Restriction map for pF1KB6259
 (Linear) (Six-base) MAPSORT of: KIBB6259.seq  Check: 4906  from: 1  to: 2292



KIBB6259.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:05  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     892    2292 
   Size:        892    1400
 
AccI GT'mk_AC

Cuts at:      0     293    2292 
   Size:        293    1999
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1970    2292 
   Size:       1970     322
 
AfeI AGC'GCT

Cuts at:      0     841    2292 
   Size:        841    1451
 
AgeI A'CCGG_T

Cuts at:      0    2084    2292 
   Size:       2084     208
 
ApoI r'AATT_y

Cuts at:      0     679     877    1607    1697    2017    2092    2287    2292 
   Size:        679     198     730      90     320      75     195       5

  Fragments arranged by size: 

                 730     679     320     198     195      90      75       5
            
 
AvaI C'yCGr_G

Cuts at:      0    1450    2292 
   Size:       1450     842
 
BanI G'GyrC_C

Cuts at:      0    1249    1296    2271    2292 
   Size:       1249      47     975      21

  Fragments arranged by size: 

                1249     975      47      21
 
BbeI G_GCGC'C

Cuts at:      0    1300    2292 
   Size:       1300     992
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      93     772    2292 
   Size:         93     679    1520

  Fragments arranged by size: 

                1520     679      93
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     825     918    2292 
   Size:        825      93    1374

  Fragments arranged by size: 

                1374     825      93
 
BfrBI ATG'CAT

Cuts at:      0     322    2292 
   Size:        322    1970
 
Bme1580I G_kGCm'C

Cuts at:      0     866    2292 
   Size:        866    1426
 
BmgBI CAC'GTC

Cuts at:      0     996    2292 
   Size:        996    1296
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     298    1147    2192    2292 
   Size:        298     849    1045     100

  Fragments arranged by size: 

                1045     849     298     100
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1176    2292 
   Size:       1176    1116
 
BsaAI yAC'GTr

Cuts at:      0    2254    2292 
   Size:       2254      38
 
BsaBI GATnn'nnATC

Cuts at:      0    1112    1620    2292 
   Size:       1112     508     672

  Fragments arranged by size: 

                1112     672     508
 
BsaHI Gr'CG_yC

Cuts at:      0    1297    2292 
   Size:       1297     995
 
BsaWI w'CCGG_w

Cuts at:      0    2084    2292 
   Size:       2084     208
 
BseYI C'CCAG_C

Cuts at:      0     333    1174    1237    2292 
   Size:        333     841      63    1055

  Fragments arranged by size: 

                1055     841     333      63
 
BsiHKAI G_wGCw'C

Cuts at:      0    1216    2292 
   Size:       1216    1076
 
BsmI GAATG_Cn'

Cuts at:      0    1355    2292 
   Size:       1355     937
 
Bsp1286I G_dGCh'C

Cuts at:      0     866    1216    2292 
   Size:        866     350    1076

  Fragments arranged by size: 

                1076     866     350
 
BspHI T'CATG_A

Cuts at:      0    1418    2292 
   Size:       1418     874
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     892    1880    2292 
   Size:        892     988     412

  Fragments arranged by size: 

                 988     892     412
 
BsrDI GCAATG_nn'

Cuts at:      0     462    1046    2292 
   Size:        462     584    1246

  Fragments arranged by size: 

                1246     584     462
 
BsrFI r'CCGG_y

Cuts at:      0     200    2084    2292 
   Size:        200    1884     208

  Fragments arranged by size: 

                1884     208     200
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1045    1755    2292 
   Size:       1045     710     537

  Fragments arranged by size: 

                1045     710     537
 
BstBI TT'CG_AA

Cuts at:      0    1695    2292 
   Size:       1695     597
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1443    2292 
   Size:       1443     849
 
BtgI C'CryG_G

Cuts at:      0     377    2292 
   Size:        377    1915
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1020    2292 
   Size:       1020    1272
 
BtsI GCAGTG_nn'

Cuts at:      0    1061    1970    2292 
   Size:       1061     909     322

  Fragments arranged by size: 

                1061     909     322
 
ClaI AT'CG_AT

Cuts at:      0    1670    2005    2292 
   Size:       1670     335     287

  Fragments arranged by size: 

                1670     335     287
 
DraI TTT'AAA

Cuts at:      0     927     939    2292 
   Size:        927      12    1353

  Fragments arranged by size: 

                1353     927      12
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     702    2292 
   Size:        702    1590
 
EaeI y'GGCC_r

Cuts at:      0      32     257    2292 
   Size:         32     225    2035

  Fragments arranged by size: 

                2035     225      32
 
EarI CTCTTCn'nnn_

Cuts at:      0    1811    2292 
   Size:       1811     481
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1674    2292 
   Size:       1674     618
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1176    1970    2292 
   Size:       1176     794     322

  Fragments arranged by size: 

                1176     794     322
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1505    1920    1995    2292 
   Size:       1505     415      75     297

  Fragments arranged by size: 

                1505     415     297      75
 
EcoRI G'AATT_C

Cuts at:      0     877    1697    2292 
   Size:        877     820     595

  Fragments arranged by size: 

