Restriction map for pF1KB7302
 (Linear) (Six-base) MAPSORT of: KIBB7302.seq  Check: 5561  from: 1  to: 3753



KIBB7302.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:07  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1140    1392    3753 
   Size:       1140     252    2361

  Fragments arranged by size: 

                2361    1140     252
 
AatII G_ACGT'C

Cuts at:      0    1772    3482    3753 
   Size:       1772    1710     271

  Fragments arranged by size: 

                1772    1710     271
 
AccI GT'mk_AC

Cuts at:      0    2945    3753 
   Size:       2945     808
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1184    1271    1307    1586    1868    2218    2944    3753 
   Size:       1184      87      36     279     282     350     726     809

  Fragments arranged by size: 

                1184     809     726     350     282     279      87      36
            
 
AflIII A'CryG_T

Cuts at:      0    2360    3753 
   Size:       2360    1393
 
AlwNI CAG_nnn'CTG

Cuts at:      0     384     495     568    1212    1710    2586    3711    3753 
   Size:        384     111      73     644     498     876    1125      42

  Fragments arranged by size: 

                1125     876     644     498     384     111      73      42
            
 
ApaI G_GGCC'C

Cuts at:      0     261     614    3402    3691    3753 
   Size:        261     353    2788     289      62

  Fragments arranged by size: 

                2788     353     289     261      62
 
ApaLI G'TGCA_C

Cuts at:      0     414    3753 
   Size:        414    3339
 
ApoI r'AATT_y

Cuts at:      0    2412    2712    3753 
   Size:       2412     300    1041

  Fragments arranged by size: 

                2412    1041     300
 
AvaI C'yCGr_G

Cuts at:      0     626    1521    3302    3568    3576    3684    3753 
   Size:        626     895    1781     266       8     108      69

  Fragments arranged by size: 

                1781     895     626     266     108      69       8
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     968    1001    3753 
   Size:        968      33    2752

  Fragments arranged by size: 

                2752     968      33
 
BanI G'GyrC_C

Cuts at:      0     687    3310    3753 
   Size:        687    2623     443

  Fragments arranged by size: 

                2623     687     443
 
BanII G_rGCy'C

Cuts at:      0     261     614     704    1310    1574    1792    3402    3472 
   Size:        261     353      90     606     264     218    1610      70

Cuts at:   3472    3575    3595    3653    3685    3691    3753 
   Size:        103      20      58      32       6      62

  Fragments arranged by size: 

                1610     606     353     264     261     218     103      90
                  70      62      58      32      20       6
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      58    1181    2527    2568    3753 
   Size:         58    1123    1346      41    1185

  Fragments arranged by size: 

                1346    1185    1123      58      41
 
BbvCI CC'TCA_GC

Cuts at:      0      29     512    1346    2347    3753 
   Size:         29     483     834    1001    1406

  Fragments arranged by size: 

                1406    1001     834     483      29
 
BglI GCCn_nnn'nGGC

Cuts at:      0     442    1666    3753 
   Size:        442    1224    2087

  Fragments arranged by size: 

                2087    1224     442
 
BlpI GC'TnA_GC

Cuts at:      0    1515    2227    3753 
   Size:       1515     712    1526

  Fragments arranged by size: 

                1526    1515     712
 
Bme1580I G_kGCm'C

Cuts at:      0     261     418     614    3313    3402    3691    3753 
   Size:        261     157     196    2699      89     289      62

  Fragments arranged by size: 

                2699     289     261     196     157      89      62
 
BmgBI CAC'GTC

Cuts at:      0    2853    3753 
   Size:       2853     900
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     410    1326    1594    2439    2475    3753 
   Size:        410     916     268     845      36    1278

  Fragments arranged by size: 

                1278     916     845     410     268      36
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     654     861    3513    3753 
   Size:        654     207    2652     240

  Fragments arranged by size: 

