Restriction map for pF1KB9928
##################################
# NO CUT ENZYMES (  119 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AclI        
AcyI        AflII       AgeI        AhdI        Alw44I      
AlwNI       AocI        ApaI        ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AviII       AvrII       BamHI       BbeI        BclI        
BfrI        BglII       BlnI        BlpI        Bpu1102I    
BsaBI       BsaHI       BsiWI       BsmBI       Bsp1407I    
BspDI       BspHI       BspLU11I    BspMI       BsrBI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstBI       BstEII      BstPI       BstUI       BstZ17I     
Bsu36I      CelII       Cfr10I      ClaI        CpoI        
DraI        DraIII      DrdI        Eam1105I    Eco81I      
EcoO65I     EcoRV       EcoT22I     FbaI        FseI        
FspI        HgaI        Hin1I       HindIII     HpaI        
KasI        KpnI        KspI        MamI        MfeI        
MluI        MunI        MvnI        NaeI        NarI        
NdeI        NgoMIV      NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflMI       PinAI       
PmeI        PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SbfI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        Sse8387I    
SspBI       SspI        SwaI        Van91I      XbaI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   57 enzymes) #
###################################

AccIII        321  TCCGGA               T^CCGGA
AflIII       1460  ACGTGT               A^C[GA][CT]GT
Aor51HI       514  AGCGCT               AGC^GCT
AspHI           3  GTGCTC               G[AT]GC[AT]^C
AspI         1223  GACAGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI         1299  CCCGAG               C^[CT]CG[GA]G
BalI         1812  TGGCCA               TGG^CCA
BanI         1466  GGTGCC               G^G[CT][GA]CC
BanII        1693  GAGCCC               G[GA]GC[CT]^C
BbrPI         300  CACGTG               CAC^GTG
BbsI          280  GAAGAC               GAAGAC(2/6)
BcgI          760  CGAAGAAACTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI         355  GGATAC               GTATCC(6/5)
BglI         1064  GCCTGGACGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI         280  GAAGAC               GAAGAC(2/6)
BsaAI         300  CACGTG               [CT]AC^GT[GA]
BseAI         321  TCCGGA               T^CCGGA
BsiEI        1071  CGGCCG               CG[GA][CT]^CG
BsiHKAI         3  GTGCTC               G[AT]GC[AT]^C
BsmFI          25  GGGAC                GGGAC(10/14)
BsmI           49  GAATGC               GAATGC(1/-1)
BsoBI        1299  CCCGAG               C^[CT]CG[GA]G
BspEI         321  TCCGGA               T^CCGGA
DraII        1352  AGGACCC              [GA]G^G[ACGT]CC[CT]
DsaI           60  CCATGG               C^C[GA][CT]GG
EagI         1071  CGGCCG               C^GGCCG
EarI          967  GAAGAG               CTCTTC(1/4)
EclXI        1071  CGGCCG               C^GGCCG
Eco47III      514  AGCGCT               AGC^GCT
Eco52I       1071  CGGCCG               C^GGCCG
EcoNI        1701  CCTCTCCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I     1352  AGGACCC              [GA]G^G[ACGT]CC[CT]
EcoRI         423  GAATTC               G^AATTC
HincII       1099  GTTGAC               GT[CT]^[GA]AC
HindII       1099  GTTGAC               GT[CT]^[GA]AC
Ksp632I       967  GAAGAG               CTCTTC(1/4)
MluNI        1812  TGGCCA               TGG^CCA
MroI          321  TCCGGA               T^CCGGA
MscI         1812  TGGCCA               TGG^CCA
MseI          374  TTAA                 T^TAA
MspA1I        850  CAGCTG               C[AC]G^C[GT]G
NcoI           60  CCATGG               C^CATGG
NheI          511  GCTAGC               G^CTAGC
NspI          490  GCATGC               [GA]CATG^[CT]
PflFI        1223  GACAGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI         300  CACGTG               CAC^GTG
PmlI          300  CACGTG               CAC^GTG
PpuMI        1352  AGGACCC              [GA]G^G[AT]CC[CT]
PstI         1186  CTGCAG               CTGCA^G
PvuII         850  CAGCTG               CAG^CTG
SapI          967  GAAGAGC              GCTCTTC(1/4)
ScaI         1316  AGTACT               AGT^ACT
SfcI         1186  CTGCAG               C^T[GA][CT]AG
SmlI         1348  CTCAAG               C^T[CT][GA]AG
SphI          490  GCATGC               GCATG^C
Tru9I         374  TTAA                 T^TAA
Tth111I      1223  GACAGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AcsI          423  GAATTC               [GA]^AATT[CT]
              801  AAATTT              
AlwI         1848  GATCC                GGATC(4/5)
             1150  GGATC               
ApoI          423  GAATTC               [GA]^AATT[CT]
              801  AAATTT              
BbvCI        1440  CCTCAGC              CCTCAGC(-5/-2)
             1377  GCTGAGG             
BmyI            3  GTGCTC               G[AGT]GC[ACT]^C
             1693  GAGCCC              
BsaWI         321  TCCGGA               [AT]^CCGG[AT]
              459  TCCGGT              
BsgI          996  GTGCAG               GTGCAG(16/14)
             1726  GTGCAG              
Bsp1286I        3  GTGCTC               G[AGT]GC[ACT]^C
             1693  GAGCCC              
BsrDI         439  CATTGC               GCAATG(2/0)
             1566  CATTGC              
BstYI        1150  GGATCT               [GA]^GATC[CT]
             1847  AGATCC              
EaeI         1071  CGGCCG               [CT]^GGCC[GA]
             1812  TGGCCA              
FauI          159  CCCGC                CCCGC(4/6)
             1852  CCCGC               
HaeII         155  AGCGCC               [GA]GCGC^[CT]
              514  AGCGCT              
HphI          948  GGTGA                GGTGA(8/7)
             1553  TCACC               
MflI         1150  GGATCT               [GA]^GATC[CT]
             1847  AGATCC              
XcmI          174  CCAGGAGAATGATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              911  CCAAAAATAAGCTGG     
XhoII        1150  GGATCT               [GA]^GATC[CT]
             1847  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   65 enzymes) #
#########################################

AciI        AfaI        AluI        AvaII       BbvI        
BcnI        BfaI        BpmI        BsaI        BsaJI       
BseRI       BsiYI       BslI        BsmAI       BsrI        
BssKI       BstAPI      BstF5I      BstNI       BstXI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
EcoRII      EcoT14I     Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HinfI       HpaII       
ItaI        MaeI        MaeII       MaeIII      MboI        
MboII       MnlI        MslI        MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
PleI        RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       StuI        StyI        TaqI        TfiI        
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
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