Restriction map for pF1KE1050
 (Circular) (Six-base) MAPSORT of: pF1KE1050.seq  Check: 6897  from: 1  to: 4541



pF1KE1050  4541 bp

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:19  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   1207    1207 
   Size:       4541
 
AccI GT'mk_AC

Cuts at:   1579    1579 
   Size:       4541
 
Acc65I G'GTAC_C

Cuts at:   1557    1557 
   Size:       4541
 
AclI AA'CG_TT

Cuts at:   4381    4381 
   Size:       4541
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    535    2366    2798    3545    3813     535 
   Size:       1831     432     747     268    1263

  Fragments arranged by size: 

                1831    1263     747     432     268
 
AflIII A'CryG_T

Cuts at:   1893    2997    3974    1893 
   Size:       1104     977    2460

  Fragments arranged by size: 

                2460    1104     977
 
AgeI A'CCGG_T

Cuts at:   2929    2929 
   Size:       4541
 
AleI CACnn'nnGTG

Cuts at:   1201    1201 
   Size:       4541
 
AlwNI CAG_nnn'CTG

Cuts at:   1493    1846    3413    1493 
   Size:        353    1567    2621

  Fragments arranged by size: 

                2621    1567     353
 
ApaI G_GGCC'C

Cuts at:    119     562     689     700     839    1044     119 
   Size:        443     127      11     139     205    3616

  Fragments arranged by size: 

                3616     443     205     139     127      11
 
ApaLI G'TGCA_C

Cuts at:   3311    3311 
   Size:       4541
 
ApoI r'AATT_y

Cuts at:   1545    3894    4150    1545 
   Size:       2349     256    1936

  Fragments arranged by size: 

                2349    1936     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4541
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4541
 
AvaI C'yCGr_G

Cuts at:    189     693    1561     189 
   Size:        504     868    3169

  Fragments arranged by size: 

                3169     868     504
 
BamHI G'GATC_C

Cuts at:   1154    1566    1154 
   Size:        412    4129
 
BanI G'GyrC_C

Cuts at:    184     843    1557    1971    2219    2254     184 
   Size:        659     714     414     248      35    2471

  Fragments arranged by size: 

                2471     714     659     414     248      35
 
BanII G_rGCy'C

Cuts at:    119     149     159     350     482     511     562     689     700 
   Size:         30      10     191     132      29      51     127      11

Cuts at:    700     839    1044    1511    1555    2585     119 
   Size:        139     205     467      44    1030    2075

  Fragments arranged by size: 

                2075    1030     467     205     191     139     132     127
                  51      44      30      29      11      10
 
BbeI G_GCGC'C

Cuts at:   1975    2223    1975 
   Size:        248    4293
 
BciVI GTATCCnnnnn_n'

Cuts at:   2439    3200    4541    2439 
   Size:        761    1341    2439

  Fragments arranged by size: 

                2439    1341     761
 
BclI T'GATC_A

Cuts at:   2061    2061 
   Size:       4541
 
BglI GCCn_nnn'nGGC

Cuts at:    123     676    1226     123 
   Size:        553     550    3438

  Fragments arranged by size: 

                3438     553     550
 
BglII A'GATC_T

Cuts at:   2056    4093    2056 
   Size:       2037    2504
 
BlpI GC'TnA_GC

Cuts at:    406    1653     406 
   Size:       1247    3294
 
Bme1580I G_kGCm'C

Cuts at:    119     562     689     700     839     848    1044    1469    2166 
   Size:        443     127      11     139       9     196     425     697

Cuts at:   2166    2259    3315     119 
   Size:         93    1056    1345

  Fragments arranged by size: 

                1345    1056     697     443     425     196     139     127
                  93      11       9
 
BmrI ACTGGGnnnn_n'

Cuts at:   1753    1834    2169    4313    1753 
   Size:         81     335    2144    1981

  Fragments arranged by size: 

                2144    1981     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    235     875    2908     235 
   Size:        640    2033    1868

  Fragments arranged by size: 

                2033    1868     640
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    205     316     551     845    1135     205 
   Size:        111     235     294     290    3611

  Fragments arranged by size: 

                3611     294     290     235     111
 
Bpu10I CC'TnA_GC

Cuts at:    850    1331     850 
   Size:        481    4060
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1717    2596    3088    3386    1717 
   Size:        879     492     298    2872

  Fragments arranged by size: 

                2872     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    801    4245     801 
   Size:       3444    1097
 
BsaAI yAC'GTr

Cuts at:   1011    1104    1130    1894    1011 
   Size:         93      26     764    3658

