Restriction map for pF1KB9669
 (Linear) (Six-base) MAPSORT of: KIBB9669.seq  Check: 6682  from: 1  to: 1467



KIBB9669.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:08  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     145    1460    1467 
   Size:        145    1315       7

  Fragments arranged by size: 

                1315     145       7
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     253     326     567     735     819     987    1071    1239 
   Size:        253      73     241     168      84     168      84     168

Cuts at:   1239    1407    1467 
   Size:        168      60

  Fragments arranged by size: 

                 253     241     168     168     168     168      84      84
                  73      60
 
AfeI AGC'GCT

Cuts at:      0     508    1036    1467 
   Size:        508     528     431

  Fragments arranged by size: 

                 528     508     431
 
AflIII A'CryG_T

Cuts at:      0     650     725     809     977    1229    1467 
   Size:        650      75      84     168     252     238

  Fragments arranged by size: 

                 650     252     238     168      84      75
 
ApaLI G'TGCA_C

Cuts at:      0     535     607     775    1195    1207    1363    1467 
   Size:        535      72     168     420      12     156     104

  Fragments arranged by size: 

                 535     420     168     156     104      72      12
 
ApoI r'AATT_y

Cuts at:      0     179    1467 
   Size:        179    1288
 
AvaI C'yCGr_G

Cuts at:      0     817    1467 
   Size:        817     650
 
BamHI G'GATC_C

Cuts at:      0      12     449     701    1289    1373    1467 
   Size:         12     437     252     588      84      94

  Fragments arranged by size: 

                 588     437     252      94      84      12
 
BanI G'GyrC_C

Cuts at:      0      85    1467 
   Size:         85    1382
 
BanII G_rGCy'C

Cuts at:      0     211     931    1015    1467 
   Size:        211     720      84     452

  Fragments arranged by size: 

                 720     452     211      84
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      41     679    1423    1467 
   Size:         41     638     744      44

  Fragments arranged by size: 

                 744     638      44      41
 
BglI GCCn_nnn'nGGC

Cuts at:      0     138    1467 
   Size:        138    1329
 
Bme1580I G_kGCm'C

Cuts at:      0     539     611     779     817    1199    1211    1367    1467 
   Size:        539      72     168      38     382      12     156     100

  Fragments arranged by size: 

                 539     382     168     156     100      72      38      12
            
 
BmgBI CAC'GTC

Cuts at:      0     651    1467 
   Size:        651     816
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     318    1467 
   Size:        318    1149
 
BsaAI yAC'GTr

Cuts at:      0     810     978    1230    1467 
   Size:        810     168     252     237

  Fragments arranged by size: 

                 810     252     237     168
 
BsaWI w'CCGG_w

Cuts at:      0     668    1467 
   Size:        668     799
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      28      58     790     820     874     904     913     943 
   Size:         28      30     732      30      54      30       9      30

Cuts at:    943    1467 
   Size:        524

  Fragments arranged by size: 

                 732     524      54      30      30      30      30      28
                   9
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     388     921    1089    1341    1467 
   Size:        388     533     168     252     126

  Fragments arranged by size: 

                 533     388     252     168     126
 
BseYI C'CCAG_C

Cuts at:      0     689    1467 
   Size:        689     778
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     179     928    1086    1467 
   Size:        179     749     158     381

  Fragments arranged by size: 

                 749     381     179     158
 
BsiHKAI G_wGCw'C

Cuts at:      0     539     611     779     863     931    1015    1199    1211 
   Size:        539      72     168      84      68      84     184      12

Cuts at:   1211    1283    1367    1467 
   Size:         72      84     100

  Fragments arranged by size: 

                 539     184     168     100      84      84      84      72
                  72      68      12
 
BsiWI C'GTAC_G

Cuts at:      0     806     890    1467 
   Size:        806      84     577

  Fragments arranged by size: 

                 806     577      84
 
Bsp1286I G_dGCh'C

Cuts at:      0     211     539     611     779     817     863     931    1015 
   Size:        211     328      72     168      38      46      68      84

Cuts at:   1015    1199    1211    1283    1367    1467 
   Size:        184      12      72      84     100

  Fragments arranged by size: 

                 328     211     184     168     100      84      84      72
                  72      68      46      38      12
 
BspEI T'CCGG_A

Cuts at:      0     668    1467 
   Size:        668     799
 
BspHI T'CATG_A

Cuts at:      0     225    1467 
   Size:        225    1242
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     145    1460    1467 
   Size:        145    1315       7

  Fragments arranged by size: 

                1315     145       7
 
BsrBI CCG'CTC

Cuts at:      0     699    1467 
   Size:        699     768
 
BsrFI r'CCGG_y

Cuts at:      0     961    1129    1297    1467 
   Size:        961     168     168     170

  Fragments arranged by size: 

                 961     170     168     168
 
BssSI C'ACGA_G

Cuts at:      0      75     694    1467 
   Size:         75     619     773

  Fragments arranged by size: 

                 773     619      75
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     905    1073    1467 
   Size:        905     168     394

  Fragments arranged by size: 

                 905     394     168
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     128    1467 
   Size:        128    1339
 
BstYI r'GATC_y

Cuts at:      0      12     449     701    1289    1373    1467 
   Size:         12     437     252     588      84      94

