Restriction map for pF1KE0964
##################################
# NO CUT ENZYMES (  152 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AclI        
AcyI        AflII       AgeI        AhdI        Alw44I      
AocI        ApaI        ApaLI       AscI        AseI        
AsnI        AspEI       AspI        AvaI        AviII       
AvrII       BalI        BamHI       BbeI        BbrPI       
BbvCI       BcgI        BclI        BcnI        BfrI        
BglI        BlnI        BlpI        Bpu1102I    BsaAI       
BsaHI       BsaI        BsaWI       BseAI       BsiEI       
BsiWI       BsmBI       BsmI        BsoBI       Bsp1407I    
BspDI       BspEI       BspHI       BspMI       BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstXI       BstZ17I     Bsu36I      CelII       
Cfr10I      ClaI        CpoI        DraIII      DrdI        
DsaI        EaeI        EagI        Eam1105I    EclXI       
Eco52I      Eco81I      EcoO65I     EcoRV       EcoT14I     
EcoT22I     FauI        FbaI        FseI        FspI        
HapII       HgaI        Hin1I       HindIII     HpaI        
HpaII       KasI        KspI        MfeI        MluI        
MluNI       MroI        MscI        MspA1I      MspI        
MunI        MvnI        MwoI        NaeI        NarI        
NciI        NcoI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PstI        PvuI        
PvuII       RcaI        RsrII       SacII       SalI        
SapI        SbfI        ScaI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
Sse8387I    SspBI       SspI        StuI        StyI        
SwaI        Tth111I     XbaI        XcmI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

Acc65I        406  GGTACC               G^GTACC
AciI          723  GCGG                 CCGC(-3/-1)
AflIII        171  ACATGT               A^C[GA][CT]GT
Aor51HI      1105  AGCGCT               AGC^GCT
Asp700I       481  GAATATGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I       406  GGTACC               G^GTACC
BanI          406  GGTACC               G^G[CT][GA]CC
BbsI         1196  GAAGAC               GAAGAC(2/6)
BciVI         440  GTATCC               GTATCC(6/5)
BpmI          110  CTGGAG               CTGGAG(16/14)
BpuAI        1196  GAAGAC               GAAGAC(2/6)
BsaBI        1337  GATCTCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI        1141  GAGGAG               GAGGAG(10/8)
BspLU11I      171  ACATGT               A^CATGT
Cac8I         716  GCATGC               GC[ACGT]^[ACGT]GC
CfoI         1106  GCGC                 GCG^C
DraI          412  TTTAAA               TTT^AAA
DraII         764  AGGACCC              [GA]G^G[ACGT]CC[CT]
EarI         1441  GAAGAG               CTCTTC(1/4)
Eco47III     1105  AGCGCT               AGC^GCT
EcoNI         616  CCTAGAAAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      764  AGGACCC              [GA]G^G[ACGT]CC[CT]
HaeII        1105  AGCGCT               [GA]GCGC^[CT]
HhaI         1106  GCGC                 GCG^C
HinP1I       1106  GCGC                 G^CGC
HphI          493  GGTGA                GGTGA(8/7)
KpnI          406  GGTACC               GGTAC^C
Ksp632I      1441  GAAGAG               CTCTTC(1/4)
MaeII         695  ACGT                 A^CGT
MamI         1337  GATCTCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI          896  CATATG               CA^TATG
PflMI         867  CCAATTTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI         764  AGGACCC              [GA]G^G[AT]CC[CT]
SacI          808  GAGCTC               GAGCT^C
SexAI         539  ACCAGGT              A^CC[AT]GGT
SfcI          570  CTACAG               C^T[GA][CT]AG
SphI          716  GCATGC               GCATG^C
TaqI         1079  TCGA                 T^CGA
TfiI         1027  GATTC                G^A[AT]TC
Tsp45I        692  GTGAC                ^GT[GC]AC
TthHB8I      1079  TCGA                 T^CGA
Van91I        867  CCAATTTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI          481  GAATATGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AlwNI         754  CAGAACCTG            CAG[ACGT][ACGT][ACGT]^CTG
              849  CAGTTGCTG           
AspHI         808  GAGCTC               G[AT]GC[AT]^C
              881  GTGCTC              
AvaII         765  GGACC                G^G[AT]CC
              961  GGACC               
BanII         808  GAGCTC               G[GA]GC[CT]^C
              833  GGGCTC              
BfaI          370  CTAG                 C^TAG
              617  CTAG                
BglII         389  AGATCT               A^GATCT
             1336  AGATCT              
BsgI          778  GTGCAG               GTGCAG(16/14)
             1132  GTGCAG              
BsiHKAI       808  GAGCTC               G[AT]GC[AT]^C
              881  GTGCTC              
BsmAI        1444  GAGAC                GTCTC(1/5)
             1215  GTCTC               
BsmFI         634  GGGAC                GGGAC(10/14)
              581  GTCCC               
BssKI         540  CCAGG                ^CC[ACGT]GG
              746  CCTGG               
BstNI         540  CCAGG                CC^[AT]GG
              746  CCTGG               
EcoRI         296  GAATTC               G^AATTC
              328  GAATTC              
EcoRII        540  CCAGG                ^CC[AT]GG
              746  CCTGG               
HincII        420  GTCAAC               GT[CT]^[GA]AC
             1054  GTCAAC              
HindII        420  GTCAAC               GT[CT]^[GA]AC
             1054  GTCAAC              
MaeI          370  CTAG                 C^TAG
              617  CTAG                
MslI          472  CATGAAAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              490  CATGGTGATG          
MvaI          540  CCAGG                CC^[AT]GG
              746  CCTGG               
NspI          171  ACATGT               [GA]CATG^[CT]
              716  GCATGC              
PleI         1111  GAGTC                GAGTC(4/5)
             1213  GAGTC               
ScrFI         540  CCAGG                CC^[ACGT]GG
              746  CCTGG               
SmlI          925  CTCAAG               C^T[CT][GA]AG
             1217  CTCAAG              


#########################################
# 3 OR MORE CUT ENZYMES (   40 enzymes) #
#########################################

AcsI        AfaI        AluI        AlwI        ApoI        
BbvI        BmyI        BsaJI       BsiYI       BslI        
Bsp1286I    BsrI        BstF5I      BstYI       Cfr13I      
DdeI        DpnII       Fnu4HI      FokI        HaeIII      
HinfI       ItaI        MaeIII      MboI        MboII       
MflI        MnlI        MseI        NdeII       NlaIII      
NlaIV       RsaI        Sau3AI      Sau96I      SfaNI       
Tru9I       TseI        Tsp509I     TspRI       XhoII       
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