Restriction map for pF1KE1043
##################################
# NO CUT ENZYMES (  120 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
AflIII      AgeI        AhdI        Alw44I      Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp718I     AspEI       AspI        AviII       AvrII       
BamHI       BciVI       BclI        BfaI        BfrI        
BglI        BlnI        BsaBI       BsaWI       BseAI       
BsiEI       BsiWI       BsmBI       BsmFI       BspDI       
BspEI       BspHI       BspLU11I    BsrBI       BssHII      
BssSI       Bst1107I    BstEII      BstPI       BstZ17I     
ClaI        CpoI        DraI        DraIII      DrdI        
DsaI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      EcoNI       EcoO65I     EcoRI       
EcoRV       EcoT22I     FbaI        FseI        FspI        
HgaI        HincII      HindII      HindIII     HpaI        
KpnI        Ksp632I     KspI        MaeI        MamI        
MfeI        MluI        MroI        MseI        MunI        
NcoI        NdeI        NheI        NotI        NruI        
NsiI        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmeI        PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacI        SacII       
SalI        SapI        ScaI        SexAI       SfiI        
SgrAI       SmaI        SnaBI       SpeI        SspI        
SwaI        TfiI        Tru9I       Tth111I     Van91I      
XbaI        XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   59 enzymes) #
###################################

AccI          493  GTCTAC               GT^[AC][GT]AC
AccII          76  CGCG                 CG^CG
AcsI         1245  GAATTT               [GA]^AATT[CT]
AcyI          410  GGCGCC               G[GA]^CG[CT]C
AlwNI         868  CAGGAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          163  CCTGAGG              CC^T[ACGT]AGG
ApoI         1245  GAATTT               [GA]^AATT[CT]
Asp700I      1242  GAAGAATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI          692  CTCGGG               C^[CT]CG[GA]G
BalI          833  TGGCCA               TGG^CCA
BanII        1272  GGGCTC               G[GA]GC[CT]^C
BbeI          410  GGCGCC               GGCGC^C
BbrPI        1263  CACGTG               CAC^GTG
BbvCI        1404  CCTCAGC              CCTCAGC(-5/-2)
BcgI          876  GCAGGAGCTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII         194  AGATCT               A^GATCT
BlpI          360  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      360  GCTGAGC              GC^T[ACGT]AGC
BsaAI        1263  CACGTG               [CT]AC^GT[GA]
BsaHI         410  GGCGCC               G[GA]^CG[CT]C
BsaI         1216  GAGACC               GGTCTC(1/5)
BsmAI        1216  GAGAC                GTCTC(1/5)
BsmI         1048  GCATTC               GAATGC(1/-1)
BsoBI         692  CTCGGG               C^[CT]CG[GA]G
Bsp1407I     1094  TGTACA               T^GTACA
BsrFI         857  GCCGGC               [GA]^CCGG[CT]
BsrGI        1094  TGTACA               T^GTACA
BstBI        1334  TTCGAA               TT^CGAA
BstUI          76  CGCG                 CG^CG
BstXI        1258  CCAGGCACGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        163  CCTGAGG              CC^T[ACGT]AGG
CelII         360  GCTGAGC              GC^T[ACGT]AGC
Cfr10I        857  GCCGGC               [GA]^CCGG[CT]
DraII         698  AGGACCT              [GA]G^G[ACGT]CC[CT]
Eco81I        163  CCTGAGG              CC^T[ACGT]AGG
EcoO109I      698  AGGACCT              [GA]G^G[ACGT]CC[CT]
HaeII         410  GGCGCC               [GA]GCGC^[CT]
Hin1I         410  GGCGCC               G[GA]^CG[CT]C
KasI          410  GGCGCC               G^GCGCC
MluNI         833  TGGCCA               TGG^CCA
MscI          833  TGGCCA               TGG^CCA
MvnI           76  CGCG                 CG^CG
NaeI          857  GCCGGC               GCC^GGC
NarI          410  GGCGCC               GG^CGCC
NgoMIV        857  GCCGGC               G^CCGGC
NspV         1334  TTCGAA               TT^CGAA
PmaCI        1263  CACGTG               CAC^GTG
PmlI         1263  CACGTG               CAC^GTG
PpuMI         698  AGGACCT              [GA]G^G[AT]CC[CT]
PvuII         723  CAGCTG               CAG^CTG
SbfI          126  CCTGCAGG             CCTGCA^GG
SfoI          410  GGCGCC               GGC^GCC
SfuI         1334  TTCGAA               TT^CGAA
SphI          546  GCATGC               GCATG^C
Sse8387I      126  CCTGCAGG             CCTGCA^GG
SspBI        1094  TGTACA               T^GTACA
StuI          452  AGGCCT               AGG^CCT
Tsp509I      1246  AATT                 ^AATT
XmnI         1242  GAAGAATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AlwI         1401  GATCC                GGATC(4/5)
             1173  GGATC               
AspHI         742  GTGCTC               G[AT]GC[AT]^C
              925  GTGCTC              
AvaII         168  GGACC                G^G[AT]CC
              699  GGACC               
BbsI          151  GTCTTC               GAAGAC(2/6)
             1366  GTCTTC              
BcnI          207  CCCGG                CC^[GC]GG
             1394  CCCGG               
BpuAI         151  GTCTTC               GAAGAC(2/6)
             1366  GTCTTC              
BsiHKAI       742  GTGCTC               G[AT]GC[AT]^C
              925  GTGCTC              
BsrDI         793  GCAATG               GCAATG(2/0)
             1061  GCAATG              
BstAPI        584  GCACCTACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              867  GCAGGAGCTGC         
BstYI         194  AGATCT               [GA]^GATC[CT]
             1400  AGATCC              
EaeI          102  CGGCCA               [CT]^GGCC[GA]
              833  TGGCCA              
EcoT14I       643  CCAAGG               C^C[AT][AT]GG
              971  CCAAGG              
HinfI         149  GAGTC                G^A[ACGT]TC
              760  GACTC               
MflI          194  AGATCT               [GA]^GATC[CT]
             1400  AGATCC              
MslI         1018  CATAAGTATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1098  CATTCGTGTG          
NciI          207  CCCGG                CC^[GC]GG
             1394  CCCGG               
NspI          546  GCATGC               [GA]CATG^[CT]
              804  GCATGT              
PleI          760  GACTC                GAGTC(4/5)
              149  GAGTC               
StyI          643  CCAAGG               C^C[AT][AT]GG
              971  CCAAGG              
TaqI           11  TCGA                 T^CGA
             1335  TCGA                
TthHB8I        11  TCGA                 T^CGA
             1335  TCGA                
XhoII         194  AGATCT               [GA]^GATC[CT]
             1400  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   57 enzymes) #
#########################################

AciI        AfaI        AluI        BanI        BbvI        
BmyI        BpmI        BsaJI       BseRI       BsgI        
BsiYI       BslI        Bsp1286I    BspMI       BsrI        
BssKI       BstF5I      BstNI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       EcoRII      FauI        
Fnu4HI      FokI        HaeIII      HapII       HhaI        
HinP1I      HpaII       HphI        ItaI        MaeII       
MaeIII      MboI        MboII       MnlI        MspA1I      
MspI        MvaI        MwoI        NdeII       NlaIII      
NlaIV       PstI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        TseI        
Tsp45I      TspRI       
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