Restriction map for pF1KB6918
##################################
# NO CUT ENZYMES (  178 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AciI        
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwNI       Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp700I     
AspEI       AspHI       AspI        AvaI        AviII       
AvrII       BamHI       BbeI        BbrPI       BbsI        
BbvCI       BcgI        BciVI       BclI        BcnI        
BfaI        BfrI        BglI        BglII       BlnI        
BlpI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BseRI       
BsgI        BsiEI       BsiHKAI     BsiWI       BsmBI       
BsmI        BsoBI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstZ17I     
CelII       CfoI        Cfr10I      ClaI        CpoI        
DraIII      DrdI        DsaI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      EcoNI       EcoO65I     
EcoRI       EcoT22I     FauI        FbaI        FseI        
FspI        HaeII       HapII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HindIII     
HpaI        HpaII       KasI        KspI        MaeI        
MaeIII      MamI        MfeI        MluI        MroI        
MspA1I      MspI        MunI        MvnI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PleI        PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfcI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        SwaI        TaqI        Tsp45I      TspRI       
Tth111I     TthHB8I     XbaI        XcmI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

Acc65I        546  GGTACC               G^GTACC
AlwI           74  GATCC                GGATC(4/5)
AocI          424  CCTCAGG              CC^T[ACGT]AGG
Asp718I       546  GGTACC               G^GTACC
AvaII         429  GGTCC                G^G[AT]CC
BalI          372  TGGCCA               TGG^CCA
BanI          546  GGTACC               G^G[CT][GA]CC
BanII         177  GGGCTC               G[GA]GC[CT]^C
BbvI          604  GCTGC                GCAGC(8/12)
BmyI          177  GGGCTC               G[AGT]GC[ACT]^C
BpmI          599  CTGGAG               CTGGAG(16/14)
BsiYI         217  CCATTTTATGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          217  CCATTTTATGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I      177  GGGCTC               G[AGT]GC[ACT]^C
BsrDI         564  CATTGC               GCAATG(2/0)
BsrI          174  ACTGG                ACTGG(1/-1)
BstXI         329  CCAAAACTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI          73  AGATCC               [GA]^GATC[CT]
Bsu36I        424  CCTCAGG              CC^T[ACGT]AGG
Cfr13I        429  GGTCC                G^G[ACGT]CC
DraI          126  TTTAAA               TTT^AAA
DraII         428  AGGTCCC              [GA]G^G[ACGT]CC[CT]
EaeI          372  TGGCCA               [CT]^GGCC[GA]
Eco81I        424  CCTCAGG              CC^T[ACGT]AGG
EcoO109I      428  AGGTCCC              [GA]G^G[ACGT]CC[CT]
EcoRV           4  GATATC               GAT^ATC
EcoT14I       402  CCAAGG               C^C[AT][AT]GG
Fnu4HI        604  GCTGC                GC^[ACGT]GC
HinfI         259  GATTC                G^A[ACGT]TC
HphI          659  TCACC                GGTGA(8/7)
ItaI          604  GCTGC                GC^[ACGT]GC
KpnI          546  GGTACC               GGTAC^C
MaeII         540  ACGT                 A^CGT
MflI           73  AGATCC               [GA]^GATC[CT]
MluNI         372  TGGCCA               TGG^CCA
MscI          372  TGGCCA               TGG^CCA
MslI          135  CATGAAGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PflMI         217  CCATTTTATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI         428  AGGTCCC              [GA]G^G[AT]CC[CT]
Sau96I        429  GGTCC                G^G[ACGT]CC
SfaNI         613  GATGC                GCATC(5/9)
SmlI          195  CTTGAG               C^T[CT][GA]AG
StuI          152  AGGCCT               AGG^CCT
StyI          402  CCAAGG               C^C[AT][AT]GG
TfiI          259  GATTC                G^A[AT]TC
TseI          604  GCTGC                G^C[AT]GC
Van91I        217  CCATTTTATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII          73  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   13 enzymes) #
###################################

AfaI          538  GTAC                 GT^AC
              547  GTAC                
BsmAI         198  GAGAC                GTCTC(1/5)
              481  GAGAC               
BsmFI         430  GTCCC                GGGAC(10/14)
              642  GTCCC               
BstF5I        182  CATCC                GGATG(2/0)
              612  GGATG               
Cac8I         150  GCAGGC               GC[ACGT]^[ACGT]GC
              594  GCAGGC              
DdeI           95  CTCAG                C^T[ACGT]AG
              425  CTCAG               
EarI           69  GAAGAG               CTCTTC(1/4)
              478  GAAGAG              
FokI          182  CATCC                GGATG(9/13)
              612  GGATG               
HaeIII        153  GGCC                 GG^CC
              373  GGCC                
Ksp632I        69  GAAGAG               CTCTTC(1/4)
              478  GAAGAG              
MwoI          598  GCTGGAGCTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              607  GCCAGGGATGC         
NlaIV         429  GGTCCC               GG[ACGT]^[ACGT]CC
              546  GGTACC              
RsaI          538  GTAC                 GT^AC
              547  GTAC                


#########################################
# 3 OR MORE CUT ENZYMES (   19 enzymes) #
#########################################

AcsI        AluI        ApoI        BsaJI       BssKI       
BstNI       DpnII       EcoRII      MboI        MboII       
MnlI        MseI        MvaI        NdeII       NlaIII      
Sau3AI      ScrFI       Tru9I       Tsp509I     
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