                 877     820     595
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     871    1770    2292 
   Size:        871     899     522

  Fragments arranged by size: 

                 899     871     522
 
HaeII r_GCGC'y

Cuts at:      0     843    1300    2292 
   Size:        843     457     992

  Fragments arranged by size: 

                 992     843     457
 
HincII GTy'rAC

Cuts at:      0     294     695    1794    2292 
   Size:        294     401    1099     498

  Fragments arranged by size: 

                1099     498     401     294
 
HindIII A'AGCT_T

Cuts at:      0    1718    2077    2292 
   Size:       1718     359     215

  Fragments arranged by size: 

                1718     359     215
 
KasI G'GCGC_C

Cuts at:      0    1296    2292 
   Size:       1296     996
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    2031    2292 
   Size:       2031     261
 
MscI TGG'CCA

Cuts at:      0      34    2292 
   Size:         34    2258
 
MslI CAynn'nnrTG

Cuts at:      0     376    1039    1258    2292 
   Size:        376     663     219    1034

  Fragments arranged by size: 

                1034     663     376     219
 
NarI GG'CG_CC

Cuts at:      0    1297    2292 
   Size:       1297     995
 
NsiI A_TGCA'T

Cuts at:      0     324    2292 
   Size:        324    1968
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     310    1108    2292 
   Size:        310     798    1184

  Fragments arranged by size: 

                1184     798     310
 
PfoI T'CCnGG_A

Cuts at:      0     966    2292 
   Size:        966    1326
 
PpuMI rG'GwC_Cy

Cuts at:      0    1505    1920    2292 
   Size:       1505     415     372

  Fragments arranged by size: 

                1505     415     372
 
PstI C_TGCA'G

Cuts at:      0    1745    2292 
   Size:       1745     547
 
SalI G'TCGA_C

Cuts at:      0     292    2292 
   Size:        292    2000
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1811    2292 
   Size:       1811     481
 
ScaI AGT'ACT

Cuts at:      0     737    1182    1844    2181    2292 
   Size:        737     445     662     337     111

  Fragments arranged by size: 

                 737     662     445     337     111
 
SexAI A'CCwGG_T

Cuts at:      0     782    2292 
   Size:        782    1510
 
SfcI C'TryA_G

Cuts at:      0    1741    2292 
   Size:       1741     551
 
SfoI GGC'GCC

Cuts at:      0    1298    2292 
   Size:       1298     994
 
SnaBI TAC'GTA

Cuts at:      0    2254    2292 
   Size:       2254      38
 
SspI AAT'ATT

Cuts at:      0     774    1539    2123    2292 
   Size:        774     765     584     169

  Fragments arranged by size: 

                 774     765     584     169
 
StuI AGG'CCT

Cuts at:      0    1460    2292 
   Size:       1460     832
 
StyI C'CwwG_G

Cuts at:      0    1130    2292 
   Size:       1130    1162
 
TatI w'GTAC_w

Cuts at:      0     735    1180    1786    1842    2179    2292 
   Size:        735     445     606      56     337     113

  Fragments arranged by size: 

                 735     606     445     337     113      56
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     587     619    2292 
   Size:        587      32    1673

  Fragments arranged by size: 

                1673     587      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     574    2292 
   Size:        574    1718
 
XmnI GAAnn'nnTTC

Cuts at:      0    1609    2292 
   Size:       1609     683
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AfeI     AgeI     ApoI     AvaI     BanI
     BbeI     BbsI    BciVI    BfrBI Bme1580I    BmgBI     BmrI     BpmI
    BsaAI    BsaBI    BsaHI    BsaWI    BseYI  BsiHKAI     BsmI Bsp1286I
    BspHI    BspMI    BsrDI    BsrFI   BstAPI    BstBI    BstXI     BtgI
    BtgZI     BtsI     ClaI     DraI     DrdI     EaeI     EarI     EciI
  Eco57MI EcoO109I    EcoRI     FalI    HaeII   HincII  HindIII     KasI
     MmeI     MscI     MslI     NarI     NsiI    PflMI     PfoI    PpuMI
     PstI     SalI     SapI     ScaI    SexAI     SfcI     SfoI    SnaBI
     SspI     StuI     StyI     TatI     TstI     XcmI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI    AflII   AflIII     AhdI     AleI     AlfI
     AloI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI    BanII    BbvCI     BcgI     BclI     BglI    BglII
     BlpI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaXI    BseRI
     BsgI    BsiEI    BsiWI    BsmBI    BspEI    BsrBI    BsrGI   BssHII
    BssSI   BstEII    BstYI  BstZ17I   Bsu36I    CspCI   DraIII     EagI
  EcoICRI    EcoNI    EcoRV     FseI     FspI    FspAI    Hin4I     HpaI
     KpnI     MfeI     MluI   MspA1I     NaeI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NspI     PacI     PasI     PciI     PmeI
     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI
    PvuII    RsrII     SacI    SacII    SanDI     SbfI     SfiI     SgfI
    SgrAI     SmaI     SmlI     SpeI     SphI     SrfI     SwaI    TaqII
  Tth111I     XbaI     XhoI     XmaI     ZraI
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