Restriction map for
pF1KE1043
(Circular) (Six-base) MAPSORT of: pF1KE1043.seq Check: 4041 from: 1 to: 4523
pF1KE1043 4523 bp
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 214 1137 214
Size: 923 3600
AccI GT'mk_AC
Cuts at: 602 1561 602
Size: 959 3564
Acc65I G'GTAC_C
Cuts at: 1539 1539
Size: 4523
AclI AA'CG_TT
Cuts at: 4363 4363
Size: 4523
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 534 844 1089 2348 2780 3527 3795 534
Size: 310 245 1259 432 747 268 1262
Fragments arranged by size:
1262 1259 747 432 310 268 245
AflIII A'CryG_T
Cuts at: 1875 2979 3956 1875
Size: 1104 977 2442
Fragments arranged by size:
2442 1104 977
AgeI A'CCGG_T
Cuts at: 2911 2911
Size: 4523
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1169 1169
Size: 4523
AlwNI CAG_nnn'CTG
Cuts at: 981 1828 3395 981
Size: 847 1567 2109
Fragments arranged by size:
2109 1567 847
ApaLI G'TGCA_C
Cuts at: 3293 3293
Size: 4523
ApoI r'AATT_y
Cuts at: 1353 1527 3876 4132 1353
Size: 174 2349 256 1744
Fragments arranged by size:
2349 1744 256 174
AseI AT'TA_AT
Cuts at: 20 20
Size: 4523
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4523
AvaI C'yCGr_G
Cuts at: 800 1543 800
Size: 743 3780
BamHI G'GATC_C
Cuts at: 1548 1548
Size: 4523
BanI G'GyrC_C
Cuts at: 518 622 887 1222 1539 1953 2201 2236 518
Size: 104 265 335 317 414 248 35 2805
Fragments arranged by size:
2805 414 335 317 265 248 104 35
BanII G_rGCy'C
Cuts at: 1384 1537 2567 1384
Size: 153 1030 3340
Fragments arranged by size:
3340 1030 153
BbeI G_GCGC'C
Cuts at: 522 1957 2205 522
Size: 1435 248 2840
Fragments arranged by size:
2840 1435 248
BbsI GAAGACnn'nnnn_
Cuts at: 252 1467 252
Size: 1215 3308
BbvCI CC'TCA_GC
Cuts at: 1513 1513
Size: 4523
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 973 1007 90
Size: 34 849 34 3606
Fragments arranged by size:
3606 849 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2421 3182 4523 2421
Size: 761 1341 2421
Fragments arranged by size:
2421 1341 761
BclI T'GATC_A
Cuts at: 2043 2043
Size: 4523
BglII A'GATC_T
Cuts at: 302 2038 4075 302
Size: 1736 2037 750
Fragments arranged by size:
2037 1736 750
BlpI GC'TnA_GC
Cuts at: 469 1635 469
Size: 1166 3357
Bme1580I G_kGCm'C
Cuts at: 163 271 589 627 673 751 793 892 957
Size: 108 318 38 46 78 42 99 65
Cuts at: 957 1189 2148 2241 3297 163
Size: 232 959 93 1056 1389
Fragments arranged by size:
1389 1056 959 318 232 108 99 93
78 65 46 42 38
BmgBI CAC'GTC
Cuts at: 1317 1317
Size: 4523
BmrI ACTGGGnnnn_n'
Cuts at: 1735 1816 2151 4295 1735
Size: 81 335 2144 1963
Fragments arranged by size:
2144 1963 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2890 2890
Size: 4523
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 154 402 1024 154
Size: 248 622 3653
Fragments arranged by size:
3653 622 248
Bpu10I CC'TnA_GC
Cuts at: 893 1513 893
Size: 620 3903
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 508 1117 1261 1699 2578 3070 3368 508
Size: 609 144 438 879 492 298 1663
Fragments arranged by size:
1663 879 609 492 438 298 144
BsaI GGTCTCn'nnnn_
Cuts at: 1318 4227 1318
Size: 2909 1614
BsaAI yAC'GTr
Cuts at: 1373 1876 1373
Size: 503 4020
BsaBI GATnn'nnATC
Cuts at: 6 2037 4074 6
Size: 2031 2037 455
Fragments arranged by size:
2037 2031 455
BsaHI Gr'CG_yC
Cuts at: 519 1954 2202 2904 4400 519
Size: 1435 248 702 1496 642
Fragments arranged by size:
1496 1435 702 642 248
BsaWI w'CCGG_w
Cuts at: 1712 2233 2911 3185 3332 3743 1712
Size: 521 678 274 147 411 2492
Fragments arranged by size:
2492 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 382 934 1021 382
Size: 552 87 3884
Fragments arranged by size:
3884 552 87
BseYI C'CCAG_C
Cuts at: 650 1110 1948 2706 3283 3779 650
Size: 460 838 758 577 496 1394
Fragments arranged by size:
1394 838 758 577 496 460