  Fragments arranged by size: 

                 588     437     252      94      84      12
 
BtgI C'CryG_G

Cuts at:      0      62     142     271    1467 
   Size:         62      80     129    1196

  Fragments arranged by size: 

                1196     129      80      62
 
EaeI y'GGCC_r

Cuts at:      0     130    1320    1467 
   Size:        130    1190     147

  Fragments arranged by size: 

                1190     147     130
 
EcoICRI GAG'CTC

Cuts at:      0     929    1013    1467 
   Size:        929      84     454

  Fragments arranged by size: 

                 929     454      84
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     253     318     326     567     735     819     987    1071 
   Size:        253      65       8     241     168      84     168      84

Cuts at:   1071    1239    1407    1467 
   Size:        168     168      60

  Fragments arranged by size: 

                 253     241     168     168     168     168      84      84
                  65      60       8
 
EcoO109I rG'GnC_Cy

Cuts at:      0      70      94     299    1467 
   Size:         70      24     205    1168

  Fragments arranged by size: 

                1168     205      70      24
 
EcoRI G'AATT_C

Cuts at:      0     179    1467 
   Size:        179    1288
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1185    1467 
   Size:       1185     282
 
HaeII r_GCGC'y

Cuts at:      0     510    1038    1182    1268    1467 
   Size:        510     528     144      86     199

  Fragments arranged by size: 

                 528     510     199     144      86
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      28      60     496     528     666     698    1467 
   Size:         28      32     436      32     138      32     769

  Fragments arranged by size: 

                 769     436     138      32      32      32      28
 
MluI A'CGCG_T

Cuts at:      0     725    1467 
   Size:        725     742
 
MscI TGG'CCA

Cuts at:      0     132    1467 
   Size:        132    1335
 
MslI CAynn'nnrTG

Cuts at:      0    1048    1467 
   Size:       1048     419
 
MspA1I CmG'CkG

Cuts at:      0     128     144     531    1287    1371    1467 
   Size:        128      16     387     756      84      96

  Fragments arranged by size: 

                 756     387     128      96      84      16
 
NspI r_CATG'y

Cuts at:      0     224     765     849    1467 
   Size:        224     541      84     618

  Fragments arranged by size: 

                 618     541     224      84
 
PasI CC'CwG_GG

Cuts at:      0     186    1467 
   Size:        186    1281
 
PpuMI rG'GwC_Cy

Cuts at:      0      70      94    1467 
   Size:         70      24    1373

  Fragments arranged by size: 

                1373      70      24
 
PstI C_TGCA'G

Cuts at:      0     397    1467 
   Size:        397    1070
 
PvuII CAG'CTG

Cuts at:      0     128    1467 
   Size:        128    1339
 
SacI G_AGCT'C

Cuts at:      0     931    1015    1467 
   Size:        931      84     452

  Fragments arranged by size: 

                 931     452      84
 
SacII CC_GC'GG

Cuts at:      0     145    1467 
   Size:        145    1322
 
SanDI GG'GwC_CC

Cuts at:      0      70    1467 
   Size:         70    1397
 
SfcI C'TryA_G

Cuts at:      0     393    1467 
   Size:        393    1074
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     138    1467 
   Size:        138    1329
 
SgrAI Cr'CCGG_yG

Cuts at:      0     961    1129    1297    1467 
   Size:        961     168     168     170

  Fragments arranged by size: 

                 961     170     168     168
 
StuI AGG'CCT

Cuts at:      0     412     580     664     748     916    1000    1084    1252 
   Size:        412     168      84      84     168      84      84     168

Cuts at:   1252    1467 
   Size:        215

  Fragments arranged by size: 

                 412     215     168     168     168      84      84      84
                  84
 
StyI C'CwwG_G

Cuts at:      0     249    1467 
   Size:        249    1218
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     703     871    1467 
   Size:        703     168     596

  Fragments arranged by size: 

                 703     596     168
 
XmnI GAAnn'nnTTC

Cuts at:      0     496    1057    1168    1336    1420    1467 
   Size:        496     561     111     168      84      47

  Fragments arranged by size: 

                 561     496     168     111      84      47
 

 Enzymes that do cut:

     AarI     AcuI     AfeI   AflIII    ApaLI     ApoI     AvaI    BamHI
     BanI    BanII     BbsI     BglI Bme1580I    BmgBI     BpmI    BsaAI
    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI    BsiWI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrFI    BssSI   BstAPI    BstXI
    BstYI     BtgI     EaeI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI
    HaeII    Hin4I     MluI     MscI     MslI   MspA1I     NspI     PasI
    PpuMI     PstI    PvuII     SacI    SacII    SanDI     SfcI     SfiI
    SgrAI     StuI     StyI    TaqII     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI    AflII     AgeI     AhdI     AleI
     AlfI     AloI    AlwNI     ApaI     AscI     AseI    AsiSI    AvrII
     BaeI     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII
     BlpI     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaBI
    BsaHI    BsiEI     BsmI    BsmBI    BsrDI    BsrGI   BssHII    BstBI
   BstEII  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI     EarI     EciI    EcoNI    EcoRV     FseI
     FspI    FspAI   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MmeI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII
     SalI     SapI     SbfI     ScaI    SexAI     SfoI     SgfI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI
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