Restriction map for pF1KB8985
##################################
# NO CUT ENZYMES (  120 enzymes) #
##################################

Acc65I      AccI        AclI        AcsI        AflII       
AgeI        AhdI        AlwNI       AocI        Aor51HI     
ApaI        ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspI        AvrII       
BalI        BamHI       BbrPI       BbsI        BbvCI       
BcgI        BclI        BfrI        BglII       BlnI        
BpuAI       BsaAI       BsaBI       BsiWI       BsmBI       
BsmFI       Bsp1407I    BspDI       BspLU11I    BsrDI       
BsrGI       BssSI       Bst1107I    BstBI       BstZ17I     
Bsu36I      ClaI        CpoI        DraI        DraII       
DraIII      Eam1105I    Eco47III    Eco81I      EcoNI       
EcoO109I    EcoRI       EcoRV       EcoT14I     EcoT22I     
FbaI        FseI        HgaI        HindIII     HpaI        
KpnI        KspI        MamI        MfeI        MluNI       
MscI        MseI        MslI        MspA1I      MunI        
NcoI        NdeI        NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfiI        SfuI        
SgrAI       SnaBI       SpeI        Sse8387I    SspBI       
SspI        StuI        StyI        SwaI        Tru9I       
Tsp509I     Tth111I     XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AatII         369  GACGTC               GACGT^C
AccIII        618  TCCGGA               T^CCGGA
AflIII        824  ACGCGT               A^C[GA][CT]GT
Alw44I       1100  GTGCAC               G^TGCAC
ApaLI        1100  GTGCAC               G^TGCAC
AvaII         645  GGACC                G^G[AT]CC
AviII         887  TGCGCA               TGC^GCA
BbeI         1119  GGCGCC               GGCGC^C
BciVI         420  GTATCC               GTATCC(6/5)
BfaI          794  CTAG                 C^TAG
BlpI          357  GCTCAGC              GC^T[ACGT]AGC
BpmI          682  CTGGAG               CTGGAG(16/14)
Bpu1102I      357  GCTCAGC              GC^T[ACGT]AGC
BsaI          685  GAGACC               GGTCTC(1/5)
BsaWI         618  TCCGGA               [AT]^CCGG[AT]
BseAI         618  TCCGGA               T^CCGGA
BseRI          79  GAGGAG               GAGGAG(10/8)
BsgI          388  GTGCAG               GTGCAG(16/14)
BsmAI         685  GAGAC                GTCTC(1/5)
BsmI          697  GCATTC               GAATGC(1/-1)
BspEI         618  TCCGGA               T^CCGGA
BspHI         314  TCATGA               T^CATGA
BstAPI       1102  GCACCCTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI         599  CCAACGACATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII         357  GCTCAGC              GC^T[ACGT]AGC
DrdI          121  GACTCGGTAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI          833  CGGCCG               C^GGCCG
EclXI         833  CGGCCG               C^GGCCG
Eco52I        833  CGGCCG               C^GGCCG
FspI          887  TGCGCA               TGC^GCA
HincII        658  GTCAAC               GT[CT]^[GA]AC
HindII        658  GTCAAC               GT[CT]^[GA]AC
KasI         1119  GGCGCC               G^GCGCC
MaeI          794  CTAG                 C^TAG
MluI          824  ACGCGT               A^CGCGT
MroI          618  TCCGGA               T^CCGGA
NarI         1119  GGCGCC               GG^CGCC
NspI          929  GCATGC               [GA]CATG^[CT]
PflMI         636  CCAGGGCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI         166  GACATCGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI          314  TCATGA               T^CATGA
SfcI          149  CTACAG               C^T[GA][CT]AG
SfoI         1119  GGCGCC               GGC^GCC
SphI          929  GCATGC               GCATG^C
TaqI          272  TCGA                 T^CGA
TfiI          570  GATTC                G^A[AT]TC
TspRI         916  CAGTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I       272  TCGA                 T^CGA
Van91I        636  CCAGGGCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AcyI          369  GACGTC               G[GA]^CG[CT]C
             1119  GGCGCC              
AfaI           72  GTAC                 GT^AC
              780  GTAC                
AspHI         622  GAGCAC               G[AT]GC[AT]^C
             1100  GTGCAC              
BglI          535  GCCAAGCCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              762  GCCCATCCGGC         
BsaHI         369  GACGTC               G[GA]^CG[CT]C
             1119  GGCGCC              
BsiEI         833  CGGCCG               CG[GA][CT]^CG
              846  CGACCG              
BsiHKAI       622  GAGCAC               G[AT]GC[AT]^C
             1100  GTGCAC              
BspMI         884  ACCTGC               ACCTGC(4/8)
             1153  ACCTGC              
BsrBI         748  CCGCTC               CCGCTC(-3/-3)
              805  CCGCTC              
BsrI          287  ACTGG                ACTGG(1/-1)
              915  CCAGT               
BssHII         92  GCGCGC               G^CGCGC
             1027  GCGCGC              
BstEII         30  GGTGACC              G^GT[ACGT]ACC
             1146  GGTCACC             
BstPI          30  GGTGACC              G^GT[ACGT]ACC
             1146  GGTCACC             
DdeI          358  CTCAG                C^T[ACGT]AG
              462  CTCAG               
DsaI          374  CCACGG               C^C[GA][CT]GG
             1142  CCGTGG              
EarI          103  CTCTTC               CTCTTC(1/4)
              559  CTCTTC              
EcoO65I        30  GGTGACC              G^GT[ACGT]ACC
             1146  GGTCACC             
Hin1I         369  GACGTC               G[GA]^CG[CT]C
             1119  GGCGCC              
Ksp632I       103  CTCTTC               CTCTTC(1/4)
              559  CTCTTC              
MaeII         370  ACGT                 A^CGT
              407  ACGT                
NaeI          322  GCCGGC               GCC^GGC
              540  GCCGGC              
NgoMIV        322  GCCGGC               G^CCGGC
              540  GCCGGC              
PleI          121  GACTC                GAGTC(4/5)
              274  GACTC               
RsaI           72  GTAC                 GT^AC
              780  GTAC                
SmaI          499  CCCGGG               CCC^GGG
              720  CCCGGG              
SmlI          757  CTCAAG               C^T[CT][GA]AG
             1066  CTCAAG              
XmaCI         499  CCCGGG               C^CCGGG
              720  CCCGGG              
XmaI          499  CCCGGG               C^CCGGG
              720  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   61 enzymes) #
#########################################

AccII       AciI        AluI        AlwI        AvaI        
BanI        BanII       BbvI        BcnI        BmyI        
BsaJI       BsiYI       BslI        BsoBI       Bsp1286I    
BsrFI       BssKI       BstF5I      BstNI       BstUI       
BstYI       Cac8I       CfoI        Cfr10I      Cfr13I      
DpnII       EaeI        EcoRII      FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
MaeIII      MboI        MboII       MflI        MnlI        
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       Sau3AI      Sau96I      
ScrFI       SfaNI       TseI        Tsp45I      XcmI        
XhoII       
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