Restriction map for
pF1KB9967
(Linear) (Six-base) MAPSORT of: KIBB9967.seq Check: 4484 from: 1 to: 1653
KIBB9967.orf
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AatII G_ACGT'C
Cuts at: 0 368 1653
Size: 368 1285
AccI GT'mk_AC
Cuts at: 0 368 1653
Size: 368 1285
Acc65I G'GTAC_C
Cuts at: 0 1251 1653
Size: 1251 402
AclI AA'CG_TT
Cuts at: 0 512 1653
Size: 512 1141
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 531 899 985 1653
Size: 531 368 86 668
Fragments arranged by size:
668 531 368 86
AleI CACnn'nnGTG
Cuts at: 0 382 1653
Size: 382 1271
AlwNI CAG_nnn'CTG
Cuts at: 0 182 764 1653
Size: 182 582 889
Fragments arranged by size:
889 582 182
ApaI G_GGCC'C
Cuts at: 0 47 1653
Size: 47 1606
ApaLI G'TGCA_C
Cuts at: 0 705 1653
Size: 705 948
ApoI r'AATT_y
Cuts at: 0 659 1142 1300 1653
Size: 659 483 158 353
Fragments arranged by size:
659 483 353 158
AvaI C'yCGr_G
Cuts at: 0 283 1653
Size: 283 1370
BanI G'GyrC_C
Cuts at: 0 228 423 792 1251 1653
Size: 228 195 369 459 402
Fragments arranged by size:
459 402 369 228 195
BanII G_rGCy'C
Cuts at: 0 47 1352 1653
Size: 47 1305 301
Fragments arranged by size:
1305 301 47
BbsI GAAGACnn'nnnn_
Cuts at: 0 1329 1653
Size: 1329 324
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 130 164 1653
Size: 130 34 1489
Fragments arranged by size:
1489 130 34
BglI GCCn_nnn'nGGC
Cuts at: 0 344 1653
Size: 344 1309
BglII A'GATC_T
Cuts at: 0 620 1585 1653
Size: 620 965 68
Fragments arranged by size:
965 620 68
BlpI GC'TnA_GC
Cuts at: 0 73 1346 1653
Size: 73 1273 307
Fragments arranged by size:
1273 307 73
Bme1580I G_kGCm'C
Cuts at: 0 47 233 428 709 1653
Size: 47 186 195 281 944
Fragments arranged by size:
944 281 195 186 47
BmgBI CAC'GTC
Cuts at: 0 306 1653
Size: 306 1347
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 310 343 1386 1653
Size: 310 33 1043 267
Fragments arranged by size:
1043 310 267 33
Bpu10I CC'TnA_GC
Cuts at: 0 172 1653
Size: 172 1481
BsaBI GATnn'nnATC
Cuts at: 0 1501 1653
Size: 1501 152
BsaHI Gr'CG_yC
Cuts at: 0 161 365 1653
Size: 161 204 1288
Fragments arranged by size:
1288 204 161
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1214 1244 1653
Size: 1214 30 409
Fragments arranged by size:
1214 409 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 451 964 1238 1653
Size: 451 513 274 415
Fragments arranged by size:
513 451 415 274
BseYI C'CCAG_C
Cuts at: 0 347 575 1293 1653
Size: 347 228 718 360
Fragments arranged by size:
718 360 347 228
BsiEI CG_ry'CG
Cuts at: 0 222 1653
Size: 222 1431
BsiHKAI G_wGCw'C
Cuts at: 0 143 461 709 1352 1653
Size: 143 318 248 643 301
Fragments arranged by size:
643 318 301 248 143
BsmI GAATG_Cn'
Cuts at: 0 1463 1648 1653
Size: 1463 185 5
Fragments arranged by size:
1463 185 5
Bsp1286I G_dGCh'C
Cuts at: 0 47 143 233 428 461 709 1352 1653
Size: 47 96 90 195 33 248 643 301
Fragments arranged by size:
643 301 248 195 96 90 47 33
BsrDI GCAATG_nn'
Cuts at: 0 1418 1653
Size: 1418 235
BsrFI r'CCGG_y
Cuts at: 0 251 1653
Size: 251 1402
BsrGI T'GTAC_A
Cuts at: 0 534 1653
Size: 534 1119
BstXI CCAn_nnnn'nTGG
Cuts at: 0 633 1284 1653
Size: 633 651 369
Fragments arranged by size:
651 633 369
BstYI r'GATC_y
Cuts at: 0 620 1585 1653
Size: 620 965 68
Fragments arranged by size:
965 620 68
BtgI C'CryG_G
Cuts at: 0 527 1394 1653
Size: 527 867 259
Fragments arranged by size:
867 527 259
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 249 1653
Size: 249 1404
BtsI GCAGTG_nn'
Cuts at: 0 935 1340 1653
Size: 935 405 313
Fragments arranged by size:
935 405 313
DrdI GACnn_nn'nnGTC
Cuts at: 0 1029 1653
Size: 1029 624
EaeI y'GGCC_r
Cuts at: 0 219 1653