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 214 473 675 684 214
Size: 259 202 9 4053
Fragments arranged by size:
4053 259 202 9
BsiEI CG_ry'CG
Cuts at: 104 2065 2111 3319 104
Size: 1961 46 1208 1308
Fragments arranged by size:
1961 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 854 1037 1537 2315 2505 3297 854
Size: 183 500 778 190 792 2080
Fragments arranged by size:
2080 792 778 500 190 183
BsmI GAATG_Cn'
Cuts at: 1156 1156
Size: 4523
Bsp1286I G_dGCh'C
Cuts at: 163 271 589 627 673 751 793 854 892
Size: 108 318 38 46 78 42 61 38
Cuts at: 892 957 1037 1189 1384 1537 2148 2241 2315
Size: 65 80 152 195 153 611 93 74
Cuts at: 2315 2505 2567 3297 163
Size: 190 62 730 1389
Fragments arranged by size:
1389 730 611 318 195 190 153 152
108 93 80 78 74 65 62 61
46 42 38 38
BspEI T'CCGG_A
Cuts at: 3743 3743
Size: 4523
BspHI T'CATG_A
Cuts at: 3699 3699
Size: 4523
BspMI ACCTGCnnnn'nnnn_
Cuts at: 186 214 238 430 495 818 1137 1573 1787
Size: 28 24 192 65 323 319 436 214
Cuts at: 1787 2089 2470 186
Size: 302 381 2239
Fragments arranged by size:
2239 436 381 323 319 302 214 192
65 28 24
BsrBI CCG'CTC
Cuts at: 50 2815 2869 4353 4518 50
Size: 2765 54 1484 165 55
Fragments arranged by size:
2765 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 908 1176 2435 908
Size: 268 1259 2996
Fragments arranged by size:
2996 1259 268
BsrFI r'CCGG_y
Cuts at: 965 2521 2702 2911 965
Size: 1556 181 209 2577
Fragments arranged by size:
2577 1556 209 181
BsrGI T'GTAC_A
Cuts at: 1202 1202
Size: 4523
BssSI C'ACGA_G
Cuts at: 2794 3152 2794
Size: 358 4165
BstAPI GCAn_nnn'nTGC
Cuts at: 698 981 1900 698
Size: 283 919 3321
Fragments arranged by size:
3321 919 283
BstBI TT'CG_AA
Cuts at: 1443 2884 1443
Size: 1441 3082
BstXI CCAn_nnnn'nTGG
Cuts at: 1373 1373
Size: 4523
BstYI r'GATC_y
Cuts at: 1 302 1508 1548 2015 2038 2373 2619 3631
Size: 301 1206 40 467 23 335 246 1012
Cuts at: 3631 3717 3729 4075 1
Size: 86 12 346 449
Fragments arranged by size:
1206 1012 467 449 346 335 301 246
86 40 23 12
Bsu36I CC'TnA_GG
Cuts at: 272 272
Size: 4523
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2473 2654 2817 2473
Size: 181 163 4179
Fragments arranged by size:
4179 181 163
BtsI GCAGTG_nn'
Cuts at: 1029 1182 1766 1029
Size: 153 584 3786
Fragments arranged by size:
3786 584 153
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 762 797 1158 1193 762
Size: 35 361 35 4092
Fragments arranged by size:
4092 361 35 35
DraI TTT'AAA
Cuts at: 1522 1522
Size: 4523
DrdI GACnn_nn'nnGTC
Cuts at: 2229 3087 2229
Size: 858 3665
EaeI y'GGCC_r
Cuts at: 210 941 2108 2282 2673 2700 4045 210
Size: 731 1167 174 391 27 1345 688
Fragments arranged by size:
1345 1167 731 688 391 174 27
EagI C'GGCC_G
Cuts at: 2108 2108
Size: 4523
EarI CTCTTCn'nnn_
Cuts at: 2546 2756 2546
Size: 210 4313
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2731 3041 3187 4333 2731
Size: 310 146 1146 2921
Fragments arranged by size:
2921 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1535 1535
Size: 4523
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 154 402 534 844 1024 1089 2348 2780 3527
Size: 248 132 310 180 65 1259 432 747
Cuts at: 3527 3795 154
Size: 268 882
Fragments arranged by size:
1259 882 747 432 310 268 248 180
132 65
EcoO109I rG'GnC_Cy
Cuts at: 807 1662 807
Size: 855 3668
EcoRI G'AATT_C
Cuts at: 1527 1527
Size: 4523
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2542 2542
Size: 4523
FspI TGC'GCA
Cuts at: 2304 2304
Size: 4523
HaeII r_GCGC'y
Cuts at: 522 1957 2205 3227 4206 522
Size: 1435 248 1022 979 839
Fragments arranged by size:
1435 1022 979 839 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 997 1029 2365 2395 2397 2427 997
Size: 32 1336 30 2 30 3093
Fragments arranged by size:
3093 1336 32 30 30 2
HincII GTy'rAC
Cuts at: 1562 1562
