Restriction map for pF1KE1052
 (Linear) (Six-base) MAPSORT of: KIEE1052.seq  Check: 3484  from: 1  to: 1125



KIEE1052.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:19  ..

AccI GT'mk_AC

Cuts at:      0     551    1125 
   Size:        551     574
 
AfeI AGC'GCT

Cuts at:      0      44     370    1125 
   Size:         44     326     755

  Fragments arranged by size: 

                 755     326      44
 
AleI CACnn'nnGTG

Cuts at:      0     722    1125 
   Size:        722     403
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     895     927    1125 
   Size:        895      32     198

  Fragments arranged by size: 

                 895     198      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     428    1125 
   Size:        428     697
 
ApaLI G'TGCA_C

Cuts at:      0      34    1125 
   Size:         34    1091
 
ApoI r'AATT_y

Cuts at:      0     143     418     628    1125 
   Size:        143     275     210     497

  Fragments arranged by size: 

                 497     275     210     143
 
BanII G_rGCy'C

Cuts at:      0     484     896    1125 
   Size:        484     412     229

  Fragments arranged by size: 

                 484     412     229
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      16    1040    1125 
   Size:         16    1024      85

  Fragments arranged by size: 

                1024      85      16
 
BbvCI CC'TCA_GC

Cuts at:      0     349    1125 
   Size:        349     776
 
BglII A'GATC_T

Cuts at:      0     757     764    1125 
   Size:        757       7     361

  Fragments arranged by size: 

                 757     361       7
 
Bme1580I G_kGCm'C

Cuts at:      0      38    1125 
   Size:         38    1087
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     797    1000    1125 
   Size:        797     203     125

  Fragments arranged by size: 

                 797     203     125
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      67    1125 
   Size:         67    1058
 
Bpu10I CC'TnA_GC

Cuts at:      0     349    1125 
   Size:        349     776
 
BsaAI yAC'GTr

Cuts at:      0     792    1125 
   Size:        792     333
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     895     925    1125 
   Size:        895      30     200

  Fragments arranged by size: 

                 895     200      30
 
BseYI C'CCAG_C

Cuts at:      0     428     532     633     648    1125 
   Size:        428     104     101      15     477

  Fragments arranged by size: 

                 477     428     104     101      15
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     454     861    1125 
   Size:        454     407     264

  Fragments arranged by size: 

                 454     407     264
 
BsiHKAI G_wGCw'C

Cuts at:      0      38     719     875    1125 
   Size:         38     681     156     250

  Fragments arranged by size: 

                 681     250     156      38
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1036    1125 
   Size:       1036      89
 
Bsp1286I G_dGCh'C

Cuts at:      0      38     484     719     875     896    1125 
   Size:         38     446     235     156      21     229

  Fragments arranged by size: 

                 446     235     229     156      38      21
 
BspHI T'CATG_A

Cuts at:      0     200    1125 
   Size:        200     925
 
BssSI C'ACGA_G

Cuts at:      0    1002    1125 
   Size:       1002     123
 
BstEII G'GTnAC_C

Cuts at:      0     591    1125 
   Size:        591     534
 
BstYI r'GATC_y

Cuts at:      0     644     757     764     920    1125 
   Size:        644     113       7     156     205

  Fragments arranged by size: 

                 644     205     156     113       7
 
BtsI GCAGTG_nn'

Cuts at:      0    1070    1125 
   Size:       1070      55
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     600    1125 
   Size:        600     525
 
EaeI y'GGCC_r

Cuts at:      0      60     375     605    1125 
   Size:         60     315     230     520

  Fragments arranged by size: 

                 520     315     230      60
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     757    1125 
   Size:        757     368
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      67    1125 
   Size:         67    1058
 
EcoRI G'AATT_C

Cuts at:      0     418     628    1125 
   Size:        418     210     497

  Fragments arranged by size: 

                 497     418     210
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     705    1125 
   Size:        705     420
 
HaeII r_GCGC'y

Cuts at:      0      46     372    1125 
   Size:         46     326     753

  Fragments arranged by size: 

                 753     326      46
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     151     183     749     781    1125 
   Size:        151      32     566      32     344

  Fragments arranged by size: 

                 566     344     151      32      32
 
HincII GTy'rAC

Cuts at:      0     383     393    1125 
   Size:        383      10     732

  Fragments arranged by size: 

                 732     383      10
 
MscI TGG'CCA

Cuts at:      0      62    1125 
   Size:         62    1063
 
MslI CAynn'nnrTG

Cuts at:      0     722     868    1027    1125 
   Size:        722     146     159      98

  Fragments arranged by size: 

                 722     159     146      98
 
MspA1I CmG'CkG

Cuts at:      0     425     446     783    1125 
   Size:        425      21     337     342

  Fragments arranged by size: 

                 425     342     337      21
 
PasI CC'CwG_GG

Cuts at:      0     928    1125 
   Size:        928     197
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     655     726    1125 
   Size:        655      71     399

  Fragments arranged by size: 

                 655     399      71
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     895     927    1125 
   Size:        895      32     198

  Fragments arranged by size: 

                 895     198      32
 
PshAI GACnn'nnGTC

Cuts at:      0     388    1125 
   Size:        388     737
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     506     538    1125 
   Size:        506      32     587

  Fragments arranged by size: 

                 587     506      32
 
PstI C_TGCA'G

Cuts at:      0     445     460     739    1125 
   Size:        445      15     279     386

  Fragments arranged by size: 

                 445     386     279      15
 
PvuII CAG'CTG

Cuts at:      0     425     783    1125 
   Size:        425     358     342

  Fragments arranged by size: 

                 425     358     342
 
SfcI C'TryA_G

Cuts at:      0     272     441     456     735    1125 
   Size:        272     169      15     279     390

  Fragments arranged by size: 

                 390     279     272     169      15
 
StuI AGG'CCT

Cuts at:      0     948    1125 
   Size:        948     177
 
StyI C'CwwG_G

Cuts at:      0     638    1125 
   Size:        638     487
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     293     420    1125 
   Size:        293     127     705

  Fragments arranged by size: 

                 705     293     127
 
Tth111I GACn'n_nGTC

Cuts at:      0     192    1125 
   Size:        192     933
 

 Enzymes that do cut:

     AccI     AfeI     AleI     AloI    AlwNI    ApaLI     ApoI    BanII
     BbsI    BbvCI    BglII Bme1580I     BmrI     BpmI   Bpu10I    BsaAI
    BsaXI    BseYI     BsgI  BsiHKAI    BsmBI Bsp1286I    BspHI    BssSI
   BstEII    BstYI     BtsI     DrdI     EaeI     EciI  Eco57MI    EcoRI
     FalI    HaeII    Hin4I   HincII     MscI     MslI   MspA1I     PasI
    PflMI     PpiI    PshAI     PsrI     PstI    PvuII     SfcI     StuI
     StyI    TaqII  Tth111I

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AlfI     ApaI     AscI     AseI    AsiSI     AvaI    AvrII
     BaeI    BamHI     BanI     BbeI     BcgI    BciVI     BclI    BfrBI
     BglI     BlpI    BmgBI     BmtI     BplI    BpuEI     BsaI    BsaBI
    BsaHI    BsaWI    BseRI    BsiEI    BsiWI     BsmI    BspEI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII   BstAPI    BstBI    BstXI
  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII
     EagI     EarI  EcoICRI    EcoNI EcoO109I    EcoRV     FseI     FspI
    FspAI  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PciI     PfoI     PmeI     PmlI    PpuMI
     PsiI   PspOMI    PspXI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     SwaI     TatI     TstI     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI
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