                2652     654     240     207
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     505     558     572     680     808     908    1687    1814 
   Size:        505      53      14     108     128     100     779     127

Cuts at:   1814    3445    3753 
   Size:       1631     308

  Fragments arranged by size: 

                1631     779     505     308     128     127     108     100
                  53      14
 
Bpu10I CC'TnA_GC

Cuts at:      0      29     512    1346    2347    3753 
   Size:         29     483     834    1001    1406

  Fragments arranged by size: 

                1406    1001     834     483      29
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     460    1558    3753 
   Size:        460    1098    2195

  Fragments arranged by size: 

                2195    1098     460
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     211     625    1318    3587    3753 
   Size:        211     414     693    2269     166

  Fragments arranged by size: 

                2269     693     414     211     166
 
BsaAI yAC'GTr

Cuts at:      0    2361    3753 
   Size:       2361    1392
 
BsaBI GATnn'nnATC

Cuts at:      0    2998    3753 
   Size:       2998     755
 
BsaHI Gr'CG_yC

Cuts at:      0     594    1769    2603    3479    3562    3753 
   Size:        594    1175     834     876      83     191

  Fragments arranged by size: 

                1175     876     834     594     191      83
 
BsaWI w'CCGG_w

Cuts at:      0    3227    3753 
   Size:       3227     526
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     881     911    3753 
   Size:        881      30    2842

  Fragments arranged by size: 

                2842     881      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     145     196     720     934    1069    1084    1087    1102 
   Size:        145      51     524     214     135      15       3      15

Cuts at:   1102    1105    1108    1111    1120    1123    1246    1282    1291 
   Size:          3       3       3       9       3     123      36       9

Cuts at:   1291    1306    1348    1798    1882    2269    3289    3646    3649 
   Size:         15      42     450      84     387    1020     357       3

Cuts at:   3649    3753 
   Size:        104

  Fragments arranged by size: 

                1020     524     450     387     357     214     145     135
                 123     104      84      51      42      36      15      15
                  15       9       9       3       3       3       3       3
                   3
 
BseYI C'CCAG_C

Cuts at:      0       8     292     579     704    1803    2030    3753 
   Size:          8     284     287     125    1099     227    1723

  Fragments arranged by size: 

                1723    1099     287     284     227     125       8
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     763     886    1420    2758    2833    3040    3753 
   Size:        763     123     534    1338      75     207     713

  Fragments arranged by size: 

                1338     763     713     534     207     123      75
 
BsiEI CG_ry'CG

Cuts at:      0    1698    2988    3647    3753 
   Size:       1698    1290     659     106

  Fragments arranged by size: 

                1698    1290     659     106
 
BsiHKAI G_wGCw'C

Cuts at:      0     418    1198    1574    1874    3472    3595    3662    3753 
   Size:        418     780     376     300    1598     123      67      91

  Fragments arranged by size: 

                1598     780     418     376     300     123      91      67
            
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1484    3753 
   Size:       1484    2269
 
Bsp1286I G_dGCh'C

Cuts at:      0     261     418     614     704    1198    1310    1574    1792 
   Size:        261     157     196      90     494     112     264     218

Cuts at:   1792    1874    3313    3402    3472    3575    3595    3653    3662 
   Size:         82    1439      89      70     103      20      58       9

Cuts at:   3662    3685    3691    3753 
   Size:         23       6      62

  Fragments arranged by size: 

                1439     494     264     261     218     196     157     112
                 103      90      89      82      70      62      58      23
                  20       9       6
 
BspEI T'CCGG_A

Cuts at:      0    3227    3753 
   Size:       3227     526
 
BspHI T'CATG_A

Cuts at:      0     317    3753 
   Size:        317    3436
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1140    1392    2231    2957    3012    3753 
   Size:       1140     252     839     726      55     741

  Fragments arranged by size: 

