Restriction map for pF1KB6307
##################################
# NO CUT ENZYMES (  155 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AvaI        AviII       AvrII       
BalI        BamHI       BbrPI       BbsI        BcgI        
BciVI       BclI        BfrI        BglI        BglII       
BlnI        BpuAI       BsaAI       BsaBI       BsaI        
BseAI       BseRI       BsgI        BsiHKAI     BsiWI       
BsmBI       BsoBI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstXI       
BstZ17I     Bsu36I      Cfr10I      ClaI        CpoI        
DraI        DraIII      DrdI        EaeI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoO65I     EcoRI       EcoRV       EcoT14I     EcoT22I     
FbaI        FseI        FspI        HincII      HindII      
HindIII     HpaI        KpnI        MamI        MfeI        
MluI        MluNI       MroI        MscI        MseI        
MslI        MunI        NaeI        NcoI        NdeI        
NgoMIV      NheI        NlaIII      NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PstI        PvuI        
PvuII       RcaI        RsrII       SacI        SalI        
SapI        SbfI        SexAI       SfcI        SfiI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StyI        SwaI        Tru9I       Tth111I     XbaI        
XcmI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AcsI          325  GAATTT               [GA]^AATT[CT]
AlwI          489  GGATC                GGATC(4/5)
AlwNI         545  CAGTGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApoI          325  GAATTT               [GA]^AATT[CT]
AvaII          18  GGACC                G^G[AT]CC
BanII         393  GAGCCC               G[GA]GC[CT]^C
BbeI           45  GGCGCC               GGCGC^C
BbvCI         336  GCTGAGG              CCTCAGC(-5/-2)
BfaI          472  CTAG                 C^TAG
BlpI          562  GCTAAGC              GC^T[ACGT]AGC
BmyI          393  GAGCCC               G[AGT]GC[ACT]^C
BpmI          716  CTCCAG               CTGGAG(16/14)
Bpu1102I      562  GCTAAGC              GC^T[ACGT]AGC
BsaWI          51  TCCGGT               [AT]^CCGG[AT]
BsiEI          33  CGACCG               CG[GA][CT]^CG
BsmAI         291  GAGAC                GTCTC(1/5)
BsmI          665  GCATTC               GAATGC(1/-1)
Bsp1286I      393  GAGCCC               G[AGT]GC[ACT]^C
BsrI          544  CCAGT                ACTGG(1/-1)
BstYI         489  GGATCT               [GA]^GATC[CT]
CelII         562  GCTAAGC              GC^T[ACGT]AGC
DpnII         490  GATC                 ^GATC
DraII          17  GGGACCC              [GA]G^G[ACGT]CC[CT]
DsaI          260  CCGCGG               C^C[GA][CT]GG
EarI          553  GAAGAG               CTCTTC(1/4)
EcoNI         630  CCTCAAAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I       17  GGGACCC              [GA]G^G[ACGT]CC[CT]
HaeII          45  GGCGCC               [GA]GCGC^[CT]
HphI          198  GGTGA                GGTGA(8/7)
KasI           45  GGCGCC               G^GCGCC
Ksp632I       553  GAAGAG               CTCTTC(1/4)
KspI          260  CCGCGG               CCGC^GG
MaeI          472  CTAG                 C^TAG
MaeII         413  ACGT                 A^CGT
MaeIII        305  GTGAC                ^GT[ACGT]AC
MboI          490  GATC                 ^GATC
MflI          489  GGATCT               [GA]^GATC[CT]
NarI           45  GGCGCC               GG^CGCC
NdeII         490  GATC                 ^GATC
PflMI         740  CCAAGTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI          17  GGGACCC              [GA]G^G[AT]CC[CT]
SacII         260  CCGCGG               CCGC^GG
Sau3AI        490  GATC                 ^GATC
ScaI          758  AGTACT               AGT^ACT
SfoI           45  GGCGCC               GGC^GCC
StuI          340  AGGCCT               AGG^CCT
TfiI          438  GATTC                G^A[AT]TC
Tsp45I        305  GTGAC                ^GT[GC]AC
Tsp509I       326  AATT                 ^AATT
Van91I        740  CCAAGTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII         489  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (    9 enzymes) #
###################################

AcyI           45  GGCGCC               G[GA]^CG[CT]C
              145  GGCGTC              
AfaI          231  GTAC                 GT^AC
              759  GTAC                
BcnI          106  CCGGG                CC^[GC]GG
              114  CCGGG               
BsaHI          45  GGCGCC               G[GA]^CG[CT]C
              145  GGCGTC              
Hin1I          45  GGCGCC               G[GA]^CG[CT]C
              145  GGCGTC              
MspA1I         36  CCGCTG               C[AC]G^C[GT]G
              260  CCGCGG              
NciI          106  CCGGG                CC^[GC]GG
              114  CCGGG               
RsaI          231  GTAC                 GT^AC
              759  GTAC                
SfaNI         443  GATGC                GCATC(5/9)
              646  GATGC               


#########################################
# 3 OR MORE CUT ENZYMES (   43 enzymes) #
#########################################

AccII       AciI        AluI        BanI        BbvI        
BsaJI       BsiYI       BslI        BsmFI       BssKI       
BstF5I      BstNI       BstUI       Cac8I       CfoI        
Cfr13I      DdeI        EcoRII      FauI        Fnu4HI      
FokI        HaeIII      HapII       HgaI        HhaI        
HinP1I      HinfI       HpaII       ItaI        MboII       
MnlI        MspI        MvaI        MvnI        MwoI        
NlaIV       PleI        Sau96I      ScrFI       TaqI        
TseI        TspRI       TthHB8I     
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