Restriction map for
pF1KB7644
(Linear) (Six-base) MAPSORT of: KIBB7644.seq Check: 4255 from: 1 to: 1170
KIBB7644.orf
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 341 1170
Size: 341 829
AccI GT'mk_AC
Cuts at: 0 224 326 1170
Size: 224 102 844
Fragments arranged by size:
844 224 102
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 664 1170
Size: 148 516 506
Fragments arranged by size:
516 506 148
AfeI AGC'GCT
Cuts at: 0 250 1170
Size: 250 920
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 359 1170
Size: 359 811
AlwNI CAG_nnn'CTG
Cuts at: 0 146 158 463 1053 1170
Size: 146 12 305 590 117
Fragments arranged by size:
590 305 146 117 12
ApoI r'AATT_y
Cuts at: 0 385 785 1170
Size: 385 400 385
Fragments arranged by size:
400 385 385
BanI G'GyrC_C
Cuts at: 0 662 1170
Size: 662 508
BanII G_rGCy'C
Cuts at: 0 47 733 919 1170
Size: 47 686 186 251
Fragments arranged by size:
686 251 186 47
BbsI GAAGACnn'nnnn_
Cuts at: 0 431 1170
Size: 431 739
BciVI GTATCCnnnnn_n'
Cuts at: 0 295 1170
Size: 295 875
BfrBI ATG'CAT
Cuts at: 0 523 1170
Size: 523 647
BglI GCCn_nnn'nGGC
Cuts at: 0 549 1170
Size: 549 621
BlpI GC'TnA_GC
Cuts at: 0 470 766 1170
Size: 470 296 404
Fragments arranged by size:
470 404 296
BmrI ACTGGGnnnn_n'
Cuts at: 0 78 927 1170
Size: 78 849 243
Fragments arranged by size:
849 243 78
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 192 485 526 1170
Size: 192 293 41 644
Fragments arranged by size:
644 293 192 41
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 924 1170
Size: 148 776 246
Fragments arranged by size:
776 246 148
BsaAI yAC'GTr
Cuts at: 0 555 1170
Size: 555 615
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 517 547 553 583 1170
Size: 517 30 6 30 587
Fragments arranged by size:
587 517 30 30 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 711 1170
Size: 711 459
BseYI C'CCAG_C
Cuts at: 0 214 668 1066 1170
Size: 214 454 398 104
Fragments arranged by size:
454 398 214 104
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 191 319 1170
Size: 191 128 851
Fragments arranged by size:
851 191 128
BsiHKAI G_wGCw'C
Cuts at: 0 733 1170
Size: 733 437
BsmI GAATG_Cn'
Cuts at: 0 525 1170
Size: 525 645
Bsp1286I G_dGCh'C
Cuts at: 0 47 733 919 1170
Size: 47 686 186 251
Fragments arranged by size:
686 251 186 47
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 341 1170
Size: 341 829
BsrDI GCAATG_nn'
Cuts at: 0 1019 1170
Size: 1019 151
BsrFI r'CCGG_y
Cuts at: 0 362 1170
Size: 362 808
BstAPI GCAn_nnn'nTGC
Cuts at: 0 263 272 356 1170
Size: 263 9 84 814
Fragments arranged by size:
814 263 84 9
BstXI CCAn_nnnn'nTGG
Cuts at: 0 91 504 1139 1170
Size: 91 413 635 31
Fragments arranged by size:
635 413 91 31
BtgI C'CryG_G
Cuts at: 0 23 374 872 1061 1170
Size: 23 351 498 189 109
Fragments arranged by size:
498 351 189 109 23
BtsI GCAGTG_nn'
Cuts at: 0 336 1107 1170
Size: 336 771 63
Fragments arranged by size:
771 336 63
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 281 316 1170
Size: 281 35 854
Fragments arranged by size:
854 281 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 595 1170
Size: 595 575
EaeI y'GGCC_r
Cuts at: 0 371 801 1170
Size: 371 430 369
Fragments arranged by size:
430 371 369
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 208 860 1170
Size: 208 652 310
Fragments arranged by size:
652 310 208
EcoICRI GAG'CTC