                1140     839     741     726     252      55
 
BsrBI CCG'CTC

Cuts at:      0    1731    3753 
   Size:       1731    2022
 
BsrFI r'CCGG_y

Cuts at:      0    1697    2900    3119    3642    3753 
   Size:       1697    1203     219     523     111

  Fragments arranged by size: 

                1697    1203     523     219     111
 
BsrGI T'GTAC_A

Cuts at:      0    2363    2996    3753 
   Size:       2363     633     757

  Fragments arranged by size: 

                2363     757     633
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     810    2787    3753 
   Size:        810    1977     966

  Fragments arranged by size: 

                1977     966     810
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     765    3138    3753 
   Size:        765    2373     615

  Fragments arranged by size: 

                2373     765     615
 
BstYI r'GATC_y

Cuts at:      0     380    2723    3073    3671    3707    3753 
   Size:        380    2343     350     598      36      46

  Fragments arranged by size: 

                2343     598     380     350      46      36
 
Bsu36I CC'TnA_GG

Cuts at:      0     128     212    1340    3753 
   Size:        128      84    1128    2413

  Fragments arranged by size: 

                2413    1128     128      84
 
BtgI C'CryG_G

Cuts at:      0    1664    3753 
   Size:       1664    2089
 
DraI TTT'AAA

Cuts at:      0    2300    3753 
   Size:       2300    1453
 
DraIII CAC_nnn'GTG

Cuts at:      0     413    3666    3753 
   Size:        413    3253      87

  Fragments arranged by size: 

                3253     413      87
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1768    3753 
   Size:       1768    1985
 
EaeI y'GGCC_r

Cuts at:      0    1644    1667    2985    3128    3416    3644    3753 
   Size:       1644      23    1318     143     288     228     109

  Fragments arranged by size: 

                1644    1318     288     228     143     109      23
 
EagI C'GGCC_G

Cuts at:      0    2985    3644    3753 
   Size:       2985     659     109

  Fragments arranged by size: 

                2985     659     109
 
EarI CTCTTCn'nnn_

Cuts at:      0      65     161     998    1046    1073    1079    1097    1247 
   Size:         65      96     837      48      27       6      18     150

Cuts at:   1247    1283    1439    1562    1726    1859    2920    3166    3274 
   Size:         36     156     123     164     133    1061     246     108

Cuts at:   3274    3328    3650    3753 
   Size:         54     322     103

  Fragments arranged by size: 

                1061     837     322     246     164     156     150     133
                 123     108     103      96      65      54      48      36
                  27      18       6
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     118    1336    2272    3489    3753 
   Size:        118    1218     936    1217     264

  Fragments arranged by size: 

                1218    1217     936     264     118
 
EcoICRI GAG'CTC

Cuts at:      0    1572    3470    3593    3753 
   Size:       1572    1898     123     160

  Fragments arranged by size: 

                1898    1572     160     123
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     505     558     572     680     808     908    1184    1271 
   Size:        505      53      14     108     128     100     276      87

Cuts at:   1271    1307    1586    1687    1814    1868    2218    2944    3445 
   Size:         36     279     101     127      54     350     726     501

Cuts at:   3445    3753 
   Size:        308

  Fragments arranged by size: 

                 726     505     501     350     308     279     276     128
                 127     108     101     100      87      54      53      36
                  14
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     919    3753 
   Size:        919    2834
 
EcoO109I rG'GnC_Cy

Cuts at:      0      41     369     435     610     750     774    1181    2448 
   Size:         41     328      66     175     140      24     407    1267

Cuts at:   2448    3180    3753 
   Size:        732     573

  Fragments arranged by size: 

                1267     732     573     407     328     175     140      66
                  41      24
 
EcoRI G'AATT_C

Cuts at:      0    2412    3753 
   Size:       2412    1341
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    2580    3753 
   Size:       2580    1173
 