  Fragments arranged by size: 

                3658     764      93      26
 
BsaBI GATnn'nnATC

Cuts at:      6    2055    4092       6 
   Size:       2049    2037     455

  Fragments arranged by size: 

                2049    2037     455
 
BsaHI Gr'CG_yC

Cuts at:    389    1972    2220    2922    4418     389 
   Size:       1583     248     702    1496     512

  Fragments arranged by size: 

                1583    1496     702     512     248
 
BsaWI w'CCGG_w

Cuts at:    288    1151    1730    2251    2929    3203    3350    3761     288 
   Size:        863     579     521     678     274     147     411    1068

  Fragments arranged by size: 

                1068     863     678     579     521     411     274     147
            
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    947     977     947 
   Size:         30    4511
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    836    1092    1360    1416    1420     836 
   Size:        256     268      56       4    3957

  Fragments arranged by size: 

                3957     268     256      56       4
 
BseYI C'CCAG_C

Cuts at:    540     603     790     919     979    1043    1136    1966    2724 
   Size:         63     187     129      60      64      93     830     758

Cuts at:   2724    3301    3797     540 
   Size:        577     496    1284

  Fragments arranged by size: 

                1284     830     758     577     496     187     129      93
                  64      63      60
 
BsiEI CG_ry'CG

Cuts at:    104    2083    2129    3337     104 
   Size:       1979      46    1208    1308

  Fragments arranged by size: 

                1979    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    833    1135    1511    1555    2333    2523    3315     833 
   Size:        302     376      44     778     190     792    2059

  Fragments arranged by size: 

                2059     792     778     376     302     190      44
 
BsmBI CGTCTCn'nnnn_

Cuts at:    551     551 
   Size:       4541
 
Bsp1286I G_dGCh'C

Cuts at:    119     149     159     350     482     511     562     689     700 
   Size:         30      10     191     132      29      51     127      11

Cuts at:    700     833     839     848    1044    1135    1469    1511    1555 
   Size:        133       6       9     196      91     334      42      44

Cuts at:   1555    2166    2259    2333    2523    2585    3315     119 
   Size:        611      93      74     190      62     730    1345

  Fragments arranged by size: 

                1345     730     611     334     196     191     190     133
                 132     127      93      91      74      62      51      44
                  42      30      29      11      10       9       6
 
BspEI T'CCGG_A

Cuts at:   3761    3761 
   Size:       4541
 
BspHI T'CATG_A

Cuts at:   3717    3717 
   Size:       4541
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1207    1591    1805    2107    2488    1207 
   Size:        384     214     302     381    3260

  Fragments arranged by size: 

                3260     384     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50     537    2833    2887    4371    4536      50 
   Size:        487    2296      54    1484     165      55

  Fragments arranged by size: 

                2296    1484     487     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2453    2453 
   Size:       4541
 
BsrFI r'CCGG_y

Cuts at:    201     303    2539    2720    2929     201 
   Size:        102    2236     181     209    1813

  Fragments arranged by size: 

                2236    1813     209     181     102
 
BssSI C'ACGA_G

Cuts at:   2812    3170    2812 
   Size:        358    4183
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1484    1918    1484 
   Size:        434    4107
 
BstBI TT'CG_AA

Cuts at:   2902    2902 
   Size:       4541
 
BstXI CCAn_nnnn'nTGG

Cuts at:    946     946 
   Size:       4541
 
BstYI r'GATC_y

Cuts at:      1    1154    1259    1566    2033    2056    2391    2637    3649 
   Size:       1153     105     307     467      23     335     246    1012

Cuts at:   3649    3735    3747    4093       1 
   Size:         86      12     346     449

  Fragments arranged by size: 

                1153    1012     467     449     346     335     307     246
                 105      86      23      12
 
Bsu36I CC'TnA_GG

Cuts at:    161     161 
   Size:       4541
 
BtgI C'CryG_G

Cuts at:    180     207     321    1070    1215     180 
   Size:         27     114     749     145    3506

  Fragments arranged by size: 

                3506     749     145     114      27
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2491    2672    2835    2491 
   Size:        181     163    4197

  Fragments arranged by size: 

                4197     181     163
 
BtsI GCAGTG_nn'

Cuts at:    271    1263    1784     271 
   Size:        992     521    3028

  Fragments arranged by size: 