Cuts at: 0 731 1170
Size: 731 439
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 192 485 526 664 1170
Size: 148 44 293 41 138 506
Fragments arranged by size:
506 293 148 138 44 41
EcoNI CCTnn'n_nnAGG
Cuts at: 0 397 646 1170
Size: 397 249 524
Fragments arranged by size:
524 397 249
EcoO109I rG'GnC_Cy
Cuts at: 0 132 1072 1170
Size: 132 940 98
Fragments arranged by size:
940 132 98
EcoRI G'AATT_C
Cuts at: 0 385 1170
Size: 385 785
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 255 1170
Size: 255 915
FspI TGC'GCA
Cuts at: 0 1025 1170
Size: 1025 145
HaeII r_GCGC'y
Cuts at: 0 252 1170
Size: 252 918
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 553 585 1170
Size: 553 32 585
Fragments arranged by size:
585 553 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1147 1170
Size: 1147 23
MscI TGG'CCA
Cuts at: 0 373 803 1170
Size: 373 430 367
Fragments arranged by size:
430 373 367
MspA1I CmG'CkG
Cuts at: 0 25 189 369 504 567 668 1170
Size: 25 164 180 135 63 101 502
Fragments arranged by size:
502 180 164 135 101 63 25
NcoI C'CATG_G
Cuts at: 0 872 1061 1170
Size: 872 189 109
Fragments arranged by size:
872 189 109
NsiI A_TGCA'T
Cuts at: 0 525 1170
Size: 525 645
NspI r_CATG'y
Cuts at: 0 1030 1170
Size: 1030 140
PfoI T'CCnGG_A
Cuts at: 0 47 110 134 1170
Size: 47 63 24 1036
Fragments arranged by size:
1036 63 47 24
PmlI CAC'GTG
Cuts at: 0 555 1170
Size: 555 615
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 439 471 1170
Size: 439 32 699
Fragments arranged by size:
699 439 32
PpuMI rG'GwC_Cy
Cuts at: 0 132 1072 1170
Size: 132 940 98
Fragments arranged by size:
940 132 98
PstI C_TGCA'G
Cuts at: 0 738 1170
Size: 738 432
PvuII CAG'CTG
Cuts at: 0 189 369 504 567 1170
Size: 189 180 135 63 603
Fragments arranged by size:
603 189 180 135 63
SacI G_AGCT'C
Cuts at: 0 733 1170
Size: 733 437
SacII CC_GC'GG
Cuts at: 0 26 1170
Size: 26 1144
SanDI GG'GwC_CC
Cuts at: 0 1072 1170
Size: 1072 98
SfcI C'TryA_G
Cuts at: 0 734 1170
Size: 734 436
SmlI C'TyrA_G
Cuts at: 0 163 903 1170
Size: 163 740 267
Fragments arranged by size:
740 267 163
SphI G_CATG'C
Cuts at: 0 1030 1170
Size: 1030 140
StuI AGG'CCT
Cuts at: 0 584 1170
Size: 584 586
StyI C'CwwG_G
Cuts at: 0 872 1061 1170
Size: 872 189 109
Fragments arranged by size:
872 189 109
Tth111I GACn'n_nGTC
Cuts at: 0 322 1170
Size: 322 848
Enzymes that do cut:
AarI AccI AcuI AfeI AlfI AlwNI ApoI BanI
BanII BbsI BciVI BfrBI BglI BlpI BmrI BpmI
BpuEI BsaAI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
Bsp1286I BspMI BsrDI BsrFI BstAPI BstXI BtgI BtsI
CspCI DrdI EaeI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I MmeI MscI MspA1I
NcoI NsiI NspI PfoI PmlI PpiI PpuMI PstI
PvuII SacI SacII SanDI SfcI SmlI SphI StuI
StyI Tth111I
Enzymes that do not cut:
AatII Acc65I AclI AflII AflIII AgeI AhdI AleI
AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BbeI BbvCI BcgI BclI BglII Bme1580I
BmgBI BmtI BplI Bpu10I BsaI BsaBI BsaHI BsaWI
BsiEI BsiWI BsmBI BspEI BspHI BsrBI BsrGI BssHII
BssSI BstBI BstEII BstYI BstZ17I Bsu36I BtgZI ClaI
DraI DraIII EagI EarI EcoRV FseI FspAI HincII
HindIII HpaI KasI KpnI MfeI MluI MslI NaeI
NarI NdeI NgoMIV NheI NotI NruI PacI PasI
PciI PflMI PmeI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SalI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI
SwaI TaqII TatI TstI XbaI XcmI XhoI XmaI
XmnI ZraI