FseI GG_CCGG'CC

Cuts at:      0    3646    3753 
   Size:       3646     107
 
HaeII r_GCGC'y

Cuts at:      0     269     586    1685    3753 
   Size:        269     317    1099    2068

  Fragments arranged by size: 

                2068    1099     317     269
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     130     136     162     168    1759    1791    3031    3063 
   Size:        130       6      26       6    1591      32    1240      32

Cuts at:   3063    3692    3724    3753 
   Size:        629      32      29

  Fragments arranged by size: 

                1591    1240     629     130      32      32      32      29
                  26       6       6
 
HindIII A'AGCT_T

Cuts at:      0    3093    3753 
   Size:       3093     660
 
MscI TGG'CCA

Cuts at:      0    1646    3753 
   Size:       1646    2107
 
MslI CAynn'nnrTG

Cuts at:      0     953    2119    2851    3753 
   Size:        953    1166     732     902

  Fragments arranged by size: 

                1166     953     902     732
 
MspA1I CmG'CkG

Cuts at:      0    1846    1950    2294    2583    2684    3753 
   Size:       1846     104     344     289     101    1069

  Fragments arranged by size: 

                1846    1069     344     289     104     101
 
NaeI GCC'GGC

Cuts at:      0    3644    3753 
   Size:       3644     109
 
NgoMIV G'CCGG_C

Cuts at:      0    3642    3753 
   Size:       3642     111
 
NspI r_CATG'y

Cuts at:      0    1887    2184    2790    2922    3249    3753 
   Size:       1887     297     606     132     327     504

  Fragments arranged by size: 

                1887     606     504     327     297     132
 
PasI CC'CwG_GG

Cuts at:      0      37     261     850     935    3753 
   Size:         37     224     589      85    2818

  Fragments arranged by size: 

                2818     589     224      85      37
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     606    1710    2465    3753 
   Size:        606    1104     755    1288

  Fragments arranged by size: 

                1288    1104     755     606
 
PfoI T'CCnGG_A

Cuts at:      0     588     885    1042    1703    2108    3167    3753 
   Size:        588     297     157     661     405    1059     586

  Fragments arranged by size: 

                1059     661     588     586     405     297     157
 
PpuMI rG'GwC_Cy

Cuts at:      0      41     369     750     774    1181    3180    3753 
   Size:         41     328     381      24     407    1999     573

  Fragments arranged by size: 

                1999     573     407     381     328      41      24
 
PspOMI G'GGCC_C

Cuts at:      0     257     610    3398    3687    3753 
   Size:        257     353    2788     289      66

  Fragments arranged by size: 

                2788     353     289     257      66
 
PstI C_TGCA'G

Cuts at:      0     555     623     665    2141    3753 
   Size:        555      68      42    1476    1612

  Fragments arranged by size: 

                1612    1476     555      68      42
 
PvuII CAG'CTG

Cuts at:      0    2583    2684    3753 
   Size:       2583     101    1069

  Fragments arranged by size: 

                2583    1069     101
 
SacI G_AGCT'C

Cuts at:      0    1574    3472    3595    3753 
   Size:       1574    1898     123     158

  Fragments arranged by size: 

                1898    1574     158     123
 
SanDI GG'GwC_CC

Cuts at:      0      41     750    1181    3753 
   Size:         41     709     431    2572

  Fragments arranged by size: 

                2572     709     431      41
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1562    1726    3274    3328    3650    3753 
   Size:       1562     164    1548      54     322     103

  Fragments arranged by size: 

                1562    1548     322     164     103      54
 
SexAI A'CCwGG_T

Cuts at:      0    2755    3753 
   Size:       2755     998
 
SfcI C'TryA_G

Cuts at:      0     551     560     619     661    2137    2397    3753 
   Size:        551       9      59      42    1476     260    1356

  Fragments arranged by size: 

                1476    1356     551     260      59      42       9
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     442    1666    3753 
   Size:        442    1224    2087

  Fragments arranged by size: 