Size: 4523
KasI G'GCGC_C
Cuts at: 518 1953 2201 518
Size: 1435 248 2840
Fragments arranged by size:
2840 1435 248
KpnI G_GTAC'C
Cuts at: 1543 1543
Size: 4523
MluI A'CGCG_T
Cuts at: 3956 3956
Size: 4523
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3194 3378 3194
Size: 184 4339
MscI TGG'CCA
Cuts at: 943 2284 943
Size: 1341 3182
MslI CAynn'nnrTG
Cuts at: 1130 1210 2512 2639 1130
Size: 80 1302 127 3014
Fragments arranged by size:
3014 1302 127 80
MspA1I CmG'CkG
Cuts at: 353 380 833 1634 1948 2308 3321 3566 353
Size: 27 453 801 314 360 1013 245 1310
Fragments arranged by size:
1310 1013 801 453 360 314 245 27
NaeI GCC'GGC
Cuts at: 967 2704 967
Size: 1737 2786
NarI GG'CG_CC
Cuts at: 519 1954 2202 519
Size: 1435 248 2840
Fragments arranged by size:
2840 1435 248
NgoMIV G'CCGG_C
Cuts at: 965 2702 965
Size: 1737 2786
NspI r_CATG'y
Cuts at: 658 916 1576 2076 2983 658
Size: 258 660 500 907 2198
Fragments arranged by size:
2198 907 660 500 258
PasI CC'CwG_GG
Cuts at: 1294 1294
Size: 4523
PciI A'CATG_T
Cuts at: 2979 2979
Size: 4523
PfoI T'CCnGG_A
Cuts at: 131 131
Size: 4523
PmeI GTTT'AAAC
Cuts at: 1522 1522
Size: 4523
PmlI CAC'GTG
Cuts at: 1373 1373
Size: 4523
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3662 3694 3662
Size: 32 4491
PpuMI rG'GwC_Cy
Cuts at: 807 807
Size: 4523
PstI C_TGCA'G
Cuts at: 239 427 986 1148 1570 2255 239
Size: 188 559 162 422 685 2507
Fragments arranged by size:
2507 685 559 422 188 162
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4523
PvuII CAG'CTG
Cuts at: 833 1948 2308 833
Size: 1115 360 3048
Fragments arranged by size:
3048 1115 360
RsrII CG'GwC_CG
Cuts at: 2718 2718
Size: 4523
SacI G_AGCT'C
Cuts at: 1537 1537
Size: 4523
SalI G'TCGA_C
Cuts at: 1560 1560
Size: 4523
SapI GCTCTTCn'nnn_
Cuts at: 2546 2756 2546
Size: 210 4313
SbfI CC_TGCA'GG
Cuts at: 239 1570 239
Size: 1331 3192
ScaI AGT'ACT
Cuts at: 4040 4040
Size: 4523
SfcI C'TryA_G
Cuts at: 33 235 403 423 982 1069 1144 1566 2251
Size: 202 168 20 559 87 75 422 685
Cuts at: 2251 3244 3435 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 685 559 422 202 191 168
87 75 20
SfoI GGC'GCC
Cuts at: 520 1955 2203 520
Size: 1435 248 2840
Fragments arranged by size:
2840 1435 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4523
SmaI CCC'GGG
Cuts at: 1545 1545
Size: 4523
SmlI C'TyrA_G
Cuts at: 523 1096 1240 1678 2593 3085 3347 523
Size: 573 144 438 915 492 262 1699
Fragments arranged by size:
1699 915 573 492 438 262 144
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4523
SphI G_CATG'C
Cuts at: 658 1576 2076 658
Size: 918 500 3105
Fragments arranged by size:
3105 918 500
StuI AGG'CCT
Cuts at: 562 562
Size: 4523
StyI C'CwwG_G
Cuts at: 751 1079 1657 2011 751
Size: 328 578 354 3263
Fragments arranged by size:
3263 578 354 328
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2106 2239 2698 2907 2106
Size: 133 459 209 3722
Fragments arranged by size:
3722 459 209 133
TatI w'GTAC_w
Cuts at: 327 1202 4038 327
Size: 875 2836 812
Fragments arranged by size:
2836 875 812
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4040 4072 4040
Size: 32 4491
Tth111I GACn'n_nGTC
Cuts at: 2320 2320
Size: 4523
XbaI T'CTAG_A
Cuts at: 1554 1554
Size: 4523
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2018 2018
Size: 4523
XmaI C'CCGG_G
Cuts at: 1543 1543
Size: 4523
XmnI GAAnn'nnTTC
Cuts at: 1354 1354
Size: 4523
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgZI BtsI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PasI PciI
PfoI PmeI PmlI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI ApaI AscI
AvrII BaeI BfrBI BglI BmtI BsaXI BsiWI BsmBI
BssHII BstEII BstZ17I BtgI ClaI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PflMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI ZraI