Size: 219 1434
EagI C'GGCC_G
Cuts at: 0 219 1653
Size: 219 1434
EarI CTCTTCn'nnn_
Cuts at: 0 1243 1547 1653
Size: 1243 304 106
Fragments arranged by size:
1243 304 106
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 783 1653
Size: 783 870
EcoICRI GAG'CTC
Cuts at: 0 1350 1653
Size: 1350 303
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 310 343 531 899 985 1386 1653
Size: 310 33 188 368 86 401 267
Fragments arranged by size:
401 368 310 267 188 86 33
EcoO109I rG'GnC_Cy
Cuts at: 0 43 44 131 1653
Size: 43 1 87 1522
Fragments arranged by size:
1522 87 43 1
EcoRV GAT'ATC
Cuts at: 0 854 885 1653
Size: 854 31 768
Fragments arranged by size:
854 768 31
HaeII r_GCGC'y
Cuts at: 0 57 80 182 283 787 1653
Size: 57 23 102 101 504 866
Fragments arranged by size:
866 504 102 101 57 23
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1214 1246 1653
Size: 1214 32 407
Fragments arranged by size:
1214 407 32
HindIII A'AGCT_T
Cuts at: 0 977 1653
Size: 977 676
KpnI G_GTAC'C
Cuts at: 0 1255 1653
Size: 1255 398
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 650 1653
Size: 650 1003
MslI CAynn'nnrTG
Cuts at: 0 382 1103 1378 1474 1653
Size: 382 721 275 96 179
Fragments arranged by size:
721 382 275 179 96
MspA1I CmG'CkG
Cuts at: 0 146 204 239 321 486 575 816 1653
Size: 146 58 35 82 165 89 241 837
Fragments arranged by size:
837 241 165 146 89 82 58 35
NcoI C'CATG_G
Cuts at: 0 527 1653
Size: 527 1126
PflMI CCAn_nnn'nTGG
Cuts at: 0 575 1653
Size: 575 1078
PpuMI rG'GwC_Cy
Cuts at: 0 131 1653
Size: 131 1522
PspOMI G'GGCC_C
Cuts at: 0 43 1653
Size: 43 1610
PstI C_TGCA'G
Cuts at: 0 244 560 1209 1653
Size: 244 316 649 444
Fragments arranged by size:
649 444 316 244
PvuII CAG'CTG
Cuts at: 0 239 321 486 575 1653
Size: 239 82 165 89 1078
Fragments arranged by size:
1078 239 165 89 82
SacI G_AGCT'C
Cuts at: 0 1352 1653
Size: 1352 301
SanDI GG'GwC_CC
Cuts at: 0 131 1653
Size: 131 1522
ScaI AGT'ACT
Cuts at: 0 1211 1653
Size: 1211 442
SfcI C'TryA_G
Cuts at: 0 240 556 1205 1653
Size: 240 316 649 448
Fragments arranged by size:
649 448 316 240
SspI AAT'ATT
Cuts at: 0 1011 1653
Size: 1011 642
StyI C'CwwG_G
Cuts at: 0 527 1631 1653
Size: 527 1104 22
Fragments arranged by size:
1104 527 22
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 403 1653
Size: 403 1250
TatI w'GTAC_w
Cuts at: 0 534 1209 1653
Size: 534 675 444
Fragments arranged by size:
675 534 444
XmnI GAAnn'nnTTC
Cuts at: 0 979 1120 1579 1653
Size: 979 141 459 74
Fragments arranged by size:
979 459 141 74
ZraI GAC'GTC
Cuts at: 0 366 1653
Size: 366 1287
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AleI AlwNI ApaI
ApaLI ApoI AvaI BanI BanII BbsI BcgI BglI
BglII BlpI Bme1580I BmgBI BpmI Bpu10I BsaBI BsaHI
BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BsrDI
BsrFI BsrGI BstXI BstYI BtgI BtgZI BtsI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRV
HaeII Hin4I HindIII KpnI MmeI MslI MspA1I NcoI
PflMI PpuMI PspOMI PstI PvuII SacI SanDI ScaI
SfcI SspI StyI TaqII TatI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AflIII AgeI AhdI AlfI AloI
AscI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI
BciVI BclI BfrBI BmrI BmtI BplI BpuEI BsaI
BsaAI BsaWI BsgI BsiWI BsmBI BspEI BspHI BspMI
BsrBI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I
ClaI CspCI DraI DraIII EcoNI EcoRI FalI FseI
FspI FspAI HincII HpaI KasI MfeI MluI MscI
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PasI PciI PfoI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI
SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI StuI SwaI TstI
Tth111I XbaI XcmI XhoI XmaI