                2087    1224     442
 
SmaI CCC'GGG

Cuts at:      0     628    3304    3578    3686    3753 
   Size:        628    2676     274     108      67

  Fragments arranged by size: 

                2676     628     274     108      67
 
SmlI C'TyrA_G

Cuts at:      0     439    1573    3753 
   Size:        439    1134    2180

  Fragments arranged by size: 

                2180    1134     439
 
SphI G_CATG'C

Cuts at:      0    2790    3249    3753 
   Size:       2790     459     504

  Fragments arranged by size: 

                2790     504     459
 
SrfI GCCC'GGGC

Cuts at:      0    3686    3753 
   Size:       3686      67
 
StuI AGG'CCT

Cuts at:      0     459    3753 
   Size:        459    3294
 
StyI C'CwwG_G

Cuts at:      0     221     668     680    1262    1328    2240    3269    3612 
   Size:        221     447      12     582      66     912    1029     343

Cuts at:   3612    3753 
   Size:        141

  Fragments arranged by size: 

                1029     912     582     447     343     221     141      66
                  12
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     666    1406    3346    3539    3753 
   Size:        666     740    1940     193     214

  Fragments arranged by size: 

                1940     740     666     214     193
 
TatI w'GTAC_w

Cuts at:      0    2303    2363    2705    2765    2996    3753 
   Size:       2303      60     342      60     231     757

  Fragments arranged by size: 

                2303     757     342     231      60      60
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     397     429     881     913    3753 
   Size:        397      32     452      32    2840

  Fragments arranged by size: 

                2840     452     397      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     144     910    3753 
   Size:        144     766    2843

  Fragments arranged by size: 

                2843     766     144
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1607    3753 
   Size:       1607    2146
 
XmaI C'CCGG_G

Cuts at:      0     626    3302    3576    3684    3753 
   Size:        626    2676     274     108      69

  Fragments arranged by size: 

                2676     626     274     108      69
 
XmnI GAAnn'nnTTC

Cuts at:      0    1720    2593    3753 
   Size:       1720     873    1160

  Fragments arranged by size: 

                1720    1160     873
 
ZraI GAC'GTC

Cuts at:      0    1770    3480    3753 
   Size:       1770    1710     273

  Fragments arranged by size: 

                1770    1710     273
 

 Enzymes that do cut:

     AarI    AatII     AccI     AcuI   AflIII    AlwNI     ApaI    ApaLI
     ApoI     AvaI     BaeI     BanI    BanII     BbsI    BbvCI     BglI
     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrFI    BsrGI   BstAPI    BstXI    BstYI   Bsu36I     BtgI
     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FseI    HaeII    Hin4I
  HindIII     MscI     MslI   MspA1I     NaeI   NgoMIV     NspI     PasI
    PflMI     PfoI    PpuMI   PspOMI     PstI    PvuII     SacI    SanDI
     SapI    SexAI     SfcI     SfiI     SmaI     SmlI     SphI     SrfI
     StuI     StyI    TaqII     TatI     TstI  Tth111I     XcmI     XmaI
     XmnI     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI     AfeI    AflII     AgeI     AhdI     AleI     AlfI
     AloI     AscI     AseI    AsiSI    AvrII    BamHI     BbeI     BcgI
    BciVI     BclI    BfrBI    BglII     BmtI    BsiWI     BsmI    BsrDI
   BssHII    BssSI    BstBI   BstEII  BstZ17I    BtgZI     BtsI     ClaI
    CspCI    EcoRV     FspI    FspAI   HincII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     NarI     NcoI     NdeI     NheI     NotI
     NruI     NsiI     PacI     PciI     PmeI     PmlI     PpiI    PshAI
     PsiI    PspXI     PsrI     PvuI    RsrII    SacII     SalI     SbfI
     ScaI     SfoI     SgfI    SgrAI    SnaBI     SpeI     SspI     SwaI
     XbaI     XhoI
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