                3028     992     521
 
DraI TTT'AAA

Cuts at:   1540    1540 
   Size:       4541
 
DrdI GACnn_nn'nnGTC

Cuts at:   2247    3105    2247 
   Size:        858    3683
 
EaeI y'GGCC_r

Cuts at:    419     936    1053    1106    2126    2300    2691    2718    4063 
   Size:        517     117      53    1020     174     391      27    1345

Cuts at:   4063     419 
   Size:        897

  Fragments arranged by size: 

                1345    1020     897     517     391     174     117      53
                  27
 
EagI C'GGCC_G

Cuts at:   2126    2126 
   Size:       4541
 
EarI CTCTTCn'nnn_

Cuts at:    807     813    2564    2774     807 
   Size:          6    1751     210    2574

  Fragments arranged by size: 

                2574    1751     210       6
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2749    3059    3205    4351    2749 
   Size:        310     146    1146    2939

  Fragments arranged by size: 

                2939    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   1509    1553    1509 
   Size:         44    4497
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    205     316     535     551     845    1135    2366    2798    3545 
   Size:        111     219      16     294     290    1231     432     747

Cuts at:   3545    3813     205 
   Size:        268     933

  Fragments arranged by size: 

                1231     933     747     432     294     290     268     219
                 111      16
 
EcoNI CCTnn'n_nnAGG

Cuts at:    713    1334     713 
   Size:        621    3920
 
EcoO109I rG'GnC_Cy

Cuts at:    115     276     317     366     558     559     686     697     736 
   Size:        161      41      49     192       1     127      11      39

Cuts at:    736     836    1680     115 
   Size:        100     844    2976

  Fragments arranged by size: 

                2976     844     192     161     127     100      49      41
                  39      11       1
 
EcoRI G'AATT_C

Cuts at:   1545    1545 
   Size:       4541
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2560    2560 
   Size:       4541
 
FspI TGC'GCA

Cuts at:   2322    2322 
   Size:       4541
 
HaeII r_GCGC'y

Cuts at:   1493    1975    2223    3245    4224    1493 
   Size:        482     248    1022     979    1810

  Fragments arranged by size: 

                1810    1022     979     482     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    713     745    2383    2413    2415    2445     713 
   Size:         32    1638      30       2      30    2809

  Fragments arranged by size: 

                2809    1638      32      30      30       2
 
HincII GTy'rAC

Cuts at:   1580    1580 
   Size:       4541
 
KasI G'GCGC_C

Cuts at:   1971    2219    1971 
   Size:        248    4293
 
KpnI G_GTAC'C

Cuts at:   1561    1561 
   Size:       4541
 
MluI A'CGCG_T

Cuts at:   3974    3974 
   Size:       4541
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   3212    3396    3212 
   Size:        184    4357
 
MscI TGG'CCA

Cuts at:    938    1108    2302     938 
   Size:        170    1194    3177

  Fragments arranged by size: 

                3177    1194     170
 
MslI CAynn'nnrTG

Cuts at:   1201    2530    2657    1201 
   Size:       1329     127    3085

  Fragments arranged by size: 

                3085    1329     127
 
MspA1I CmG'CkG

Cuts at:    182     923    1217    1458    1652    1966    2326    3339    3584 
   Size:        741     294     241     194     314     360    1013     245

Cuts at:   3584     182 
   Size:       1139

  Fragments arranged by size: 

                1139    1013     741     360     314     294     245     241
                 194
 
NaeI GCC'GGC

Cuts at:    305    2722     305 
   Size:       2417    2124
 
NarI GG'CG_CC

Cuts at:   1972    2220    1972 
   Size:        248    4293
 
NcoI C'CATG_G

Cuts at:    321     321 
   Size:       4541
 
NdeI CA'TA_TG

Cuts at:    474     474 
   Size:       4541
 
NgoMIV G'CCGG_C

Cuts at:    303    2720     303 
   Size:       2417    2124
 
NspI r_CATG'y

Cuts at:   1037    1594    2094    3001    1037 
   Size:        557     500     907    2577

  Fragments arranged by size: 

                2577     907     557     500
 
PasI CC'CwG_GG

Cuts at:    233     280     233 
   Size:         47    4494
 
PciI A'CATG_T

Cuts at:   2997    2997 
   Size:       4541
 
PfoI T'CCnGG_A

Cuts at:   1145    1502    1145 
   Size:        357    4184
 
PmeI GTTT'AAAC

Cuts at:   1540    1540 
   Size:       4541
 
PmlI CAC'GTG

Cuts at:   1011    1130    1011 
   Size:        119    4422
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3680    3712    3680 
   Size:         32    4509
 
