG^CCA
NaeI         3642  GCCGGC               GCC^GGC
NgoMIV       3642  GCCGGC               G^CCGGC
RcaI          317  TCATGA               T^CATGA
SexAI        2755  ACCTGGT              A^CC[AT]GGT
StuI          457  AGGCCT               AGG^CCT
XcmI         1600  CCAGTGGTTGCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   32 enzymes) #
###################################

AatII        1768  GACGTC               GACGT^C
             3478  GACGTC              
AcsI         2412  GAATTC               [GA]^AATT[CT]
             2712  GAATTT              
ApoI         2412  GAATTC               [GA]^AATT[CT]
             2712  GAATTT              
Asp700I      1716  GAAGCTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2589  GAATTATTTC          
AspI          141  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              907  GACCTTGTC           
BanI          687  GGCACC               G^G[CT][GA]CC
             3310  GGCACC              
BfaI          455  CTAG                 C^TAG
              916  CTAG                
BglI          436  GCCCTTGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1660  GCCTCCACGGC         
BlpI         1514  GCTCAGC              GC^T[ACGT]AGC
             2226  GCTCAGC             
Bpu1102I     1514  GCTCAGC              GC^T[ACGT]AGC
             2226  GCTCAGC             
Bsp1407I     2363  TGTACA               T^GTACA
             2996  TGTACA              
BsrGI        2363  TGTACA               T^GTACA
             2996  TGTACA              
BstAPI        804  GCAGCCAGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             2781  GCAAGGCATGC         
BstXI         758  CCAGGCTGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3131  CCAACGTGGTGG        
CelII        1514  GCTCAGC              GC^T[ACGT]AGC
             2226  GCTCAGC             
DraIII        408  CACAGGGTG            CAC[ACGT][ACGT][ACGT]^GTG
             3661  CACTCGGTG           
EagI         2985  CGGCCG               C^GGCCG
             3644  CGGCCG              
EclXI        2985  CGGCCG               C^GGCCG
             3644  CGGCCG              
Eco52I       2985  CGGCCG               C^GGCCG
             3644  CGGCCG              
MaeI          455  CTAG                 C^TAG
              916  CTAG                
MseI         2299  TTAA                 T^TAA
             2324  TTAA                
PflFI         141  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              907  GACCTTGTC           
PvuII        2581  CAGCTG               CAG^CTG
             2682  CAGCTG              
SfiI          435  GGCCCTTGAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
             1659  GGCCTCCACGGCC       
SmlI          439  CTTGAG               C^T[CT][GA]AG
             1573  CTCAAG              
SphI         2786  GCATGC               GCATG^C
             3245  GCATGC              
SspBI        2363  TGTACA               T^GTACA
             2996  TGTACA              
TaqI         2318  TCGA                 T^CGA
             2849  TCGA                
Tru9I        2299  TTAA                 T^TAA
             2324  TTAA                
Tth111I       141  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              907  GACCTTGTC           
TthHB8I      2318  TCGA                 T^CGA
             2849  TCGA                
XmnI         1716  GAAGCTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2589  GAATTATTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  110 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AlwNI       AocI        ApaI        AspHI       
AvaI        AvaII       BanII       BbsI        BbvCI       
BbvI        BcnI        BmyI        BpmI        BpuAI       
BsaHI       BsaI        BsaJI       BseRI       BsgI        
BsiEI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BspMI       BsrFI       
BsrI        BssKI       BstF5I      BstNI       BstUI       
BstYI       Bsu36I      Cac8I       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       EaeI        
EarI        Eco81I      EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeII       MaeIII      MboI        MboII       MflI        
MnlI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NdeII       NlaIII      
NlaIV       NspI        PflMI       PleI        PpuMI       
PstI        RsaI        SacI        SapI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmaI        
StyI        TfiI        TseI        Tsp45I      Tsp509I     
TspRI       Van91I      XhoII       XmaCI       XmaI        
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com