PpuMI rG'GwC_Cy

Cuts at:    317     317 
   Size:       4541
 
PspOMI G'GGCC_C

Cuts at:    115     558     685     696     835    1040     115 
   Size:        443     127      11     139     205    3616

  Fragments arranged by size: 

                3616     443     205     139     127      11
 
PstI C_TGCA'G

Cuts at:    357     810    1489    1588    2273     357 
   Size:        453     679      99     685    2625

  Fragments arranged by size: 

                2625     685     679     453      99
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4541
 
PvuII CAG'CTG

Cuts at:   1966    2326    1966 
   Size:        360    4181
 
RsrII CG'GwC_CG

Cuts at:   2736    2736 
   Size:       4541
 
SacI G_AGCT'C

Cuts at:   1511    1555    1511 
   Size:         44    4497
 
SacII CC_GC'GG

Cuts at:    183    1218     183 
   Size:       1035    3506
 
SalI G'TCGA_C

Cuts at:   1578    1578 
   Size:       4541
 
SanDI GG'GwC_CC

Cuts at:    317     317 
   Size:       4541
 
SapI GCTCTTCn'nnn_

Cuts at:   2564    2774    2564 
   Size:        210    4331
 
SbfI CC_TGCA'GG

Cuts at:    810    1588     810 
   Size:        778    3763
 
ScaI AGT'ACT

Cuts at:    329     931    4058     329 
   Size:        602    3127     812

  Fragments arranged by size: 

                3127     812     602
 
SfcI C'TryA_G

Cuts at:     33     353     744     806    1485    1584    2269    3262    3453 
   Size:        320     391      62     679      99     685     993     191

Cuts at:   3453      33 
   Size:       1121

  Fragments arranged by size: 

                1121     993     685     679     391     320     191      99
                  62
 
SfoI GGC'GCC

Cuts at:   1973    2221    1973 
   Size:        248    4293
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4541
 
SmaI CCC'GGG

Cuts at:    191     695    1563     191 
   Size:        504     868    3169

  Fragments arranged by size: 

                3169     868     504
 
SmlI C'TyrA_G

Cuts at:   1696    2611    3103    3365    1696 
   Size:        915     492     262    2872

  Fragments arranged by size: 

                2872     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4541
 
SphI G_CATG'C

Cuts at:   1594    2094    1594 
   Size:        500    4041
 
StuI AGG'CCT

Cuts at:    679    1177    1483     679 
   Size:        498     306    3737

  Fragments arranged by size: 

                3737     498     306
 
StyI C'CwwG_G

Cuts at:    321    1675    2029     321 
   Size:       1354     354    2833

  Fragments arranged by size: 

                2833    1354     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    300     479     672    2124    2257    2716    2925     300 
   Size:        179     193    1452     133     459     209    1916

  Fragments arranged by size: 

                1916    1452     459     209     193     179     133
 
TatI w'GTAC_w

Cuts at:    327     929    4056     327 
   Size:        602    3127     812

  Fragments arranged by size: 

                3127     812     602
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    947     979    4058    4090     947 
   Size:         32    3079      32    1398

  Fragments arranged by size: 

                3079    1398      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   2338    2338 
   Size:       4541
 
XbaI T'CTAG_A

Cuts at:   1572    1572 
   Size:       4541
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2036    2036 
   Size:       4541
 
XmaI C'CCGG_G

Cuts at:    189     693    1561     189 
   Size:        504     868    3169

  Fragments arranged by size: 

                3169     868     504
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AleI
    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI
     BanI    BanII     BbeI    BciVI     BclI     BglI    BglII     BlpI
 Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI
    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BssSI   BstAPI    BstBI    BstXI    BstYI   Bsu36I     BtgI    BtgZI
     BtsI     DraI     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I
   HincII     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NspI     PasI     PciI
     PfoI     PmeI     PmlI     PpiI    PpuMI   PspOMI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI
     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XmaI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AhdI     AlfI     AloI     AscI    AvrII
     BaeI     BbsI    BbvCI     BcgI    BfrBI    BmgBI     BmtI     BsgI
    BsiWI     BsmI    BsrGI   BssHII   BstEII  BstZ17I     ClaI    CspCI
   DraIII    EcoRV     FseI    FspAI  HindIII     HpaI     MfeI     NheI
     NotI     NruI     NsiI     PacI    PflMI    PshAI     PsiI    PspXI
     PsrI    SexAI     SfiI    SgrAI    SnaBI     SrfI     SspI     SwaI
     XhoI     XmnI     ZraI
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